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Journal : Indonesian Journal of Global Health research

The Relationship of HBA1C with Mean Platelet Volume (MPV) in Type 2 Diabetes Mellitus Patients Sahid, Muhammad; Saroh, Dewi
Indonesian Journal of Global Health Research Vol 5 No 4 (2023): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v5i4.2200

Abstract

Diabetes Mellitus (DM) is a chronic disease that occurs due to the failure of the pancreas to produce sufficient insulin. Diabetes Mellitus not only causes death but is also a major cause of blindness, heart disease, and kidney failure. Examination of HbA1c levels in the blood serves to help diagnose diabetes mellitus. A high HbA1c value is used as a measure of the development of diabetes complications. Mean Platelet Volume (MPV) is a marker of platelet function and activation. Platelets in DM patients will synthesize thromboxane in larger quantities than normal platelets. So it is important to check Hba1c and MPV to detect complications. This study aims to determine the relationship between HbA1c and MPV values ​​in type-2 DM patients. The type of research used is observational analysis with a cross-sectional approach. The sampling technique was quota sampling technique, then the results were analyzed using the Spearman correlation test. The results of the Spearman's Rho correlation test in this study showed a significant p-value of 0.100, which means that there is no significant relationship between HbA1c levels and MPV values ​​in patients with type 2 DM.
DNA Quality Testing of Isolated Stored Giemsa Blood Smear Samples using Geneius™ Micro gDNA Extraction Kit as An Alternative Sample for Malaria Molecular Examination Saroh, Dewi; Haryatmi, Dwi; Widodo, Rendyansah Agung
Indonesian Journal of Global Health Research Vol 7 No 1 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i1.4844

Abstract

The gold standard for malaria examination is microscopic examination. However, microscopic examination has many shortcomings, namely the difficulty of distinguishing between Plasmodium sp. species when mixed infections occur. Other malaria examinations for screening can be done with RDT. However, it also has shortcomings, namely the minimum number of parasites that can be detected is 100 parasites/ul. Molecular examination is the most sensitive and accurate examination in the diagnosis of malaria. The initial stage of molecular examination is the DNA isolation stage. This DNA isolation is very important in determining the success of molecular examination. Isolation of Plasmodium sp. DNA using blood smear samples is still rarely done and there is no standard procedure for isolating DNA from blood smears. The purpose of this study was to determine whether stored Giemsa blood smears can be used as samples in DNA isolation in malaria cases. The method used in isolation is using the Geneius™ Micro gDNA Kit. The results of the study were that stored Giemsa blood smear samples of Plasmodium vivax for 3 months could be isolated using the Geneius™ Micro gDNA Kit and could be used as DNA templates for amplification with target gene primers.