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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioeksperimen: Jurnal Penelitian Biologi Pharmascience PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Jejaring Matematika dan Sains Jurnal Farmasi dan Sains Indonesia (JFSI) Journal of Experimental and Clinical Pharmacy (JECP) Bioma : Jurnal Ilmiah Biologi NAWASENA: Journal of Community Service
Gunawan, Yohanes Edy
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

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The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3 Neneng, Liswara; Gunawan, Yohanes Edy
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.306 KB) | DOI: 10.11594/jtls.08.01.04

Abstract

Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia.  These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates.  This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory.  Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control.  All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3.  Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.
Linking Zoopharmacognocy with Ethnomedication, An Evidence Base from Sebangau National Park, Central Kalimantan Indonesia Panda, Adventus; Gunawan, Yohanes Edy
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.15

Abstract

The ability of animal to perform self-medication has been studied decades, as well as their relationship with medication practices by communities. Long-term observation of Orangutans' behaviour (Pongo pygmaeus wurmbii), by communities surround Sebangau National Park, has suggested as their modes to today ethno-medication practice. The study was aimed to study the relationship of Orangutans self-medicate behavior with ethno-medication practice, in Sebangau area. The research was conducted in Punggualas, SNP, from 29 April to 03 October 2017. A number of 13 traditional healers (TH) from Karuing (n = 4), Baun Bango (n = 4), and Jahanjang (n = 5) have been interviewed. All plants are listed and photograph. Plants parts, and their mechanism of utilization were also kept for record. Meanwhile, the behavior followed the Orangutan protocol, with focus on their feeding behavior. All data were analyzed descriptively, while the relationship was analyzed using chi-square and F test. We have recorded a total 131 plants at various life forms, in Baun Bango (n = 59), Jahanjang (n = 41), and Karuing (n = 21). Plants that were found similar among three villages, removed, were only 95 left. We observed one female, showing the ability to perform self-mediaction. It is characterized by selectively choosing young leaves of Mezzetia sp., pulp of the Dyera lowii and Ilex cymosa, and lastly chew the entire leaves of Belang Handipek. It suggests a form of prevention against fatigue conditions, and the combination of these three plants species assume to be relating to fitness. This study shows that there is a relationship between the plant part used by the traditional healer and the orang- utan (x2 = 43,887; n = 115, df = 11, p-value = 0.0000), the relationship between the use of plant parts utilized by the traditional healer and orangutans (x2 = 15, 647; n = 50, df = 8, p-value = 0.0000). Furthermore, there is a relationship between the practice of traditional healer treatment using plant parts and Orang-utan (F1, 113 = 230.158; p-value = 0,000). The study urges to isolate secondary metabolites for further investigation, especially in terms of phyto-pharmacy.
Identifikasi Jenis Kupu-Kupu (Lepidoptera) Di Universitas Palangka Raya Gonggoli, Ade Damara; Sari, Sartika; Oktofiani, Helen; Santika, Novira; Herlina, Rini; Agatha, Tiara; Gunawan, Yohanes Edy
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: Maret 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i1.10361

Abstract

Tujuan penelitian adalah untuk mengidentifikasi jenis kupu-kupu yang berada di Universitas Palangkaraya pada lahan yang setelah terbakar dan lahan yang belum terbakar. Metode penelitian menggunakan metode observasi lapangan dengan teknik net sweeping dan jelajah. Dari hasil identifikasi jenis kupu-kupu yang dilakukan di sekitar kawasan UPR menunjukkan bahwa jumlah total jenis kupu-kupu yang ditemukan sebanyak 10 spesies yang tergolong ke dalam empat famili masing-masing yaitu famili Nymphalidae (3 jenis), famili Pleridae (5 jenis), famili Papilionidae (1 jenis), dan Lycaenidae (1 jenis), dengan total jumlah individu sebanyak 30 ekor, yang di dominasi oleh spesies Acraea terpsicore.
Pengaruh Pemberian Air Rebusan Akar Ilalang (Imperata cylindrica) Terhadap Stamina Mencit Jantan (Mus musculus): The Influence of Ilalang Root Drinking Water (Imperata cylindrica) Against Stamina Chicking A Heart (Mus musculus) Sumarsono, Dito Dwi; Gunawan, Yohanes Edy; Panda, Adventus
Jurnal Jejaring Matematika dan Sains Vol. 1 No. 2 (2019): Edisi Desember 2019
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/jjms.v1i2.216

Abstract

Ilalang (Imperata cylindrica) termasuk famili Poaceae. Akar tumbuhan ini oleh sebagian masyarakat di Kalimantan Tengah digunakan untuk meningkatkan stamina. Masyarakat yang memanfaatkan air rebusan akar ilalang agar tidak mudah lelah. Tujuan penelitian ini untuk mengetahui khasiat air rebusan ilalang terhadap stamina mencit Swiss Webster jantan. Penelitian ini dilakukan dengan acar menghitung frekuensi tunggangan mencit jantan selama satu jam. Penelitian ini merupakan penelitian eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan masing-masing perlakuan memiliki 6 kali ulangan. Masing-masing perlakuan menggunakan akuades, minuman berenergi 13 mL/kg BB, Rebusan air akar ilalang 9 mL/kg BB, dan rebusan air akar ilalang 18 mL/kg BB. Analisis data menggunakan aplikasi spss PASW versi 18 dengan uji ANOVA 1 arah (one way anova) dan uji lanjut Duncan’s Multiple Range Test (DMRT) dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan air rebusan akar ilalang meningkatkan stamina mencit jantan secara nyata (Fhit 11,51 > Ftab 3,10). Hasil uji lanjut menggunakan DMRT menunjukkan pemberian air rebusan akar ilalang 18 mL/kg BB memiliki rata-rata frekuensi kawin tertinggi 47,50 kali dibandingkan dengan tiga kelompok perlakuan lainnya. Kesimpulan; air rebusan akar ilalang meningkatkan stamina mencit Swiss Webster jantan.
Gambaran Histopatologi Hati dan Ginjal Mencit (Mus musculus) Setelah Pemberian Ekstrak Daun Bajakah Kalalawit (Uncaria gambir Roxb.) Gunawan, Yohanes Edy; Priyadi, Muhammad; Citrariana, Shesanthi; Saputra, Rizki Rachmad; Decenly, Decenly; Fauziah, Anisa Rahma; Febrianto, Yahya; Suprayogi, Thathit
Jurnal Pharmascience Vol 11, No 2 (2024): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i2.17733

Abstract

Secara empiris Bajakah Kalalawit (Uncaria gambir Roxb.) telah digunakan oleh masyarakat lokal Kalimantan karena dipercaya dapat menyembuhkan berbagai macam penyakit. Beberapa penelitian telah menunjukkan potensi aktivitas farmakalogis bajakah kalalawit dimulai dari bagian batang, akar dan lainnya. Namun, apabila tidak digunakan dengan tepat maka berpotensi dapat membahayakan penggunanya. Tujuan dari penelitian ini adalah menganalisis aspek toksikologi konsumsi ekstrak air daun bajakah kalalawit melalui gambaran histopatologi organ hati dan ginjal. Ekstraksi daun bajakah kalalawit menggunakan metode refluks dengan pelarut air dan uji histopatologi menggunakan pengamatan mikroskopis histologi pada organ hati dan ginjal mencit jantan (Mus musculus) melalui pemberian dosis ekstrak air daun bajakah kalalawit sebanyak 52,5 mg/KgBB, 105 mg/KgBB, dan 210 mg/KgBB selama 30 hari. Hasil penelitian menunjukkan bahwa pemberian ekstrak air daun bajakah kalalawit selama 30 hari dapat menimbulkan terjadinya degenerasi sel dan nekrosis sel pada organ hati maupun ginjal mencit. Dapat disimpulkan bahwa pemberian ekstrak air daun bajakah kalalawit dengan dosis 52,5 mg/KgBB, 105 mg/KgBB dan 210 mg/KgBB dapat menyebabkan perubahan histologi pada organ hati dan ginjal. Kata Kunci: Histologi, Ekstrak, Toksikologi, Mikroskopis, Nekrosis   Empirically, Bajakah Kalalawit (Uncaria gambir Roxb.) has been used by local Kalimantan communities because it is believed to cure various diseases. Several studies have shown the potential pharmacalogical activity of bajakah kalalawit starting from the stem, root and other parts. However, if not used properly, it can potentially harm the user.. The purpose of this study was to determine the toxicology aspects of consumption of bajakah kalalawit leaf water extract through histopathological images of liver and kidney organs. Extraction of bajakah kalalawit leaves using reflux method with water solvent and histopathology test using microscopic observation of liver and kidney histology of male mice (Mus musculus) which were treated with doses of bajakah kalalawit leaf water extract 52.5 mg/KgBW, 105 mg/KgBW, and 210 mg/KgBW for 30 days.  The results showed that the administration of bajakah kalalawit leaf water extract for 30 days can cause cell degeneration and cell necrosis in the liver and kidney organs of mice. It can be concluded that the administration of bajakah kalalawit leaf water extract at doses of 52.5 mg/KgBW, 105 mg/KgBW, and 210 mg/KgBW may contribute to histologic changes in the liver and kidney.
Phytochemical Profile and Antioxidant Activity of Bajakah Kalalawit Leaf Extract Priyadi, Muhammad; Gunawan, Yohanes Edy; Saputra, Rizki Rachmad; Decenly, Decenly; Citrariana, Shesanthi
Journal of Experimental and Clinical Pharmacy (JECP) Vol 5, No 1 (2025): February 2025
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v5i1.1321

Abstract

Natural products have long been recognized for their significant role in supporting human health, with natural ingredients playing an essential part in disease treatment and wellness across generations. The bajakah species, particularly bajakah kalalawit (Uncaria gambir Roxb.), has gained attention in recent research due to its potential bioactive compounds. Especially, its antioxidant properties from any part of plant has potential to explore. This study aims to determine the various secondary metabolite compounds possessed by bajakah kalalawit leaves and test their antioxidants. Bajakah kalalawit leaves were extracted using reflux with aqueous solvents, checked for phytochemical profiles using GC-MS, and conducted antioxidant tests using the DPPH method. Based on the phytochemical test, there are 22 predicted compounds in the extract and the antioxidant test results showed an IC50 value of 14.44 ppm. In conclusion, bajakah kalalawit leaf aqueous extract has antioxidant activity and is supported by bioactive compounds.
Aktivitas Psikostimulan Tanaman Bajakah (Spatholobus suberectus) : Studi In Silico Terhadap Protein 4M48-Dopamine Transporter Priyadi, Muhammad; Saputra, Rizki Rachmad; Gunawan, Yohanes Edy; Citrariana, Shesanthi; Decenly, Decenly; Febrianto, Yahya
Jurnal Farmasi & Sains Indonesia Vol 6 No 2 (2023)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52216/jfsi.vol6no2p149-154

Abstract

Bajakah plants (Spatholobus suberectus) have been widely used by Dayak tribes in Kalimantan with various properties that are believed and have also been studied for various tests of phytochemical compound content and pharmacological tests. Secondary metabolite compounds from bajakah (Spatholobus suberectus) have potential as psychostimulant compounds. The purpose of this study was to investigate the psychostimulant activity of bajakah plants (Spatholobus suberectus) through analysis of the interaction between bajakah secondary metabolite compounds and the 4M48-Dopamine Transporter receptor (PDB: 4M48), which is one of the dopamine transporter proteins. Ligand and receptor interactions were analyzed using molecular docking method with Autodock Vina 4 application with nortriptyline compound as native ligand and 15 secondary metabolite compounds for testing. The results of docking analysis showed that the standard ligand nortriptyline compound has an affinity value of -10.2 kcal/mol. There are 3 compounds from bajakah that have affinity values close to the standard compounds plathymenin, butein and eriodictyol with affinity values of -9.7 kcal/mol, -9.4 kcal/mol and -9.2 kcal/mol respectively. Based on the analysis conducted, it can be concluded that bajakah plants (Spatholobus suberectus) have the potential to be developed as psychostimulant therapy.
Improving the Quality of Students of SD Negeri 2 Rantau Pulut Lifestyle Characterized by Clean Water and Renewable Energy: Peningkatan Kualitas Gaya Hidup Siswa SD Negeri 2 Rantau Pulut Bercirikan Air Bersih dan Energi Terbarukan Fatiqin, Awalul; Alfanaar, Rokiy; Rahman, Sudarman; Febrianto, Yahya; Riana, Shesanti Citra; Karyadi, Karyadi; Sahari, Yudhi Ekakristian; Gunawan, Yohanes Edy; Arsana, Mu'afa Purwa; Suprayogi, Thathit
NAWASENA : JOURNAL OF COMMUNITY SERVICE Vol. 1 No. 02 (2023): Vol. 01 No.02 2023
Publisher : NAWASENA : JOURNAL OF COMMUNITY SERVICE

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Clean water is a vital requirement for children at the primary school level as it supports their learning process. The availability of clean water not only facilitates learning activities but is also an important aspect in supporting a lifestyle that is responsible for natural resources, especially in the context of using renewable energy. The emphasis on an appropriate lifestyle in the utilisation of clean water became the focus of the community service conducted at SD Negeri 2 Rantau Pulut. Through this socialisation activity, students were given an understanding of the importance of a clean lifestyle that utilises clean water sources wisely, while integrating the use of renewable energy. The results of this service showed positive progress. Students' understanding of the importance of a clean lifestyle and the utilisation of clean water with renewable energy has increased significantly, reaching an understanding level of 91.67%. From this, it can be concluded that this socialisation successfully achieved its goal of raising students' awareness of the importance of clean water and a sustainable lifestyle.
Peningkatan Keterampilan Guru Biologi SMA Kota Palangka Raya melalui Pelatihan Pembuatan Preparat Histologi Jaringan: Improving the Skills of High School Biology Teachers in Palangka Raya City through Training in Making Tissue Histology Preparations Tuju, Fandi; Gunawan, Yohanes Edy; Wulandari, Meyta
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 8 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i8.7414

Abstract

Tissue histology preparations are the gold standard for determining the pathological state and changes in cells or tissues. Basic tissue histology preparation knowledge is essential for correctly applying tissue processing steps in the histology laboratory. Teachers' skills in making histology preparations need to be improved. The participants in this activity were 12 high school biology teachers in Palangka Raya City who were members of the MGMP Biology Teacher. This activity aims to improve the skills of high school Biology teachers through training in making tissue histology preparations, and it is hoped that this training can provide solutions to Biology practicum in schools, which are certainly packaged to be more interesting, with the advantage that these preparations can be stored and used for an extended period. This activity was carried out at the 2nd Floor Laboratory of the PPIIG Building, Universitas Palangka Raya, in November 2023. The implementation method is training biology teachers to prepare tissue histology. The training began with the delivery of material, followed by discussion, pre-test, training, post-test, and participant testimonials. The results of the post-test assessment showed a significant increase after this training, so it can be concluded that there was an increase in participants' knowledge in making tissue histology preparations.
ECOLOGICAL STUDY OF THE SUNDA PANGOLIN (MANIS JAVANICA DESMAREST, 1822) IN THE NATURE TOURISM PARK OF BUKIT TANGKILING, CENTRAL KALIMANTAN, INDONESIA Lorensi, Monika; Gunawan, Yohanes Edy; Rizki, Muhammad
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.1127

Abstract

The Sunda pangolin, also known as Manis javanica, is a vulnerable mammal that can be found in Java, Sumatra, and Kalimantan. Unfortunately, due to hunting, poaching, land changes, and forest fires, particularly in Kalimantan, this species is now categorized as endangered on the IUCN Red List. To support conservation efforts and sustainability of the Sunda pangolin, it is crucial to gather more evidence about its ecological study and habitat. Therefore, this study aimed to determine the characteristics of the habitat and distribution of the Sunda pangolin in the Bukit Tangkiling Nature Park, Central Kalimantan. We surveyed between February and June 2022 using a Visual Encounter Survey (VES) combined with burrow count methods and camera traps. The survey included vegetation, potential nest and prey, and abiotic factors for the Sunda pangolin. We found a total of two individuals using a camera trap in plot 1 and direct observation in plot 3. Additionally, we located five potential Sunda pangolin nests. According to the distribution map, the Sunda pangolin can be found in hilly and valley areas, with an estimated population of 7.14 individuals/Ha. Vegetation analysis revealed an importance value index (IVI) of 148.84% for Acacia mangium. Based on PCA results, the abiotic factors of air and soil humidity support the existence of the Sunda pangolin and its nests. We also identified eight species of Hymenoptera and one species of Isoptera, which are potential food sources for the Sunda pangolin. Overall, this study provides essential information to support conservation efforts and sustainability of the Sunda pangolin in Kalimantan.
Co-Authors Adventus Panda Agatha, Tiara Alfanaar, Rokiy Citrariana, Shesanthi Decenly, Decenly Fandi Tuju Fatiqin, Awalul Fauziah, Anisa Rahma Gonggoli, Ade Damara Herlina, Rini Karyadi Karyadi Liswara Neneng Lorensi, Monika Meyta Wulandari Muhammad Priyadi Muhammad Rizki Mu’afa Purwa Arsana Oktofiani, Helen Rahman, Sudarman Riana, Shesanti Citra Rizki Rachmad Saputra Sahari, Yudhi Ekakristian Santika, Novira Sartika Sari Sumarsono, Dito Dwi Thathit Suprayogi Yahya Febrianto