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Kamal, Andi Saptaji
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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BIODIVERSITY AND ANTIBACTERIAL ACTIVITIES OF ENDOPHYTIC FUNGI ASSOCIATED WITH UNCARIA GAMBIER (HUNTER) ROXB. VAR. UDANG FROM JASINGA, BOGOR, INDONESIA Palupi, Kartika Dyah; Kamal, Andi Saptaji; Marlina, Lina; Hafid, Lukman; Evana; Tarigan, Elsera Br; Raunsai, Marlin Megalestin; Ilyas, Muhammad; Kanti, Atit; Agusta, Andria
Berita Biologi Vol 22 No 2 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.713

Abstract

Endophytic fungi living in medicinal plant tissues are potential bioactive metabolite sources for drug discovery and development. However, the research regarding endophytic fungi associated with Indonesian medicinal plants is still limited. This study aimed to explore the biodiversity of endophytic fungi associated with the medicinal plant Uncaria gambir (Hunter) Roxb. var. udang and their antibacterial activities. Twenty-seven endophytic filamentous fungi were isolated from the surface-sterilized leaves, stems, and roots of Uncaria gambir (Hunter) Roxb. var. udang collected in Jasinga, Bogor, West Java, Indonesia. The observation based on the morphological characters both macroscopically and microscopically, as well as chemical profiling using thin layer chromatography (TLC), revealed that some of the isolated endophytic fungi were identical. Overall, the isolated endophytic fungi consist of six Coelomycetes, three Dematiaceae, four Pestalotiopsis sp., two Penicillium sp., one Aspergillus terreus, and two fungi were unidentified. Antibacterial activities of the ethyl acetate extract from the isolated endophytic fungi associated with Uncaria gambir (Hunter) Roxb. var. udang were observed against E. coli, B. subtilis, S. aureus, and M. luteus. Twelve types of isolated endophytic fungi from Uncaria gambir (Hunter) Roxb. var. udang exhibited antibacterial activities.
ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ETHYL ACETATE EXTRACT OF ACTINOMYCETES ISOLATED FROM TERMITE NESTS Fathoni, Ahmad; Kamal, Andi Saptaji; Hafid, Lukman; Marlina, Lina; Efendy, Oscar; Putri, Ade Lia; Praptiwi, Praptiwi; Agusta, Andria
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3618

Abstract

Actinomycetes are an important source for the discovery of bioactive secondary metabolite compounds. Over 10,000 bioactive metabolite compounds have been isolated from terrestrial actinomycetes with various biological activities, such as antibiotic, antiviral, anti-inflammatory, antitumor, anticancer, and antioxidant. One source of origin for actinomycetes is soil, including termite nests. Utilization of natural sources such as actinomycetes from termite nests as antioxidants and antibacterials can be an alternative source of production of new secondary metabolite compounds. This research aims to evaluate the antioxidant and antibacterial activities of actinomycete extracts from termite nests. Antioxidant activity using the DPPH free radical scavenging method, and antibacterial activity against S. aureus. Antioxidant and antibacterial activity were performed by using the TLC dot-blot, TLC bioautography, and microdilution methods to determine the inhibitory concentration of 50% (IC50), antioxidant activity index (AAI), and minimum inhibitory concentration (MIC). From the 33 extracts tested, 16 extracts showed antioxidant activity with IC50 values range of 76.64-126.22 µg/mL or AAI value > 0.05 (moderate), and 8 extracts had moderate antibacterial activity against S. aureus (MIC values range of 256-512 µg/mL). Future research for scaling-up of actinomycete culture, isolating of active compounds, determining of the chemical structure of active compounds, and further testing as antioxidants and antibacterials still need to be carried out.
Co-Authors Ahmad Fathoni ANDRIA AGUSTA Atit Kanti Efendy, Oscar Elsera Br Tarigan, Elsera Br Evana Hafid, Lukman Kartika Dyah Palupi lina marlina, lina Muhammad Ilyas Praptiwi Praptiwi Putri, Ade Lia Raunsai, Marlin Megalestin