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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Biosaintifika: Journal of Biology & Biology Education JURNAL ILMIAH AGRINECA Jurnal Litbang Provinsi Jawa Tengah Agricultural Science GANESHA: Jurnal Pengabdian Masyarakat PROFICIO: Jurnal Pengabdian Masyarakat Journal of Applied Agricultural Science and Technology Journal of Community Capacity Empowerment Biosaintifika: Journal of Biology & Biology Education
Dwi Susilo Utami
Universitas Tunas Pembangunan Surakarta
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Materials Science & Nanotechnology Public Health Social Sciences Other

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Journal : JURNAL ILMIAH AGRINECA

 1 
EFEKTIFITAS RHIZOCTONIA MIKORIZA DALAM MENGINDUKSI KETAHANAN ANGGREK PHALAENOPSIS AMABILIS TERHADAP FUSARIUM SP. R. Soelistijono
JURNAL ILMIAH AGRINECA Vol. 14 No. 2 (2014): JURNAL ILMIAH AGRINEÇA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v14i2.287

Abstract

This study examines the effectiveness of mycorrhizal Rhizoctonia resistance induction in Phalaenopsis amabilis against Fusarium sp. Fusarium solani is known as pathogens that attack many orchids P. amabilis (Chung et al., 2011) compared to other pathogenic fungi. Attack of Fusarium sp. will cause rot and yellow colored leaves. Until now there has been known as a biological control orchid against Fusarium sp. In this study tested the endurance locations in Sleman and Surakarta to see the effectiveness of a good orchid growth induced by Rhizoctonia mycorrhizal or not to attack by Fusarium sp. The results of the study showed that mycorrhizal Rhizoctonia able to inhibit the attack of Fusarium sp. It is shown by the value of the index of disease resistance  (DSI) in P. amabilis orchid mycorrhizal Rhizoctonia induced lower than that not induced. Mycorrhizal Rhizoctonia induction results in Sleman provide a more real than mycorrhizal Rhizoctonia induction in Surakarta.
ISOLASI DAN IDENTIFIKASI RHIZOCTONIA MIKORIZA ANGGREK VANDATRICOLORDARI KOPENG JAWA TENGAH Muhamad Zumri; DARYANTI DARYANTI; R. Soelistijono
JURNAL ILMIAH AGRINECA Vol. 17 No. 1 (2017): JURNAL AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v17i1.560

Abstract

This study aims to identify mycorrhizal Rhizoctonia, morphology and anatomy, and determine the association of mycorrhizal Rhizoctonia among Vanda tricolor orchid from Kopeng Central Java with Vanda limbata from Malang, Jawa Timur. The research was held in June 2016 until December 2016 at Universitas Tunas Pembangunan Surakarta’s Laboratory. This research used direct observation by isolating the root of Vanda tricolor orchid and Vanda limbata orchid that has growth. The research stages are sampling, sterilization, isolation, purification, identification, data analysis. From the results of this study indicate: (1) there are no differences in mycorrhizal Rhizoctoniashape morphology and anatomy at the root of Vanda tricolor orchid and Vanda limbata orchid. (2) There is a peloton that is associated with the mycorrhizal Rhizoctonia on the roots of Vanda tricolor orchid and Vanda limbata orchid. (3) There are differences in the location of the peloton on the roots of Vanda tricolororchid and Vanda limbata orchid.
KARAKTERISASI MORFOLOGI DAN ANATOMI ISOLAT RHIZOCTONIA MIKORIZA ANGGREK VANDA LIMBATA WAHYU DWIYANTO; R. Soelistijono; DWI SUSILO UTAMI
JURNAL ILMIAH AGRINECA Vol. 17 No. 1 (2017): JURNAL AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v17i1.563

Abstract

Rhizoctonia mycorrhizal fungi is a group that has a very important role for the plant anggrekan. Endophyte orchid belonging to the genus Rhizoctonia that consists of three species, namely R. repens, mucoroides R., and R. lanuginose. Rhizoctonia fungus colony morphology sp. contained in preparations or isolates no difference because the preparations are equally white as found in isolates. Different characteristics of the mycorrhizal Rhizoctonia root Vanda limbata Flores seen by colony color hyphae, and the number of cell nuclei. Rhizoctonia mycorrhizal hyphae colonies on the roots of the orchid Vanda limbata has mycelium is brown, there are barriers hyphae (septa) therein, and some have 2 cell nucleus. Mycelium Rhizoctonia sp. in isolates Vanda limbata is large While colonies hyphae Rhizoctonia sp. in isolates Vanda limbata known paler brown, do not have septa, and has a core of more than 1. The mycelium Rhizoctonia sp. on the roots of Vanda limbata which tend to tan and smaller. It can be said mycorrhizal Rhizoctonia contained in Vanda limbata included in binukleat like R. repens group that includes orchid mycorrhizal fungi. While mycorrhizal Rhizoctonia contained in Vanda limbata including nature groups such multinucleate R. solani, T. cucumeris, T. pennatus contained orchids. Binukleat or multinucleate Rhizoctonia group as good because it is as mycorrhizae
PENGARUH APLIKASI RHIZOCTONIA MIKORIZA DAN INTERVAL WAKTU PEMBERIAN AIR PADA PERTUMBUHAN SEEDLING ANGGREK Dendrobium stockelbuschii Schettler Setie Herieni; R. Soelistijono; Sari M; T Supriyadi; Daryanti; T.S. Kurnia Dewi
JURNAL ILMIAH AGRINECA Vol. 21 No. 2 (2021): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v21i2.1479

Abstract

Orchid was an ornamental plant cultivated by tissue culture (in vitro). This was because the seeds in orchid plants do not have food reserves (endosperm) to support their growth, so the plants only get nutrients and nutrients from the culture media. The propagation of orchid plants by tissue culture causes these plants do not naturally have Rhizoctonia mycorrhiza so that further plant growth in nature vegetatively and generatively will not be optimal. In this study, Rhizoctonia mycorrhiza was inoculated from species of orchids on seedling orchids of different species with longer watering intervals (2 days, 4 days, and 6 days). The method used was Randomized Block Design (RAK). The results obtained showed that Rhizoctonia mycorrhiza had 2 cell nuclei and had branches with right angles. The application treatment of Rhizoctonia mycorrhiza (M) had a very significant effect on the parameters of plant height growth, the number of leaves, and fresh weight. The treatment of watering time interval (P) had a very significant effect on the parameters of plant height growth, the number of leaves, and plant fresh weight, and affected the number of roots. The combination of Rhizoctonia mycorrhiza application treatment and water application time interval (M x P) showed results that had a very significant effect on the number of leaves parameters and significantly affected plant height. Rhizoctonia mycorrhiza applied to D. stockelbuschii was able to form associations with plant roots by forming a platoon structure.
Mikoriza Rhizoctonia : Sebagai Agen Ketahanan Pada Anggrek Dendrobium Soelistijono, R.; Supriyadi, Teguh; Tiara Maharani, Annissa; Imani Fatihah, Syalaysa
JURNAL ILMIAH AGRINECA Vol 25 No 1 (2025): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v25i1.4612

Abstract

Drought and low rainfall are natural phenomena that occurred in 2022-2023. These factors (abiotic factors) caused diseases in orchids, which affect the metabolism of growth and the flowering process, making the plants unable to survive in their habitat because they cannot reproduce, thus classifying them under Appendix 2 (CITES). However, some orchid species that are endemic are able to withstand drought and low rainfall. It is suspected that the orchids' ability to endure these factors is due to the role of endophytic fungi (Rhizoctonia binucleate / BNR), which can associate with orchids and produce secondary metabolites that help supply nutrients to the orchids during drought stress. This study aims to evaluate the biological resistance of different strains of Rhizoctonia binucleate / BNR isolates in Dendrobium orchids to understand their impact on water stress. The research method used a completely randomized design with a single stage consisting of two factors and five replications. Factor 1: orchid seedling types consisting of S1 = Phalaenopsis violaceae (epiphytic orchid) and S2 = Dendrobium aggregatum (epiphytic orchid). Factor 2: types of Rhizoctonia mycorrhiza application consisting of K0 = control, K1 = Rhizoctonia mycorrhiza application.
Respon Bawang Merah Varietas Bima Terhadap Aplikasi Mikoriza dan Pupuk Organik Cair Limbah Rumah Tangga: Respon Bawang Merah Varietas Bima Terhadap Aplikasi Mikoriza dan Pupuk Organik Cair Limbah Rumah Tangga Daryanti; Soelistijono, R.; Mardhika Sari, Siti; Soemarah Kurnia Dewi, Tyas; Restu Putra Abadi, Pramudika
JURNAL ILMIAH AGRINECA Vol 25 No 2 (2025): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v25i2.5387

Abstract

Shallots are a horticultural commodity whose demand is always increasing. Organic cultivation by utilizing household waste as organic fertilizer and mycorrhizae is one way to increase the production of Bima variety shallots. The study was conducted from February to May 2025 in Koripan Village, Matesih District, Karanganyar Regency. This study aimed to determine the effect of mycorrhizae and liquid organic fertilizer (POC) from household waste on the growth and yield of Bima variety shallots. The study was conducted using a Completely Randomized Block Design (RAKL) using two factors, namely the mycorrhizae dose (0, 5, and 10 grams) and the POC watering interval (0, 3, 6, and 9 days). The results showed that the administration of 10 grams of mycorrhizae gave the best results in terms of bulb weight (32.75 grams), number of bulbs (7.20), and consumption bulb weight (31.12 grams). Meanwhile, the three-day POC watering interval (P2) resulted in the highest tuber weight (36.83 grams), tuber number (8.00 grams), and tuber consumption weight (35.44 grams) of all treatments. The combination of mycorrhizal treatment and POC watering interval significantly increased plant productivity in an organic and environmentally friendly manner.
Morphological and Anatomical Identification of Rhizoctonia Mycorrhaza From Four Species of Phalaenopsis sp.: Morphological and Anatomical Identification of Rhizoctonia Mycorrhaza From Four Species of Phalaenopsis sp. Soelistijono, R.; Tiara Maharani, Annissa; Daryanti; Suprapti, Endang
JURNAL ILMIAH AGRINECA Vol 26 No 1 (2026): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta (UTP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v26i1.6036

Abstract

Rhizoctonia mycorrhiza is an endophytic fungus that plays an important role in orchid growth and can increase plant resistance to environmental stress and pathogens. The purpose of this study was to examine the morphology and anatomy of Rhizoctonia mycorrhiza isolated from four Phalaenopsis orchid species. The study used a quantitative descriptive method through direct observation and analysis of the percentage of hyphal cell nuclei. The results showed that the four Rhizoctonia mycorrhiza isolates had different colony morphological characteristics. Isolates from Phalaenopsis venosa had brownish-white colonies with rapid mycelial growth, isolates from Phalaenopsis amabilis showed grayish-white colonies with thinner growth and Phalaenopsis celebensis, colonies were white-brown and began to form dark masses suspected to be sclerotia. Meanwhile, isolates from Phalaenopsis gigantea showed white-dark green colonies with a circular growth pattern and rapid spread. Anatomical observations showed that all isolates had the typical characteristics of Rhizoctonia, namely hyphal branching at a 90° angle, septate hyphae, and the presence of more than one cell nucleus. Based on the count of 120 hyphal cells, isolates from P. venosa (63.3%), P. amabilis (66.6%), and P. gigantea (60%) were dominated by binucleate cells, while isolates from P. celebensis (70%) were dominated by multinucleate cells. This indicates the physiological diversity of Rhizoctonia mycorrhiza among orchid species. Thus, this study confirms that the roots of four species of Phalaenopsis orchid species contain Rhizoctonia mycorrhiza with morphological and anatomical variations that can be used as an inoculum source for the development of orchid cultivation as well as further research related to its physiological function and application as a biological agent.
Tinjauan Artikel : Jamur Endofit Dari Akar Anggrek (Orchidaceae): Tinjauan Artikel : Jamur Endofit Dari Akar Anggrek (Orchidaceae) Soelistijono, R.
JURNAL ILMIAH AGRINECA Vol 26 No 1 (2026): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta (UTP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v26i1.6228

Abstract

Orchids, renowned for their beauty and diverse medicinal properties, are increasingly threatened by habitat destruction and illegal trade, driving many species to extinction. To better understand and conserve these fascinating plants, research into their symbiotic relationships with endophytic fungi (OEF) has become a major focus. This review paper examines the multifaceted roles of endophytic fungi in orchids, including seed germination, stress tolerance, secondary metabolite production, nutrient acquisition, and in vitro propagation. Orchid endophytic fungi play a crucial role in orchid growth and can enhance plant resistance to environmental stresses and pathogens. Endophytic fungi play a crucial role in facilitating orchid seed germination and growth by providing essential nutrients and enhancing stress tolerance. Furthermore, these fungi produce bioactive compounds with significant pharmaceutical potential, underscoring their importance in drug discovery. Furthermore, endophytic fungi enhance nutrient uptake and support micropropagation efforts, thus contributing to orchid conservation. Understanding the complex interactions between orchids and endophytic fungi is crucial for promoting sustainable floriculture, conserving biodiversity, and improving human well-being.
Co-Authors Achmad Fatchul Aziez Agus Budiyono Alanusa, Awang Arif Rohman Hakim Daryanti DARYANTI DARYANTI Dian Rakhmawati, Dian Dwi Susilo Utami Endang Suprapti HARTOYO, EKO Haryuni Haryuni , Haryuni Haryuni, H. Imani Fatihah, Syalaysa Joko Sulistyono KODRAT BUDIYONO Luncana Faridhoh Sasmito Mardhika Sari, Siti Moh. Erkamim Muhamad Zumri Nugroho, Benny Prasetio, Suji Tegar Priyadi, Sapto Priyadi Restu Putra Abadi, Pramudika Rianto, Prisma Aditya Rosa Suryantini Sapto Priyadi Sari M Setie Harieni Setie Herieni Shodiq, Yusuf Nur Sholkhan, Ahmad Siti Mardhika Sari Soemarah Kurnia Dewi, Tyas Subarkah, Muhammad Zidni Sukamto T Supriyadi T.S. Kurnia Dewi Teguh Supriyadi Tiara Maharani, Annissa Totong Umar Tyas Soemarah K.D Utomo, Herry WAHYU DWIYANTO Wibowo, Maulana Tulus Wiyono wiyono