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All Journal Terampil : Jurnal Pendidikan dan Pembelajaran Dasar Majalah Patologi Indonesia Journal Mathematics Education Sigma [JMES]
Triana, Novi
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Education Health Professions Mathematics Public Health Other

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Hypertrophy Determination of H9c2 Cardiomyoblast Cell Line Using Wright-Giemsa Staining: An Experience in Developing an Acceptable and Easy-to-handle In-vitro Protocol Fadhillah, Muhamad Rizqy; Arozal, Wawaimuli; Wibowo, Heri; Bustami, Arleni; Sukmawati, Dewi; Kusmardi, Kusmardi; Triana, Novi; Khatimah, Nurul Gusti
Majalah Patologi Indonesia Vol. 33 No. 2 (2024): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v33i2.682

Abstract

Introduction Cell-size area (CSA) becomes the standard parameter routinely tested in vitro for cardiac hypertrophy studies. Thus, staining is an essential tool for this purpose. As reported in a previous study, immunofluorescence staining is an established method for CSA. However, because it is expensive and requires a specialized microscope, e.g., immunofluorescence or confocal microscope, it is not applicable in a laboratory with limited equipment. Wright-Giemsa staining is a standard procedure in hematology laboratories and is inexpensive and convenient. Here, we shared our experience developing a CSA determination protocol using Wright-Giemsa in H9c2 cardiomyoblast. Methods The viability tests were performed on H9c2 to determine the effective dosage of angiotensin II and Irbesartan (standard drug). The H9c2 were divided into three groups: the control group (without either angiotensin II or irbesartan), the negative control (with angiotensin II), and the positive control (with angiotensin II and Irbesartan), triplicate for each group. The cells then are acclimatized overnight, serum-starved for one day, and incubated with angiotensin and irbesartan for 48 hours. Lastly, Wright-Giemsa was observed using a light microscope in three fields. The CSA was determined by three independent observers blindly, statistically different if the p<0.05 using ANOVA ways or Kruskal-Wallis. Results After the H9c2 induced by angiotensin-II 1 μM and Irbesartan 1μM, we found the CSA significantly differed among each group (p<0,0001). The negative control has a higher median and interquartile range (IQR) CSA (10.78 (6.79) um2) compared to the control group (median (IQR) 7.27 (4.91) um2) and positive control (median (IQR) 7.849(5.31) um2). Conclusion It can be concluded that the Wright-Giemsa might help determine the CSA for in-vitro hypertrophic studies.
MEDIA PEMBELAJARAN BERBASIS PERMAINAN DALAM MATA PELAJARAN MATEMATIKA UNTUK MENINGKATKAN MOTIVASI BELAJAR SISWA Yonanda, Pramestry; Susanti, Ely; Triana, Novi; Ramadhona, Nuril Fajria
Journal Mathematics Education Sigma [JMES] Vol 6, No 1 (2025): JOURNAL MATHEMATICS EDUCATION SIGMA
Publisher : Journal Mathematics Education Sigma [JMES]

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/jmes.v6i1.20794

Abstract

Tujuan penelitian ini adalah untuk meningkatkan motivasi belajar siswa pada mata pelajaran matematika melalui media pembelajaran berbasis permainan. Penelitian ini merupakan penelitian tindakan kelas yang terdiri dari beberapa tahap yaitu perencanaan, pelaksanaan, observasi, dan evaluasi. Subjek penelitiannya adalah siswa kelas III D di SD Negeri 130 Palembang dengan jumlah 26 siswa yang terdiri dari 12 anak perempuan dan 14 anak laki-laki. Instrumen yang digunakan pada penelitian ini adalah observasi dan wawancara yang dianalisis secara deskriptif kualitatif. Pada siklus I diperoleh 49,6% siswa yang sudah termotivasi untuk belajar dan pada siklus II diperoleh 87,2% siswa yang termotivasi untuk belajar. Dapat disimpulkan bahwa hasil implementasi media pembelajaran berbasis permainan dalam mata pelajaran matematika berhasil karena terdapat peningkatan yang signifikan pada motivasi belajar siswa.
Improving Student Learning Motivation Through Differentiated Learning in Pancasila and Citizenship Subjects in Grade 1 of Elementary School Sari, Pemi Purnama; Susanti, Ely; Avida, Avida; Yonanda, Pramestry; Triana, Novi
TERAMPIL: Jurnal Pendidikan dan Pembelajaran Dasar Vol 11 No 2 (2024): TERAMPIL
Publisher : Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/terampil.v11i2.23695

Abstract

The purpose of this study was to determine the increase in learning motivation of first grade students through differentiated learning in the subject of Pancasila and Citizenship Education. The research method used was classroom action research (CAR) with the Kemmis MC Taggart research model consisting of 2 cycles, which included four stages, namely planning, implementation, observation, and reflection. The subjects of this study were first grade students at SD Negeri 130 Palembang. The object of research in this study was students' learning motivation for the subject of Pancasila and Citizenship Education. Data collection techniques used observation sheets and interviews. The data obtained in this study were analyzed descriptively quantitatively and descriptively qualitatively. The results of cycle I showed that 40.6% of students already had learning motivation in the good and very good categories so that action was needed in cycle II because it had not reached 50% of motivated students. In cycle II, 81.2% of students already had learning motivation in the good and very good categories. Based on the results of cycles I and II, it can be concluded that there was an increase in student learning motivation through differentiated learning.
Co-Authors Arleni Bustami Avida, Avida Dewi Sukmawati Ely Susanti Fadhillah, Muhamad Rizqy Heri Wibowo Khatimah, Nurul Gusti Kusmardi Kusmardi Ramadhona, Nuril Fajria Sari, Pemi Purnama Wawaimuli Arozal Yonanda, Pramestry