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All Journal Indonesian Journal of Cancer Chemoprevention AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Indonesian Journal of Cancer Makara Journal of Health Research Media Penelitian dan Pengembangan Kesehatan
Ni Ken Ritchie, Ni Ken
Unit Donor Darah PMI DKI Jakarta Jl. Kramat Raya, Jakarta Pusat, Indonesia
Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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INKOMPATIBILITAS ABO PADA NEONATUS DI UTD PMI KOTA BANDA ACEH TAHUN 2018 Akbar, Teuku Ilhami Surya; Ritchie, Ni Ken; Sari, Nurmala
AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Averrous, Vol. 5: No. 2 (November, 2019)
Publisher : Fakultas Kedokteran Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.612 KB) | DOI: 10.29103/averrous.v5i2.2081

Abstract

Inkompatibilitas ABO atau ketidak cocokan golongan darah ABO pada neonatus merupakan salah satu penyebab ikterik patologis atau hiperbilirubinemia. Inkompatibilitas golongan darah ABO umumnya penyakit yang tidak berat, namun perlu penanganan sebaik-baiknya karena jika tidak dapat berdapak buruk bayi neonatus. Inkomptabilitas ABO terjadi pada 12% kehamilan, tetapi hanya 2% yang berkaitan dengan hemolisis berat. Ibu biasanya memiliki golongan darah O dan janin memiliki golongan darah A, B atau AB. Penelitian ini melihat prevalensi ABO inkompatibilitas pada neonatus  dan penanganan yang dilakukan di UTD PMI Kota Banda Aceh. Tujuan penelitian ini untuk mengetahui presentase inkompatibilitas ABO di UTD PMI Kota Banda Aceh dan juga penanganan yang selama ini dilakukan. Metoda penelitian ini menggunakan desain deskriptif dengan pendekatan cross sectional dan pengambilan data menggunakan data sekunder.  Total sampel berjumlah  4.500 kasus. Hasil penelitian menunjukkan presentase Inkompatibilitas ABO pada neonatus sebesar 0,5% dan diberikan penanganan berupa fototerapi dan transfusi darah. Kesimpulannya 1/20 neonatus yang diperiksakan darahnya di UTD PMI Kota Banda Aceh mengalami inkompatibilitas ABO, diharapkan ibu hamil dapat mengetahui golongan darahnya sebelum persalinan dan melakukan pemeriksaan terkait resiko inkompatibilitas.
Stimulation Effect of Exosome From Healthy Sera to Natural Killer (NK) Cells of Hepatocellular Carcinoma Subject In Vitro Deby, Deby; Antarianto, Radiana Dhewayani; Barasila, Atikah Chalida; Irawan, Cosphiadi; Ahani, Ardhi Rahman; Jasirwan, Chyntia Olivia Maurine; Damayanti, Lia; Ritchie, Ni Ken; Aditya, Robby Nur
Indonesian Journal of Cancer Vol 18, No 4 (2024): December
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v18i4.1122

Abstract

Background: Hepatocellular carcinoma has a poor prognosis due to limitations of therapy such as late diagnosis, lack of specific biomarkers, and insensitivity to this tumor agent. This study aims to develop immunotherapy using autologous natural killer cells (NK cells) with exosome stimulation for hepatocellular carcinoma patients, addressing treatment limitations.Methods: Experimental research conducted from October 2022 to June 2023 at Universitas Indonesia’s Faculty of Medicine involved three hepatocellular carcinoma patients at Dr. Cipto Mangunkusumo General Hospital in Jakarta, Indonesia. NK cells from hepatocellular carcinoma patients were isolated from peripheral venous blood, and exosomes were isolated from the blood serum of healthy donors. Exosome characterization with a particle size analyzer and flow cytometry. Stimulation of exosomes on NK cells for 24 hours, then evaluation of expression of NKp44, NKp46, NKp30, NKG2D, KIR2D, and NKG2A receptors, as well as perforin and granzyme B expression. Visualization of interactions of NK cells with other mononuclear cell fractions (CD4, CD8, CD11c, and CD19) by immunofluorescence. The study compares stimulated and unstimulated NK cells, analyzing their expression of activated and inhibitory receptors, using either the One-Way Anova parametric test or the Kruskal-Wallis non-parametric test for non-normally distributed data.Results: Particle size 100 nm, negative electric charge, and CD63+CD81+ (double positive) exosome isolated results. There was increased expression of receptors NKp44, NKp46, NKp30, NKG2D, decreased expression of NKG2A, and increased expression of perforin and granzyme B in exosome-induced NK cells. There was no cell interaction in the form of immune synapses between exosome-induced NK cells and other mononuclear cell fractions in hepatocellular carcinoma patients. Conclusions: Induction of exosomes into NK cells of hepatocellular carcinoma patients restores the cytotoxic ability of NK cells
Histodynamics of Natural Killer Cells from a Healthy Donor Exposed to Exosomes from the Blood of Hepatocellular Carcinoma Patients Asrinda, Indria; Antarianto, Radiana Dhewayani; Jusuf, Ahmad Aulia; Ahani, Ardhi Rahman; Jasirwan, Chyntia Olivia Maurine; Ritchie, Ni Ken; Nur Aditya, Robby; Irawan, Cosphiadi
Makara Journal of Health Research Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

Background: Hepatocellular carcinoma (HCC) is the leading form of liver cancer and the second leading cause of cancer-related deaths globally. Exosomes in the HCC microenvironment can induce significant changes in natural killer (NK) cells during endocytosis. The present study aimed to distinguish exosomes in the blood of HCC patients, analyze changes in NK cell phenotype, and evaluate peroxidase and toluidine blue staining as alternative methods for observing the changes. Methods: NK cells were collected from healthy donors, and exosomes were extracted from the blood of HCC patients. The exosomes were characterized in accordance with MISEV 2018 guidelines, and NK cells were incubated with HCC-derived exosomes. NK cell phenotype changes were assessed using immunofluorescence, toluidine blue staining, and peroxidase staining. Results: The identified exosomes measured 34.7 nm, had a charge of −4.33 mV, and were positive for CD81+. Changes in NK cell receptor expression following exposure to HCC exosomes were not significant (p > 0.05). Immunofluorescence confirmed exosome endocytosis by NK cells, toluidine blue staining revealed negative metachromasia and peroxidase staining indicated morphological NK cell changes. Conclusions: This study demonstrates that peroxidase and toluidine blue staining are effective for observing exosome endocytosis in NK cells, enhancing our understanding of HCC exosome-NK cell interactions and beneficial in developing future therapeutic strategies targeting the HCC microenvironment.
EFFECT OF MEAL TIME BEFORE BLOOD DONATION ON PLASMA LIPEMIC LEVELS AND IN LIQUID PLASMA (LP) Anzhari, Della Hashfi; Ritchie, Ni Ken
Media Penelitian dan Pengembangan Kesehatan Vol. 35 No. 3 (2025): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v35i3.3121

Abstract

Kondisi meningkatnya kadar lemak dalam darah akibat asupan makanan dapat mengindikasikan hiperlipidemia. Peningkatan kadar lemak dalam darah pasca makan merupakan respons fisiologis normal terhadap asupan lemak dari makanan. Lemak di dalam tubuh akan mengalami metabolisme dan membutuhkan waktu kembali ke keadaan basal. Hal ini dapat menyebabkan lipemik yang disebabkan oleh tingginya lipoprotein yang terdapat pada darah. Penelitian ini bertujuan untuk mengevaluasi pengaruh waktu makan sebelum donasi terhadap kadar trigliserida dan kejadian lipemik pada komponen LP. Metode studi eksperimen dilakukan pada 28 responden yang dibagi menjadi dua kelompok berdasarkan waktu makan terakhir (2 dan 3 jam sebelum donor). Darah donor yang terkumpul dibuat menjadi komponen darah Liquid Plasma (LP) dan Packed Red Cell (PRC) leukodepleted dilakukan pemeriksaan lipemik, kadar kolesterol,dan kadar trigliserida. Hasil penelitian menunjukkan tidak ditemukan pengaruh waktu makan 2 jam dan 3 jam terhadap kenaikan kadar kolesterol sebelum donor dengan darah donor maupun komponen LP (p=0,946, P=0,431). Ditemukan pengaruh waktu makan 2 jam dan 3 jam terhadap kenaikan kadar trigliserida sebelum donor dengan darah donor maupun komponen LP (p=0,002, p=0,003) dimana berdasarkan nilai mean selisih kenaikan cenderung lebih tinggi pada waktu makan 3 jam sebelum donor. Terdapat hubungan antara kadar trigliserida dan lipemik (p=0,000). Namun, tidak terdapat hubungan antara kadar kolesterol dengan lipemik yang terjadi pada komponen LP (p=0,229).
Impact of Donor Age on Human Platelet Lysate Quality and its Consequential Effects on HeLa Cell Growth in the Presence of Anti-Cancer Compounds Mentari, Diani; Wijayanti, Gratiana Ekaningsih; Suhesti, Tuti Sri; Muhammad, Katon; Ritchie, Ni Ken; Nurpratami, Diah
Indonesian Journal of Cancer Chemoprevention Vol 14, No 3 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss3pp189-198

Abstract

An integral aspect of anticancer experimentation involves delineating the optimal dosage of the test compound to ascertain its efficacy in targeting malignant cells. Numerous variables may influence a compound's cytotoxicity, among which is the choice of cell culture medium. Within in vitro settings, supplementary mediums are employed to foster cellular proliferation. Platelet lysate (PL) serves as a growth supplement, presenting an alternative to fetal bovine serum (FBS), primarily due to its incorporation of growth factors such as platelet-derived growth factor (PDGF), a component absents in FBS. The integrity of PL may be subject to various factors, including the age of the donor. This study sought to evaluate the impact of donor age on PL quality. Furthermore, it aimed to discern whether PL derived from platelet concentrate (PC) blood components of different age cohorts influences the IC50 value in anticancer compound assessment. Expired PCs were utilized, subsequently classified into age categories: ≤30 years, >30 years, and a combination of ages. PL analysis encompassed parameters such as pH, blood profile, protein, glucose, and cholesterol levels. The investigation scrutinized the influence of PL quality, as a cellular growth supplement, on the anticancer compound cisplatin's activity against HeLa cells. Findings indicate that donor age influenced the IC50 value of cisplatin on HeLa cells. Notably, elevated cholesterol levels and decreased pH in PL from donor ages >30 years were associated with reduced cisplatin toxicity.Keywords: Cisplatin, Donor Age, HeLa, IC50, Platelet Lysate.
DONOR DARAH DENGAN HIPERLIPIDEMIA BERDAMPAK TERHADAP KUALITAS DARAH YANG DISUMBANGKAN: SYSTEMATIC REVIEW Anzhari, Della Hashfi; Ritchie, Ni Ken; Lubis, Anna Mira
Media Penelitian dan Pengembangan Kesehatan Vol. 34 No. 4 (2024): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v34i4.2647

Abstract

Hyperlipidemia is currently suffered by many people, including those individuals who regularly donate blood. Hyperlipidemia occurs due to an unhealthy lifestyle. It was found that there was a decrease in the quality of donor blood, including a change in plasma color to a milky white, cloudy appearance known as lipemia, as well as a decrease in the function of platelet and erythrocyte components. The aim of this study was to determine the impact of hyperlipidemia in blood donors on the blood produced. This systematic literature review follows PRISMA guidelines, with article collection carried out using basic data from Science Direct, PubMed, Google Scholar, Research Gate and Sage Journal. The articles used were limited to publication years between 2014 and 2024. In this research, 7 articles were found that were suitable and selected. The results of this study show that there are several factors that cause an individual to experience hyperlipidemia, one of the factors that has a big influence is diet. In this study, it was discovered that donors with hyperlipidemia were more likely to produce blood products that had high levels of fat, in which case the plasma blood component became lipemic and level of hemolysis during storage was higher. Apart from that, hyperlipidemia also affects other blood products, namely the platelet component, which can produce platelets that have abnormalities in platelet composition and function. The impact of hyperlipidemia should not be ignored, better donor screening can provide better quality control to reduce the risk of this occurring.
Co-Authors Aditya, Robby Nur Ahani, Ardhi Rahman Ahmad Aulia Anna Mira Lubis Antarianto, Radiana Dhewayani Anzhari, Della Hashfi Asrinda, Indria Barasila, Atikah Chalida Chyntia Olivia Maurine Jasirwan, Chyntia Olivia Maurine Cosphiadi Irawan Deby Deby Diani Mentari Lia Damayanti Muhammad, Katon Nur Aditya, Robby Nurmala Sari Nurpratami, Diah Teuku Ilhami Surya Akbar Tuti Sri Suhesti Wijayanti, Gratiana Ekaningsih