Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

P-Index

This Author published in this journals

All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Tanaman Industri dan Penyegar

Sudarsono, Sudarsono

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 1653128

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Industrial & Manufacturing Engineering

Published : 6 Documents Claim Missing Document

Claim Missing Document

Articles

Evaluasi Keseragaman Klonal Enam Klon Kakao Unggul Berdasarkan Marka SSR Sulistiyorini, Indah; Rubiyo, Rubiyo; Sudarsono, Sudarsono
Jurnal Tanaman Industri dan Penyegar Vol 5, No 3 (2018): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v5n3.2018.p135-144

Propagation of cacao plants is generally carried out vegetatively. Therefore, plants that are clonally propagated should be genetically uniform. Genetic uniformity in cacao clones is also very important information for germplasm conservation and in obtaining pure parental crosses. Evaluation of genetic uniformity can be seen through analysis using SSR markers. This study aimed to determine the genetic uniformity in six cacao clones using SSR markers. This experiment was conducted at IIBCRI Integrated Laboratory in Sukabumi and Plant Molecular Biology Laboratory, IPB Bogor, from September 2015 to December 2016.Â Six cacao clones used (TSH 858, TSH 908, ICS 13, PA 300, GC 7 and UIT) are from Kalitelepak experimental station ofÂ PTPN XII, Genteng District, Banyuwangi, East Java.Â Ten samples were taken randomly to represent cacao clones. DNA amplification was carried out using 12 SSR markers. The result showed that 12 SSR markers generated 45 alleles with the number of alleles per locus was 3-4 alleles. The polymorphic information content (PIC) ranges from 0.37â€“0.67, which are identied as very informative molecular analysis in identifying the genetical uniformity of the evaluated cacao population. Six SSR loci generated variant alleles within both the TSH 858 and UIT clones, indicating there are off-type plants in these two samples. Clonal uniformity were detected for samples of the GC 7, ICS 13, PA 300 and TSH 908 clones. On the other hand, 8.33% of evaluated samples within the TSH 858 and UIT clones were off-type plants.

Efektivitas Ekstrak Tumbuhan untuk Mengeliminasi Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Aprizal Zainal; Aswaldi Anwar; Satriyas Ilyas; , Sudarsono; , Giyanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Objectives of experiments were to evaluate (1) in vitro inhibitory effects of plant extracts on Clavibacter michiganensis subsp. michiganensis (Cmm), (2) inhibitory effects of plant extracts on Cmm infected tomato seeds, and (3) effectiveness of seed treatment plus plant extracts to eliminate Cmm. After evaluating 20 plant extracts, curcuma rhizome and betel vine leaf extract, cinnamon and clove oil were selected for further test. Tomato seeds were artificially inoculated with Cmm to obtain high level of infection. Part of the seeds were dipped in either suspension of selected extracts for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 °C and RH 60-70%) plus either of plant extracts oils, respectively. Elimination of Cmm level from infected seeds was observed at 10 days after treatments. Results of the experiment indicated curcuma extract, betel vine extract, cinnamon oil or clove oil showed in vitro inhibitory effects on Cmm. Moreover, dipping infected seeds in either 5% of curcuma, betel vine extract, or 0.5% of clove oil or matriconditioning plus these extracts oils were effective to eliminate Cmm from infected seeds. These treatments may potentially be used and developed commercially for eliminating seedborne Cmm on infected tomato seeds. Keywords: Bacterial cancer, seedborne-pathogen, seed-treatments

Evaluation of Somaclones Peanut Plants Regenerated from Repeat Cycles of In Vitro Selection Against Drought Stress A Farid Hemon; , Sudarsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this research was to evaluate the response of somaclonal peanut plants regenerated from repeated cycles of in vitro selection on medium containing 15% polyethylene glycol (PEG; w /v, corresponding to - 0.41 Mpa osmotic potential) against drought stress. The R2 generation of peanut plants were used in this experiment with cv "Kelinci" and "Singa" as control cultivars. Drought treatment was the plants irrigated with water to fi eld capacity (optimum condition) while other plants were grown under water deficit. Drought treatment was given at 16 to 85 days old peanut plants; after 85 days old, the plants were treated under optimum condition until plants were ready to harvest. Drought stress was measured using drought sensitivity index value (S) on scored parameters. Results of the experiment showed that peanut lines produced from repeated cycles of in vitro selection in medium containing 15% PEG were more tolerant to water deficit, had a better vegetative growth, a higher dry pod yield, and a lower dry pod yield reduction. This research demonstrated that repeated cycles of in vitro selection method was effective to produce drought tolerant peanut genotypes with a higher proline content than genotypes without in vitro selection. Keywords : somaclonal variation, polyethylene glycol, drought tolerance

Uji Inokulasi dan Respon Ketahanan 38 Genotipe To mat terhadap Clavibacter michiganensis subsp. michiganensis Aprizal Zainal; Aswaldi Anwar; Satriyas Ilyas; , Sudarsono; , Giyanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 2 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Identifi kasi ketahanan genotipe adalah langkah awal dalam pengembangan kultivar tahan terhadap serangan patogen. Tujuan penelitian ini adalah (i) mendapatkan cara inokulasi dengan jumlah dan konsentrasi inokulum Cmm yang efektif untuk mengevaluasi ketahanan tomat terhadap Cmm di rumah kaca, (ii) mendeterminasi reaksi ketahanan berbagai genotipe tomat akibat inokulasi Cmm. Percobaan ini menggunakan 38 genotipe tomat yang terdiri dari 7 genotipe tomat lokal, 15 genotipe tomat komersial, dan 16 genotipe koleksi Pusat Studi Pemuliaan Tanaman IPB Bogor (PSPT/IPB). Agen penyebab penyakit yang digunakan adalah 6 isolat Cmm hasil percobaan sebelumnya. Cara inokulasi Cmm yang efektif terhadap tomat cv. Marta (sangat rentan), uji reaksi ketahanan berbagai genotipe tomat terhadap Cmm telah dilakukan di rumah kaca. Kesimpulan dari penelitian ini adalah (i) inokulasi dengan menyuntikkan inokulum Cmm 5 µl konsentrasi 106 cfu/ml pada beberapa tempat di ketiak daun (daun pertama, daun tengah dan pucuk) merupakan cara yang paling efektif mengevaluasi ketahanan tomat terhadap Cmm, (ii) berbagai genotipe tomat yang diuji belum ada yang tahan teradap Cmm, genotipe tomat lokal ada yang agak rentan dan agak tahan.Kata kunci: kultivar tahan, suspensi bakteri, tomat

Perlakuan Invigorasi untuk Meningkatkan Mutu Fisiologis dan Kesehatan Benih Padi Hibrida Intani-2 Selama Penyimpanan Purnawati ,; Satriyas Ilyas; Sudarsono ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 3 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACT Storage condition and pathogen infection of seed can cause seed deteriorates faster. The rate of deterioration during storage could be slowed by seed invigoration, and pathogen infection could be elimited by application of natural pesticide. The objective of this research was to determine the effect of seed invigoration on seed physiological quality and health of Intani-2 hybrid rice seed during storage. All research activities were done at Laboratory of Quality Control and Plant Protection, PT. BISI International, Kediri, East Java. Split plot design was used in this experiment with 4 replications. Three seed lots of Intani-2 rice seed were used as main plot and 5 invigoration treatments were used as sub plot. Seed lot 1, 2 and 3 were harvested on 30 June 2012, 29 September 2012, and 2 November 2012, respectively. Invigoration treatments consisted of untreated, priming with ascorbic acid 40 ppm, osmoconditioning with KNO3 2%, osmoconditioning with PEG -0.2 MPa, and hydropriming. All invigoration treatments were added with clove oil 0.3%. Osmoconditioning with KNO3 2% + clove oil 0.3% was effective to maintain vigour index of seed lots 2 and 3 for up to 3 months storage. All seed invigoration treatments increased speed of germination before storage. Priming with ascorbic acid 40 ppm + clove oil 0.3% and osmoconditioning with PEG -0.2 MPa + clove oil 0.3% were effective to reduce the growth of Xanthomonas sp. on rice seeds monitored at 0, 2, and 3 months after storage.Keywords: ascorbic acid, clove oil, KNO3, osmoconditioning, PEG

Analisis Keragaman Morfologi dan Biokimia pada Anggrek Phalaenopsis (Orchidaceae) Aline Sisi Handini; Dewi Sukma; dan Sudarsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 1 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTPhalaenopsis orchid is one of famous genera in Orchidaceae family which have high economic value. Phalaenopsis breeding needs to be developed intensively to fulfill market demand for new varieties. The objective of this study was to obtain the information of morphological and biochemical diversity of a collection of Phalaenopsis orchid which will be useful for Phalaenopsis breeding. Plant materials used were 10 genotypes of Phalaenopsis with various petal colours of white, yellow, pink, deep pink, purple, or combination of yellow and brown. Morphological diversity of 10 genotypes were analyzed based on qualitative and quantitative characters following UPOV guidance for Phalaenopsis, while biochemical diversity was based on pigment content such as chlorophyl, anthocyanin, and carotene in plant roots, leaves and flower petals. The result of this research showed morphological diversity of 10 genotypes used was relatively high, with similarity of 55%. In that level of similarity, genotypes were grouped into two cluster. Biochemical analysis showed that most of genotypes were different for anthocyanin and carotenoid content for each plant organ. Genotype which showed high content of anthocyanin in leaves was Phal. hybrid ‘PH37’ while high content of carotene in leaves was Phal. hybrid ‘PIROUI. High content of anthocyanin in petals were found in Phal. hybrid ‘PH37’ and Phal. esmeralda, and high content of carotene were in Phal. cornucervi and Phal. cornucervi Red. Root with high anthocyanin content was found in Phal. hybrid ‘PIROU1’. There were significant correlation (0.975) for anthocyanin content between leaves and petals, petals and roots (0.953) on Phal.hybrid ‘PH37’, and between leaves and roots (0.874) on Phal. hybrid ‘PIROU1).Keywords: anthocyanin, carotene, chlorophyll, Phalaenopsis, similarity

Co-Authors , Giyanto . Giyanto A. Farid .Hemon Anwar, Aswaldi Aprizal Zainal Handini, Aline Sisi Purnawati , Rubiyo, Rubiyo Satriyas Ilyas Sukma, Dewi Sulistiyorini, Indah

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search