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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Transmisi: Jurnal Ilmiah Teknik Elektro Ijtimaiya : Journal of Social Science and Teaching Indonesian Journal of Industrial Engineering & Management Jurnal Teknik Terapan (J-TETA) jabdimasunipem International Journal of Technology, Food and Agriculture SEJAGAT : Jurnal Pengabdian Masyarakat
Rofi'i, Ahmad
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BZLF1 Expression of EBV is correlated with PARP1 Regulation on Nasopharyngeal Carcinoma Tissues fajri, wahyu nur laili; Rofi'i, Ahmad; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Nasopharyngeal carcinomas (NPC) is a cancer that arises in the epithelial tissue that covers the inside of the nasopharyngeal mucosa and nasopharynx. Infected Epstein Barr Virus (EBV) cell in a latent infection associated with the expression of nine latent proteins. Latent Membrane Protein 1 (LMP1) is one of latent proteins, and mayor EBV oncoprotein, with functions including virus growth, and to activate BamHI-Z Leftward Reading Frame 1 (BZLF1)-EBV, which can inhibit p53 to induce apoptotic resistance, metastasis, and immune modulation. The body will respond to the expansion of EBV infection with activation of Poly(ADP-ribose)Polymerase-1 (PARP1). The objective of study is to observe the expression of BZLF1 and determine PARP1 regulation in nasopharyngeal tissues. NPC-T2, NPC-T3 and polyp tissues slides are from Ulin Hospital, Banjarmasin. To characterize the necrotic cells such as pyknosis, karyorrhexsis, and karyolysis, histological slides were stained by HE that the necrotic cells measured by using a BX-53 microscope (Olympus) with CellSens Standard software. Tissues slides were stained by using immunofluorohistochemistry with EBV-BZLF1 antibody-Mouse anti-EBV monoclonal antibody against Goat anti-mouse IgG-FITC and anti-PARP1 antibody (MC-10) against Goat anti-mouse IgG labeled Rhodamin. The expression intensities were measured by Confocal Laser Scanning Microscope (Olympus). The percentage number of necrotic cells and BZLF1 and PARP1 expression intensity were analyzed using SPSS 16.0 by one-way ANOVA test with α = 0.05, beside that we use correlate and regression analyze. The research showed that the amount of karryorhexis higher than pyknosis and karyolysis in both tissues. BZLF1 expression 1.79 INT/sel (in polyp), 2.76 INT/sel (NPC Type 2) and 4.36 INT/sel (NPC Type 3), PARP1 expression 2.25 INT/sel (in polyp), 3.31 INT/sel (NPC Type 2), dan 5.93 INT/sel (NPC Type 3).The high of intensity of expression BZLF1 induced the increasing of PARP1 expression, but not correlated with percentage of necrotic cell. Interestingly, in all tissues (NPC-T1, NPC-T2 and polyp) is increased number of karyolysis cells properly, but not in pyknosis and karryorrhexis cells. The conclusion of this study indicated that the BZLF1 induce PARP1 to repair DNA damage against EBV infection.Keywords: BZLF1, CLSM, EBV, nasopharyngeal carcinoma, PARP1.
Co-Authors Aditya Wahyu Pratama Afara, Afida Nur Afianah, Nuzula Afriansyah, Faisal Lutfi Afriansyah, Faisal Luthfi Alfiansyah, Faisal Luthfi Asom, Khotimul Crismono, Prima Cristi Dicky Adi Tyagita Dwianto, Sihmaulana fajri, wahyu nur laili Fanani, Nurul Zainal Farzanggi, Faza Dildari fatchiyah . Hakim, Alief Lukman Izza, Istna Ismatul Kautsar, Syamsiar Kindi, Muhammad Koesacep, HAP Muthohar, Habib Nafsiyah, Haniatun Nari, Mochamad Irwan Nari, Mochammad Irwan Nari, Muhammad Irwan Pitasari, Mely Agustin Reni Purnomo, Fendik Eko Putri, Salsabila Liandra Rohman, Ardianto Syaifur Tunjung Genarsih, Tunjung Wahyu Pratama, Aditya Winadi, Aditya Wahyu Winardi, Aditya Wahyu