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HERITABILITY ANALYSIS OF LOCAL CORN CULTIVARS FROM KISAR ISLAND SOUTHWEST MALUKU AFTER INDUCED WITH COLCHICINE Sinay, Hermalina; Tanrobak, Jonetha
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.183 KB) | DOI: 10.15294/biosaintifika.v12i1.19847

Abstract

In the plant breeding program, selection is one process which aimed to obtain some varieties with superior traits. Within this process, one of criteria that must be consider is the wide genetic diversity of the plant which was selected, and how these traits are inherited. The purpose of this research was to determine (1) the influence of cultivar differences and the colchicine concentration towards the growth and production of local corn cultivar from Kisar Island Maluku, (2) to determine the heritability of growth and production variables after induced with colchicine. Completely randomized block design  was used in this research with factorial design that consist of two factors i.e corn cultivar and colchicine concentration. The research procedure starts from field and seed preparation, immersion of seed into the colchicine solution, transplantation of seed into the field, plant maintenance, and  harvesting. The variable measured including growth and production variables of corn. The analysis of variance showed a significant effect of cultivar differences on all growth and production variables (p value > 0.05), while colchicine concentration only showed a significant effect toward the plant height and number of seed per ear.  The heritability analysis result showed that all variables observed were in high category. The different corn cultivars have a significant effect on all the growth and production, while the colchicine concentration only affect the plant height and number of seed per ear, and the heritability were in high category.  This research provides useful information to farmers how to use colchicine to trigger plant growth and production. It can be used as an information in selecting corn cultivars with high yield potential.
Screening of Bioactive Compounds of the Brown Algae Turbinaria ornata (Turner) J. Agardh, 1848 From The Coastal Waters of Aboru Village, Central Maluku Indonesia Sinay, Hermalina; El-sheekh, Mostafa
Pharmaceutical Journal of Indonesia Vol. 9 No. 1 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2023.009.01.3

Abstract

Turbinaria ornata belonging to brown algae is a leading community in the sea water ecosystems which is rich in nutrients and bioactive compounds. The bioactive compounds of T. ornata are useful for medicine, pharmaceutical, industries and for people in the coastal area especially in Maluku Province as an archipelagic province. This study provides information about the importance and beneficial value of T. ornata and determines the bioactive compounds of T. ornata from in coastal waters of Aboru Village Central Maluku Regency. Screening of bioactive compounds was conducted qualitatively consisting of phenolics, tannins, flavonoids, saponins, triterpenoids, steroids, alkaloids and anthocyanins.The result of this study showed that qualitative screening of bioactive compounds of brown algae T. ornata positive for tannins, saponins, alkaloids, steroids, phenolics, and flavonoids, while triterpenoids, quinones, and anthocyanins were negative. Based on this research, it can concluded that T. ornata contains some bioactive compounds including tannins, saponins, alkaloids, steorids, phenolics, and flavonoids.
Screening of Bioactive Compounds of the Brown Algae Turbinaria ornata (Turner) J. Agardh, 1848 From The Coastal Waters of Aboru Village, Central Maluku Indonesia Sinay, Hermalina; El-sheekh, Mostafa
Pharmaceutical Journal of Indonesia Vol. 9 No. 1 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2023.009.01.3

Abstract

Turbinaria ornata belonging to brown algae is a leading community in the sea water ecosystems which is rich in nutrients and bioactive compounds. The bioactive compounds of T. ornata are useful for medicine, pharmaceutical, industries and for people in the coastal area especially in Maluku Province as an archipelagic province. This study provides information about the importance and beneficial value of T. ornata and determines the bioactive compounds of T. ornata from in coastal waters of Aboru Village Central Maluku Regency. Screening of bioactive compounds was conducted qualitatively consisting of phenolics, tannins, flavonoids, saponins, triterpenoids, steroids, alkaloids and anthocyanins.The result of this study showed that qualitative screening of bioactive compounds of brown algae T. ornata positive for tannins, saponins, alkaloids, steroids, phenolics, and flavonoids, while triterpenoids, quinones, and anthocyanins were negative. Based on this research, it can concluded that T. ornata contains some bioactive compounds including tannins, saponins, alkaloids, steorids, phenolics, and flavonoids.
Characterization of Starch Granaule of Local Water Yam (Dioscorea alata L.) from Lermatang, Tanimbar Island District, Maluku: Characterization of Starch Granule of Dioscorea alata L. Sinay, Hermalina; Karuwal, Ritha Lusian; Fauziah, Fauziah; Anaktototy , Helena
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.18

Abstract

Most tuberous crops, for instance, the yam, whose scientific name is Dioscorea alata, have food reserves in the form of starch in tubers. Characterization of starch grains of a plant species is pertinent to uncover information on plant taxonomic traits and identify their potential use as food and industrial raw materials. The study aimed to determine the characteristics of starch grains of water yam from Lermatang village, Tanimbar Islands Regency. This study isolated and submitted six accessions of water yam in Lermatang Village. The morphological inspection of the plants used the Descriptor for Yam from IPGRI, and sample preparation for observing starch grains employed the fresh section method. The six accessions of D. alata found in Lermatang village, Tanimbar Islands, were Uwi Merah, Uwi Gula, Uwi Pingingsian, Uwi Babulu, Uwi Akiakab, and Uwi Petatas. These yam varieties show differences in their tubers' shape and colors. The study found that starch granules of D. alata in each accession varied in terms of granule shape and hilus type. The starch granules revealed dominant triangular, oval, and elliptical shapes and eccentric and concentric hilus types. The starch grain diameter in the six accessions of Uwi was categorically large (44.88 – 57.5 µm), with Uwi Petatas being the largest. Previous reports have shown that accessions having large starch granule types could be further developed into an array of foods, thus conveying the promising use of the Uwi Petatas for such a purpose.
KARAKTERISASI STOMATA DAUN JERUK KALAMANSI (Citrus microcarpa Bunge.) DI PULAU AMBON Rahangmetan, Agustina; Sinay, Hermalina; Karuwal, Ritha Lusian
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 7 No 2 (2021): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol7issue2page180-192

Abstract

Latar Belakang: Jeruk kalamansi (Citrus microcarpa Bunge.) adalah buat tropika yang sangat popular di Ambon dan Maluku. Buah ini memiliki cita rasa asam sehingga menambah aroma khas pada berbagai campuran masakan. Sejauh ini, belum banyak laporan yang dipublikasikan terkait sifat-sifat anatomi daun khususnya stomata daun jeruk kalamansi pada lokasi-lokasi berbeda Pulau Ambon. Dalam kaitan dengan kemampuan adaptasi terhadap lingkungan, maka sifat-sifat tanaman termasuk sifat anatomi dan struktur stomata pada daun, penting untuk diketahui. Metode: koleksi sampel dilakukan dengan metode accidental sampling. Pembuatan preparat untuk pengamatan stomata dilakukan dengan metode whole mount. Pengamatan dilakukan terhadap tipe, jumlah, kerapatan dan indeks stomata. Data yang diperoleh adalah rerata tiga ulangan dan disajikan secara deskriptif dalam bentuk tabel dan gambar. Hasil : hasil penelitian menunjukkan bahwa stomata daun jeruk Kalamansi yang dikoleksi dari 13 lokasi berbeda di Pulau Ambon memiliki dua tipe stomata yaitu anemositik, siklositik dan campuran antara keduanya. Jumlah, kerapatan dan indeks stomata tertinggi diperoleh ada sampel daun dari Desa Kilang, dan terendah pada Desa Latta. Pada lokasi yang lain, bervariasi dan hal jumlah, kerapatan dan indeks stomatanya, di mana ada lokasi yang jumlah stomata lebih tinggi dari indeks dan kerapatan, dan juga sebaliknya. jumlah stomata secara berurutan dari tertinggi ke terendah yaitu Desa Kilang (32,71), Hutumuri (31,11), Lateri (29,15), Naku dan Passo (27,8), Wayame (26,43), Hative Besar (26,58), Halong (25,50), Hukurila (24,52), Poka (23,51), Kusu-Kusu Sereh (22,36), Rumahtiga (21,63), dan Wayame (14,47). Untuk Indeks Stomata secara berurutan dari tertinggi ke terendah yaitu: Kilang (48,05), Wayame (46,71), Lateri (43,92), Hutumuri (42,37), Naku (42,12), Hative Besar (41,18), Poka (40,89), Kusu-Kusu Sereh (40,64), Halong (39,45), Hukurila (37,84), Passo (36,58), Rumahtiga (36,42), dan Latta (35,73). Untuk kerapatan stomata, berturut-turut dari tertinggi ke terendah yaitu Kilang (0,32), Hative Besar (0,31), Wayame (0,29), Poka (0,28), Lateri (0,27), Halong (0,24), Passo, Hutumuri, Hukurilla (0,23), Naku (0,22), Kusu-Kusu Sereh dan Rumahtiga (0,21) dan Latta (0,14). Kesimpulan: Tipe stomata pada daun jeruk kalamansi dari 13 lokasi di Pulau Ambon yaitu anomositik, dan siklosistik. Jumlah, kerapatan dan indeks stomata tertinggi diperoleh ada sampel daun dari Desa Kilang, dan terendah pada Desa Latta
Tannin Content of Lindur Fruit Flour (Bruguiera gymnorrhiza) Following Long Soaking deLima, Mechindy; Sinay, Hermalina; Kurnia, Tri Santi
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 9 No 1 (2022): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol9issue1page125-131

Abstract

Background: Lindur is a type of mangrove (Bruguiera gimnorrhiza). The fruit of this type of mangrove can be used to make flour. Lindur fruit is edible because it contains nutrients like carbohydrates, fats, and proteins. Lindur contains secondary metabolite compounds such as tannins in addition to its nutritional content. The presence of tannins is frequently a barrier to lindur consumption. Soaking reduces the tannin levels in lindur fruit. The goal of this study was to see how soaking time affected the tannin content of lindur fruit flour. Methods: This experiment uses Completely Randomized Design with three (3) treatments. The treatment duration of immersion is 24 hours, 36 hours, and 72 hours, with each repetition occurring three times. Lindur fruit samples were obtained from the mangrove forest of Osi Island, West Seram Regency. Tannin content analysis was carried out with spectroscopy method. At the 0.05 level, data were analyzed using analysis of variance and Duncan's multiple distance test. Results: Tannin levels in lindur fruit flour (B. gymnorrhiza) at 24, 36, and 72 hours of immersion, respectively, were 3,000 mg/kg, 2,647 mg/kg, and 2.188 mg/kg. The 72-hour treatment was the most effective at lowering tannin levels. The water immersion treatment reduced tannin levels in B. gymnorrhiza lindur fruit significantly (p 0.05). Conclusion: The number of tannins reduced in lindur fruit flour produced by B. Gimnorrhiza is proportional to the length of immersion time
KADAR KLOROFIL DAUN PAKCOY (BRASSICA CHINENSIS L.) SETELAH PERLAKUAN PUPUK KANDANG DAN AMPAS TAHU SEBAGAI BAHAN AJAR MATA KULIAH FISIOLOGI TUMBUHAN Wenno, Stenly Jacob; Sinay, Hermalina
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 5 No 2 (2019): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol5issue2page130-139

Abstract

Background: Pakcoy (Brassica chinensis L.) is a vegetable plant originating from China that has leaf stalks that are wider and larger than green mustard greens. Pakcoy has nutritional value such as vitamin C, vitamin A, carbohydrates, fiber, protein, calcium, magnesium. Chlorophyll is a green leaf substance found in green parts of plants, especially on leaves. One of the factors that influence the chlorophyll content in pakcoy mustard leaves is the soil condition in this case the nutrient content and nutrients in the soil. One way to meet nutrient and nutrient content in the soil is by using organic fertilizers such as manure and tofu. Method: This study aims to determine the chlorophyll content of pakcoy leaves (Brassica chinensis L.) after giving manure and tofu dregs. Based on the analysis of chlorophyll content using the UV-VIS spectrophotometer method at wavelengths of 644 nm and 663 nm. Results: The highest levels of chlorophyll A in pakcoy (Brassica chinensis L.) leaves with PK3AT1 treatment (7 kg manure and 7 kg tofu dregs) of 2.007 mg / L, highest chlorophyll B levels in pakcoy (Brassica chinensis L.) leaves with treatment PK3AT3 (7 kg manure and tofu dregs 3 kg) at 2.674 mg / L, and the highest total chlorophyll content in pakcoy (Brassica chinensis L.) leaves with PK3AT1 treatment (7 kg manure and 7 kg tofu dregs) at 4,558 mg / L. Conclusion: Chlorophyll A, B and total levels in pakchoy (Brassica chinensis L.) leaves after a combination of manure and tofu dregs have high levels of different corophils.
Characterization of Starch Granaule of Local Water Yam (Dioscorea alata L.) from Lermatang, Tanimbar Island District, Maluku: Characterization of Starch Granule of Dioscorea alata L. Sinay, Hermalina; Karuwal, Ritha Lusian; Fauziah, Fauziah; Anaktototy , Helena
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.18

Abstract

Most tuberous crops, for instance, the yam, whose scientific name is Dioscorea alata, have food reserves in the form of starch in tubers. Characterization of starch grains of a plant species is pertinent to uncover information on plant taxonomic traits and identify their potential use as food and industrial raw materials. The study aimed to determine the characteristics of starch grains of water yam from Lermatang village, Tanimbar Islands Regency. This study isolated and submitted six accessions of water yam in Lermatang Village. The morphological inspection of the plants used the Descriptor for Yam from IPGRI, and sample preparation for observing starch grains employed the fresh section method. The six accessions of D. alata found in Lermatang village, Tanimbar Islands, were Uwi Merah, Uwi Gula, Uwi Pingingsian, Uwi Babulu, Uwi Akiakab, and Uwi Petatas. These yam varieties show differences in their tubers' shape and colors. The study found that starch granules of D. alata in each accession varied in terms of granule shape and hilus type. The starch granules revealed dominant triangular, oval, and elliptical shapes and eccentric and concentric hilus types. The starch grain diameter in the six accessions of Uwi was categorically large (44.88 – 57.5 µm), with Uwi Petatas being the largest. Previous reports have shown that accessions having large starch granule types could be further developed into an array of foods, thus conveying the promising use of the Uwi Petatas for such a purpose.
EDUKASI PEMBUATAN PRODUK PANGAN BERBAHAN DASAR KACANG TUNGGAK LOKAL BAGI MASYARAKAT DESA TAAR-KOTA TUAL Karuwal, Ritha Lusian; Sinay, Hermalina; Maturbongs, Agustina
Jurnal Abdi Insani Vol 12 No 2 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i2.2293

Abstract

Kepulauan Kei memiliki sumber daya lokal yang berpotensi dikembangkan, salah satunya adalah kacang tunggak yang dikenal dengan sebutan kacang merah. Kacang tunggak ini ditemukan di bagian Tenggara dan Barat Daya dari Provinsi Maluku dengan kandungan nutrisi yang tinggi dan dapat dikembangkan sebagai bahan baku pembuatan makanan alternatif. Selama ini, pemanfaatan kacang tunggak oleh masyarakat setempat hanya sebagai bahan pelengkap karbohidrat yang diolah secara tradisional dalam bentuk nasi maupun bubur.  Kebiasaan mengkonsumsi seperti ini telah berlangsung bertahun-tahun lamanya. Inovasi pengembangan belum pernah dilakukan.  Hal ini disebabkan kurangnya kesadaran dan pengetahuan masyarakat untuk mengembangkan pemanfaatan kacang merah sehingga dapat memenuhi kebutuhan gizi dan meningkatkan kesejahteran. Dengan memanfaatkan kacang tunggak melalui penepungan atau tanpa penepungan dapat dibuat produk pangan alternatif seperti tempe, susu, pasta, puding, bakpao, cookies. Dengan demikian, perlu adanya upaya edukasi untuk memberikan solusi alternatif yang mampu meningkatkan keterampilan dan kreatifitas masyarakat, khususnya yang di Desa Taar Kota Tual Kepulauan Kei. Metode yang dipakai dalam kegiatan ini adalah edukasi bagi kelompok perempuan sebanyak 30 orang. Kegiatan edukasi berupa sosialisasi dan pelatihan atau demonstrasi pembuatan produk pangan berbahan dasar kacang tunggak lokal oleh peserta. Dalam kegiatan pengabdian ini peserta sangat antusias. Hal ini ditunjukkan dari keseriusan peserta dalam mengikuti setiap kegiatan baik penyampaian materi oleh pelaksana maupun uji coba atau demo oleh peserta. Peserta juga terampil dalam membat produk sesuai arahan dari tim pelaksana. Dengan demikian, kacang tunggak sangat berpeluang untuk dikembangkan dan dikomersialkan sebagai produk pangan.
Trichomes Characterization of Ornamental Plants Begonia spp (Begoniaceae) Hakapaa, Adriana; Sinay, Hermalina; Karuwal, Ritha Lusian; Gusmalawati, Dwi
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.21854

Abstract

The presence of sharp needle-shaped hairs on the leaf surface of the ornamental begonia plants is recognized as a trichome that has not been widely studied. This research aimed to determine the characteristics of trichomes of Begonia’s leaves in Ambon. The plant material used was the Begonia species that was explored in Ambon and private collections. Identification of plant species, scientific and trade names was carried out by comparison of the sample plants' characteristics with online data and references. Trichome observations were carried out using the whole mount method with 400x magnification using the Optilab camera of the Olympus microscope. Trichome’s shape and type were described based on the obtained pictures, while number and length were the average of 10 fields of view, from 3 replications, and displayed as mean ± standard deviation (mean ± SD).  The 21 Begonia species were collected, and their trichome was characterized. In general, the Begonia’s trichome consists of structure like base, median or body and tip, uniseriate and multiseriate types, needle or cone shape, and glandular or non glandular. The average length of trichomes range from 29.80-233.47 µm.  Based on these results, it can be concluded that the trichomes on the leaves of Begonia ornamental plants in Ambon City have variations in type, shape, number and size. This result is expected to be new information or references for those who interest in developing Begonia species, especially in classifying begonias based on their trichomes characteristics.