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Statistical analysis of agarwood oil chemical compound exists in four species of Aquilaria Zaidi, Amir Hussairi; Al-Hadi, Anis Hazirah ‘Izzati Hasnu; Huzir, Siti Mariatul Hazwa Mohd; Yusoff, Zakiah Mohd; Ismail, Nurlaila; Almisreb, Ali Abd; Tajuddin, Saiful Nizam; Taib, Haji Mohd Nasir
International Journal of Advances in Applied Sciences Vol 13, No 3: September 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v13.i3.pp727-732

Abstract

Aquilaria, renowned for its agarwood, and valued for its aromatic wood and rich resin, finds use in cosmetics, fragrances, incense, and medicine. Identifying the agarwood-producing species among 21 species of Aquilaria is challenging. This study analyzes chemical compounds in agarwood oil from 4 Aquilaria species: Aquilaria beccariana, Aquilaria crassna, Aquilaria malaccensis, and Aquilaria subintegra using gas chromatography-flame ionization detector (GC-FID). Statistical analysis explores compound abundance, employing methods like mean and Z-score tests. This analysis summarizes those 14 compounds that are consistently present based on zero and non-zero observations, and the Z-score test highlights five chemical compounds, with three compounds appearing in both analyses. These compounds can serve as a reference for future studies on Aquilaria species and agarwood oil, enhancing classification efforts.
Chemometric classification and authentication of four Aquilaria species from essential oil profiles using GC-MS/GC-FID and ANN Noramli, Nur Athirah Syafiqah; Ahmad Sabri, Noor Aida Syakira; Roslan, Muhammad Ikhsan; Ismail, Nurlaila; Mohd Yusoff, Zakiah; Taib, Mohd Nasir
International Journal of Advances in Intelligent Informatics Vol 11, No 4 (2025): November 2025
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/ijain.v11i4.2141

Abstract

Agarwood derived from Aquilaria species is among the most valuable aromatic resources, yet frequent species misidentification hampers conservation efforts and compliance with trade regulations. This study applied a chemometric ANN framework to classify four Aquilaria species (A. malaccensis, A. beccariana, A. subintegra, and A. crassna) based on essential oil composition. A total of 720 samples (180 per species, each analyzed in triplicate) were extracted via hydro-distillation and profiled using GC–MS coupled with GC–FID. Six compounds were consistently detected, and three (δ-guaiene, 10-epi-γ-eudesmol, γ-eudesmol) were retained for classification based on ≥95% detection frequency and >0.2% relative abundance. Pearson correlation guided feature selection, and ANN models were trained using both a 70:15:15 train–validation–test split and stratified 5-fold cross-validation with 1000 bootstrap resamples. As shown in Tables 5 and 6, the optimized network achieved near-perfect performance with mean accuracy of ~99.8% (95% CI: 99.6–100.0) and precision, recall, and F1-scores all exceeding 99.5%, while bootstrapped confidence intervals were tightly bounded at 100%, confirming robustness against data leakage. These findings demonstrate that correlation-guided feature selection combined with ANN modeling enables reproducible and highly accurate species authentication, offering a practical framework for integration into agarwood quality control, conservation monitoring, and international trade compliance.