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All Journal AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Indonesian Journal of Cancer Makara Journal of Health Research Media Penelitian dan Pengembangan Kesehatan
Ni Ken Ritchie, Ni Ken
Unit Donor Darah PMI DKI Jakarta Jl. Kramat Raya, Jakarta Pusat, Indonesia
Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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INKOMPATIBILITAS ABO PADA NEONATUS DI UTD PMI KOTA BANDA ACEH TAHUN 2018 Akbar, Teuku Ilhami Surya; Ritchie, Ni Ken; Sari, Nurmala
AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Averrous, Vol. 5: No. 2 (November, 2019)
Publisher : Fakultas Kedokteran Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.612 KB) | DOI: 10.29103/averrous.v5i2.2081

Abstract

Inkompatibilitas ABO atau ketidak cocokan golongan darah ABO pada neonatus merupakan salah satu penyebab ikterik patologis atau hiperbilirubinemia. Inkompatibilitas golongan darah ABO umumnya penyakit yang tidak berat, namun perlu penanganan sebaik-baiknya karena jika tidak dapat berdapak buruk bayi neonatus. Inkomptabilitas ABO terjadi pada 12% kehamilan, tetapi hanya 2% yang berkaitan dengan hemolisis berat. Ibu biasanya memiliki golongan darah O dan janin memiliki golongan darah A, B atau AB. Penelitian ini melihat prevalensi ABO inkompatibilitas pada neonatus  dan penanganan yang dilakukan di UTD PMI Kota Banda Aceh. Tujuan penelitian ini untuk mengetahui presentase inkompatibilitas ABO di UTD PMI Kota Banda Aceh dan juga penanganan yang selama ini dilakukan. Metoda penelitian ini menggunakan desain deskriptif dengan pendekatan cross sectional dan pengambilan data menggunakan data sekunder.  Total sampel berjumlah  4.500 kasus. Hasil penelitian menunjukkan presentase Inkompatibilitas ABO pada neonatus sebesar 0,5% dan diberikan penanganan berupa fototerapi dan transfusi darah. Kesimpulannya 1/20 neonatus yang diperiksakan darahnya di UTD PMI Kota Banda Aceh mengalami inkompatibilitas ABO, diharapkan ibu hamil dapat mengetahui golongan darahnya sebelum persalinan dan melakukan pemeriksaan terkait resiko inkompatibilitas.
Stimulation Effect of Exosome From Healthy Sera to Natural Killer (NK) Cells of Hepatocellular Carcinoma Subject In Vitro Deby, Deby; Antarianto, Radiana Dhewayani; Barasila, Atikah Chalida; Irawan, Cosphiadi; Ahani, Ardhi Rahman; Jasirwan, Chyntia Olivia Maurine; Damayanti, Lia; Ritchie, Ni Ken; Aditya, Robby Nur
Indonesian Journal of Cancer Vol 18, No 4 (2024): December
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v18i4.1122

Abstract

Background: Hepatocellular carcinoma has a poor prognosis due to limitations of therapy such as late diagnosis, lack of specific biomarkers, and insensitivity to this tumor agent. This study aims to develop immunotherapy using autologous natural killer cells (NK cells) with exosome stimulation for hepatocellular carcinoma patients, addressing treatment limitations.Methods: Experimental research conducted from October 2022 to June 2023 at Universitas Indonesia’s Faculty of Medicine involved three hepatocellular carcinoma patients at Dr. Cipto Mangunkusumo General Hospital in Jakarta, Indonesia. NK cells from hepatocellular carcinoma patients were isolated from peripheral venous blood, and exosomes were isolated from the blood serum of healthy donors. Exosome characterization with a particle size analyzer and flow cytometry. Stimulation of exosomes on NK cells for 24 hours, then evaluation of expression of NKp44, NKp46, NKp30, NKG2D, KIR2D, and NKG2A receptors, as well as perforin and granzyme B expression. Visualization of interactions of NK cells with other mononuclear cell fractions (CD4, CD8, CD11c, and CD19) by immunofluorescence. The study compares stimulated and unstimulated NK cells, analyzing their expression of activated and inhibitory receptors, using either the One-Way Anova parametric test or the Kruskal-Wallis non-parametric test for non-normally distributed data.Results: Particle size 100 nm, negative electric charge, and CD63+CD81+ (double positive) exosome isolated results. There was increased expression of receptors NKp44, NKp46, NKp30, NKG2D, decreased expression of NKG2A, and increased expression of perforin and granzyme B in exosome-induced NK cells. There was no cell interaction in the form of immune synapses between exosome-induced NK cells and other mononuclear cell fractions in hepatocellular carcinoma patients. Conclusions: Induction of exosomes into NK cells of hepatocellular carcinoma patients restores the cytotoxic ability of NK cells
Histodynamics of Natural Killer Cells from a Healthy Donor Exposed to Exosomes from the Blood of Hepatocellular Carcinoma Patients Asrinda, Indria; Antarianto, Radiana Dhewayani; Jusuf, Ahmad Aulia; Ahani, Ardhi Rahman; Jasirwan, Chyntia Olivia Maurine; Ritchie, Ni Ken; Nur Aditya, Robby; Irawan, Cosphiadi
Makara Journal of Health Research
Publisher : UI Scholars Hub

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Abstract

Background: Hepatocellular carcinoma (HCC) is the leading form of liver cancer and the second leading cause of cancer-related deaths globally. Exosomes in the HCC microenvironment can induce significant changes in natural killer (NK) cells during endocytosis. The present study aimed to distinguish exosomes in the blood of HCC patients, analyze changes in NK cell phenotype, and evaluate peroxidase and toluidine blue staining as alternative methods for observing the changes. Methods: NK cells were collected from healthy donors, and exosomes were extracted from the blood of HCC patients. The exosomes were characterized in accordance with MISEV 2018 guidelines, and NK cells were incubated with HCC-derived exosomes. NK cell phenotype changes were assessed using immunofluorescence, toluidine blue staining, and peroxidase staining. Results: The identified exosomes measured 34.7 nm, had a charge of −4.33 mV, and were positive for CD81+. Changes in NK cell receptor expression following exposure to HCC exosomes were not significant (p > 0.05). Immunofluorescence confirmed exosome endocytosis by NK cells, toluidine blue staining revealed negative metachromasia and peroxidase staining indicated morphological NK cell changes. Conclusions: This study demonstrates that peroxidase and toluidine blue staining are effective for observing exosome endocytosis in NK cells, enhancing our understanding of HCC exosome-NK cell interactions and beneficial in developing future therapeutic strategies targeting the HCC microenvironment.
EFFECT OF MEAL TIME BEFORE BLOOD DONATION ON PLASMA LIPEMIC LEVELS AND IN LIQUID PLASMA (LP) Anzhari, Della Hashfi; Ritchie, Ni Ken
Media Penelitian dan Pengembangan Kesehatan Vol. 35 No. 3 (2025): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v35i3.3121

Abstract

Kondisi meningkatnya kadar lemak dalam darah akibat asupan makanan dapat mengindikasikan hiperlipidemia. Peningkatan kadar lemak dalam darah pasca makan merupakan respons fisiologis normal terhadap asupan lemak dari makanan. Lemak di dalam tubuh akan mengalami metabolisme dan membutuhkan waktu kembali ke keadaan basal. Hal ini dapat menyebabkan lipemik yang disebabkan oleh tingginya lipoprotein yang terdapat pada darah. Penelitian ini bertujuan untuk mengevaluasi pengaruh waktu makan sebelum donasi terhadap kadar trigliserida dan kejadian lipemik pada komponen LP. Metode studi eksperimen dilakukan pada 28 responden yang dibagi menjadi dua kelompok berdasarkan waktu makan terakhir (2 dan 3 jam sebelum donor). Darah donor yang terkumpul dibuat menjadi komponen darah Liquid Plasma (LP) dan Packed Red Cell (PRC) leukodepleted dilakukan pemeriksaan lipemik, kadar kolesterol,dan kadar trigliserida. Hasil penelitian menunjukkan tidak ditemukan pengaruh waktu makan 2 jam dan 3 jam terhadap kenaikan kadar kolesterol sebelum donor dengan darah donor maupun komponen LP (p=0,946, P=0,431). Ditemukan pengaruh waktu makan 2 jam dan 3 jam terhadap kenaikan kadar trigliserida sebelum donor dengan darah donor maupun komponen LP (p=0,002, p=0,003) dimana berdasarkan nilai mean selisih kenaikan cenderung lebih tinggi pada waktu makan 3 jam sebelum donor. Terdapat hubungan antara kadar trigliserida dan lipemik (p=0,000). Namun, tidak terdapat hubungan antara kadar kolesterol dengan lipemik yang terjadi pada komponen LP (p=0,229).
Co-Authors Aditya, Robby Nur Ahani, Ardhi Rahman Ahmad Aulia Antarianto, Radiana Dhewayani Anzhari, Della Hashfi Asrinda, Indria Barasila, Atikah Chalida Chyntia Olivia Maurine Jasirwan, Chyntia Olivia Maurine Cosphiadi Irawan Deby Deby Lia Damayanti Nur Aditya, Robby Nurmala Sari Teuku Ilhami Surya Akbar