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All Journal Indonesian Journal of Cancer Chemoprevention Biomedika Medika Kartika : Jurnal Kedokteran dan Kesehatan Cerdika: Jurnal Ilmiah Indonesia
Nurpratami, Diah
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Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Health Professions Medicine & Pharmacology Public Health Social Sciences Veterinary

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PENGARUH WAKTU SIMPAN TERHADAP PERUBAHAN PH, GLUKOSA, LDH, KALSIUM, MVP SEBAGAI INDIKATOR KUALITAS KOMPONEN DARAH THROMBOCYTE CONCENTRATE Mentari, Diani; Pebrina, Relita; Nurpratami, Diah
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.8981

Abstract

ABSTRAK                Thrombocyte Concentrate (TC) merupakan komponen darah yang ditranfusikan untuk pasien yang mengalami pendarahan, kelainan fungsi trombosit dan trombositopenia. Pada beberapa literatur menyebutkan bahwa sediaan TC secara in vitro dapat disimpan selama 5-7 hari. Quality Control sebelum TC ditransfusikan yaitu melalui pengamatan secara visual ada tidaknya swirling dan melihat tingkat kejernihan TC. Namun pengamatan bersifat subjektif sehingga belum terstandar. Penelitian ini bertujuan untuk melihat pengaruh waktu penyimpanan TC terhadap kualitasnya. Kualitas TC diukur melalui beberapa cara yaitu pengukuran pH, kadar glukosa, Laktat Dehidrogenase (LDH), kalsium serta profil darah yang meliputi jumlah trombosit dan Mean Platelet Volume (MPV). Analisis kadar glukosa, LDH, dan kalsium dilakukan secara kolorimetri menggunakan spektrofotomter sedangkan profil darah diukur menggunakan hematology analyzer. Hasil menunjukkan bahwa TC yang disimpan pada hari ke-9 terjadi penurunan pH sebesar 7,38%, glukosa 20,10%, LDH 42,89%dan kalsium 62,54%. Jumlah trombosit mengalami penurunan sebesar 24,41% dan MVP mengalami kenaikan 18,84%. Kesimpulan pada sampel TC yang masih terdapat swirling, namun terjadi penurunan kualitas TC yang ditandai dengan penurunan jumlah trombosit serta kenaikan nilai MVP. Selain itu semakin lama waktu penyimpanan akan menurunkan kadar pH, kadar glukosa, LDH, dan kalsium. Kata Kunci: Masa Simpan Trombosit, pH, Glukosa, Kalsium, MPV. ABSTRACT                Thrombocyte Concentrate (TC) is the blood component tranfused for the patients with bleeding, abnormalities of platelet function and thrombocytopenia. In some literatures, it is mentioned that the TC preparation in vitro can be stored within 5-7 days.  Quality Control before TC is transfused through a visual observation on the presence or absence of swirling and the clarity level of TC. However, this observation is subjective and makes it unstandardized. This research aims to see the effects of the shelf life of thrombocytes on its quality. The TC quality was measured through a number of ways including: pH measurement, glucose level, Lactate Dehydrogenase (LDH), calcium and profiles of blood including number of thrombocytes, and Mean Platelet Volume (MPV). The analysis on the glucose level, LDH, and calcium was conducted by colorimetrical manner using the spectrophotometer and blood profile was measured using hematology analyzer. The result showed that TC stored in day 9 experienced the decrease of pH at 7.38%, glucose at 20.10%, LDH at 42,89% and calcium at 62.54%. The number of thrombocytes experienced a decrease of 24.41% and MVP experienced an increase of 18.84%. In conclusion, the sample of TC that had swirling experienced a decrease in the TC quality as characterized with the decrease of number of thrombocytes and the increase of MVP value. In addition, the longer storage could decrease the level of pH, level of glucose, LDH, and calcium. Keywords: Platelet Storage, pH, Glucose, Calcium, MPV.
EVALUASI KUALITAS THROMBOCYTE CONCENTRATE SELAMA MASA SIMPAN LIMA HARI BERDASARKAN VARIASI USIA PENDONOR Bria, Theodosia Claranty Nahak; Mentari, Diani; Sepvianti, Wiwit; Pebriani, Relita; Tirtana, Arif; Nurpratami, Diah
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 7 No 2 (2024): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Abstract

Thrombocyte Concentrate (TC) merupakan komponen darah yang digunakan untuk meningkatkan jumlah trombosit pada pasien yang mengalami trombositopenia. Komponen darah TC secara in vitro dapat disimpan selama 5 hari pada platelet agitator. Kontrol kualitas merupakan faktor yang penting untuk menjaga produk TC tetap dalam kondisi baik. Selama penyimpanan TC dapat mengalami berbagai perubahan kualitas. Usia merupakan faktor yang dapat memengaruhi kualitas darah itu sendiri. Semakin bertambahnya usia seseorang maka metabolisme tubuh akan ikut menurun. Hal ini dapat berpengaruh terhadap produksi trombosit. Saat ini, penelitian evaluasi kualitas TC pendonor dengan perbedaan usia belum pernah diteliti. Sampel TC diambil dari pendonor laki-laki berusia ≤ 30 dan > 30 tahun yang telah lolos seleksi donor, selanjutnya TC disimpan selama 5 hari. Sampel dianalisis pada hari ke 1, 3, 5 dengan beberapa parameter yaitu jumlah trombosit yang diukur menggunakan hemotology analyzer, serta swirling dan tingkat kekeruhan yang diukur secara visual. Pengamatan TC secara visual pada hari ke-5 menunjukkan swirling skor 2 dan nampak terjadi kekeruhan pada sampel TC pendonor ≤ 30 tahun. Selain itu jumlah TC yaitu 9 x 109 sel/unit. Pada pendonor usia > 30 tahun jauh lebih baik, dengan swirling skor 3 dan tidak adanya kekeruhan dan jumlah TC yaitu 26 x 109 sel/unit. Sampel darah TC dari pedonor usia > 30 tahun memiliki kualitas yang jauh lebih baik dibandingkan dengan pendonor ≤ 30 tahun. Maka dapat disimpulkan bahwa usia seseorang tidak dapat menentukan kualitas komponen darah TC. Kata kunci: kontrol kualitas, swirling, thrombocyte concentrate, trombosit, usiaDOI : 10.35990/mk.v7n2.p112-123
Human Platelet Lysate (HPL) as an Alternative Media Propagation of T47D Cells Line Mentari, Diani; Pebrina, Relita; Nurpratami, Diah
Indonesian Journal of Cancer Chemoprevention Vol 11, No 1 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss1pp36-45

Abstract

Fetal bovine serum (FBS) is a gold standard as a supplement to cell and tissue culture media. This is due to a large number of Growth Factor (GF) contained in FBS. However, the use of FBS is at risk of transferring endotoxins, prions, bacteria and viruses from animals to humans, so it is risky to be used on cell therapy. Human Platelet Lysate (HPL) is a medium that can be developed as an alternative cell growth medium. The advantage of HPL is that it does not contain aggregate platelets so it does not cause the cells to clot. This condition causes HPL to be used as a substitute medium replacing FBS for cell propagation. The use of HPL for cell propagation has been widely reported. However, the use of HPL in cancer cells has not been found. Thus, this study aims to see the effectiveness of HPL as a T47D cell culture medium. The study began with donor selection with criteria for the male sex, the blood type O, the age ≤35 years. Furthermore, the Platelet Concentrate (PC) was processed into HPL then measured pH, total protein and albumin levels. The cell viability was measured using the MTT assay to determine the ability of cell proliferation when propagation using HPL. The doubling time test was carried out as in the cell proliferation test. However, the incubation was carried out for 24 h, 48 h and 72 h and the HPL concentration used was 5%. The result shows that HPL 10% and 20% ability to increase proliferation better than the FBS 10%. HPL with a 5% concentration ability to shortens the doubling time than FBS 10% (doubling time is less than 19.94 h). It this study, cell proliferation is influenced by the pH of HPL and total protein but not by the amount albumin.Keywords: Human Platelet Lysate, Proliferation, T47D cell line, total protein, albumin.
Impact of Donor Age on Human Platelet Lysate Quality and its Consequential Effects on HeLa Cell Growth in the Presence of Anti-Cancer Compounds Mentari, Diani; Wijayanti, Gratiana Ekaningsih; Suhesti, Tuti Sri; Muhammad, Katon; Ritchie, Ni Ken; Nurpratami, Diah
Indonesian Journal of Cancer Chemoprevention Vol 14, No 3 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss3pp189-198

Abstract

An integral aspect of anticancer experimentation involves delineating the optimal dosage of the test compound to ascertain its efficacy in targeting malignant cells. Numerous variables may influence a compound's cytotoxicity, among which is the choice of cell culture medium. Within in vitro settings, supplementary mediums are employed to foster cellular proliferation. Platelet lysate (PL) serves as a growth supplement, presenting an alternative to fetal bovine serum (FBS), primarily due to its incorporation of growth factors such as platelet-derived growth factor (PDGF), a component absents in FBS. The integrity of PL may be subject to various factors, including the age of the donor. This study sought to evaluate the impact of donor age on PL quality. Furthermore, it aimed to discern whether PL derived from platelet concentrate (PC) blood components of different age cohorts influences the IC50 value in anticancer compound assessment. Expired PCs were utilized, subsequently classified into age categories: ≤30 years, >30 years, and a combination of ages. PL analysis encompassed parameters such as pH, blood profile, protein, glucose, and cholesterol levels. The investigation scrutinized the influence of PL quality, as a cellular growth supplement, on the anticancer compound cisplatin's activity against HeLa cells. Findings indicate that donor age influenced the IC50 value of cisplatin on HeLa cells. Notably, elevated cholesterol levels and decreased pH in PL from donor ages >30 years were associated with reduced cisplatin toxicity.Keywords: Cisplatin, Donor Age, HeLa, IC50, Platelet Lysate.
The Difference In Potassium Levels In Fresh Blood Bags With Blood Bags Stored At Pku Muhammadiyah Gamping Hospital Suryanto, Suryanto; Muhammad Gugun, Adang; Nurpratami, Diah
Cerdika: Jurnal Ilmiah Indonesia Vol. 4 No. 2 (2024): Cerdika : Jurnal Ilmiah Indonesia
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/cerdika.v4i2.758

Abstract

Blood transfusion, one of them, is a process of transferring blood components containing glucose, lactate, and potassium. During storage, blood cells undergo metabolic changes such as decreased blood pH, hemolysis, and increased potassium levels. One of the complications of transfusion that must be avoided is hyperkalemia due to the release of potassium. This study aimed to determine differences in stored blood potassium levels and PRC QC results during 11 days of storage. This was an analytic observational study with a cross sectional approach. A T-test was used to analyze the difference. In addition, PRC blood quality control (QC) was also carried out for stored blood according to standards. This study used 40 samples of fresh blood bags obtained from UTD PMI Yogyakarta City. The mean potassium level in fresh blood bags was 4.373 ± 0.417 mmol/L (3.70-5.50) and the average potassium level in blood bags stored for 6 days was 8.290 ± 0.275 mmol/L (7.80-8.90). There was a significant difference in potassium levels between fresh blood bags and blood bags stored for 6 days (p = 0.000). The overall QC PRC result is 50% which indicates the need for improvement in terms of the blood component separation process.
Co-Authors Bria, Theodosia Claranty Nahak Diani Mentari Muhammad Gugun, Adang Muhammad, Katon Ni Ken Ritchie, Ni Ken Pebriani, Relita Pebrina, Relita Sepvianti, Wiwit Suryanto Suryanto Tirtana, Arif Tuti Sri Suhesti Wijayanti, Gratiana Ekaningsih