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All Journal Jurnal Akuakultur Indonesia Teknotan: Jurnal Industri Teknologi Pertanian BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Jurnal Riset Kimia agriTECH PROSIDING SEMINAR KIMIA JURNAL ATOMIK Gorontalo Journal of Forestry Research Jurnal Jamu Indonesia PERENNIAL ULIN: Jurnal Hutan Tropis Jurnal Riset Teknologi Industri Journal Of Medicine And Health Sciences (Medisci) Journal of Tropical Pharmacy and Chemistry Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Indonesian Journal of Jamu

Irawan Wijaya Kusuma, Irawan Wijaya

Fakultas Kehutanan Universitas Mulawarman

Author-ID : 2473377

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health Veterinary Other

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AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI BAGIAN RANTING DAN BATANG TUMBUHAN KARAMUNTING (Melastoma malabathricum): Antioxidant and Antibacterial Activity of The Twigs and Stem Bark of Karamunting Plants (Melastoma malabathricum) Nur Maulida Sari; Irawan Wijaya Kusuma; Rudianto Amirta; Nur Indriana Fitriah
PERENNIAL Vol. 17 No. 2 (2021): Vol. 17 No. 2, Oktober 2021
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v17i2.14547

East Kalimantan is one of the areas that have the potential for various traditional medicinal plants. Melastoma malabathricum (known locally as karamunting, senduduk) is an invasive plant in the family of Melastomataceae with a potential medicinal plant. This study explored the potential of twigs and stem bark of Karamunting (Melastoma malabathricum) for its antioxidant and antibacterial activity. The twigs and stem bark of Karamunting were macerated to yield ethanolic extract. The phytochemical screening was evaluated by Harborne and Kokate methods. Antioxidant activity was evaluated by DPPH radical scavenging assay. Antibacterial activity was examined using agar well diffusion method against Propionibacterium acnes. The results showed that the ethanol extracts of twigs and stem bark samples display an ability to inhibit DPPH free radical by 82% and 88% at 50 ppm concentration. Antibacterial activity of the twigs and stem bark samples showed potent activity to inhibit the P. acnes growth with 11.3 mm and 11 mm inhibition activity. Based on the results, the twigs and stem bark of M. malabathricum plants display potential as a natural antioxidant and antibacterial agent.

Skrining Fitokimia dan Toksisitas dari Ekstrak Daun Pecut Kuda (Stachytarpheta jamaicenesis (L.) Vahl) Irawan Wijaya Kusuma; Erwin Erwin; Muhammad Wildan Rizqi Miftahussanadi
ULIN: Jurnal Hutan Tropis Vol 5, No 1 (2021)
Publisher : Fakultas Kehutanan Universitas Mulawarman

The Pecut kuda plant is one of the plants of the genus Stachytarpheta which is found in tropical regions, including Indonesia. In Indonesia this plant is found in East Kalimantan which grows wild. This study aims to determine the type of secondary metabolites and the level of toxicity of the leaves of horse whip (Stachytarpheta jamaicenesis (L.) Vahl). The research stages were sample drying, sample maceration, filtered, concentrated with a rotary evaporator, phytochemical and toxicity tests using linear regression values to determine the LC50 value with SAS probit analysis. Phytochemical test results showed that the pecut horse leaf extract in the n-hexane fraction contained flavonoids, quinones and steroids, the ethyl acetate fraction contained alkaloids, steroids, saponins, flavonoids and quinones and the methanol fraction contained phenolic flavonoids, quinones and saponins. The results of the toxicity test on Artemia salina L. shrimp larvae in the fraction of n-hexane, ethyl acetate and methanol obtained LC50 values of 0.036 ppm, 5.24 ppm and 29.51 ppm, respectively. Based on the LC50 value, it can be concluded that the three fractions are very toxic, but the most toxic is the n-hexane fraction (LC50 = 0.036 ppm).

POTENSI JAMUR TIRAM PUTIH (Pleurotus ostreatus) TERHADAP PENGHAMBATAN Candida albicans DAN Propionibacterium acnes Saat Egra; Irawan Wijaya Kusuma; Enos Tangke Arung
ULIN: Jurnal Hutan Tropis Vol 2, No 1 (2018)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Oyster mushroom (Pleurotus ostreatus) is not a new food for the community. Long time ago oyster mushrooms is food but not so well known. Now oyster mushrooms have been accepted by the community as a healthy food. This is the background of this research to produce mushrooms not only as nutritious food but also natural medicine. The purpose of this study was to examine the antimicrobial potential of oyster mushrooms by determination of clear zone against Candida albicans and Propionibacterium acnes bacterias. This research was conducted at Forest Products Chemistry Laboratory, Faculty of Forestry, Mulawarman University. The resources of oyster mushroom we used in this study comes from the cultivation of the entrepreanure forestry student group in Mulawarman University. This study used successful extraction with hexane solvent, ethyl acetate, ethanol, water, and crude ethanol. The results obtained that the highest antimicrobial inhibition against Candida albicans bacteria with 47.60% with a concentration of 100 ppm but, on the antimicrobial against Propionibacterium acnes bacteria did not show any significant inhibition.

Aktivitas Antioksidan, Kandungan Total Fenolik dan Kandungan Total Flavonoid Pada Beberapa Tumbuhan Berkhasiat Obat Di Kalimantan Timur, Indonesia Nur Maulida Sari; Irawan Wijaya Kusuma; Nur Indriana Fitriah
ULIN: Jurnal Hutan Tropis Vol 5, No 2 (2021)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v5i2.6224

Antioxidant activity, total phenolic and total flavonoid contents of some traditional medicinal plants grown in East Kalimantan, Indonesia, were investigated. Plant sample are Macaranga gigantea leaf, Ceiba pentandra leaf, Ceiba pentandra bark, Blumea balsamifera leaf and Artocarpus altilis leaf. Natural antioxidants from plant species are considered safe. Therefore, people are now more interest in finding out the treatment through natural remedies. The plant's total phenolic content was determined by the Folin-Ciocalteu method, while total flavonoid content was determined by the Colorimetric assay method. Antioxidant activity was evaluated by DPPH radical scavenging assay. The results showed that plant samples tested contained 22.02 to 214.88 µg of gallic acid equivalents (GAE)/mg extract represented the total phenolic content. The flavonoid content of the samples was in range from 50.00 to 896.67 µg of catechin equivalents (CE)/mg extract. The plant samples displayed ability to inhibit DPPH free radical formation by 59-81% at 50 ppm concentration. These finding suggest that these plants could possess natural antioxidants and give a scientific basis to the traditional uses of the investigated plants.

KANDUNGAN ANTIOKSIDAN PADA JAMUR TIRAM PUTIH (Pleurotus ostreatus) Saat Egra; Irawan Wijaya Kusuma; Enos Tangke Arung
ULIN: Jurnal Hutan Tropis Vol 2, No 2 (2018)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Indonesia merupakan negara hutan tropis yang luas, maka tak heran negara ini menjadi negara megabiodiversitas. Jamur tiram merupakan salah satu hasil kekayaan alam Indonesia yang memiliki potensi sebagai tanaman kesehatan dan obat. penelitian ini bertujuan untuk memunculkan potensi aktivitas antioksidan pada jamur tiram yang banyak dibudidaya di Kalimantan Timur khususnya Samarinda. Sampel kami ambil dari rumah jamur borneo dan penelitian ini dilaksanakan di Laboratorium Kimia Hasil Hutan, Fakultas Kehutanan, Universitas Mulawarman. Metode ekstraksi bertingkat menggunakan n-heksan, etil asetat, dan etanol. Metode antioksidan yang digunakan adalah penangkal radikal bebas (DPPH) dengan masing-masing tiga kali pengulangan. Hasil penelitian ini menunjukkan bahwa aktivitas antioksidan tertinggi pada ekstrak air dengan nilai penghambatan 25% pada kosentrasi 100 ppm. Pada ekstrak heksan merupakan hambatan terkecil dengan nilai penghambatan 2,41% pada kosentrasi 25ppm.

The Toxicity and Antibacterial Properties of Calamus ornatus Bl. Rattan Fruit Heriad Daud Salusu; Ernita Obeth; Abdul Rasyid Zarta; Eva Nurmarini; Hamka Nurkaya; Irawan Wijaya Kusuma; Enos Tangke Arung
agriTECH Vol 39, No 4 (2019)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Calamus ornatus Bl., locally known as rotan mantang or rotan seuti is one of the commercial rattan species growing in the forests of East Borneo. Its fruit is edible, like the fruits of other species of rattan, and it has been used by the local people as traditional medicine to treat stomachache or toothache. Until recently, there has not been any study investigating the benefits of the fruit for medicinal or other purposes. This research aimed to discover the bio-activity potentials of Calamus ornatus Bl. fruits by identifying its toxicity level and antibacterial potentials. The parts of the fruit (pericarp, flesh, and seed) were extracted using ethanol as solvent to extract samples. The toxicity was analyzed by using the Brine Shrimp Lethality Test (BSLT) method while the antibacterial activity was tested using a well diffusion method. Low toxicity was found in the flesh, whereas the pericarp and the seed were non-toxic. Antibacterial activity against Escherichia coli was found in the pericarp and the seed, while antibacterial activity against Streptococcus mutans was found in the pericarp and the flesh.

SKRINING FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL KAYU BATANG MENTAWA (Artocarpus anisophyllus Miq.) Octariana Toding Pabita; Erwin Erwin; Irawan Wijaya Kusuma
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2019
Publisher : PROSIDING SEMINAR KIMIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penelitian mengenai uji fitokimia dan aktivitas antioksidan pada ekstrak batang Mentawa (A. anisophyllus Miq.) telah dilakukan. Penelitian ini bertujuan untuk mengetahui kandungan senyawa metabolit sekunder dan nilai aktivitas antioksidan terhadap radikal bebas DPPH (2,2-diphenyl-1-picrylhidrazil) pada ekstrak metanol dari batang mentawa. Hasil uji fitokimia menunjukkan bahwa ekstrak metanol pada batang mentawa (A. anisophyllus Miq.) mengandung golongan senyawa flavonoid, fenolik dan triterpenoid dan hasil uji aktivitas antioksidan menunjukkan nilai IC50 ekstrak metanol yaitu 34.58 ppm. Hasil pengujian dengan menggunakan metode DPPH menunjukkan bahwa ekstrak kasar dikategorikan sebagai antioksidan sangat kuat. Kata Kunci : Mentawa, fitokimia, antioksidan, dan DPPH

Phytochemical Screening and Antioxidant Activity of Selekop (Lepisanthes amoena) Fruit Heriad Daud Salusu; Farida Ariani; Ernita Obeth; Mark Rayment; Edy Budiarso; Irawan Wijaya Kusuma; Enos Tangke Arung
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.810

Selekop (Lepisanthes amoena (Hassk.) Leenh.) plant leaves are used by the Dayak tribe of East Kalimantan as traditional cosmetics. Selekop fruit is also edible, but not well known. This study was conducted to obtain the phytochemical content and antioxidant assay in flesh, seed and pericarp extracts from the fruit of Selekop. Phytochemical analysis was conducted on ethanol extract for identification of flavonoid, alkaloid, tannin, saponin, triterpenoid and steroid. The antioxidant activity was done by DPPH assay with ascorbic acid as positive control. The flesh contained flavonoid, saponin, and tannin; the seed contained flavonoid, alkaloid, saponin, triterpenoid, and tannin; and the pericarp contained flavonoid, alkaloid, saponin, triterpenoid, and tannin. Analysis of antioxidant activity revealed the following Inhibitory Concentration (IC50 values): 122.51 ppm of flesh, 63.30 ppm of seed, 53.21 ppm of pericarp and 3.06 ppm of ascorbic acid. Based on these results, the ethanol extract of the seed and the flesh had a phytochemical content and antioxidant activity which was better than the flesh extract from Selekop fuit.

Aktivitas Antioksidan dan Antibakteri Ekstrak Daun Pometia pinnata Harlinda Kuspradini; Whicliffe Fiernaleonardo Pasedan; Irawan Wijaya Kusuma
Jurnal Jamu Indonesia Vol. 1 No. 1 (2016): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Penelitian ini bertujuan menentukan aktivitas antioksidan dan antibakteri berbagai jenis ekstrak daun Pometia pinnata. Kegiatan yang dilakukan meliputi proses ekstraksi daun Pometia pinnata dari Kalimantan Timur dengan teknik maserasi bertingkat menggunakan 3 pelarut: n-heksana, etil asetat, dan etanol. Ekstrak tersebut kemudian dievaluasi aktivitas antioksidan dan antimikrobanya.Dari hasil penelitian menunjukkan bahwa ekstrak daun P. pinnata mengandung alkaloid, tannin, dan kumarin. Hasil pengujian antioksidan dengan metode peredaman radikal bebas DPPH, pada konsentrasi 100 ppm menunjukkan aktivitas antioksidan yang tinggi dengan nilai 89,23%, 89,23% dan 90,38% pada masing-masing ekstrak n-heksana, etil asetat dan etanol. Nilai antioksidan menunjukkan nilai yang hampir sama dengan antioksidan vitamin C sebesar 96 pada konsentrasi 25. Hasil pengujian antimikroba dengan metode pewarnaan 2, 3, 5-triphenyl tetrazolium chloride (TTC) menunjukkan bahwa ekstrak daun P. pinnata memiliki kemampuan yang cukup kuat dalam menghambat pertumbuhan bakteri Streptococcus mutans, Streptococcus sobrinus, Escherichia coli kecuali terhadap Propionibacterium acne.

Aktivitas Anti Mikroba Dari Ekstrak Bawang Tiwai Suroto HS; Irawan Wijaya Kusuma
Jurnal Riset Teknologi Industri Vol 3 No 5 Juni 2009
Publisher : Balai Riset dan Standardisasi Industri Samarinda

Bawang tiwai (Eleutherine americana L. Merr) has chemical compound such as carbohydrate, protein, fat, alkaloid, flavonoid, carbocylate acid, aldehyde keton, thanin and phenol. Bawang tiwai is used by the ethnic of Dayak in Eas Kalimantan for spicy and medicine. Information about this chemical compound should be studied further and one of the study is to know the characteristic and activity of bawang tiwai as antimicrobial activity. The extract of bawang tiwai using methanol solvent has characteristic of brown concentrated solid (gummy), non crystalin, non aromatic and the extract of bawang tiwai using the solvent of n-tiexene. dietil ether and elit acetate has a characteristic of yellow brownies solid, bit oil, and both extractives are dissolved in acetone and methanol. Repeated seperetion with hexen, etil acetate, benzene-acetone using a coloum chromatography resulted in old yellow to brown fraction and aromatic. The fraclions resulted by step one and two separation are able to resist the growth of salmonella thypi and Bacillus cereus.

Co-Authors Abdul Rasyid Zarta Abdul Rasyid Zarta Agriandini, Maulina Agus Sulistyo Budi Amirta, Rudianto Ananda, Bayu Susetya Andi Syahid Khalid Assegraff Anjani, Ryan Arief Heru Prianto Aziza, Aziza Azizatul Karimah Binti Khairunnisa Dewi Listyaningrum, Dyah Ayu Dikarulin, Sarah Azzara Dungani, Rudi Edy Budiarso Edy Budiarso Enih Rosamah Enos Tangke Arung Enos Tangke Arung, Enos Tangke Ernita Obeth Erwin Erwin Erwin Erwin Erwin Erwin Esti Handayani Hardi Eva Nurmarini Fahriya Puspita Sari Faizatul Falah Farida Ariani Farida Aryani Farida Fitriani Purba Gina Saptiani Hamka Nurkaya Harlinda Kuspradini Heriad Daud Salusu Heriad Daud Salusu Heriad Daud Salusu Isna Yuniar Wardhani Khairunnisa, Binti Komsanah Sukarti Mark Rayment Muhammad Adly Rahandi Lubis Muhammad Akmal Rizqullah Muhammad Wildan Rizqi Miftahussanadi Nataniel Tandirogang Nissa Nurfajrin Solihat Nugroho, Rudi Agung Nur Indriana Fitriah Nur Indriana Fitriah Nurkadina Nurkadina Octariana Toding Pabita Pasedan, Whicliffe Fiernaleonardo Putri, Tasya Ananda Rico Ramadhan, Rico Rikna Naila Salsabila Rita Rusman Rosamah, Enih Rosamah, Enih Rudianto Amirta Saat Egra Sari, Ayu Mitha Sari, Nur Maulida Sukemi Sukemi Suroto HS Suwinarti, Wiwin Whicliffe Fiernaleonardo Pasedan Wicaksono, Dian Anggoro Widya Fatriasari Wita Pradiani Yani, Sinar Yuliansyah Yuliansyah Yundari, Yundari Zuliani, Nur Endah

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