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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Aquacoastmarine Jurnal Sumberdaya Hayati Community Development Journal: Jurnal Pengabdian Masyarakat Bioscientist : Jurnal Ilmiah Biologi Room of Civil Society Development AQUACOASTMARINE: Journal of Aquatic & Fisheries Sciences Jurnal Kastara Room of Civil Social Development Jurnal Sains dan Teknologi Pangan
WULAN, RAHAYU
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Public Health Social Sciences Other

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Isolasi dan Identifikasi Molekuler Bakteri Salmonella Asal Tempe Dage Berdasarkan Gen 16S rRNA Inayah, Mazidah Noer; Budiarti, Suci Indah; Wulan, Rahayu
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18200

Abstract

This study aimed to isolate Salmonella from empe dage, particularly products sold in traditional markets in the Banyumas region, Central Java, and to identify the isolates through molecular analysis of the 16S rRNA gene. Isolation of Salmonella from dage samples was conducted using culture-based methods on several types of selective media specific for Salmonella. Identification was performed through biochemical characterization using the API 20E system and molecular analysis based on PCR amplification of the 16S rRNA gene. The resulting 16S rRNA sequences were subsequently analyzed using the BLAST tool available on the NCBI platform, and phylogenetic relationships were reconstructed with MEGA X. The study successfully obtained one bacterial isolate (TD.1), exhibiting transparent colonies with black centers on SSA medium. Biochemical tests including catalase activity, sugar fermentation, H₂S production, and citrate utilization showed positive results. Further identification using the API 20E system confirmed that isolate TD.1 belonged to the genus Salmonella. Molecular analysis of the 16S rRNA gene identified isolate TD.1 as Salmonella enterica. In conclusion, isolate TD.1, recovered from tempe dage was identified as Salmonella enterica. The presence of Salmonella enterica in dage highlights a potential health risk and underscores the need for improved food hygiene in traditional markets.
Co-Authors Aji, Candra Purnomo Alifah, Nasywa Afif Anggistya, Kintan Sekar Anja Meryandini Armando, Eric Asri, Yuliana Budiarti, Suci Indah Cika, Adella Inayah, Mazidah Noer Muh Azril Muhammad Iqbal Fanani Gunawan Mujtahidah, Tholibah Nugraheni, Martha Arum Pramono, Pradipta Bayuaji Putri, Soraya Kusuma Reyhan, Akmal Setiani, Novia Ardhita Shobrina Silmi Qori Tartila Sitepu, Sandy Haganta Titi Candra Sunarti Wahyudi, Brillian Rossi Widianto, Ikhwan Nursalman Zusron, Muhammad