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All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Berita Biologi
Raunsai, Marlin Megalestin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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DIVERSITY OF FRUIT FLIES (DIPTERA: TEPHRITIDAE) ATTRACTED BY ME LURE IN CSC-BG GERMPLASM CARAMBOLA PLANTATION Riastiwi, Indira; Paradisa, Yashanti Berlinda; Mambrasar, Yasper Michael; Raunsai, Marlin Megalestin; Perwitasari, Urip; Volkandari, Slamet Diah; Sari, Nurul Fitri; Sulistiyani, Tri Ratna; Ibo, Leberina Kristina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221151-157

Abstract

Diversity of fruit flies (Diptera: tephritidae) attracted by me lure in CSG-BG germplasm carambolae plantation. Germplasm Garden (KPN) Cibinong Science Center-Botanical Garden (CSC-BG) is one of the gardens that conserve and utilizes Indonesian local germplasm, one of which is carambolae. The current problem in KPN is the attack of fruit flies (Bactrocera spp.). This study was conducted to determine the diversity of fruit flies in the KPN carambola plantation area. A total of eight traps were placed randomly in the plantation area. Each trap contained 1 mL of methyl eugenol pheromone solution. Identification of fruit flies was carried out based on three morphological differences, they were wings, abdomen, and hind legs. There were 317 fruit flies collected and separated into three species, namely Bactrocera carambolae, B. papayae, dan B. umbrosa, of which B. carambolae had a dominance index of 0.74 and abundance index of 85%. Data on the types of fruit flies can be used as primary data to determine the preparation of pest prevention efforts.
BIODIVERSITY AND ANTIBACTERIAL ACTIVITIES OF ENDOPHYTIC FUNGI ASSOCIATED WITH UNCARIA GAMBIER (HUNTER) ROXB. VAR. UDANG FROM JASINGA, BOGOR, INDONESIA Palupi, Kartika Dyah; Kamal, Andi Saptaji; Marlina, Lina; Hafid, Lukman; Evana; Tarigan, Elsera Br; Raunsai, Marlin Megalestin; Ilyas, Muhammad; Kanti, Atit; Agusta, Andria
Berita Biologi Vol 22 No 2 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.713

Abstract

Endophytic fungi living in medicinal plant tissues are potential bioactive metabolite sources for drug discovery and development. However, the research regarding endophytic fungi associated with Indonesian medicinal plants is still limited. This study aimed to explore the biodiversity of endophytic fungi associated with the medicinal plant Uncaria gambir (Hunter) Roxb. var. udang and their antibacterial activities. Twenty-seven endophytic filamentous fungi were isolated from the surface-sterilized leaves, stems, and roots of Uncaria gambir (Hunter) Roxb. var. udang collected in Jasinga, Bogor, West Java, Indonesia. The observation based on the morphological characters both macroscopically and microscopically, as well as chemical profiling using thin layer chromatography (TLC), revealed that some of the isolated endophytic fungi were identical. Overall, the isolated endophytic fungi consist of six Coelomycetes, three Dematiaceae, four Pestalotiopsis sp., two Penicillium sp., one Aspergillus terreus, and two fungi were unidentified. Antibacterial activities of the ethyl acetate extract from the isolated endophytic fungi associated with Uncaria gambir (Hunter) Roxb. var. udang were observed against E. coli, B. subtilis, S. aureus, and M. luteus. Twelve types of isolated endophytic fungi from Uncaria gambir (Hunter) Roxb. var. udang exhibited antibacterial activities.
ISOLATION AND IN VITRO BIOASSAYS OF ENDOPHYTIC FUNGI ASSOCIATED WITH LIDAH BUAYA (Aloe vera (L.) Burm.f.) Raunsai, Marlin Megalestin; Octavia Siti Nurhayati; Andi Saptaji Kamal; Praptiwi; Kartika Dyah Palupi; Muhammad Ilyas; Rahmawati Ridwan; Evana; Andria Agusta
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3879

Abstract

Endophytic fungi are fungi that live in plant tissues that do not cause disease symptoms, do not harm the host plant, and can produce secondary metabolites such as antibacterial, antifungal, and antiviral compounds. This study was conducted to isolate and evaluate the antibacterial and antioxidant activities of endophytic fungi from Aloe vera (L.) Burm.f. against Staphylococcus aureus and Escherichia coli bacteria. The antibacterial activity test was carried out qualitatively by thin layer chromatography-direct-bioautography (TLC-DB), and microdilution methods were used for the antibacterial assay against Staphylococcus aureus and Escherichia coli. Antioxidant activity was determined against DPPH using TLC-DB and the microdilution assay. Antibacterial results showed that the extracts LBB-2.4, LBB-2.2, LBAu-1.1 and LBAu-2.1 could inhibit the growth of S. aureus. Meanwhile, extracts of endophytic fungi with the isolate codes LBB-2.4, LBB-2.1, LBAp-3, LBAp-1, LBAu-1.1 and LBAu-2.1 could inhibit the growth of E.coli. The results of Minimum Inhibitory Concentration (MIC) showed that isolate LBB-2.1 had moderate antibacterial activity with a MIC value of 256 μg/mL against S.aureus. The extracts showed weak antioxidant activity against DPPH. It can be concluded that endophytic fungi extracts from Aloe vera have potential activity as antibacterial agents.
Co-Authors Andi Saptaji Kamal ANDRIA AGUSTA Atit Kanti Elsera Br Tarigan, Elsera Br Evana Hafid, Lukman Ibo, Leberina Kristina Kamal, Andi Saptaji Kartika Dyah Palupi Kartika Dyah Palupi lina marlina, lina Muhammad Ilyas Muhammad Ilyas Octavia Siti Nurhayati Paradisa, Yashanti Berlinda Praptiwi Rahmawati Ridwan Riastiwi, Indira Sari, Nurul Fitri Slamet Diah Volkandari, Slamet Diah TRI RATNA SULISTIYANI Urip Perwitasari, Urip Yasper Michael Mambrasar, Yasper Michael