Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

0.23

P-Index

This Author published in this journals

All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biodiversitas Journal of Biological Diversity

, Giyanto

Departemen Proteksi Tanaman Fakultas Pertanian Institut Pertanian Bogor Jl. Meranti Kampus IPB Darmaga, Bogor

Author-ID : 2431650

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

Published : 6 Documents Claim Missing Document

Claim Missing Document

Articles

KEANEKARAGAMAN ENZIM INVERTASE, PENGEMBANGAN STRAIN UNGGUL DAN TEKNOLOGI PRODUKSINYA Wilhelmus Terang Arga Sanjaya; . Giyanto; Rahayu Widyastuti; Dwi Andreas Santosa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Invertase Diversity, Novel Strain and Production Technology DevelopmentInvertase or Î²-fructofuranosidase (E.C.3.2.1.2.6) have been a valuable enzyme in food industry, so that research about enhancing invertase activity on an industrial scale has been reported massively. So far, the production of the invertase enzyme is highly dependent on the potential activity of the invertase enzyme derived from microorganisms. The development of enzyme production technology is also a concern in invertase research to obtain an efficient and inexpensive production system. So far, various developments in enzyme production technology and enzyme utilization have been carried out, including various innovations in immobilizing and increasing the stabilization of the invertase enzyme during the production process. The development of superior strains and enzyme production technology continues amid the discovery of the latest approaches such as genetic engineering, protein modification, and nanotechnology. This paper aims to discuss invertase variation from various organisms, its correlation to novel microbial strain development to increase invertase production and invertase enzyme production technologies development including immobilization technology and stabilization of invertase enzymes.Keywords: Î²-fructofuranosidase, ezyme production, fermentation, immobilitation, invertase geneABSTRAKInvertase atau Î²-fructofuranosidase (E.C.3.2.1.2.6) merupakan enzim yang sangat penting dalam dunia industri pangan sehingga berbagai optimasi aktivitas enzim untuk produksi dalam skala industri terus dilakukan. Produksi enzim invertase hingga sejauh ini sangat bergantung oleh potensi aktivitas enzim invertase yang diambil dari mikroorganisme. Selain itu, pengembangan teknologi produksi juga menjadi perhatian dalam pengembangan produksi dan pemanfaatan enzim untuk mendapatkan sistem produksi yang efisien dan murah. Sejauh ini berbagai pengembangan teknologi produksi enzim dan pemanfaatan enzim telah dilakukan, diantaranya dengan berbagai inovasi dalam melakukan imobilisasi dan peningkatan stabilisasi enzim invertase selama proses produksi. Pengembangan strain unggul dan teknologi produksi enzim terus dilakukan di tengah ditemukannya pendekatan-pendekatan terkini seperti rekayasa genentika, modifikasi protein, dan teknologi nano. Artikel ini bertujuan untuk membahas variasi enzim invertase dari berbagai organisme, korelasi pengembangan strain mikrobia unggul terhadap peningkatan produksi enzim invertase, dan perkembangan teknologi produksi enzim invertase meliputi teknologi imobilisasi dan stabilisasi enzim invertase.

Tingkat Keterjadian Penyakit Layu Stewart pada Benih dan Respon beberapa Varietas Jagung terhadap Infeksi Pantoea stewartii subsp. stewartii Haliatur Rahma; Meity S Sinaga; Memen Surahman; Giyanto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Disease incidence of Stewart’s wilt on the seed and response of several maize varieties to Pantoea stewartii subp. stewartii. Stewart’s wilt disease of maize is caused by Pantoea stewartii subsp. stewartii. This bacterium is seed-borne pathogens, when attacked maize caused yield lost 40-100%. The objective of this research was to detemine the incidence level of stewart’s wilt disease, growth of some varieties of maize and their response to stewart’s wilt pathogens Pantoea stewartii subsp. stewartii. The research was conducted in the Laboratory of Bacteriology and Greenhouse Cikabayan IPB from November 2011 to March 2012. In experiment I, nineteen samples of maize were used for symptom test in the maize seedling stage, using Randomized Block Design with three replications. Experiment II used a Randomized Block Design with 2 factors: maize varieties (8 hybrids varieties, 3 open pollinated varieties, and 7 sweet corn varieties) and bacteria isolates (BGR 2, BGR 4, BGR 28, BGR7 and PSM 27), with three replications. The results showed in experiment I, the incidence of stewart’s wilt disease ranged 2.00 – 15.33%, germination and vigor index of maize seed were 68.00 – 95.33% and 55.33 – 90.67% respectively. While in experiment II, hybrid and open pollinated of maize varieties were resistant to moderately susceptible while all sweet corn varieties were susceptible to infection of Pantoea stewartii subsp. stewartii.

Isolasi dan Seleksi Bakteri Endofit untuk Pengendalian Penyakit Darah pada Tanaman Pisang Husda Marwan; Meity S. Sinaga; . Giyanto; Abdjad Asih Nawangsih
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Blood disease is one of the important diseases of banana in Indonesia. Endophytic bacteria have potencies as candidates of biocontrol agents to blood disease, because the bacteria colonized the same ecological niche with the plant pathogens. This research was conducted to isolate endophytic bacteria from banana root, and study their disease suppression ability to blood disease on banana. Ninety isolates of endophytic bacteria have been isolated from the root of banana. Average population densities of bacteria varied between 6,0 x 103 and 4,2 x 105 cfu/g fresh weight of root. Twenty seven isolates positively produced inhibition zone toward blood disease bacterium. Based on plant growth and disease suppression test, ten isolates promoted the growth of banana plant and four isolates suppressed the incidence of blood disease with ranged from 66,67 to 83,33%.

Laporan Pertama Fitoplasma Berasosiasi dengan Penyakit Proliferasi Stroberidan Sapu Jalantir di Jawa Barat, Indonesia: First Report of Phytoplasmas Associated with Strawberry Proliferationand Tall Fleabane Witches’ broom Diseases in West Java, Indonesia Noor Sholihin; Kikin Hamzah Mutaqin; Giyanto
Jurnal Fitopatologi Indonesia Vol. 22 No. 1 (2026): Maret 2026: IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.22.1.1-6

Fitoplasma adalah kelompok bakteri dari kelas Mollicute yang telah banyak dilaporkan berasosiasi dengan penyakit pada ratusan spesies tanaman di berbagai negara. Di Indonesia, fitoplasma baru diketahui berasosiasi dengan tanaman-tanaman inang lebih dari delapan famili berbeda. Penelitian ini bertujuan membuktikan asosiasi fitoplasma pada tanaman stroberi bergejala filodi dan suatu tanaman liar bergejala daun kecil di Jawa Barat dengan teknik nested-polymerase chain reaction (nested-PCR) menggunakan dua pasangan primer yang dirancang dari wilayah gen 16S-23S rRNA fitoplasma. Tumbuhan liar juga diidentifikasi secara morfologi dan molekuler berdasarkan wilayah ITS gen ribosomal RNA. Gejala penyakit pada stroberi (Fragaria ananassa) meliputi proliferasi tunas, filodi dan buah kerdil, sedangkan pada tumbuhan liar berupa sapu. Tumbuhan liar yang dikenal masyarakat sebagai jalantir berhasil diidentifikasi sebagai Conyza sumatrensis berdasarkan morfologi dan analisis nukleotida amplikon hasil PCR menggunakan pasangan primer universal ITS1/ITS4. Amplifikasi PCR awal dengan pasangan primer P1/P7 untuk fitoplasma dari stroberi dan jalantir menghasilkan pita DNA positif berukuran ±1800 pb dengan ketebalannya yang inkonsisten. Amplifikasi lanjut dengan nested-PCR dengan pasangan primer R16F2n/R16R2 menghasilkan pita DNA berukuran ±1250 pb yang lebih tebal. Hasil nested-PCR mengonfirmasi bahwa fitoplasma berasosiasi dengan dua tanaman tersebut. Penelitian ini merupakan laporan pertama fitoplasma berasosiasi dengan tanaman stroberi (Rosaceae) dan jalantir (Asteraceae).

Efektivitas Ekstrak Tumbuhan untuk Mengeliminasi Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Aprizal Zainal; Aswaldi Anwar; Satriyas Ilyas; , Sudarsono; , Giyanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Objectives of experiments were to evaluate (1) in vitro inhibitory effects of plant extracts on Clavibacter michiganensis subsp. michiganensis (Cmm), (2) inhibitory effects of plant extracts on Cmm infected tomato seeds, and (3) effectiveness of seed treatment plus plant extracts to eliminate Cmm. After evaluating 20 plant extracts, curcuma rhizome and betel vine leaf extract, cinnamon and clove oil were selected for further test. Tomato seeds were artificially inoculated with Cmm to obtain high level of infection. Part of the seeds were dipped in either suspension of selected extracts for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 °C and RH 60-70%) plus either of plant extracts oils, respectively. Elimination of Cmm level from infected seeds was observed at 10 days after treatments. Results of the experiment indicated curcuma extract, betel vine extract, cinnamon oil or clove oil showed in vitro inhibitory effects on Cmm. Moreover, dipping infected seeds in either 5% of curcuma, betel vine extract, or 0.5% of clove oil or matriconditioning plus these extracts oils were effective to eliminate Cmm from infected seeds. These treatments may potentially be used and developed commercially for eliminating seedborne Cmm on infected tomato seeds. Keywords: Bacterial cancer, seedborne-pathogen, seed-treatments

Resistance of Capsicum annuum genotypes against various isolates of Phytophthora capsici from Java, Indonesia Wartono Tono; SURYO WIYONO; MUHAMAD SYUKUR; GIYANTO; PUJI LESTARI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 12 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201235

Abstract. Wartono, Wiyono S, Syukur M, Giyanto, Lestari P. 2019. Resistance of Capsicum annuum genotypes against various isolates of Phytophthora capsici from Java, Indonesia. Biodiversitas 20: 3723-3730. Phytophthora capsici Leonian is one of the pathogenic oomycetes that can reduce chilli production worldwide. In this study, Indonesian local chilli genotypes were evaluated for their resistance to P.capsici on chilli in Java, under controlled conditions. P. capsici used in this study consisted of four isolates derived from chilli plants in Java Island. The chilli genotypes tested were 38 local chilli (Capsicum annuum L.) originated from Indonesia and 3 introduced C. annuum. Inoculation was carried out by drenching 5 ml suspension of zoospore (1 x 104) on the growing medium suitable for the oomycetes around the plant. Disease symptom was measured at 21 days after inoculation. Salicylic acid and b-1.3-glucanase analyses were conducted to 7-week old plant with specific interval. Molecular characterization to complement disease assay was carried out using SCAR primers (OpD04,717-F/D04,717-R). Significant differences were found in genotypes, isolates, and interactions between chilli genotypes x P. capsici isolates. Four tested isolates had different levels of virulence ranging from moderate (CpnsCK1, KdrRM3, WnsbCk) to high (WnsbCK2). The chilli genotypes revealed high partial resistance to P. capsici, as demonstrated by six genotypes resistant to CpnsCK1, two genotypes to KdrRM3, and one genotype resistant to WnsbCK1. Resistance of one of chilli pepper, Ungara was positively associated with an increase of salicylic acid and b-1.3-glucanase. There is no association between the SCAR marker with resistance of chilli to P. capsici suggested these pathogens from Java are probably controlled by another locus which is not detected by this SCAR marker.

Co-Authors Abdjad Asih Nawangsih Aprizal Zainal Aswaldi Anwar Dwi Andreas Santosa Haliatur Rahma, Haliatur Husda Marwan KIKIN HAMZAH MUTAQIN Meity S Sinaga Meity S. Sinaga Memen Surahman Muhamad Syukur Noor Sholihin PUJI LESTARI RAHAYU WIDYASTUTI Satriyas Ilyas Sudarsono, Sudarsono Suryo Wiyono Wartono Tono Wilhelmus Terang Arga Sanjaya

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search