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All Journal Jurnal Penelitian Pertanian Terapan Agrin : Jurnal Penelitian Pertanian Jurnal Penelitian IPTEKS PEKAT : Jurnal Pengabdian Kepada Masyarakat International Applied Science Callus: Journal of Agrotechnology Science Agrimas : Jurnal Pengabdian Masyarakat Bidang Pertanian
Murtiyaningsih, Hidayah
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Journal : International Applied Science

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Multiplication Of Pisang “Agung” (Musa Paradisiaca L.) With In Vitro As A Local Product For Food Security Fatechan, Billy; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.630

Abstract

Pisang “Agung”(Musa paradisiaca L.) is one of the banana varieties originating from Lumajang Regency. Pisang Agung is famous for its large fruit and sweet taste. The conventional way of propagating Pisang “Agung” takes a long time, produces few shoots, is not uniform and is not guaranteed to be disease-free, these obstacles can be overcome by using tissue culture. The design that will be used in this study is a Non-Factorial Completely Randomized Design (CRD) consisting of 100 ppm BAP (B) which consists of 4 levels, namely B0 (0 ppm), B1 (3 ppm), B2 (6 ppm), B3 (9 ppm). The parameters observed were the age of shoot emergence, shoot height, number of shoots. The results showed that the treatment of various concentrations of BAP gave a significant effect on all observation parameters with the best treatment B3 age of bud emergence (9.25 days), bud height is B3 (7.35 cm) and the number of buds is B0 (3.90 pieces)
Genetic Diversity Test of Various Javanese Local Sorghum Us-ing RAPD Molecular Markers Utami, Erisa Putri; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.633

Abstract

Sorghum is one of the cereal crops that has good growth adaptation and production on dry land so that it has the potential to be developed in Indonesia. Besides being able to produce in dry areas, sorghum has a higher protein content compared to corn and cracked rice, but lower than wheat. Local sorghum used in this study included local Jember, local Demak, local Nganjuk, and local Majalengka. The level of genetic diversity of sorghum in Indonesia is still relatively low. Therefore, it is necessary to test the genetic diversity of sorghum with plant breeding. To obtain genetic diversity, this research conducted identification using RAPD molecular markers with 9 primers, namely OPA- 01, OPA-02, OPA-03, OPA-04, OPA-05, OPA- 06, OPA-11, OPA 17, and OPA 18. Based on the results of RAPD, the results of DNA bands were obtained which were continued with the NTSYSpc program to produce a kinship tree between local sorghum plants. From the results of the phylogenetic tree, it is known that the local sorghum of Majalengka and Lamongan have a parallel kinship, which means that the sorghum is genetically the same. Meanwhile, the genetic diversity of local sorghum in Jember and Demak is far compared to Lamongan and Majalengka. Nganjuk local sorghum has a genetic diversity line position in the middle (Jember, Demak) and (Majalengka, Nganjuk).
Screening of Auxin-Producing Bacteria from Several Crops Rhizospheres Prasetyo, Vandi Putranto; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.634

Abstract

Plants have natural compounds that can stimulate plant growth and development called phytohormones. Phytohormones can be produced endogenously by plants themselves and are not optimal. Therefore it requires exogenous phytohormones that come from outside the plant. In some cases, non-pathogenic bacteria can promote plant growth and be used in agriculture as biofertilizers. Soil rhizospheres from crops are suspected that the soil in the root area (rhizosphere) contains bacteria that stimulate plant growth. Hormones that play a role in plant growth function in division, enlargement of sprout cells, and division of cells at the root growth point. This research aims to determine bacteria that potentially produce the hormone auxin. This research identifies bacterial isolates from microscopic and macroscopic characterization. Screening isolates of auxin-producing bacteria using colorimetric methods and spectrophotometric methods. The results of characterization and screening from 5 samples showed that the bacterial samples that had the potential to produce auxin were samples isolated from eggplant soil (e) with a concentration of 6.5 ppm. The auxin produced is auxin IBA (Indole-3-butyric acid). The IBA auxin content is high enough to be applied as a plant growth-promoting bacteria.
Efforts to Propagate Sorghum In Vitro to Support Local Food Aisyah, Siti; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.635

Abstract

indonesia is a country known for its abundant biodiversity. however, the majority of its people still make rice as a daily staple food, making the price of rice continue to increase until it is difficult to reach by low-income people. therefore, it is necessary to develop alternative local food to replace rice to avoid a food crisis. One of the alternative plants is sorghum. Because sorghum has many benefits besides as food as well as bioenergy. That way it is necessary to make efforts to develop sorghum, one of which is by means of embryogenic callus tissue culture techniques by adding a concentration of 2,4-D. This research was conducted in the Laboratory of Nutraseutical and Pharmaseutical Division of Center for Development of Advancd Science and Technologies (CDAST) UPA Waste Management and Integrated Laboratory, University of Muhammadiyah Jember by using Completely Randomized Design (CRD) with a single factor namely A0 (control), A1 1 ppm, A2 2ppm, A3 3ppm, A4 4 ppm which was repeated 3 times. The variables under observation are the callus's color, texture, percentage, and time of formation. According to the study's findings, the application of 4 ppm 2,4-D produced the best results when compared to other 2,4-D treatments. These treatments were able to produce callus at a rate of 4.11 his, 100% of the total number of callus, crumbly texture, and pale yellowish color.
Co-Authors Anggriawan, Rendy Arum, Laras Sekar Ega Dwi Sukmadiningsih Fatechan, Billy Fodesta, Fiana Hadianto, Mohamad Gozali Hasbi, Hudaini Hazmi, M. Hazmi Hendriyanto, Aldi Kumara Hadi, Danang Mega Saniya Juli Nuriyatul Muhammad Hazmi Muliasari, Risa Martha Nugroho, Tito Agung Oktarina Oktarina Prasetyo, Vandi Putranto Rahayu, Reni Puji Ramadhan, Moch. Alfian Rizky Restanto, Didik Puji Rima, Baidhatul Khoirimah Siti Aisyah Suroso, Bejo Utami, Erisa Putri