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All Journal Jurnal Ilmu Ternak Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Current Biomedicine Jurnal Agro Veteriner
Tita Damayanti Lestari
Department Of Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Pengujian Anti Protein Produksi Blastosis (Anti-PAG) melalui Metode Dot Blot (Evaluatin Of Anti PAG From Blastosis through Dot Blot Method) Tita Damayanti Lestari
Jurnal Ilmu Ternak Vol 11, No 1 (2011)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v11i1.407

Abstract

Penelitian  pengujian  reaksi antiprotein dan protein produksi blastosis dilakukan dengan tujuan mengetahui apakah  terjadi saling mengenali antara anti-PAG dan protein PAG yang terdapat dalam serum sapi bunting. Pengujian dilakukan dengan menggunakan metode Dot Blot. Uji dilakukan terhadap serum sapi bunting   umur 15, 20, 25 dan 30 hari, masing-masing dari empat ekor dengan dosis pengenceran anti-PAG adalah 1/100, 1/200 dan 1/400. Hasil reaksi berupa warna ungu dikonversikan kedalam angka menggunakan metode NIH Image Version 1.62. Data dianalisis menggunakan Rancangan Acak Lengkap Pola Tersarang, dilanjutkan dengan uji jarak berganda Duncan untuk mengetahui pengaruh serum dalam dosis. Uji statistik pada hasil reaksi Dot Blot menunjukkan perbedaan yang  signifikan antara serum sapi dara bunting 15,20,25 dan 30 hari dalam dosis pengenceran 1/100, 1/200 dan 1/400. PAG pada serum sapi dara bunting 15 hari mampu dikenali oleh anti-PAG pada pengenceran 1/400. Serum sapi dara bunting umur 30 hari memiliki nilai paling sensitif pada semua dosis pengenceran, disusul kemudian dengan serum sapi umur kebuntingan 25, 20 dan 15 hari. Kata Kunci : Anti-PAG, PAG, Dot Blot, serum  sapi bunting
Correlation of Body Condition Score and Parity in Dairy Cows that Experienced Repeated Breeding in Sendang, Tulungagung Pristian Devon Martyas Wahyuningrum; Pudji Srianto; Budiarto; Tita Damayanti Lestari; Herry Agoes Hermadi; Erma Safitri
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.57623

Abstract

Repeat breeding is a syndrome which affected the reproduction and production efficacy of dairy cattle. Dairy cow is one of an animals which produce milk. This syndrome may cause high economic losses in dairy cattle farm. This research was conducted from February to March 2023. The aim of this research was to determine the correlation of body condition score and parity of repeat breeding on the dairy cow at Tani Wilis Cooperation, Sendang, Tulungagung. A total of 46 dairy cows that contributed to the repeat breeding were used in this study. Sampling was done by purposive sampling with the following condition the date AI and the date of parturition. This research was conducted in a survei with primary and secondary data collection. Primary data retrieval is done by interviewing farmers, body condition score of each dairy cow, and direct observation. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by inseminator. Data were analyzed using correlate statistics followed by Spearmann Test. The result of the research indicated that no significant difference (P>0,05) in body condition score and parity.
Total plate count dalam isi telur ayam ras di pasar tradisional Kecamatan Waru, Kabupaten Sidoarjo Budiarto, Budiarto; Kirana, Sabila Citra; Lestari, Tita Damayanti; Budiarto; Puspitasari, Yulianna; Mutamsari Witaningrum, Adiana; Permatasari, Dian Ayu
Current Biomedicine Vol. 1 No. 2 (2023): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.2.86-94

Abstract

Background: Microbial contamination in consumed chicken eggs can endanger human health. Bacteria can grow and develop in food so that it can reduce the food quality. Determination of food quality can be done by various methods, one of which is Total Plate Count (TPC). Objective: The aim of this study was to determine the level of bacterial contamination in chicken eggs sold at traditional markets in Waru District, Sidoarjo Regency. Methods: Thirty samples were collected from two traditional markets, Waru Market and Wadung Asri Market. This study used the pour plate method of TPC test. Egg contents were diluted and then inoculated in Nutrient Agar. The media inoculated with the sample suspension was incubated at 37°C for 24 hours. The growing bacterial colonies were counted and analyzed using the Standard Plate Count. This study's data are presented descriptively and compared with the Indonesian National Standard (SNI) 7388-2009 maximum requirement of contaminant bacteria in chicken eggs is 1×105 CFU/mL. Results: The results showed that the averages TPC in two traditional markets were 2.1×104 CFU/mL in Waru Market and 3.4×103 CFU/mL in Wadung Asri Market. The average TPC still meets the maximum limit of microbial contamination listed in SNI 7388-2009. Conclusion: The average values of TPC in both markets are not the same, but the qualities of chicken eggs sold in Waru Market and Wadung Asri Market are good and suitable for consumption.
Detection of Fertility Levels of Female Bawean Deer (Axis kuhlii) Based on Fecal Steroid Metabolic Rahmawati, Mitha Ardila; Hariadi, Mas'ud; Restiadi, Tjuk Imam; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Srianto, Pudji
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.84-90

Abstract

The aim of the study was to detect the fertility level of females Bawean deer (Axis kuhlii) through fecal steroid metabolic which were tested using Enzyme-Linked Immunosorbent Assay (ELISA). The study used five females Bawean deer in Taman Flora Surabaya, which were marked using R1, R2, R3, R4, and R5 necklaces. The feces of each deer was collected on the 1st day, 6th day, 11th day, 16th day, and 21st day. The samples which were collected then extracted using the freeze dry method. Samples were tested using ELISA. The results of the measurement of the levels of fecal steroid metabolic  in five Bawean deers showed that three Bawean deer (R1, R4, and R5) were in the luteal or pregnant phase as indicated by the increased of progesterone from 1st day to 21st day. While the other two Bawean deer (R2 and R3) were in the follicular or estrous phase as indicated by the decreased of level of faecal steroid metabolic  on 11th day for deer R2 and 16th day for deer R3. The results showed that the levels of the fecal steroid metabolic can be used to determine the follicular phase and luteal phase and the pregnancy status of female Bawean deer.
Profile of Progesterone Levels After Administration of Gonadotropins in Dairy Cattle with Ovarian Hypofunction Berliana, Erprinanda Galuh; Utama, Suzanita; Wurlina, Wurlina; Srianto, Pudji; Lestari, Tita Damayanti; Ratnani, Hermin
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.1-5

Abstract

This study aimed to evaluate the profile of progesterone in dairy cattle with ovarian hypofunction. A total of 10 cows in this study were evaluated three times in the collection phase, i.e. (F1) when the cow was diagnosed with ovarian hypofunction, (F2) when the cow was in heat and (F3) 21 days after artificial insemination to detect pregnancy. Samples in the form of blood serum were then analyzed using ELISA. As a result, the average time of heat in ovarian hypofunction cows was 7,4 days. Progesterone levels in F1 were 1,027 ng/ml, 2,774 ng/ml, 1,476 ng/ml, 2,256 ng/ml, 1,258 ng/ml, 1,758 ng/ml, 2,393 ng/ml, 0,592 ng/ml, 0,755 ng /ml, 1,876 ng/ml, respectively. Progesterone levels in F2 were 0,671 ng/ml, 0,517 ng/ml, 0,763 ng/ml, 0,598 ng/ml, 0,615 ng/ml, 0,537 ng/ml, 0,726 ng/ml, 0,643 ng/ml, 0,593 ng/ml, 0,975ng/ml, respectively. Progesterone levels in F3 were 15,642 ng/ml, 4,215 ng/ml, 17,327 ng/ml, 20,721 ng/ml, 5,796 ng/ml, 17,214 ng/ml, 15,815 ng/ml, 16,745 ng/ml, 4,632 ng /ml, 18,281 ng/ml, respectively. The pregnancy rate in hypofunctional cows treated with PG-600 in this study was 70%.
Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences Amrullah, Muhammad Fajar; Utomo, Budi; Utama, Suzanita; Suprayogi, Tri Wahyu; Lestari, Tita Damayanti; Restiadi, Tjuk Imam; Belgania, Ristaqul Husna
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.125-131

Abstract

The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.
Effect of Probiotic Administration of Bacillus subtilis and Bacillus coagulans Isolate on Growth Performance in Broiler Chicken Faiqoh, Berliana Elok; Lamid, Mirni; Damayanti, Ratna; Chusniati, Sri; Al Arif, Mohammad Anam; Warsito, Sunaryo Hadi; Lestari, Tita Damayanti; Raharjo, Hartanto Mulyo; Rehman, Saifur; Hussain, Muhammad Asif
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.99-106

Abstract

This study aimed to determine the effect of probiotic administration of Bacillus subtilis and Bacillus coagulans isolate in improving feed intake, and body weight, and feed conversion rate (FCR) in broiler chicken. A total of 24 broiler chickens divided into four treatments i.e. (P0) was a group without probiotics, (P1), (P2), and (P3) were experimental treatment groups consist of probiotic 2 mL/day, 4 mL/day, and 6 mL/day per orally. In results, feed intake, body weight, and FCR values in all treatment groups (P1, P2, P3) were significantly different (p < 0.05) from (P0) group. Meanwhile, there was no significant difference (p > 0.05) between P1 and P3 group. It can be concluded that the effect of B. subtilis and B. coagulans as probiotics with a dose of 4 mL/day had a noticeable impact on feed intake, body weight, and decreased FCR.
Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Sidoarjo, Indonesia Sari, Aulia Puspita; Koesdarto, Setiawan; Lestari, Tita Damayanti; Hastutiek, Poedji; Yunus, Muchammad; Herupradoto, Eduardus Bimo Aksono; Khairullah, Aswin Rafif; Ayuti, Siti Rani; Hasib, Abdullah; Aryaloka, Suhita; Moses, Ikechukwu Benjamin
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.370-381

Abstract

The problem most often encountered in fish farming is disease which can cause a decrease in fish production levels. Parasites cause one of the infectious diseases found in fish farming. The presence of parasites can cause a decrease in the quality of the growth of fish production. The purpose of this study was to identify the prevalence of ectoparasites on Nile tilapia in Sidoarjo, Indonesia. Ninety-six samples from two ponds were examined under the microscope to find out the presence of ectoparasite that exists in the outside body part of the fish. Samples were collected from skin, fins, tail, and gills using native methods and observed under a microscope in the Division of Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. The results of this study showed 86 samples positively infected by ectoparasite and 10 showed negative. The ectoparasites found in this study were Trichodina sp., Chilodonella sp., Ichthyophthirius multifillis, Gyrodactylus sp., and Dactylogyrus sp. The highest prevalence is Trichodina sp. and Dactylogyrus sp. The number of fish infested by ectoparasite is 89.56%, this is classified in the usual category. The highest prevalence occurs in infestations of Trichodina sp. i.e.72.91% which were contained in the 70 samples. While the lowest prevalence was in Gyrodactylus sp. by 8.32% in 8 fish samples. Double infections from two different ponds were 70% within 33 samples which were included in the usual category.
Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Charles, Vanessa Ann; Lestari, Tita Damayanti; Legowo, Djoko; Ismudiono, Ismudiono; Hidajati, Nove; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
The Correlation between the Weight of Ovary, Number of Follicles, and Quality of Oocytes of Culling Female Bovine in Slaughterhouse Chandramohan, Koshini; Utama, Suzanita; Lestari, Tita Damayanti; Rimayanti; Safitri, Erma; Hestianah, Eka Pramyrtha; Mulyati, Sri; Damayanti, Ratna; Khairullah, Aswin Rafif; Hasib, Abdullah
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.206-220

Abstract

The purpose of this research was to determine the correlation between the weight of the ovary, the number of follicles, and the quality of the oocytes from the culling female bovine   obtained from the slaughterhouse. The variation may affect the potential of the ovary to produce follicles. Paired ovaries were obtained from female bovines.  Follicle numbers were recorded and divided into three size categories (small: 3 mm, medium: < 3 mm-8 mm, and large: > 8 mm). Oocytes were aspirated and the number of oocytes was recorded and graded into four categories (grades A, B, C, and D). There was a positive correlation between the weight of the ovary and the number of follicles, which is 0.560 with the regression equation y = 3.52 + 0.501 x. There was a positive correlation between the number of follicles and the number of oocytes, which is 0.546 with the regression equation y =2.48 + 1.204 x. There was a positive correlation between the number of oocytes and the grade of oocytes, which is 0.520, with the regression equation y = 0.93 + 0.800 x. There was no correlation between the weight of the ovary and oocyte grade A, which is 0.013.
Co-Authors Abdul Samik, Abdul Adiana Mutamsari Witaningrum Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Amrullah, Muhammad Fajar Aryaloka, Suhita Ayuti, Siti Rani Belgania, Ristaqul Husna Berliana, Erprinanda Galuh Boedi Setiawan Budi Utomo Budiarto Budiarto Budiarto Chandramohan, Koshini Charles, Vanessa Ann Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo Dewi, Aprilia Kurnia Dikman, Dicky Mohammad Dimas Kunto Satrio Djoko Legowo Eka Pramyrtha Hestianah, Eka Pramyrtha ERMA SAFITRI Erma Safitri Faiqoh, Berliana Elok Farah Ghifara Hafidha Frida Aulya Arningdiah Gandul Atik Yuliani Gesa Cantya Primatara Hariadi, Mas'ud Hasib, Abdullah Heni Puspitasari Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri Hussain, Muhammad Asif Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Khairullah, Aswin Rafif Kirana, Sabila Citra Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mafruchati, Maslichah Meydilasari, Nanda Irsa Mirni Lamid Mohammad Sofi’ul Anam Moses, Ikechukwu Benjamin Mufasirin Muhammad Al-Syafiq bin Abdul Halim Mustofa Helmi Effendi M’ Izi Kumala Lazuardi Sultoni Nove Hidajati Nuurin Ajrin Karim Pasang, Gabriel Sampe Permatasari, Dian Ayu Poedji Hastutiek Pristian Devon Martyas Wahyuningrum Pudji Srianto Putri, Natalie Lovena Pramono Raharjo, Dadik Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmawati, Mitha Ardila Rahmi Sugihartuti Ratna Damayanti Rehman, Saifur Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Saputra, Rakan Mahiid Sari, Aulia Puspita Seagames Waluyo, Seagames Setiawan Koesdarto Shabira, Zahra Sharveihn Sathindren Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharsono Suharsono Suherni Susilowati Supriyadi Supriyadi Supriyadi Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tjuk Imam Restiadi Tri Wahyu Suprayogi Triagil, Annisa Lita Trilas Sardjito Tsaniya Atikahani Shabirah Viski Fitri Hendrawan Wahyuni, Retno Sri Warsito, Sunaryo Hadi Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Yulianna Puspitasari Yunus, Muchammad Zulfanisa, Rizky Asrin