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Potensi Antimikroba Ekstrak Etanol Daun Gatal (Urticastrum decumanum (Roxb.) Kuntze) Terhadap Pertumbuhan Staphylococcus aureus dan Candida albicans Yanti, Subar -; Meianti, Durkas Sabat Dwi; Manalu, Rosario Trijuliamos
Sainstech Farma: Jurnal Ilmu Kefarmasian Vol 15 No 2 (2022): Sainstech Farma: Jurnal Ilmu Kefarmasian
Publisher : LPPM, INSTITUT SAINS DAN TEKNOLOGI NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37277/sfj.v15i2.1272

Daun gatal (Urticastrum decumanum) merupakan tanaman asli Papua yang banyak digunakan sebagai obat pereda nyeri. Penelitian bertujuan untuk mengetahui aktivitas antimikroba daun gatal terhadap Staphylococcus aureus dan Candida albicans. Daun gatal diambil dari Kabupaten Mimika, Kota Timika, Provinsi Papua, Indonesia. Daun gatal segar sebanyak 2 kg yang telah bersih dikeringkan dengan cahaya matahari tidak langsung. Serbuk daun gatal dimaserasi menggunakan etanol 96% dan dipekatkan pada suhu 40 oC hingga menjadi ekstrak kental. Penapisan fitokimia dilakukan terhadap senyawa alkaloid, tanin, flavonoid, saponin, dan steroid/triterpenoid. Ekstrak daun gatal dibuat empat konsentrasi yaitu 1,0; 1,5; 2,0, dan 2,5% dengan kontrol positif kloramfenikol untuk S. aureus dan ketokonazol untuk C. albicans, serta DMSO 10% sebagai kontrol negatif. Uji aktivitas antimikroba dilakukan dengan metode difusi cakram dan dilusi padat. Hasil penelitian menunjukkan bahwa ekstrak etanol daun gatal mengandung senyawa alkaloid, tanin, flavonoid, saponin, dan steroid/triterpenoid. Ekstrak etanol daun gatal pada konsentrasi 2,5% memiliki daya hambat paling tinggi terhadap S. aureus dengan diameter 12,7 mm dan 10,7 mm untuk C. albicans. Respon pertumbuhan S. aureus dan C. albicans dalam kategori lemah sampai sedang. Konsentrasi ekstrak etanol daun gatal yang ditetapkan sebagai konsentrasi hambat minimum (KHM) terhadap S. aureus dan C. albicans adalah 0,6%.

Maja Fruit (Crescentia cujete L.) Potential as a Laxative in Mice Teodhora, Teodhora; Manalu, Rosario Trijuliamos; Kusuma, Ika Maruya; Azizah, Shandy
Jurnal Kefarmasian Indonesia VOLUME 13, NUMBER 2, AUGUST 2023
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v13i2.6300

Constipation is a digestive disorder that anyone with symptoms of complex, uneven, and irregular bowel movements commonly experiences. This study aims to determine the laxative effect of maja extract (Crescentia cujete L.) on male white mice (Mus musculus) induced by loperamide. This fruit has been reported to be used as an analgesic, anti-inflammatory, and for digestive system disorders. The method used to test laxative activity is the intestinal transit method. Parameters observed included stool weight, defecation frequency, feces consistency, and not passage length in the intestine of mice. The extract was administered orally at a dose of 125 mg/kg BW, 250 mg/kg BW and 500 mg/kg BW; as a comparison, a negative control (CMC Na) and a positive control (docusate sodium) were used. Phytochemical screening of maja extract contains alkaloids, flavonoids, tannins, and saponins. The best results were obtained maja fruit can provide a therapeutic laxative effect through the molecular mechanism of secondary metabolites in loperamide-induced test animals.

Uji Aktivitas Antioksidan Ekstrak Etanol dan Fraksi Daun Gedi hijau (Abelmoschus manihot (L.) Medik) Dengan Metode DPPH (1,1-Difenil-2-Pikrilhidrazil) Manalu, Rosario Trijuliamos; Herdini; Danya, Fiki
Pharmaceutical Journal of Indonesia Vol. 8 No. 1 (2022)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2022.008.01.3

Daun gedi hijau memiliki potensi sebagai antioksidan, seperti halnya dengan daun gedi merah yang secara tradisional banyak digunakan sebagai pengobatan dan dibuktikan dengan penelitian memiliki aktivitas antioksidan. Tujuan penelitian ini untuk mengetahui aktivitas antioksidan dari ekstrak dan fraksi daun gedi hijau dengan metode DPPH. Bahan uji yang digunakan adalah serbuk kering daun gedi hijau yang diperoleh dari BALITTRO (Balai Penelitian Tanaman Rempah dan Obat) Kecamatan Bogor Tengah Kota Bogor Provinsi Jawa Barat. Pembuatan ekstrak dilakukan secara maserasi dengan etanol 96%, kemudian dievaporasi menjadi ekstrak kental dengan hasil rendemen sebesar 11,14%, selanjutnya difraksinasi cair-cair berturut-turut dengan pelarut n-heksan, etil asetat dan air. Pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-Difenil-2-Pikrilhidrazil) dengan vitamin C sebagai kontrol positif. Hasil uji aktivitas antioksidan pada ekstrak etanol awal, fraksi etil asetat dan fraksi air tergolong efektif, sedangkan fraksi n-heksan tergolong tidak efektif. Fraksi etil asetat menunjukkan aktivitas antioksidan paling tinggi dengan nilai IC50 233 bpj.

Uji Aktivitas Antioksidan Ekstrak Etanol dan Fraksi Daun Gedi hijau (Abelmoschus manihot (L.) Medik) Dengan Metode DPPH (1,1-Difenil-2-Pikrilhidrazil) Manalu, Rosario Trijuliamos; Herdini; Danya, Fiki
Pharmaceutical Journal of Indonesia Vol. 8 No. 1 (2022)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2022.008.01.3

Daun gedi hijau memiliki potensi sebagai antioksidan, seperti halnya dengan daun gedi merah yang secara tradisional banyak digunakan sebagai pengobatan dan dibuktikan dengan penelitian memiliki aktivitas antioksidan. Tujuan penelitian ini untuk mengetahui aktivitas antioksidan dari ekstrak dan fraksi daun gedi hijau dengan metode DPPH. Bahan uji yang digunakan adalah serbuk kering daun gedi hijau yang diperoleh dari BALITTRO (Balai Penelitian Tanaman Rempah dan Obat) Kecamatan Bogor Tengah Kota Bogor Provinsi Jawa Barat. Pembuatan ekstrak dilakukan secara maserasi dengan etanol 96%, kemudian dievaporasi menjadi ekstrak kental dengan hasil rendemen sebesar 11,14%, selanjutnya difraksinasi cair-cair berturut-turut dengan pelarut n-heksan, etil asetat dan air. Pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-Difenil-2-Pikrilhidrazil) dengan vitamin C sebagai kontrol positif. Hasil uji aktivitas antioksidan pada ekstrak etanol awal, fraksi etil asetat dan fraksi air tergolong efektif, sedangkan fraksi n-heksan tergolong tidak efektif. Fraksi etil asetat menunjukkan aktivitas antioksidan paling tinggi dengan nilai IC50 233 bpj.

The Relationship Between COMT and MAO-B Gene Polymorphisms with Levodopa in Parkinson’s Disease Patients; A Review Hasna, Vina Luthfiana; Kasasiah, Ahsanal; Manalu, Rosario Trijuliamos; Malau, Jekmal
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5228

Parkinson's disease is a degenerative nervous system disorder caused by the death of dopamine-producing cells in the substantia nigra. Dopaminergic treatment could alleviate motor symptoms for a period. One of the effective dopaminergic medications for symptomatic relief was Levodopa and dopamine agonists. Clinically, Levodopa was always combined with Dopa Decarboxylase (DDC) inhibitors, which redirected Levodopa metabolism towards the COMT pathway, increasing its bioavailability in the central nervous system. The purpose of this article was to investigate the relationship between COMT and MAO-B gene polymorphisms and Levodopa in Parkinson's disease patients, starting by gathering literature on the association between COMT and MAO-B polymorphisms and Levodopa in Parkinson's disease patients using various databases. Reviewed literature revealed that the most frequent polymorphism in the COMT gene was rs4680. Some polymorphisms significantly impacted the treatment of Parkinson's disease patients. However, despite efforts to identify genetic factors influencing the risk of side effects or treatment ineffectiveness, the role of pharmacogenetics in Parkinson's disease has not been fully explored and lacks consistent clinical recommendations. Further research was needed to tailor treatment to individual polymorphisms so that pharmacogenomic approaches could be applied more consistently

Antidiarrheal Potential of Maja (Crescentia cujete) Fruit Extract in Mice Teodhora, Teodhora; Manalu, Rosario Trijuliamos; Saputra, Richi Andika
Borneo Journal of Pharmacy Vol. 7 No. 4 (2024): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v7i4.4712

Diarrhea, characterized by frequent, loose stools, can be treated with various approaches. This study investigated the antidiarrheal properties of maja (Cresentia cujete) fruit extract, which contains secondary metabolites like tannins and flavonoids with astringent properties. The objective was to determine the optimal dose of C. cujete fruit extract for treating diarrhea in mice. An in vivo experimental design was employed, inducing diarrhea in male mice using oleum ricini. The study assessed stools' onset, duration, consistency, frequency, and weight. Cresentia cujete fruit extract was administered orally at 125, 250, and 500 mg/kg BW doses. A comparison was made with negative control (CMC-NA) and positive controls (loperamide and attapulgite). Data analysis involved ANOVA followed by the Tukey test. The findings revealed that 500 mg/kg BW of C. cujete fruit extract was the most effective dose for treating diarrhea in mice. These results suggest the potential of C. cujete fruit extract as a promising natural antidiarrheal agent.

Penentuan Aktivitas dan Potensi Senyawa Kalkon Daun Jambu Air (Eugenia aquea Burm f) Terhadap Sel T47D Kanker Payudara Secara In Silico Setyaningsih, Erwi Putri; Dari, Resti Wulan; Manalu, Rosario Trijuliamos
Sainstech Farma: Jurnal Ilmu Kefarmasian Vol. 18 No. 1 (2025): Sainstech Farma: Jurnal Ilmu Kefarmasian
Publisher : LPPM, INSTITUT SAINS DAN TEKNOLOGI NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37277/sfj.v18i1.2196

Breast cancer is a malignant tumor caused by excessive expression of one or all three estrogen (ER) and progesterone (PR) proteins and Human Epidermal Growth Factor Receptor (HER2). Current breast cancer treatment generally uses chemotherapy, radiotherapy, hormone therapy, and surgery. The increasing need for new drugs with high selectivity, low toxicity, and good metabolic stability against breast cancer has led many researchers to explore Indonesia's natural resources to obtain drugs that have anticancer activity. Chalcone is a secondary metabolite of the flavonoid group with pharmacological activity as an anticancer. Four chalcone compounds in jambu air leaves (Eugenia aquea Burm f) have met the requirement Breast cancer is a malignant tumor caused by excessive expression of one or all three estrogen (ER) and progesterone (PR) proteins and Human Epidermal Growth Factor Receptor (HER2). Current breast cancer treatment generally uses chemotherapy, radiotherapy, hormone therapy, and surgery. The increasing need for new drugs with high selectivity, low toxicity, and good metabolic stability against breast cancer has led many researchers to explore Indonesia's natural resources to obtain drugs that have anticancer activity. Chalcone is a secondary metabolite of the flavonoid group with pharmacological activity as an anticancer. Four chalcone compounds in jambu air leaves (Eugenia aquea Burm f) have met the requirements for drug similarity testing. In silico research is the initial step in designing new drug discoveries because it is relatively faster and can handle thousands of compounds. This study aimed to determine the activity and potential of jambu air (Eugenia aquea Burm f) leaf chalcone compounds in inhibiting the target protein of breast cancer T47D cells in silico and its ADMET prediction. The research method is in silico, with molecular docking techniques using the YASARA View, Marvinsketch, PubChem, PLANTS, and Discovery Studio 2021 applications. The results of the molecular docking analysis of the target protein, namely, Myrigalon-b have the potential to inhibit HER2 protein, Myrigalon-g has the potential to inhibit ERÎ±, and phloretin has the potential to inhibit ERÎ². The ADMET prediction results of the Myrigalon-g and phloretin compounds have the best values.

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