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All Journal Chemistry Indonesian Journal of Chemical Research Makara Journal of Science Mukhtabar: Journal of Medical Laboratory Technology Lontara Journal Of Health Science And Technology Hayyan Journal
Rasyid, Nur Qadri
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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Journal : Mukhtabar: Journal of Medical Laboratory Technology

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Effectiveness of Lime Concentration As A Modification of Turk's Reagent in Leucocyte Type Count Examination Arham, Chaerul; Rasyid, Nur Qadri; Rianto, Rifo; Moito, Rahma
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.695

Abstract

Background & Objective: Turk solution has a composition of glacial acetic acid, gentian violet, and distilled water. Lime (Citrus aurantifolia S) has a pH of 2.0 which is almost the same as acetic acid. Both components are weak acids that can lyse blood cells other than white blood cells. This study aims to determine the effectiveness of lime concentration as a modification of Turk's reagent in counting the number of leukocytes and knowing the concentration of 1%, 2%, or 3% which is more effective in counting the number of leukocytes. Method: This study aims to provide an overview of the effectiveness of lime concentration as a modification of turk reagent composition in counting the number of leukocytes. The research method used is the Counting Room Method. This research was conducted in August 2024 at the clinical pathology laboratory of the Muhammadiyah Makassar Polytechnic. The number of samples in this study was 4 blood samples obtained from 4 correspondents with treatment, lime concentrations namely 1%, 2%, and 3%, and turk reagent as control. Result: At 1% concentration, the average difference in leucocyte count between the control and the sample was 1,150 cells/mm3, at 2% concentration a difference of 2,788 cells/mm3 was obtained with the control, and at 3% concentration a difference of 3,638 cells/mm3 was obtained. Conclusion: It can be concluded that the concentration of lime that is effective as a modification of Turk's reagent in counting the number of leukocytes is a concentration of 1%.
Identification of Malassezia sp. as a Cause of Dandruff Among Students at Muhammadiyah Polytechnic Makassar Annisa, Riska; Rasyid, Nur Qadri; Basarang, Mujahidah; Faisal, Restu Andini
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.703

Abstract

Background & Objective: Dandruff is a scalp condition attributed to the fungus Malassezia sp. It manifests as fine to coarse scales that are grayish or white, resulting from the shedding of the stratum corneum layer on the scalp. Factors contributing to the onset of dandruff include the proliferation of Malassezia fungi, sebaceous gland activity, and individual predisposition. This study aimed to identify Malassezia sp. responsible for dandruff among female students at the Muhammadiyah Polytechnic of Makassar. Method: The research employed a laboratory observational design utilizing a purposive sampling technique. Dandruff samples were collected from 10 female students and cultured on SDA (Sabouraud Dextrose Agar) media, followed by microscopic examination of the resulting colonies. Result: The findings revealed that none of the 10 dandruff samples contained Malassezia sp.; instead, contamination by Aspergillus fumigatus was observed. Conclusion: Therefore, it can be concluded that Malassezia sp. was not identified as a causative agent of dandruff in female students at the Muhammadiyah Polytechnic of Makassar
Co-Authors Abdul Razak Arham, Chaerul Arisanti, Dewi Basarang, Mujahidah Faisal, Restu Andini Gustini Syahbirin Hasma , Hasma Ishak, A. Nur Afni LAKSMI AMBARSARI Moito, Rahma Muawanah Muawanah Muawanah Muh. Nasir Muh. Rifo Rianto Nurhidayat Nurhidayat Nurhidayat Nurwijayanti Permata, Indra Purwantiningsih Sugita Riska Annisa Rustiah, Wa Ode Sri Wahyuni Susi Susanti