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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Pedagogi: Jurnal Ilmu Pendidikan Gontor AGROTECH Science Journal Kultivasi Jurnal Agro Industri Perkebunan Jurnal Pertanian Agros PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Inovasi Agroekoteknologi Tropika Lembab JURNAL CITRA WIDYA EDUKASI Agrica: Journal of Sustainable Dryland Agriculture Agro Sintesa : Jurnal Ilmu Budidaya Pertanian International Journal of Multi Discipline Science (IJ-MDS) Media Pertanian Jurnal Edukasi Pengabdian Masyarakat: EDUABDIMAS Jurnal Aplikasi Sains dan Teknologi : Agrisevika
Handini, Aline Sisi
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Respons pertumbuhan pakcoy terhadap asam humat dan Trichoderma dalam media tanam pelepah kelapa sawit Ratih Rahhutami; Aline Sisi Handini; Dwi Astutik
Kultivasi Vol 20, No 2 (2021): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v20i2.32601

Abstract

AbstrakPemanfaatan limbah organik dari perkebunan sebagai media tanam pakcoy (Brassica chinensis L.) diharapkan dapat ditingkatkan dengan penggunaan pupuk organik serta pupuk hayati. Penelitian disusun menggunakan Rancangan Acak Kelompok (RAK) pola faktorial. Faktor pertama adalah dosis asam humat meliputi 1, 3, dan 5 g. Faktor kedua adalah dosis Trichoderma sp., meliputi 50, 100, dan 150 mL. Data yang diperoleh kemudian dianalisis ragam pada taraf nyata 5%. apabila terdapat pengaruh nyata, dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa aplikasi asam humat dan Trichoderma sp. memiliki pengaruh mandiri dan tidak terdapat interaksi. Dosis asam humat 3 g per tanaman menghasilkan jumlah daun, panjang daun, tinggi tanaman, tinggi tanaman, bobot basah, dan bobot kering tanaman lebih tinggi dibanding dosis 1 dan 5 g. Perlakuan Trichoderma sp. dosis 50 mL per tanaman memiliki pengaruh lebih baik terhadap jumlah daun, panjang daun, tinggi tanaman, dan bobot basah tanaman.Kata Kunci: hortikultura, jamur, morfologi, senyawa organik Abstract The utilization of organic farm estate as pakcoy (Brassica chinensis L.) growing media may improved by using biofertilizer and organic fertilizer. The research used factorial randomized block design. First factor was humic acid dosage, which included 1, 3, and 5 g of humic acid. Second factor was Trichoderma sp. dosage, which included 50, 100, and 150 mL of Trichoderma sp. Data were analyzed using ANOVA at 5% level, then continued by DMRT test. The results showed that the application of humic acid and Trichoderma sp. had single effects and there was no interaction. The dosage of humic acid 3 g per plant had higher number of leaves, leaf length, plant height, wet weight, and dry weight than other dosages. The treatment of Trichoderma sp. at dosage of 50 mL per plant had a better effect on the number of leaves, leaf length, plant height, and plant wet weight.Keywords: fungi,  horticulture, morphology, organic compounds
Pengaruh Delignifikasi Termal Terhadap Substansi Dinding Sel Pada Limbah Bunga Jantan Kelapa Sawit Pasca Anthesis (The Influence of Thermal Delignification of Cell Substantials for The Waste of Oil Palm Mail Flowers Post Anthesis) Ratih Rahhutami; Aline Sisi Handini; Indriana Lestari
Jurnal Agro Industri Perkebunan Volume 8 No. 2, Oktober 2020
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v8i2.1377

Abstract

The post anthesis of male oil palm is one of the under utilized solid wastes,  with potential for processing into biofuel or bioethanol, as a welcome alternative to fossil fuels  The purpose of this study is to determine the best levels and the effect of several thermal delignification cycles on the levels of lignin, holocellulose, and cellulose.  This research used a one factor completely randomized design method with 3 treatments; one, two, and three cycle thermal delignification and data analyzed has a 5% variance. The results showed that the treatment of thermal delignification with several cycles had a significant effect on the levels of lignin, holocellulose, and cellulose. The best levels of lignin, holocellulose and cellulose were shown in the treatment of three cycles of thermal delignification.
Pengujian Viabilitas Benih Berbasis CDT (Controlled Deterioration Test) Pada Benih Melon (Cucumis melo L.) Aline Sisi Handini
AGRO SINTESA JURNAL ILMU BUDIDAYA PERTANIAN Vol 2, No 1 (2019): Agro Sintesa Jurnal Ilmu Budidaya Pertanian
Publisher : Lembaga Penelitian Universitas Swadaya Gunung Jati

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.211 KB) | DOI: 10.33603/jas.v2i1.2512

Abstract

Tujuan dari penelitia ini menggunakan uji vigor sebagai simulasi untuk mengetahui apakah benih dapat disimpan dalam jangka waktu yang lama dan memiliki vigor yang tinggi sampai perkecambahan di lapangan. Penelitian untuk menentukan vigor empat lot benih melon menggunakan controlled deterioration test (CDT) dengan suhu 45 ºC, kadar air benih 20%, 22%, 24% dan waktu deteriorasi 0, 24, 48, dan 72 jam. Rancangan yang digunakan adalah rancangan acak kelompok dengan dua faktor. Hasil analisis statistika menunjukkan bahwa terdapat interaksi antara varietas dengan kondisi CDT. CDT optimum yang dapat digunakan untuk uji vigor adalah 24%/24 jam dan 24%/48 jam dengan suhu 45 ºC.Kata kunci: benih melon, deteriorasi, vigor
The Utilization of Effective Microorganism 4 (EM4) on Growth of Oil Palm Seedling in Pre Nursery Dwi Astutik; Ratih Rahhutami; Aline Sisi Handini; Ahmad Sutopo
International Journal of Multi Discipline Science Vol 3, No 2 (2020): Volume 3 Number 2 August 2020
Publisher : STKIP Singkawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26737/ij-mds.v3i2.2117

Abstract

A planting medium that rich in nutrients is needed for oil palm, especially in the nursery phase. This study aimed to determine the effect of various doses of EM4 and to get the best dose of EM4 on the growth of oil palm seedlings in the pre-nursery. The research method used was a non-factorial completely randomized design with 4 treatments, namely 0 ml EM4 (A1), 4 ml EM4 (A2), 8 ml EM4 (A3), and 12 ml EM4 (A4). Each treatment was repeated 6 times so that there were 24 experimental units. The data obtained were analyzed for variance at the 5% level, if the effect was real, it was followed by the least significant difference (LSD) test. Based on the results of the study, it could be concluded that application of various EM4 doses had a significant effect on plant height (4-12 MST), the number of leaves (4-8 MST), trunk girth (4-12 MST), and shoot wet weight but did not significantly affect the number of leaves (12 MST), the volume of roots, length of roots, root wet weight and shoot dry weight, dry weight root, the best dose was shown at a dose of 12 ml EM4.
The Effectiveness of Humic Acid and Trichoderma sp. for Planting Media with Oil Palm Empty Bunches in Pakcoy (Brassica rapa L.) Aline Sisi Handini; Ratih Rahhutami; Dwi Astutik
International Journal of Multi Discipline Science Vol 3, No 2 (2020): Volume 3 Number 2 August 2020
Publisher : STKIP Singkawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26737/ij-mds.v3i2.2108

Abstract

Pakcoy is a vegetable commodity that has high economic value. This study aims to determine the effect of humic acid and Trichoderma sp. by utilizing empty fruit bunch (EFB) waste as a growing medium for Pakcoy growth. The research method used was a two-factor randomized block design. The first factor is humic acid (AH) with three levels: AH1 = 1 g, AH2 = 2 g, AH3 = 3 g. The second factor is Trichoderma sp. (T) with three levels; T1 = 50 ml, T2 = 100 ml, T3 = 150 ml. In this study it was concluded, the best treatment was found in the application of humic acid with a dose of 1 g on the parameters of observation of leaf length, leaf width, plant height, wet weight, and plant dry weight. The use of Trichoderma sp. in this study, does not affect all parameters observation. The results of the analysis of the planting medium showed that the pH was slightly acidic conditions 5.67 - 6.33. The organic matter content contained in the growing media ranged from 1.20 to 2.62%, and the total N-total contained in the growing medium ranged from 0.10 to 0.18. In-plant tissue analysis shows that the N-total found in plant tissue ranges from 4.62 - 5.23.
Teknik Hardening dan Aplikasi Paclobutrazol dalam Meningkatkan Vigor Planlet Anggrek Phalaenopsis amabilis Aline Sisi Handini
JURNAL CITRA WIDYA EDUKASI Vol 11 No 1 (2019): JCWE Edisi April 2019
Publisher : Politeknik Kelapa Sawit Citra Widya Edukasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1106.035 KB)

Abstract

Orchid is the one of largest flower species in the world, especially Phalaenopsis. This research was concern about post in vitro culture to improved planlets vigor. The objective of this study the Phalaenopsis orchid breeding process from tissue culture to enlargement to produce high-value crops. The result of observation showed that treatment H2P2 was the best. Statistic analysis showed that percentage survival rate 95%, lowest amount leaf, lowest height plant, lowest leaf width, lowest length leaf, and highest stomata density, founded in H2P2 treatment. So, can be used H2P2 treament as a recommendation for acclimatization for increasing the vigor planlets of Phalaenopsis amabilis.
Biodelignifikasi Bunga Jantan Kelapa Sawit Pasca Anthesis Menggunakan Trametes Versicolor Ratih Rahhutami; Aline Sisi Handini; Indriana Lestari
JURNAL CITRA WIDYA EDUKASI Vol 11 No 3 (2019): JCWE Edisi Desember 2019
Publisher : Politeknik Kelapa Sawit Citra Widya Edukasi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study were to determine the effect of biodelignification with Trametes versicolor and to get the best dosage of Trametes versicolor on the levels of lignin, holocellulose, hemicellulose and cellulose of oil palm male flowers post anthesis. This research was conducted at Laboratory of Chemistry Politeknik Kelapa Sawit Citra Widya Edukasi and Laboratory of Biochemistry Institut Pertanian Bogor in April – Oktober 2019. This study used a one factor completely randomized design method with 2 treatments: Trametes versicolor 40 ml (A1) and Trametes versicolor 60 ml (A2). Each treatments were three replications. The total unit of experiments were 6 unit. Data were analized with analysis of variance with α 5 %, if there was a significant treatment effect, the further analysis using LSD (Least Significant Differences). The results showed that biodelignification with Trametes versicolor did not significantly affect for all variabels and the dose of Trametes versicolor showed the same effectiveness for the levels of lignin, holocellulose, hemicellulose, and cellulose of oil palm male flowers post anthesis.
Analysis of Soil Chemical Properties, Production, and Nitrogen Content of Pakcoy in Various Growth Media and Trichoderma Dosages Ratih Rahhutami; Aline Sisi Handini; Dwi Astutik; Yeni Yeni
Gontor Agrotech Science Journal Vol. 9 No. 1 (2023): June 2023
Publisher : University of Darussalam Gontor, Ponorogo, East Java Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/agrotech.v9i1.9885

Abstract

The use of compost added by Trichoderma is one of improving the soil's chemical properties and can be used to increase the production of pakcoy plants. The purpose of this study was to determine the effectiveness of compost derived from various types of palm oil waste and the application of Trichoderma on soil chemical properties as well as production and nitrogen content in pakcoy plants. This study used an experimental method with parameters observing plant fresh weight. The research used two factors. The first factor was three types of palm oil waste, midrib leaf, oil palm empty bunches, and solid decanter. The second factor was the dose of Trichoderma, 50 ml, 100 ml, and 150 ml per plant. Planting media derived from solid decanter was able to intensification the production of pakcoy compared to midrib leaf and oil palm empty bunches.
Desain Primer Gen SMT 1 Terhadap Pertambahan Tinggi Batang Melambat pada Tanaman Kelapa Sawit (Elaeis gueneensis Jacq.) Berdasarkan Situs SNP Aline Sisi Handini; Jefryan Syaputra; Halida Adistya Putri
Media Pertanian Vol 8, No 2 (2023): Media Pertanian
Publisher : Program Studi Agroteknologi Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/mp.v8i2.8671

Abstract

Karakter pertambahan tinggi batang melambat merupakan salah satu tujuan dari pemuliaan tanaman kelapa sawit untuk optimalisasi umur ekonomis tanaman, mengurangi siklus replanting, dan mempermudah proses panen. Gen SMT 1 merupakan gen kunci didalam biosintesis hormon brasinosteroid yang berperan dalam pertumbuhan dan pertambahan batang tanaman. Teknik yang digunakan untuk mengidentifikasi gen yang memiliki karakter unggul tersebut adalah PCR (Polymerase Chain Reaction). Dalam proses PCR, desain primer merupakan hal yang sangat penting karena primer tersebut akan menentukan keberhasilan dalam proses amplifikasi PCR. Tujuan dari penelitian ini untuk mendapatkan kandidat primer yang akan digunakan dalam analisis keragaman genetik terkait pertambahan tinggi batang berdasarkan situs SNP. Data sekuen DNA kelapa sawit diperoleh pada laman NCBI. Pensejajaran sekuen DNA dilakukan pada sofware geneious prime untuk mengidentifikasi situs SNP. Desain primer dilakukan pada perangkat lunak WebSNAPER. Hasil penelitian menghasilkan dua kandidat pasang primer yang akan digunakan untuk melakukan analisis keragaman genetik terkait gen pengendali pertambahan tinggi batang melambat. Masing- masing primer terdiri dari primer reference dan alternate dan memiliki ukuran amplikon yang berbeda pada setiap pasang primer. The character of growth inhibitor stem is one of the goals of oil palm plant breeding to increase the economic life of the plant, reduce the replanting cycle and simplify the harvesting process. The SMT 1 gene is a key gene in the brasinosteroid hormone which plays a role in the growth and addition of plant stems. The technique used to identify genes with superior characters is PCR (Polymerase Chain Reaction). In the PCR process, the design of the primer is very important because the primer will determine the success of the PCR amplification process. The aim of this study was to obtain primer to be used in the analysis of genetic diversity related to stem height gain. Oil palm DNA sequence data were obtained in terms of NCBI. DNA sequence alignment was performed on geneious prime software to obtain SNP sites. Primer design was performed on WebSNAPER software. The results of the study obtained two candidate pairs of primers to be used for the analysis of genetic diversity related to the increase in the height of the stems of the stem. Each primer consists of reference and alternate primers and has a different amplicon size for each pair of primers.
Penghambatan Pencoklatan (Browning) pada Kultur In Vitro Kelapa Sawit menggunakan Beberapa Antioksidan: Inhibition of Browning in Oil Palm Tissue Culture using Several Antioxidants Halida Adistya Putri; Aline Sisi Handini; Sylvia Madusari; Josua Parulian Sitohang
Jurnal Ilmiah Inovasi Vol 23 No 3 (2023): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jii.v23i3.4018

Abstract

Teknik perbanyakan secara in vitro atau kultur jaringan mulai diaplikasikan untuk perbanyakan kelapa sawit, yang mana terbukti dapat menghasilkan tanaman kelapa sawit dalam jumlah banyak dengan waktu yang relatif lebih singkat.  Kendala utama saat fase inisiasi tanaman yaitu pencokelatan (browning) yang menyebabkan gangguan fisiologis tanaman sehingga terjadi kematian pada eksplan in vitro. Senyawa antioksidan merupakan senyawa yang dapat menghambat pencokelatan. Berbagai penelitian telah dilakukan untuk mencegah kemunculan browning pada kultur in vitro, namun setiap komoditas yang diuji memiliki senyawa antioksidan yang beragam untuk mendapatkan antioksidan terbaik yang dapat menghambat browning. Penelitian ini bertujuan untuk mendapat senyawa antioksidan terbaik untuk menghambat bahkan meniadakan terjadinya browning pada fase inisiasi kelapa sawit in vitro.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yaitu perlakuan zat antioksidan menggunakan tiga jenis zat antioksidan yaitu arang aktif (2.5 gL-1), Polyvinylpyrrolidone (PVP) (2.5 gL-1), dan asam akorbat 100 mgL-1). Data analisis ragam diuji lanjut menggunakan Duncan Multiple Range Test (DMRT) untuk data yang berbeda nyata pada taraf 5% dengan software STAR (Statistical Tool for Agriculture Research). Hasil penelitian menunjukkan antioksidan terbaik untuk menekan munculnya waktu browning dan intensitas browning adalah PVP sebesar 2.5 gL-1 pada tanaman kelapa sawit in vitro.
Co-Authors Ahmad Sutopo Arif Ravi Wibowo Arrufitasari, Putri Nur Careca Sepdihan Rahmat Hidayatullah Chakim, M. Ghufron Dewi Sukma Dwi Astutik Dwi Astutik Dwi Astutik Fikri, Galih El Indriana Lestari Jefryan Syaputra Josua Parulian Sitohang Kusnendi, Faizal Shofwan Priambodo, Oktanindita Purnomo Edi Sasongko Putri, Halida Adistya Rahhutami, Ratih Sibarani, Ebenezer Muaratama Sudarsono, dan Sutanto, Oentari Prilaningrum Sutopo, Ahmad Sylvia Madusari Yeni Yeni