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All Journal Jurnal Medik Veteriner Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary

Hariadi, Mas'ud

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Author-ID : 1533729

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Articles

Detection of Fertility Levels of Female Bawean Deer (Axis kuhlii) Based on Fecal Steroid Metabolic Rahmawati, Mitha Ardila; Hariadi, Mas'ud; Restiadi, Tjuk Imam; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Srianto, Pudji
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.84-90

The aim of the study was to detect the fertility level of females Bawean deer (Axis kuhlii) through fecal steroid metabolic which were tested using Enzyme-Linked Immunosorbent Assay (ELISA). The study used five females Bawean deer in Taman Flora Surabaya, which were marked using R1, R2, R3, R4, and R5 necklaces. The feces of each deer was collected on the 1st day, 6th day, 11th day, 16th day, and 21st day. The samples which were collected then extracted using the freeze dry method. Samples were tested using ELISA. The results of the measurement of the levels of fecal steroid metabolic in five Bawean deers showed that three Bawean deer (R1, R4, and R5) were in the luteal or pregnant phase as indicated by the increased of progesterone from 1st day to 21st day. While the other two Bawean deer (R2 and R3) were in the follicular or estrous phase as indicated by the decreased of level of faecal steroid metabolic on 11th day for deer R2 and 16th day for deer R3. The results showed that the levels of the fecal steroid metabolic can be used to determine the follicular phase and luteal phase and the pregnancy status of female Bawean deer.

POTENSI KANDUNGAN ANTIOKSIDAN RUMPUT KEBAR (Biophytum Petersianum Klotzsch) TERHADAP APOPTOSIS DAN GAMBARAN HISTOPATOLOGIS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPAR 2,3,7,8-Tetrachlorodibenzo-p-dioxin Dewita, Dewita; Widjiati, Widjiati; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Hariadi, Mas'ud; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19986

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic compound in the dioxin group. This compound is a pollutant for the environment and very harmful to human health and enter the body through the mucous membranes in the mouth and the respiratory tract and can be transmitted through the placenta and lactation. The aim of this study was to know the antioxidantpotency of to resolve reproduction disturbance caused by TCDD exposure.Thirty Balb/C male mice were divided into five different groups, the negative control group, a positive control group exposed to TCDD at a dose of 7 Î¼g/kg BW, P1 group of groups exposed to TCDD doses of 7 Î¼g / kg BW and given Biophytum petersianum extract 0.05mg/gBB/day, group P2 group exposed to TCDD doses of 7Î¼g/kgBW and given 0.080mg/gBB/day, and group P3 were exposed to TCDD dose of 7Î¼g/kgBB and given the extract of Biophytum petersianum 0.135mg/gBB/day during day 2 to day 55. On the 56th day the mice were sacrificed and apoptotic examination and spermatogenic cell histopathological features were performed on the testis. The results showed that: P2 (0,433 ± 0,497; p<0,05) and P3 (0,200 ± 0,000 p<0,05) groups were the most effective group in decreasing spermatogenic cell apoptosis compared to positive control group (2,933 ± 1,5832 p<0,05). The Johnsen score result showed that P2 (9,400 ± 0,420) and P3 (9,800 ± 0,253) groups improving the histopathologic picture of spermatogenic cells in seminiferous tubules compared to positive control group (7,20 ± 0,400) p<0,05. Conclusion of this study were Biophytum petersianum is effective to solve reproduction disturbances caused by exposure of TCDD and the P3 group is the most effective group.

CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERANAKAN LIMOUSIN DAN SAPI PERANAKAN ONGOLE HASIL IB DI KECAMATAN PILANGKENCENG KABUPATEN MADIUN Bhaskara, Alfiutha Propha; Suprayogi, Tri Wahyu; Darsono, Roesno; Srianto, Pudji; Hariadi, Mas'ud; Sardjito, Trilas
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The study was conducted to know conception rate and service per conception of Limousin Cross breed and Ongole Cross Breed in Pilangkenceng, Madiun. Data were collected for the periode of Oktober 2016 to February 2017 from inseminators in Pilangkenceng district. The data were taken by primary and secondary. Primary data retrieval were done by direct observation, which includes several variables, where the variables include: the identity of the breeder, feeding and drinking. The secondary data were obtained by recording the farmer's artificial insemination card. All data was analyzed to find a percentage of conception rate (CR) and find number of service per conception (S/C). Then S/C were analyzed with T-test adn CR were analyzed with chi-square test. The results of this research showed that the service per conception's average of limousin cross breed and ongole cross breed is 1,61 and 1,72. The average of conception rate limousin cross breed and ongole cross breed is 53% % and 59 %. The result showed no significant difference (p>0,05) on CR and S/C between limousin cross breed and ongole cross breed. The conclusion of this research showed that service per conception and conception rate of ongole cross breed in Pilengkenceng Madiun is better than limousin cross breed but there's no significant difference (p>0,05).

UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN Andriyanti, Silvia Rani; Hariadi, Mas'ud; Darsono, Roesno; Srianto, Pudji; wurlina, wurlina; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a 73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.

ANALISIS FAKTOR YANG MEMPENGARUHI KEJADIAN MASTITIS SUBKLINIS DAN KLINIS PADA SAPI PERAH (STUDI KASUS DI KOPERASI AGRIBISNIS DANA MULYA KECAMATAN PACET, KABUPATEN MOJOKERTO) Nisa, Hefi Choirun; Purnomo S., Bambang; Damayanti L., Tita; Hariadi, Mas'ud; Sidik, Romziah; Harijani, Nenny
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The study was conducted to know the factor that potentially affect the incidence of subclinical and clinical mastitis in dairy cows in the area Agribusiness Cooperative Dana Mulya Pacet, Mojokerto. Data retrieval were done by examining the incidence of subclinical and clinical mastitis in 100 lactation dairy cows. Milk samples were taken from milking each quarter of dairy cows in the afternoon, then tested using CMT reagent (California Mastitis Test). In addition data was obtained from farmers through structured questionnaires. The data was processed and presented in tabular form and analyzed statistically by the method of regression trees. The conclusion of this research showed that dairy cows in the area of Agribusiness Cooperative Dana Mulya Pacet, Mojokerto affected by subclinical mastitis reached 76% and clinical mastitis 11%. This was influenced by the milking sequence factor of the nipple which was not distinguish between sick and healthy nipples, the lack of sanitation before milking, and the location of livestock disposal is too close to the cowshed.

HUBUNGAN ANTARA UMUR DENGAN CALVING INTERVAL, DAYS OPEN, DAN SERVICE PER CONSEPTION SAPIFRIESIAN HOLSTEIN (FH) Ananda, Hanifah Muslimah; Wurlina, Wurlina; Hidajati, Nove; Hariadi, Mas'ud; Samik, Abdul; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research was to know the relationship between age with calving inteval (CI), days open (DO), and service per conception (S/C) in Friesian Holstein dairy cattle (FH). The research was started on Desember 2017 to January 2018 in PT. Greenfields Indonesia partnerships, KecamatanWagir Kabupaten Malang. The materials of the search used were 100 heads of dairy cattle which had at least two times of parturition. The method used in this research was a survey and data collection. The data were obtained from the records of reproduction. Data analysis was multiple linear regression using SPSS software 21.0 version. The results showed that the values of CI (434,9±58,9 days, 449,4±66,2 days, and 431,8±59,2 days), DO (218,9±58,7 days, 218,9±58,7 days, dan 217,6±54,1 days), dan S/C (3,2±1,8 times, 4,3±1,9 times, 2,6±1,1 times) for the ages of 4, 5, 6. The relationship between age with CI and DO were not significant (P>0,05), but the relationship between age with S/C was significant (P<0,05). The value of S/C increased on dairy cattle at age of 4 to 5 years and started to decreased at the age of 6 years.

PENGARUH EKSTRAK DELIMA MERAH(Punicagranatum L.) TERHADAP PENINGKATAN PERSENTASE KAPASITASI DAN REAKSI AKROSOM SPERMATOZOA TIKUS PUTIH (Rattusnorvegicus) Satrio, Baktiar Budi; Hariadi, Mas'ud; Wahjuni, Retno Sri; Utomo, Budi; Sudjarwo, Sri Agus; Damayanti L, Tita
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this research is to know the effect of pomegranate (Punicagranatum L.) on the improvement of spermatozoa quality that is increase of percentage of capacitation and acrosome reaction in white rat (Rattusnorvegicus). This research used 20 healthy white rat (Rattusnorvegicus) with average age 2-3 months old, 200-300 g. Research design using completely randomized design (CRD) with four treatments and 5 replications. Rats were randomly divided into 4 treatment groups. Rat in the negative control group not treated just kept alone, solvent control group were given CMC Na 0,5% and is exposed to heat, the treatment groups 1, 2, and 3 induced pomegranate extract standardized to 40% ellagic acid at a dose of 75mg / kg, 150mg / kg and 300mg /kg perorally with doses 1 ml/rat/day gastric for along 14 days and exposed to heat. Data were analyzed using One Way Analysis of Variant (ANOVA) followed by Duncan (p<0,05). The result showed that there was significant effect from therapy on the quality of rats spermatozoa especially to capacitation and acrosome reaction. The treatment from treatment 2 group showed the best result in maintaining the quality of rats spermatozoa with 27.6± 3.04 values for capacitation and 6,80 ± 1,30 for acrosome reactions. Extract of pomegranate (Punicagranatum L.) standardized to 40% ellagic acid has a significant change in improve the quality of spermatozoa, namely an increase in the percentage of capacitation and acrosome reactions in white rats (Rattusnorvegicus) exposed to heat.

Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling Azura, Sarah; Ratnani, Hermin; Susilowati, Suherni; Hariadi, Mas'ud; Samik, Abdul; Soepranianondo, Koesnoto
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.

Serum Progesterone profile of pregnant Ettawa crossbred does at day-0, -21, -42, -63 and -84 Bellani, Ayu Aprodhita Cintya; Ismudiono, Ismudiono; Hariadi, Mas'ud; Lokapirnasari, Widya Paramita
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.53-58

This study aimed to determine the progesterone concentrations of Ettawa crossbred does at day-0, -21, -42, -63, and -84 after mating. Five Ettawa crossbred does were injected with PGF2α twice with an interval of 11 days for estrous synchronization followed by natural mating on day-3 after the second PGF2α injection. Blood was collected through the jugular vein for the measurement of serum progesterone concentrations using ELISA, whereas pregnancy was diagnosed at 90 days after mating by abdominal palpation. The results showed that all does were on estrus onthethird day after the second PGF2α, three of them diagnosed to be pregnant following mating. The progesterone concentration at day-0 was 1.52 ± 0.33 ng/mL, increased (p <0.05) to 7.08 ± 1.02 and 5.50 ± 2.82 ng/mL at day-21 and day-42, and increased (p <0.05) again to 12.01 ± 5.30 and 12.04 ± 0.30 ng/mL at day 63 and day 84, respectively. There was no significant difference in progesterone concentrations between day 21 and day 42 and between day 63 and day 84. It could be concluded that there was a biphasic increase of progesterone concentrations of the pregnant Ettawa crossbred does, i.e. from day-0 to day-21/day-42 and from day-21/day-42 to day-63/day-84.

Correlation of parity and age to services per conception, conception rate, and gestation length in Holstein Friesian cross cows Ulfah, Norma Amalia; Samik, Abdul; Hariadi, Mas'ud; Suprayogi, Tri Wahyu; Hidanah, Sri
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.9-14

Small farmers dominate dairy farming in Indonesia, and the average productivity is low. The contribution of domestic production only reaches about 23% of the total national milk consumption needs as a source of human nutrition. This study was aimed to identify the relationship between cow's parity and age to services per conception (S/C), conception rate (CR), and gestation length (GL) of Holstein Friesian (HF) cross dairy cows. Data of this study consisted of primary data obtained from farmers interview, and secondary data from the Cooperative Unit of Argopuro village, Krucil, Probolinggo. Sample size was 200 cows, which was 10% of the total population. Data were analyzed for correlation and linear regression using the Statistic Package and Service Solution (SPSS) software version 20 (IBM Corp., USA) at a 95% confidence level. The average S/C, CR, and GL were 2.55 ± 1.57, 21%, and 278.19 ± 11.92 days, respectively. There was no significant correlation (p >0.05) between the parity with S/C, CR, and GL and the age with CR and GL. The only significant correlation (p <0.05) was between the age and S/C. Regression of S/C based on age followed the equation S/C = 0.104 + 0.407*age, with a correlation coefficient and determination coefficient of 0.733 and 0.538, respectively. It could be concluded that S/C could be predicted based on the age of the cows.

Co-Authors Abdul Samik, Abdul Ananda, Hanifah Muslimah Andriyanti, Silvia Rani Azura, Sarah Bellani, Ayu Aprodhita Cintya Bhaskara, Alfiutha Propha Budi Utomo Damayanti L, Tita Damayanti L., Tita Darsono, Roesno Dewita Dewita Hani Plumeriastuti Harijani, Nenny Hermin Ratnani Hidajati, Nove Hidanah, Sri Ismudiono Ismudiono, Ismudiono Nisa, Hefi Choirun Pudji Srianto Purnomo S., Bambang Rahmawati, Mitha Ardila Rimayanti Rimayanti Romziah Sidik, Romziah Satrio, Baktiar Budi Soepranianondo, Koesnoto Suherni Susilowati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Ulfah, Norma Amalia Wahjuni, Retno Sri Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wurlina, W

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