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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Kelautan Tropis Indonesian Journal of Natural Pigments Journal of Tropical Pharmacy and Chemistry
Prastyo Abi Widyananto
Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro
Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Other

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Activity Antioxidant on Pigments of Bacterial Symbionts of Soft Coral From Jepara Sea Masduqi, Ahmad Fuad; Franyoto, Yuvianto Dwi; Kusmita, Lia; Muchlisin, Sakti; Widyananto, Prasetyo Abi; Sulistyani, Sulistyani; Wijayanti, Diah Permata
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.43

Abstract

Soft corals have been known to produce secondary metabolites, some of which may have anticancer, antifouling, antibacterial and antioxidants activity. It has been suggested that natural products from marine invertebrates have striking similarities to metabolites of their association microorganisms. Symbiont bacteria on soft coral can produce bioactive compounds that play an important role in chemical ecology and as a marine natural product. Marine bacteria associated with soft coral collected from Jepara were successfully isolated on medium ZoBell 2216E and screened to synthesize the pigment. This approach has allowed the use of this organism as an environmentally friendly alternative source of new natural pigment. This study found 25 bacterial isolates from 6 types of soft coral. Out of 25 bacterial isolates, only 3 bacterium, positively contains pigments. Four isolates, PCl 1, PS2 1, and PSa 2. Pigments analysis with UV spectrophotometric method showed the wavelength of pigments were in the range 300-600 nm. Genomic DNA was isolated from these colonies and nested PCR of the DNA was performed to amplify the 16S rDNA. Antioxidant activity was tested with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. From the results of molecular identification by 16S rDNA method, it was shown that bacterium PCl 1, PS2 1, and PSa 2 was closely related to Pseudomonas stutzeri, Ponticoccus gilvus, Bacillus marisflavi with 99%, 99and 98% homology value. Antioxidant activity is as follows: PCl 1>PS2 1>PSA 2.
Biodegradation of Polyethylene Microplastic using Culturable Coral-Associated Bacteria Isolated from Corals of Karimunjawa National Park Prastyo Abi Widyananto; Sakti Imam Muchlissin; Agus Sabdono; Bambang Yulianto; Fauziah Shahul Hamid; Ocky Karna Radjasa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 4 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.4.237-246

Abstract

Polyethylene is a plastic material that was globally produced and is well known as a non-degradable pollutant product. Plastic pollution, primarily microplastics, have been distributed to coral reef ecosystems, where these areas are ecosystems with high productivity. Karimunjawa National Park in Indonesia is one of the protected areas for coral reef ecosystem habitat in Central Java, threatened by microplastic contamination. Recent studies have shown that coral-associated bacteria have an adequate ability to degrade marine pollutant materials. No one has reported that the use of indigenous coral-associated bacteria has the potential for microplastic biodegradation, especially low-density polyethylene microplastic materials. Hence, the objective of this study was to find the potential of microplastic biodegradation agents derived from coral-associated bacteria in Karimunjawa National Park area. Various coral life-forms were isolated in July 2020 from conservation areas and areas with anthropogenic influences. Bacterial isolates were screened using tributyrin and polycaprolactone as substrates to reveal potential microplastic degradation enzymes. The total isolation results obtained 92 bacterial isolates, and then from the result of enzyme screening, there were 7 active bacteria and only 1 bacteria that potential to degrade polyethylene. LBC 1 showed that strain could degrade by 2.25±0.0684 % low-density polyethylene microplastic pellet by incubating bacterial growth until the stationary phase. Identification of LBC 1 strain was carried out by extracting DNA and bacterial 16S rRNA sequences. Bacterial gene identification refers to Bacillus paramycoides with a similarity level in the National Center Biotechnology Information database of 99.44%. These results prove that hard coral association bacteria can degrade low-density polyethylene microplastics.
Kelimpahan Mikroplastik Pada Sedimen Ekosistem Terumbu di Taman Nasional Laut Karimunjawa Sakti Imam Muchlissin; Prastyo Abi Widyananto; Agus Sabdono; Ocky Karna Radjasa
Jurnal Kelautan Tropis Vol 24, No 1 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i1.9865

Abstract

Eleven billion microplastic particles are entangled in coral reef ecosystems in the Asia - Pacific Region. The presence of microplastics in coral reef ecosystems in Indonesia, especially in the Karimunjawa Marine National Park, was found as many as 22.7 and 12.8 particles / kilogram samples in two locations. The presence of microplastics in coral reef ecosystems allows threats to the health of coral reefs. Therefore, the importance of this study is to complement the data on the distribution of microplastics in the Karimunjawa Marine National Park as an initial step for conservation and mitigation of the impact of plastic/ microplastic pollution. Sampling using purposive sampling method. Sediment collection using SCUBA set and sediment grab at a depth of 3 - 5 meters. ± 1000 g of sediment was taken and stored in double zip lock plastic. The samples were then analyzed by microplastics in the Tropical Marine Biotechnology Laboratory, FPIK UNDIP. The results showed that the greatest abundance of microplastics was in the Karang Tengah area with the number of microplastics 96 particles / kilogram, and the farthest in the Ujung Gelam and Lego was 11 particles / kilogram. Research shows that the abundance of microplastics in areas with human activities such as tourism, ports, and boat routes has a high abundance compared to conservation areas or areas with little human activity. It is hoped that the available data from this type of research will be able to produce decisions on conservation measures in Karimunjawa Marine National Park.  Sebelas miliar partikel mikroplastik terjerat pada ekosistem terumbu karang di Kawasan Asia – Pasifik. Keberadaan mikroplastik pada ekosistem terumbu karang di Indonesia, khususnya di Taman Nasional Laut Karimunjawa, ditemukan sebanyak 22,7 dan 12,8  partikel/kilogram sampel di dua lokasi. Keberadaan mikroplastik di ekosistem terumbu karang memungkinkan adanya ancaman terhadap kesehatan terumbu karang. Oleh karena itu pentingnya penelitian ini untuk melengkapi data sebaran mikroplastik di Kawasan Taman Nasional Laut Karimunjawa sebagai langkah awal konservasi dan mitigasi dari dampak polusi plastik/mikroplastik. Pengambilan sampel menggunakan metode purposive sampling. Pengambilan sedimen menggunakan SCUBA set dan sediment grab pada kedalaman 3 – 5 meter. Sedimen diambil ± 1000 gr dan disimpan dalam plastik double zip lock. Sampel kemudian dianalisis mikroplastik di laboratorium Tropical Marine Biotechnology, FPIK UNDIP. Hasil menunjukkan bahwa kelimpahan mikroplastik terbesar pada daerah karang tengah dengan jumlah mikroplastik 96 partikel/kilogram, dan terkecil pada perairan ujung gelam dan lego yaitu sebanyak 11 partikel/kilogram. Penelitian menunjukkan bahwa kelimpahan mikroplastik didaerah dengan aktivitas manusia seperti pariwisata, pelabuhan, dan jalur kapal memiliki kelimpahan tinggi dibanding dengan daerah konservasi atau daerah dengan aktivitas manusia yang kecil. diharapkan dengan data yang tersedia dari penelitian sejenis ini, mampu menghasilkan keputusan terhadap langkah konservasi di Taman Nasional Laut Karimunjawa. 
Seasonal Monitoring of Ocean Parameter Over Green Mussel Cultivation area in West Part of Cirebon Seawater Mochamad Riam Badriana; Ashadi Arifin Nur; Arsy Ilham Hidayatullah; Abi Widyananto Prastyo; Lamona Imudyati Bernawis; Chungkyun Jeon; Ivonne Milichristi Radjawane; Hansan Park
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 1 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.1.69-80

Abstract

West part of Cirebon in the past suffered an extraordinary event due to poisonous food found in green mussel. It is necessary to monitor current water quality over this region since this area still used for green mussel cultivation area. In-situ measurement and laboratory analysis were done to monitor the condition of seawater around Gunungjati as part of green mussel cultivation area in Cirebon regency. Several points had been measured through CTD Valeport Midas+ in prior to measure physical (temperature, salinity, pH, chlorophyll-a, turbidity) and chemical seawater (dissolved oxygen, Nitrate, Orthophosphate, Hg, Pb, Cd concentration) characteristics, meanwhile water sample had been brought to laboratory to be checked its heavy metal concentration. Additionally, past study and data related to green poison event in Cirebon was taken account into observation and analysis. Seasonal result show slightly different value though vertical distribution data where temperature (30.1°–32.3°C), salinity (26.9–32.0 psu), and pH (7.5–8.7) are high in December but lower in July, meanwhile, chlorophyll-a (0.2–20.9 mg.L-1), dissolved oxygen (0.2–7.8 mg.L-1), and turbidity (1.3–20.1 NTU) are increasing in October. Nitrate and orthophosphate concentration find in water sample is 0.097–0.537 mg.L-1 and <0.01–0.32 mg.L-1, respectively. Parameters of Cd (<0.001 mg.L-1), Hg (<0.00014 mg.L-1), and Pb (<0.001 mg.L-1) are still below the threshold. The condition of seawater of west part of Cirebon or at cultivation area is still in moderate condition based on the national standard. Although, turbidity and dissolved oxygen is still above the standard quality.
Bacterial Symbionts of Soft Coral Lobophytum Sp. From Panjang Island, Jepara, Indonesia With Antimicrobial MDR TB Potency Yuvianti Dwi Franyoto; Ahmad Fuad Masduqi; Sakti Muchlisin; Prasetyo Abi Widyananto; Sulistiyani Sulistiyani; Diah Permata Wijayanti; Lia Kusmita
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 5 (2019): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i5.200

Abstract

Tubercolusis is a disease that attacks the lungs. The disease is caused by the bacterium mycobacterium tubercolusis. The bacteria mycobacterium tubercolusis can be killed by antibiotics. However, continuous use of antibiotics can cause bacterial resistance. So we need to find new drugs that can prevent multi-drug resistant tuberculosis. This study aims to determine the antibacterial activity of MDR TB from soft coral symbiont bacteria Lobophytum sp. There were 6 bacterial isolates obtained from soft coral Lobophytum sp. One isolate from Lobophytum-associated bacteria were successfully screened for antimycobacterial against MDR TB bacteria. PLO2 was found to inhibit the growth of MDR TB (MDR TB strain SIRE and R). Based on the results of identification with PCR, soft coral symbionts of PLO2 was closely related to Virgibacillus marismortui with homology of 99%.
Co-Authors Agus Sabdono Ahmad Fuad Masduqi Arsy Ilham Hidayatullah Ashadi Arifin Nur Bambang Yulianto Chungkyun Jeon Diah Permata Wijayanti Diah Permata Wijayanti Fauziah Shahul Hamid Franyoto, Yuvianti Dwi Franyoto, Yuvianto Dwi Hansan Park Ivonne Milichristi Radjawane Lamona Imudyati Bernawis Lia Kusmita Lia Kusmita Mochamad Riam Badriana Muchlisin, Sakti Ocky Karna Radjasa Sakti Imam Muchlissin Sakti Imam Muchlissin Sakti Muchlisin Sulistiyani Sulistiyani Sulistyani Sulistyani