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All Journal AGRISAINS OCTOPUS : Jurnal Ilmu Perikanan International Journal of Applied Biology Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Jurnal Ilmiah AgriSains FISHIANA Journal of Marine and Fisheries
Suryahman, Agus
Akuakultur, Fakultas Perikanan, Universitas Cokroaminoto, Makassar, Indonesia.
Agriculture, Biological Sciences & Forestry Automotive Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Social Sciences Other

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Evaluation of CpG DNA for Increasing Immune Gene Expression in Grouper (Epinephelus fuscoguttatus) Jamaluddin, Ruqayyah; Yusuf, Sunarti; Nursida, Nur Fajriani; Suryahman, Agus; Tahya, Akbar Marzuki
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17.935 KB)

Abstract

CpG DNA is a kind of DNA synthetic immunostimulant containing CpG motifs consisting of short sequences containing one or more CpG motifs which act as a danger signal to the natural and innate immune systems. This study aimed to analyze the ability of CpG DNA to increase Interleukin 1β (IL1β) gene expression in tiger grouper so that it could be used as immunostimulant. Analysis of the expression level was carried out by extracting RNA- from the cephalic kidney which was treated with CpG DNA or with PBS as a control. Complementary DNA (cDNA) synthesis was carried out using the Ready To Go You Prime First Strand Beads (GE Healthcare) kit using the RT-PCR technique. The PCR product was analized by measuring the photostimulated luminescence value using UN SCAN IT software based on the thickness of the DNA fragments. The gene expression level and photostimulated luminescence value of the IL1β gene were higher than that of the PBS treatment used as a control. The luminescence value of the photostimulated IL1β gene in CpG DNA treatment was 113684 while the PBS treatment as a control was only 95610.
Efficacy of attenuated bacteria vaccine against streptococcal infection in larvae tilapia (Oreochromis niloticus) Agus Suryahman; Andi Masriah; Khairun Nisaa; Rahmi Rahmi; Akmal Akmal
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.63-66

Abstract

One of the common diseases found in tilapia is a streptococcal disease caused by the Streptococcus agalactiae bacteria. Treatment of this disease is generally done by using antibiotics.  The use of antibiotics in controlling disease in fish can create resistant bacterial strains with certain doses. The use of vaccines is one of the alternative control methods in fish defence against infections. The purpose of this study was to analyze the efficacy of vaccination on seeds through immersion using attenuated active bacteria to increase immunity at the beginning of the spread of streptococcosis disease. The vaccine used was obtained from attenuated Streptococcus agalactiae bacteria through repeated passage ten times. Vaccination was carried out through oral vaccine. Challenge tests were carried out on days 7, 14, and 21 days after vaccination on tilapia larvae by immersion. The result indicated that vaccination using attenuated bacteria could increase lysozyme activity at each observation time compared to control. The mortality of post-challenged larvae on days 7, 14 and 21 post-vaccination was lower than that of unvaccinated seed. The percentage of RPS values up to the 14th day of observation has increased and tends to decrease on the 21st day.
Efficacy of attenuated bacteria vaccine against streptococcal infection in larvae tilapia (Oreochromis niloticus) Agus Suryahman; Andi Masriah; Khairun Nisaa; Rahmi Rahmi; Akmal Akmal
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.63-66

Abstract

One of the common diseases found in tilapia is a streptococcal disease caused by the Streptococcus agalactiae bacteria. Treatment of this disease is generally done by using antibiotics.  The use of antibiotics in controlling disease in fish can create resistant bacterial strains with certain doses. The use of vaccines is one of the alternative control methods in fish defence against infections. The purpose of this study was to analyze the efficacy of vaccination on seeds through immersion using attenuated active bacteria to increase immunity at the beginning of the spread of streptococcosis disease. The vaccine used was obtained from attenuated Streptococcus agalactiae bacteria through repeated passage ten times. Vaccination was carried out through oral vaccine. Challenge tests were carried out on days 7, 14, and 21 days after vaccination on tilapia larvae by immersion. The result indicated that vaccination using attenuated bacteria could increase lysozyme activity at each observation time compared to control. The mortality of post-challenged larvae on days 7, 14 and 21 post-vaccination was lower than that of unvaccinated seed. The percentage of RPS values up to the 14th day of observation has increased and tends to decrease on the 21st day.
Evaluation of CpG DNA for Increasing Immune Gene Expression in Grouper (Epinephelus fuscoguttatus): Evaluasi Pemberian CpG DNA untuk Meningkatkan Ekspresi Gen Imun pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Ruqayyah Jamaluddin; Sunarti Sunarti; Nur Fajriani Nursida; Agus Suryahman; Akbar Marzuki Tahya
Jurnal Ilmiah AgriSains Vol. 21 No. 1 (2020): April
Publisher : Fakultas Peternakan dan Perikanan, Universitas Tadulako, Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.802 KB) | DOI: 10.22487/jiagrisains.v21i1.2020.8-16

Abstract

CpG DNA merupakan imunostimulan DNA sintetik yang mengandung CpG motif, terdiri dari sekuen pendek satu atau lebih motif CpG dan berperan sebagai danger signal terhadap sistem kekebalan imun natural maupun bawaan. Penelitian bertujuan menganalisa kemampuan CpG DNA untuk meningkatkan ekspresi gen Interleukin 1β (IL1β) pada ikan kerapu macan agar dapat dijadikan sebagai imunostimulan. Analisis tingkat ekspresi gen dilakukan dengan cara mengekstrak RNA organ kepala ginjal yang diberi perlakuan CpG DNA dan perlakuan PBS sebagai kontrol.Sintesis DNA komplementer (cDNA) dilakukan menggunakan kit Ready To Go You Prime FirstStrand Beads (GE Healthcare) dengan teknik RT-PCR. Produk PCR selanjutnya dianalisa dengan UN SCAN IT berdasarkan ketebalan fragmen DNA. Tingkat ekspresi gen serta nilai luminescence photostimulated dari gen IL1β lebih tinggi dibandingkan dengan perlakuan PBS sebagai kontrol. Nilai luminescence photostimulated gen IL1β pada perlakuan CpG DNA yaitu 113684 sementarapada perlakuan PBS sebagai kontrol hanya sebesar 95610.
Efficacy of attenuated bacteria vaccine against streptococcal infection in larvae tilapia (Oreochromis niloticus) Agus Suryahman; Andi Masriah; Khairun Nisaa; Rahmi; Akmal
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.63-66

Abstract

One of the common diseases found in tilapia is a streptococcal disease caused by the Streptococcus agalactiae bacteria. Treatment of this disease is generally done by using antibiotics.  The use of antibiotics in controlling disease in fish can create resistant bacterial strains with certain doses. The use of vaccines is one of the alternative control methods in fish defence against infections. The purpose of this study was to analyze the efficacy of vaccination on seeds through immersion using attenuated active bacteria to increase immunity at the beginning of the spread of streptococcosis disease. The vaccine used was obtained from attenuated Streptococcus agalactiae bacteria through repeated passage ten times. Vaccination was carried out through oral vaccine. Challenge tests were carried out on days 7, 14, and 21 days after vaccination on tilapia larvae by immersion. The result indicated that vaccination using attenuated bacteria could increase lysozyme activity at each observation time compared to control. The mortality of post-challenged larvae on days 7, 14 and 21 post-vaccination was lower than that of unvaccinated seed. The percentage of RPS values up to the 14th day of observation has increased and tends to decrease on the 21st day.
Efficacy of attenuated bacteria vaccine against streptococcal infection in larvae tilapia (Oreochromis niloticus) Agus Suryahman; Andi Masriah; Khairun Nisaa; Rahmi; Akmal
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.63-66

Abstract

One of the common diseases found in tilapia is a streptococcal disease caused by the Streptococcus agalactiae bacteria. Treatment of this disease is generally done by using antibiotics.  The use of antibiotics in controlling disease in fish can create resistant bacterial strains with certain doses. The use of vaccines is one of the alternative control methods in fish defence against infections. The purpose of this study was to analyze the efficacy of vaccination on seeds through immersion using attenuated active bacteria to increase immunity at the beginning of the spread of streptococcosis disease. The vaccine used was obtained from attenuated Streptococcus agalactiae bacteria through repeated passage ten times. Vaccination was carried out through oral vaccine. Challenge tests were carried out on days 7, 14, and 21 days after vaccination on tilapia larvae by immersion. The result indicated that vaccination using attenuated bacteria could increase lysozyme activity at each observation time compared to control. The mortality of post-challenged larvae on days 7, 14 and 21 post-vaccination was lower than that of unvaccinated seed. The percentage of RPS values up to the 14th day of observation has increased and tends to decrease on the 21st day.
Effect of Bioslurry Concentration in Feed on The Growth and Survival of Milkfish (Chanos chanos Forsskal) Zaenab, St; Masriah, Andi; Suryahman, Agus
International Journal of Applied Biology Vol. 6 No. 2 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bioslurry is cow excreta waste processed anaerobically as a nutritional by-product of biogas production, which contains proteins, carbohydrates, fats and minerals. This study aims to determine the concentration of solid bioslurry in feed and the concentration of liquid bioslurry in feed containing solid bioslurry, which is optimal for increasing the growth and survival of milkfish. The results showed that the best feed with a concentration of 50% solid bioslurry resulted in a growth of 100.05 grams ± 6.98 and a survival rate of 96.67% ± 1.15. A significant growth increase in feed with a high bioslurry content indicates that bioslurry contains nutrients capable of increasing growth energy in milkfish. The growth of milkfish continues to increase along with the increasing concentration of bioslurry liquid waste added to the feed as a source of probiotics. Adding 50 ml of liquid bioslurry into 100 grams of feed resulted in the best growth of 165.33 grams ± 2.08. Liquid bioslurry contains probiotic microbial bioactivators and functions to increase growth.
PENGARUH PEMBERIAN PAKAN KOMERSIL YANG DIFERMENTASI TERHADAP PERTUMBUHAN, EFISIENSI PAKAN DAN SINTASAN IKAN NILA (Oreochromis niloticus) Suryahman, Agus; Nisaa, Khairun; Syamsuddin, Syamsuddin; Jaya, Jaya
OCTOPUS: JURNAL ILMU PERIKANAN Vol. 12 No. 1 (2023): OCTOPUS
Publisher : Universitas Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26618/octopus.v12i1.11852

Abstract

Fermentasi terhadap pakan ikan dapat meningkatkan kecernaan pakan sehingga mempengaruhi pertumbuhan ikan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan yang difermentasi terhadap laju pertumbuhan, efisiensi pakan, dan tingkat kelangsungan hidup (sintasan) ikan nila (Oreochromis niloticus). Penelitian ini dilaksanakan pada bulan Januari sampai dengan bulan Maret 2021. Pemeliharaan ikan dan pengujian kualitas air dilakukan di Laboratorium Basah Perikanan, Fakultas Perikanan Universitas Cokroaminoto Makassar. Pemeliharaan ikan dan pengumpulan data menggunakan metode RAL dengan 3 perlakuan dan 3 ulangan.Perlakuan A, yaitu pemberian pakan komersil tanpa pengayaan bakteri probiotik dan molase, perlakuan B : 50 ml probiotik/kg pakan + molase 100 ml, didiamkan selama 30 menit, dan perlakuan C: 30 ml probiotik/kg pakan + molase 100 ml, didiamkan selama 30 menit. Pemberian pakan perlakuan sebanyak 5% dari biomassa ikan dengan frekwensi 2 kali sehari (06.00 dan 16.00 wita). Pemberian pakan dilakukan selama 60 hari masa pemeliharaan  Sampling pertumbuhan ikan dilakukan setiap 7 hari selama pemeliharaan dengan menimbang bobot ikan. Parameter uji yang diukur pada penelitian ini adalah pertambahan bobot (W), efisiensi pakan (EP), sintasan (SR), serta laju pertumbuhan spesifik (SGR). Berdasarkan hasil uji analisis varian (ANOVA), diketahui bahwa perlakuan pemberian probiotik yang dikombinasikan dengan molase dalam pakan ikan nila secara signifikan berpengaruh nyata (P<0.05) terhadap pertumbuhan mutlak, pertumbuhan relative, dan efesiensi pakan. Namun pemberian probiotik dan molase dalam pakan tidak berpengaruh singnifikan terhadap sintasan ikan nila (P>0.05)
Aspek Reproduksi Ikan Kurisi (Nemipterus japonicus) di Perairan Selat Sunda Achmad, Nurilmi; Suryahman, Agus
FISHIANA Journal of Marine and Fisheries Vol. 2 No. 2 (2023): November
Publisher : Fakultas Perikanan UCM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61169/fishiana.v2i2.71

Abstract

Penelitian ini bertujuan untuk menjelaskan aspek biologi reproduksi ikan kurisi (Nemipterus japonicus) yang meliputi nisbah kelamin, tingkat kematangan gonad, indeks kematangan gonad dan ukuran pertama kali matang gonad. Penelitian ini dilakukan pada bulan April sampai dengan September 2022 di Perairan Selat Sunda dengan lokasi pengumpulan data di Pangkalan Pendaratan Ikan (PPI) Labuan, Kabupaten Pandeglang, Provinsi Banten. Dari 950 ekor ikan kurisi terdiri terdiri atas ikan jantan 434 ekor dan ikan betina 516 ekor dengan perbandingan nisbah kelamin 1:1,19. Ikan kurisi jantan yang matang gonad terdapat pada bulan April, Mei dan Juni sedangkan betina bulan April, Mei, Juni, Juli dan Agustus. Ikan-ikan yang tertangkap selama penelitian didominasi oleh ikan-ikan yang belum matang gonad (I dan II) yang belum sempat bereproduksi yaitu pada bulan Juli. Pada ikan kurisi jantan dan betina nilai rata-rata IKG mengalami peningkatan pada pengambilan sampel bulan April, Juni, dan Agustus hingga TKG IV sedangkan pada pengambilan sampel bulan Mei dan Juli ikan jantan dan betina mengalami penurunan indeks kematangan gonad pada TKG III. Ukuran pertama kali ikan kurisi jantan dan betina matang gonad adalah 338-339 mm dan 350-353 mm.
Co-Authors Achmad, Nurilmi Akbar Marzuki Tahya Akmal Akmal Akmal Andi Masriah Asgar, Adam Jaya Jaya Khairun Nisaa Lukman Daris Massiseng, Andi Nur Apung Nisaa, Khairun Nur Fajriani Nursida Nursida, Nur Fajriani Rahmi Rahmi Rahmi Ruqayyah Jamaluddin Sunarti Sunarti Syamsuddin Syamsuddin Yusuf, Sunarti Zaenab, St