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All Journal AGRISAINS Jurnal Ilmiah AgriSains
Yusuf, Sunarti
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Agriculture, Biological Sciences & Forestry

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Evaluation of CpG DNA for Increasing Immune Gene Expression in Grouper (Epinephelus fuscoguttatus) Jamaluddin, Ruqayyah; Yusuf, Sunarti; Nursida, Nur Fajriani; Suryahman, Agus; Tahya, Akbar Marzuki
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17.935 KB)

Abstract

CpG DNA is a kind of DNA synthetic immunostimulant containing CpG motifs consisting of short sequences containing one or more CpG motifs which act as a danger signal to the natural and innate immune systems. This study aimed to analyze the ability of CpG DNA to increase Interleukin 1β (IL1β) gene expression in tiger grouper so that it could be used as immunostimulant. Analysis of the expression level was carried out by extracting RNA- from the cephalic kidney which was treated with CpG DNA or with PBS as a control. Complementary DNA (cDNA) synthesis was carried out using the Ready To Go You Prime First Strand Beads (GE Healthcare) kit using the RT-PCR technique. The PCR product was analized by measuring the photostimulated luminescence value using UN SCAN IT software based on the thickness of the DNA fragments. The gene expression level and photostimulated luminescence value of the IL1β gene were higher than that of the PBS treatment used as a control. The luminescence value of the photostimulated IL1β gene in CpG DNA treatment was 113684 while the PBS treatment as a control was only 95610.
Phylogenetic Analysis of Mud Crab Scylla olivacea with Several Crustacean Based mRNA Encoding FAMeT Yusuf, Sunarti; Tahya, Akbar Marzuki; Jamaluddin, Ruqayyah
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17.935 KB)

Abstract

Previous studies have succeeded in isolating, and the basic sequence of mRNA encoding FAMeT Scylla olivacea. Several studies have noted that the FAMeT encoding mRNA sequence has differences in crustacean species. The aim of this study was to determine the relationship between S. olivacea and other crustaceans based on the FAMeT encoding mRNA sequence. The similarity of the FAMeT encoding mRNA sequences showed that several crustaceans had similarities to the FAMeT mRNA sequences of S. olivacea. The phylogeny tree analysis showed the existence of 2 clusters of the relationship between the mRNA encoding FAMeT of S. olivacea on the same branch as 2 other crab species: S. serrata and S. paramamosain. It was also found in Portunus trituberculatus and P. pelagicus and river prawn Macrobrachium rosenbergii. These findings indicated that the S. olivacea crab was monophyletic with 2 other mangrove crabs, namely the crab and the river prawn M. rosenbergii. So that describes a very close kinship relationship genetically. Meanwhile, the second branch consists of crustaceans such as Homarus americanus, Cherax quadricarinatus, Fenneropenaeus merguiensis, Metapenaeus ensis and Litopenaeus vannamei.
Physiological Response and Growth of Nile Tilapia Oreochromis Niloticus Fed with Feed Containing Fermented Banana Peel Flour: Peubah Fisiologis dan Pertumbuhan Ikan Nila Oreochromis niloticus yang diberi Pakan mengandung Tepung Kulit Pisang Terfermentasi Tahya, Akbar Marzuki; Serdiati, Novalina; Safir, Muhammad; Ibrahim, Fadhila A; Yusuf, Sunarti; Jamaluddin, Ruqayyah
Jurnal Ilmiah AgriSains Vol. 26 No. 2 (2025): Jurnal Ilmiah AgriSains
Publisher : Fakultas Peternakan dan Perikanan, Universitas Tadulako, Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/jiagrisains.v26i2.2025.92-100

Abstract

Keberhasilan suatu kegiatan budidaya terutama pada skala intensif salah satunya ditentukan oleh pakan buatan. Karenanya, pakan buatan menghabiskan porsi terbesar biaya operasional dalam suatu kegiatan budidaya. Penelitian tentang penggunaan bahan pakan alternatif banyak dilakukan untuk menekan besarnya biaya pakan ikan. Salah satunya adalah pemanfaatan limbah kulit pisang. Penelitian ini bertujuan untuk melihat untuk melihat pertumbuhan dan respon fisiologis ikan nila yabf diberi pakan mengandung tepung kulit pisang yang difermentasi Saccharomycess cerevisiae sehingga mendapatkan informasi yang lebih dalam mengenai potensi tepung kulit pisang terfermentasi untuk dijadikan bahan baku pakan menggantikan bahan baku pakan import yang memiliki harga tinggi seperti tepung ikan dan kedelai. Kulit pisang yang digunakan adalah jenis pisang kepok matang yang dihaluskan dan difermentasi denga ragi roti S. cerevisiae sebanyak 2%. Dosis yang digunakan adalah 0%, 10%, 15% dan 20%. Parameter yang diujikan adalah pertumbuhan bobot mutlak, TKO, gula darah dan IHS. pertumbuhan bobot mutlak, TKO, gula darah dan IHS terbaik terlihat pada perlakuan 15% kemudian diususl oleh perlakuan 20%, 10% dan terakhir adalah kontrol 0%. Dari hasil yang diperoleh dapat disimpulkan bahwa tepung kulit pisang terfermentasi dapat dijadikan sebagai bahan baku pakan untuk mengurangi penggunaan bahan baku pakan import seperti tepung ikan dan tepung kedelai.
Co-Authors Akbar Marzuki Tahya Ibrahim, Fadhila A Muhammad Safir Novalina Serdiati Nursida, Nur Fajriani Ruqayyah Jamaluddin Suryahman, Agus