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All Journal Media Veteriner Jurnal Veteriner Jurnal Biodjati Jurnal Medik Veteriner Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Jurnal Pengabdian Masyarakat Charitas Jurnal Agro Veteriner

Pudji Srianto

Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 3693346

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary

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Therapy of Human Chorionic Gonadotropin (hCG) Hormone in Dairy Cattles that Have Repeat Breeder on Pregnancy Rate in Tulungagung, East Java, Indonesia Muhammad Aulia Rahman; Sri Pantja Madyawati; Oky Setyo Widodo; Pudji Srianto; Trilas Sardjito; Rimayanti
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16979

This study aimed to prove that giving hCG injection at the time of artificial insemination and five days afterartificial insemination in dairy cows with repeat breeder can cause pregnancy. This study employed 30 dairycows with repeat breeder. All samples of dairy cows were divided into two treatments, P1 using hCG at adose of 100 IU given intramuscularly at the time of artificial insemination and P2 using hCG at a dose of100 IU intramuscularly given five days after artificial insemination. The results were analyzed using the Chi-Square test. Analysis using Chi-Square showed that all treatments caused pregnancy (100%). In summary,hCG injection at the time of artificial insemination and five days after artificial insemination in dairy cowsexperiencing repeat breeding can cause pregnancy.

Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfe Imam Mustofa; Laba Mahaputra; Pudji Srianto; Suzanita Utama
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar

Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfered contralaterally seven days post AI. The percentage of pregnancy in groups which had received 100 and 150 mg MPA was 37.5%r, and in group received 200 mg MPA and control was 55.6%, and 60%, (P>0.05), respectively. Calving percentages of the 100, 150, 200 mg MPA and the control groups were 25,37.5,55.6 and 60% respectively (P>0.05). All birth gave single calve.

Antibiotic Resistance in Escherichia coli Isolated from Feces of Bali Cattle With Reproductive Disorders Kholik Kholik; Muhammad Munawaroh; Muhammad Rama Imam Saputra; Rahmawati Rahmawati; Pudji Srianto
Jurnal Biodjati Vol 6, No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.13925

Antimicrobial resistance (AMR) has become a global issue in animal, human and environmental health. The AMR profile of Escherichia coli reflects the use of antibiotics in production animals. The purpose of this study was to determine the antibiotic resistance of Escherichia coli bacteria isolated from the feces of female Bali cattle with reproductive disorders. Feces samples were taken purposively using a swab on 4 rectums from 7 Bali cattle with reproductive disorders in June 2021 in Lando Village, East Lombok Regency. Escherichia coli samples were isolated on Eosin Methylene Blue Agar media and identified by biochemical tests. An antibiotic resistance test against Escherichia coli was carried out by the disk diffusion method. The antibiotics used in the test were Penicillin G 10 U, Oxytetracycline 30 g, Gentamicin 10 g, and Tetracycline 30 g, and Cefotaxime 30 g. The results of planting on Eosin Methylene Blue Agar media obtained 4 Escherichia coli which were successfully isolated from 4 samples of Bali cattle feces. Data on the level of Escherichia coli susceptibility level to various antibiotics were analyzed using the chi-square test. The results of the susceptibility test to antibiotics showed that 4 samples of Escherichia coli (100%) were resistant to Penicillin G, (25%) were resistant to Oxytetracycline, (25%) were resistant to Cefotaxime, and (100%) samples of Escherichia coli were sensitive to Gentamicin and Tetracycline. The chi-square test on the level of Escherichia coli susceptibility to various antibiotics was significant with p˂ 0.05 (p-value = 0.012). The results of this study have shown that Escherichia coli from Bali cattle feces experience multidrug resistance which later on might have an impact on human health and the environment.

Peningkatan Ekspresi Vascular Endothel Growth Factor dan Mitogen Activating Protein Kinase Plasenta Tikus yang Dipapar Carbon Black ((INCREASING EXPRESSION ON VASCULAR ENDOTHELIAL GROWTH FACTOR AND MITOGEN ACTIVATING PROTEIN KINASE IN PLACENTA RATS EXPOSE Viski Fitri Hendrawan; Widjiati Widjiati; Suherni Susilowati; Pudji Srianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Carbon black is one of the components of particulate matter (PM), known as cytotoxic, causing inflammationin the respiratory and cardiovascular system, and able to pass through the placental barrier, which may affect the fetus. The effects caused by pollutants depend on the intensity and time length of the exposure.The objective of this study is to identify Vascular Endothelial Growth Factor (VEGF) and Mitogen Activating Protein Kinase (MAPK) as an indicator of maternal placental inflammation in pregnant rats (Rattus norvegicus) exposed to carbon black with immunohistochemical technique. This study was a laboratory experiment with three treatment groups and seven replications using pregnant female white rats (Rattus norvegicus). Pregnant female rats were exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 during pregnancy of day 6 up to day 11. The next phase was the observation and examination of the expressions of VEGF and MAPK by immunohistochemical staining toward placenta of the white rats exposed to carbon black. The result of the study showed an increase in the expression of VEGF in the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 per inhale during the mid-period (6-11) of pregnancy. In addition, there was also an increasein the expression of MAPK on the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3and 1064 mg/m3 per inhale during the mid-period (day 6-11) of pregnancy. In conclusion, the expression of VEGF and MAPK are increased along with the increased dosage of carbon black and age of gestation. ABSTRAK Carbon black merupakan salah satu komponen partikulat matter (PM) yang diketahui bersifat sitotoksik serta menyebabkan terjadinya inflamasi pada sistem pernapasan dan kardiovaskular serta dapat melewati barier plasenta fetus sehingga dapat memengaruhi fetus. Efek yang ditimbulkan oleh polutan tergantung dari besarnya paparan dan lama waktu paparan. Penelitian ini bertujuan untuk mengidentifikasi Vascular Endothel Growth Factor (VEGF) dan Mitogen Activating Protein Kinase (MAPK) sebagai indikator imflamasi pada plasenta maternal tikus (Rattus norvegicus) yang dipapar carbon black dengan teknik imunohistokimia. Penelitian ini dilakukan dengan tiga kelompok perlakuan dan tujuh kali ulangan yang menggunakan tikus betina bunting. Tikus betina bunting dipapar dengan carbon black dosis 523 mg/m3 dan 1064 mg/m3 selama kebuntingan hari ke-6 sampai 11. Tahap berikutnya adalah pengamatan dan pemeriksaan ekspresi VEGF dan MAPK dengan pewarnaan imunohistokimia terhadap plasenta tikus putih yang telah dipapar dengan carbon black. Hasil penelitian adalah terjadi peningkatan ekspresi VEGF pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan (6-11) kebuntingan. Selain itu terjadi peningkatan ekspresi MAPK pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan(6-11) kebuntingan. Simpulan penelitian ini adalah ekspresi VEGF dan MAPK meningkat seiring dengan peningkatan dosis carbon black dan umur kebuntingan.

THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Dhesy Kartikasari; Sri Mulyati; Suzanita Utama; Pudji srianto; Widjiati Widjiati; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.

Correlation of Body Condition Score and Parity in Dairy Cows that Experienced Repeated Breeding in Sendang, Tulungagung Pristian Devon Martyas Wahyuningrum; Pudji Srianto; Budiarto; Tita Damayanti Lestari; Herry Agoes Hermadi; Erma Safitri
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.57623

Repeat breeding is a syndrome which affected the reproduction and production efficacy of dairy cattle. Dairy cow is one of an animals which produce milk. This syndrome may cause high economic losses in dairy cattle farm. This research was conducted from February to March 2023. The aim of this research was to determine the correlation of body condition score and parity of repeat breeding on the dairy cow at Tani Wilis Cooperation, Sendang, Tulungagung. A total of 46 dairy cows that contributed to the repeat breeding were used in this study. Sampling was done by purposive sampling with the following condition the date AI and the date of parturition. This research was conducted in a survei with primary and secondary data collection. Primary data retrieval is done by interviewing farmers, body condition score of each dairy cow, and direct observation. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by inseminator. Data were analyzed using correlate statistics followed by Spearmann Test. The result of the research indicated that no significant difference (P>0,05) in body condition score and parity.

Persentase Motilitas dan Viabilitas Spermatozoa Kambing Sapera dalam Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Before Freezing Masyitoh, Herlina; Suprayogi, Tri Wahyu; Praja, Ratih Novita; Srianto, Pudji; Madyawati, Sri Pantja; Saputro, Amung Logam
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui perbedaan persentase motilitas dan viabilitas semen kambing Sapera terhadap evaluasi semen segar dan before freezing dengan penambahan pengencer tris kuning telur dan susu skim kuning telur. Metode penelitian ini adalah pengambilan semen, evaluasi semen segar (pemeriksaan makroskopis dan mikroskopis), pengenceran semen, gliserolisasi, ekuilibrasi dan evaluasi before freezing. Penelitian ini terdiri dari P0, P1 dan P2, masing-masing perlakuan terdiri dari sembilan ulangan. P0 adalah semen segar kambing Sapera, P1 adalah semen kambing Sapera+pengencer tris kuning telur dan P2 adalah semen kambing Sapera+pengencer susu skim kuning telur. Data yang diperoleh dengan Uji T2 sampel dependent, membandingkan P0 dan P1 serta P0 dan P2. Data menunjukkan perbedaan sangat nyata (p<0,01) pada perlakuan P0 dan P1 persentase motilitas 76,11%; 65,56% dan viabilitas 82,33%;72,22%. P0 dan P2 persentase motilitas 76,11%;61,11% dan viabilitas 82,33%;67,78%. Hasil tersebut menunjukkan bahwa penelitian persentase motilitas dan viabilitas spermatozoa kambing Sapera mengalami penurunan dari semen segar pada tris kuning telur dan susu skim kuning telur before freezing.

Efektifitas Daya Pisah Electric Separating Sperm (ESS) terhadap Spermatozoa Kromosom X dan Y pada Kambing Sapera Lailiyah, Fahlevi; Srianto, Pudji; Saputro, Amung Logam; Madyawati, Sri Pantja; Agustono, Bodhi; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui efektifitas pemisahan alat Electric Separating Sperm (ESS) terhadap spermatozoa kromosom X dan Y pada sisi anoda dan katoda dengan variabel meliputi presentase viabilitas, motilitas, abnormalitas dan morfometri spermatozoa. Metode pengambilan semen menggunakan vagina buatan, kemudian dilakukan pemeriksaan makroskopis dan mikroskopis, apabila menunjukkan hasil yang baik dilanjutkan dengan pengenceran semen dengan Tris Kuning Telur dan dilakukan pemisahan. Terdapat tiga perlakuan pada penelitian ini yaitu P1 (3 menit), P2 (7 menit), P3 (10 menit) dengan enam kali pengulangan pada sisi anoda dan katoda. Data pada tiap variabel (viabilitas, motilitas dan abnormalitas) diperiksa menggunakan analisis statistik ANOVA dan untuk melihat adanya pemisahan spermatozoa kromosom X dan Y dilakukan pengukuran terhadap kepala menggunakan morfometri. Hasil pemisahan tertinggi yaitu pada P3 dengan perbandingan pemisahan X : Y sejumlah 65,00:35,00 pada sisi katoda, kemudian pada sisi anoda persentase perbandingan X : Y yaitu 59,00:41,00. Hasil kualitas spermatozoa setelah pemisahahn menunjukkan presentase viabilitas terbaik terdapat pada P1 yaitu 75,00a±2,00 pada sisi anoda dan 76,50a±2,17 pada sisi katoda. Motilitas spermatozoa P1 menunjukkan motilitas progresif spermatozoa dengan rataan 60,83a±2,04 pada anoda dan 61,67a±2,58 pada sisi katoda. Presentase abnormalitas tidak menunjukkan perbedaan yang signifikan antar perlakuan.

Pengukuran Kadar Hormon Progesteron dan Deteksi Birahi pada Sapi Perah yang Disinkronisasi dengan CIDR (Controlled Internal Drug Release) Widodo, Oky Setyo; Srianto, Pudji; Wulandari, Shelly
Jurnal Medik Veteriner Vol. 2 No. 2 (2019): October
Publisher : Universitas Airlangga

Deteksi birahi dan program perkawinan merupakan cara yang tepat untuk dapat mengoptimalkan performa reproduksi pada sapi perah. Rancangan penelitian yang dilakukan dalam penelitian ini adalah pretest-postest design. Terdiri dari empat macam perlakuan yaitu P1C1, P1C2, P2C1, dan P2C2, masing-masing perlakuan diulang sebanyak empat kali. Faktor yang diujikan adalah faktor P: usia korpus luteum 15 hari (P1) dan 10 hari (P2), faktor C: pencabutan atau pelepasan implan progesterone pada hari ke-9 (C1) dan pada hari ke-13(C2). Hasil yang diperoleh dengan uji statistik menunjukkan bahwa tidak terdapat interaksi antara perbedaan usia korpus luteum dengan perbedaan waktu pencabutan atau pelepasan implan controlled internal drug release (CIDR) (p>0.05). Dari total sampel 16 ekor sapi perah, 13 sampel menunjukkan tanda birahi tiga hari setelah implan controlled internal drug release (CIDR) dilepaskan. Persentase total dari keseluruhan sampel yang menunjukkan tanda birahi adalah 81.25%.

Detection of Fertility Levels of Female Bawean Deer (Axis kuhlii) Based on Fecal Steroid Metabolic Rahmawati, Mitha Ardila; Hariadi, Mas'ud; Restiadi, Tjuk Imam; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Srianto, Pudji
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.84-90

The aim of the study was to detect the fertility level of females Bawean deer (Axis kuhlii) through fecal steroid metabolic which were tested using Enzyme-Linked Immunosorbent Assay (ELISA). The study used five females Bawean deer in Taman Flora Surabaya, which were marked using R1, R2, R3, R4, and R5 necklaces. The feces of each deer was collected on the 1st day, 6th day, 11th day, 16th day, and 21st day. The samples which were collected then extracted using the freeze dry method. Samples were tested using ELISA. The results of the measurement of the levels of fecal steroid metabolic in five Bawean deers showed that three Bawean deer (R1, R4, and R5) were in the luteal or pregnant phase as indicated by the increased of progesterone from 1st day to 21st day. While the other two Bawean deer (R2 and R3) were in the follicular or estrous phase as indicated by the decreased of level of faecal steroid metabolic on 11th day for deer R2 and 16th day for deer R3. The results showed that the levels of the fecal steroid metabolic can be used to determine the follicular phase and luteal phase and the pregnancy status of female Bawean deer.

Co-Authors . Kholik Abdul Samik, Abdul Abdullah Hasib Aditya Irawan Aftabuddin Rz, Muhammad Agustono, Bodhi Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Aldin Akbar Rahmatullah Ali Saifudin Andriyanti, Silvia Rani Azwani, Nabila Bambang Poernomo Berliana, Erprinanda Galuh Bhaskara, Alfiutha Propha Boedi Setiawan Brillianti, Febby Fairy BUDI UTOMO Budi Utomo Budiarto Budiarto Budiarto Cahya P., Aghid Cindy Ercha Aulia Putri Dadik Rahardjo Dadik Rahardjo, Dadik Dadik Raharjo Dadik Raharjo Daniel Leonardo Darsono, Roesno Deskayanti, Asih Dhesy Kartikasari Dian Ayu Permatasari Dian Ayu Permatasari Dikman, Dicky Mohammad Dimas Kunto Satrio Djoko Legowo Dwi Puspa Sukma Viranda Emy Koestanti Sabdoningrum Endang Suprihati Erlangga, Made Bagus Erma Safitri Erma Safitri ERMA SAFITRI Fajar Ramadhan Subiyantoro Febriano, Muhammad Galang Gandul Atik Yuliani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Imam Mustofa IMAM MUSTOFA Indah Norma Triana, Indah Norma Ira Sari Yudaniayanti Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kartikasari, Dhesy Kurniawan, Muhammad ‘Ahdi Laba Mahaputra Lailiyah, Fahlevi Luqman, Epy Muhammad Masyitoh, Herlina Mohammad Auzaie Afandi Muhammad Aulia Rahman Muhammad Munawaroh Muhammad Rama Imam Saputra Muhammad Yunus M’ Izi Kumala Lazuardi Sultoni Nidom, Chairul Anwar Nove Hidajati Nurhusien Yimer Nuurin Ajrin Karim Praja, Ratih Novita Prastiya, Ragil Angga Pratama, Hanif Sabekti Pristian Devon Martyas Wahyuningrum Rahmawati Rahmawati Rahmawati, Mitha Ardila Rimayanti Rimayanti Rimayanti Rimayanti Rimayanti Ristiani, Widya Aulia Rizki, Andaru Rochmah Kurnijasanti Saputro, Amung Logam Seagames Waluyo, Seagames Setiawan Koesdarto Setyadi, Arda Wahyu Sintya Kumalasari Wibowo Sintya Kumalasari Wibowo Soeharsono Soeharsono Soeharsono Soeharsono Sofie Adi, Keren Jayanti Sri Hidanah Sri Mulyati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Subagio, Muharam Suherni Susilowati Sukma, Puspa Permata Sunarso, Agus Supriyadi Supriyadi Suwarno Suwarno Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Viski Fitri Hendrawan Warsito, Sunaryo Hadi Widjiati w Widjiati Widjiati Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Wulandari, Shelly Wurlina, W Yustinasari, Lita Rakhma Zahrorona, Amira Halimah

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