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Isolasi dan Karakterisasi Amilase dari Kapang Alkalotoleran Asal Limbah Cair Tapioka Mubarik, Nisa Rachmania; Damayanti, Evi; Listyowati, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 1 (2003): February 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.396 KB) | DOI: 10.24002/biota.v8i1.2786

Abstract

A total of 6 fungi isolates of growing at pH 9 and 2 isolates on pH 10 with amylolytic indexes 0f 0.07-1.42 have been isolated from cassava starch liquid waste. Two isolates having the highest amylolytic index were identified as Aspergillus sydowii K10 (1.42) and Aspergillus versicolor L30 (1.4). Both A. sydowii K10 and A. versicolor L30 were described as alcalotolerant for being able to grow with range pH 5-10. The optimal -amylases production of A. sydowii K10 and A. versicolor L30 was obtained after 4 and 3 days of incubation at 300C. The optimum of -amylase activity from A. sydowii K10 was at 400C and 700C, and pH 6; while those from A. versicolor L30 was at 500C and pH 6 respectively. Both A. sydowii K10 and A. versicolor L30 could produce glucoamylase. The optimum of glucoamylase activity from A. sydowii K10 was at 400C and pH 5, while those from A. versicolor L30 was at 500C and pH 5 respectively.
Aktivitas Terbang Sebelum Migrasi dan Identifikasi Polen Pada Madu Apis dorsata di Kampar, Riau: Flight Activity Before Migration and Pollen Identification from Honey of Apis dorsata in Kampar, Riau Dyahastuti, Meis; Raffiudin, Rika; Widjaja, Mochammad Chandra; Afriani, Nita; Listyowati, Sri
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.34-41

Abstract

Ketersediaan sumber pakan yang rendah menjadi salah satu permasalahan yang dihadapi petani madu lebah A. dorsata di Kampar, Riau. Aktivitas lebah terdiri dari spatial learning atau pengenalan lingkungan, foraging atau mencari sumber pakan dan aktivitas migrasi. Penelitian bertujuan menganalisis aktivitas terbang lebah dan mengidentifikasi keanekaragaman polen yang ada di dalam madu. Pengamatan aktivitas terbang dilakukan dengan menghitung aktivitas keluar sarang, masuk sarang tanpa polen dan masuk sarang dengan polen selama 3 hari dengan 3 interval waktu. Polen didapatkan dari madu asal Kampar, Riau. Aktivitas lebah A. dorsata keluar sarang, masuk ke sarang tanpa polen dan masuk ke sarang dengan polen selama 3 hari paling tinggi berada pada pukul 11-12, yang merupakan bukan waktu terbang untuk mencari nektar dan polen pada umumnya. Aktivitas terbang meningkat seiring dengan meningkatnya suhu dan intensitas cahaya. Sebanyak 13 spesies tumbuhan yang menjadi sumber pakan A. dorsata terdiri atas habitus pohon, perdu, semak dan herba. Jumlah persentase polen paling banyak yang menjadi sumber pakan A. dorsata merupakan habitus pohon yaitu Pentace sp. (64%), Eucalyptus alba (22.9%) dan Elaeis guineensis (4%).
Exploration of Pilobolus sp. Fungi from Various Livestock Manures in Kedung Pacul Village, Klaten Mahardhika, Wahyu Aji; Akmaliyah, Rizna; Diana, Milsa Solva; Listyowati, Sri
Bioma : Berkala Ilmiah Biologi Vol. 26, No 1, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.55874

Abstract

Pilobolus is a coprophilous fungus belonging to the Zygomycota. Pilobolus sp. is called a coprophilous fungus because it can live in animal feces. The uniqueness of this fungus is that it can shoot its spores, so Pilobolus sp. is called a shotgun fungus. Pilobolus sp. exhibits a phototropism mechanism in which the sporangium shoots spores in the direction of light. Pilobolus has a unique living habitat, namely in the manure of herbivores such as cows, goats, sheep, etc. This habit of life may seem terrible to us, but fungi like Pilobolus sp. are very important in life because they are a type of decomposer, capable of breaking down organic matter from dead living things. So the purpose of this study was to observe the spores produced by the fungus Pilobolus sp.  (a fungus in animal waste). The method used was to cultivate the fungus Pilobolus sp. for seven days on various animal wastes placed in jam bottles. The results showed that the Pilobolus sp. fungus grew on various media of animal feces, such as horses, cows, goats, and pigs, with different growth times, and there were also spores shot on the glass walls that had been perforated.