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UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN KANDUNGAN ANTOSIANIN TOTAL KULIT BUAH MANGGIS (Garcinia mangostana L) Kusmita, Lia; Wulansari, Endang Dwi; Supiyanti, Wiwin
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Buah manggis dengan nama latin Garcinia mangostana L. mendapat julukan Queen of tropical fruit (Ratunya Buah-buahan Tropik). Kulit buah manggis berwarna ungu kehitaman. Diduga kulit buah manggis mengandung senyawa antosianin. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak kulit buah manggis dan kandungan antosianin total dalam kulit buah manggis. Obyek penelitian adalah kulit buah manggis yang sudah layak untuk dikonsumsi. Kulit buah manggis dihaluskan kemudian direndam dengan pelarut metanol yang mengandung HCl 1%. Dilakukan uji aktivitas antioksidan menggunakan metode DPPH dan penentuan kandungan antosianin total dalam kulit buah manggis menggunakan metode perbedaan pH. Uji aktivitas antioksidan ekstrak kulit buah manggis meliputi uji aktivitas antioksidan secara kualitatif berupa lempeng KLT ekstrak kulit buah manggis disemprotkan penampak bercak DPPH 0,1 mM menghasilkan bercak kuning pucat dengan latar belakang ungu. Uji aktivitas antioksidan secara kuantitatif berdasarkan parameter EC50 menggunakan metode DPPH dengan pembanding vitamin C. Diperoleh nilai EC50 sebesar 8,5539 Î¼g/mL dan nilai EC50 vitamin C adalah 3,3676 Î¼g/mL. Rata-rata kandungan antosianin total dalam kulit buah manggis adalah 59,3 mg dalam 100 gram kulit buah manggis.Â

Uji Aktivitas Antibakteri Pigmen Karotenoid Dari Bakteri Simbion Karang Lunak Sarcophyton sp. Terhadap Pertumbuhan Bakteri Patogen Staphylococcus aureus ATCC 25923 Wiguna, Awang Surya; Kusmita, Lia; Radjasa, Ocky Karna
Indonesian Journal of Pharmaceutical Science and Technology Vol 3, No 3 (2016)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Mikroba penghasil antibiotik dapat berupa fungi maupun bakteri yang bersimbiosis pada organisme lain. Pigmen karotenoid dari bakteri simbion karang lunak merupakan mikroorganisme yang berpotensi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri ekstrak pigmen karotenoid dari bakteri simbion karang lunak Sarcophyton sp. terhadap pertumbuhan bakteri patogen Staphylococcus aureus ATCC 25923 serta mengetahui perbedaan aktivitas antibakteri ekstrak pigmen karotenoid pada konsentrasi 0,5 %, 0,75 %, dan 1 % dengan metode sumuran. Bakteri yang menghasilkan pigmen karotenoid dibiakkan dan diidentifikasi dengan mengisolasi DNA untuk dilakukan PCR dan mensequen hasilnya. Hasil penelitian aktivitas antibakteri dengan rata â rata diameter zona hambat pada konsentrasi 0,5 % sebesar 0,678 cm, konsentrasi 0,75 % sebesar 0,978 cm, konsentrasi 1 % sebesar 1,416 cm serta diameter zona hambat kontrol positif amoksisilin trihidrat sebesar 1,875 cm.

Activity Antioxidant on Pigments of Bacterial Symbionts of Soft Coral From Jepara Sea Masduqi, Ahmad Fuad; Franyoto, Yuvianto Dwi; Kusmita, Lia; Muchlisin, Sakti; Widyananto, Prasetyo Abi; Sulistyani, Sulistyani; Wijayanti, Diah Permata
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.43

Soft corals have been known to produce secondary metabolites, some of which may have anticancer, antifouling, antibacterial and antioxidants activity. It has been suggested that natural products from marine invertebrates have striking similarities to metabolites of their association microorganisms. Symbiont bacteria on soft coral can produce bioactive compounds that play an important role in chemical ecology and as a marine natural product. Marine bacteria associated with soft coral collected from Jepara were successfully isolated on medium ZoBell 2216E and screened to synthesize the pigment. This approach has allowed the use of this organism as an environmentally friendly alternative source of new natural pigment. This study found 25 bacterial isolates from 6 types of soft coral. Out of 25 bacterial isolates, only 3 bacterium, positively contains pigments. Four isolates, PCl 1, PS2 1, and PSa 2. Pigments analysis with UV spectrophotometric method showed the wavelength of pigments were in the range 300-600 nm. Genomic DNA was isolated from these colonies and nested PCR of the DNA was performed to amplify the 16S rDNA. Antioxidant activity was tested with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. From the results of molecular identification by 16S rDNA method, it was shown that bacterium PCl 1, PS2 1, and PSa 2 was closely related to Pseudomonas stutzeri, Ponticoccus gilvus, Bacillus marisflavi with 99%, 99and 98% homology value. Antioxidant activity is as follows: PCl 1>PS2 1>PSA 2.

FUCOIDAN NANOENCAPSULATION FROM BROWN ALGAE (Sargassum polycystum) AS A POTENTIAL MARINE IMMUNOMODULATORY AGENT Purwanto, Ungsari Rizki Eka; Ikasari, Endang Diyah; Bagiana, I Kadek; Kusmita, Lia; Trisnayanthi, Ni Nyoman Ayu Indah; Mudianta, I Wayan; Prasetijo, Rahmadi
Jurnal Ilmiah Farmako Bahari Vol 15, No 1 (2024): Jurnal Ilmiah Farmako Bahari
Publisher : Faculty of Mathematic and Natural Science, Garut University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jifb.v15i1.3207

The quest for better and more effective treatments has encouraged the search for therapies derived from natural sources to obtain effective immune therapy, considering that several pandemics have arisen caused by viruses. Developing fucoidan from brown algae in drug encapsulation as an immunomodulator could be more promising. This study aimed to produce nanoencapsulation loaded with fucoidan-purified extract from brown algae and to evaluate the influence of nanoencapsulation formulation on the immunomodulatory activity of fucoidan. Fucoidan was obtained from brown algae and extracted by hot aqueous, followed by ethanol purification. Nanoencapsulation of fucoidan purified extract was prepared using the ionic gelation method. The carbon clearance method was carried out for the immunomodulatory activity test of the nanoencapsulation of fucoidan purified extract. Nanoencapsulation of fucoidan purified extract with the optimum composition of maltodextrin 9.9% and S-TPP 0.1% (1:5) resulted in particle size of 715.4 nm, zeta potential -0.1 mV, pH 7.54, transmittance 97.54%+0.08, and entrapment efficiency 89.94%+0.17. The carbon clearance test showed that the nanoencapsulation of fucoidan was a strong immunostimulant with a phagocytosis index of 1.65. The development of nanoencapsulation could increase the phagocytosis index of fucoidan purified extracts from brown algae. Further molecular studies are needed to demonstrate the molecular activity of this preparation as an immunomodulator.

Pelatihan Pembuatan Jamu Saritoga dan Penyuluhan Sistem Imun Pada Ibu PKK: Kusmita, Lia; Dwi Franyoto, Yuvianti; Mutmainah, Mutmainah; Puspitaningrum, Ika; Bagiana, I kadek
JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Vol 3 No 2 (2024): April
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Latar belakang: Jamu adalah sebutan untuk obat tradisional dari Indonesia. Jamu dibuat dari bahan alami dari tumbuhan seperti rimpang (akar-akaran), daun-daunan, kulit batang dan buah. Diperkirakan sekitar 70-80% populasi di negara berkembang memiliki ketergantungan pada obat tradisional. Secara umum jamu dianggap tidak beracun dan tidak menimbulkan efek samping. Khasiat jamu telah teruji oleh waktu, zaman dan sejarah, serta bukti empiris langsung pada manusia selama ratusan tahun. Tanaman obat keluarga atau yang lebih populer sebagai TOGA merupakan tanaman obat yang ditanam di lingkup lingkungan keluarga. Pada masa pandemi, terjadi tren peningkatan penggunaan obat tradisional untuk menjaga daya tahan tubuh. Berbagai pembuktian secara ilmiah tentang manfaat tanaman obat dalam hal menjaga daya tahan tubuh. Tujuan: Meningkatkan wawasan dan pengetahuan masyarakat mengenai TOGA serta manfaatnya, termasuk cara pengolahan dan pembuatan jamu Saritoga.. Metode: Pelaksanaan kegiatan dilakukan secara langsung melalui sosialisasi dan pelatihan langsung kepada kelompok masyarakat sasaran. Hasil: Masyarakat yang awalnya tidak memahami sistem imun jadi lebih paham. Hasil pretest nilai jawaban yang benar adalah 45% setelah dilakukan pelatihan dan penyuluhan nilai jawaban yang benar meningkat menjadi 85%. Kesimpulan: Pelatihan dan penyuluhan dapat meningkatkan pengetahuan perihal system imun dan ketrampilan pembuatan jamu Saritoga pada masyarakat. Kata kunci: pelatihan, penyuluhan, saritoga, sistem imun _____________________________________________________________________________________ Abstract Background: Jamu is the name for traditional medicine from Indonesia. Herbal medicine is made from natural plant ingredients such as rhizomes (roots), leaves, bark, and fruit. It is estimated that around 70-80% of the population in developing countries is dependent on traditional medicine. Herbal medicine is considered non-toxic and does not cause side effects. The efficacy of herbal medicine has been tested by time, time, and history, as well as direct empirical evidence on humans for hundreds of years. Family medicinal plants or TOGA are medicinal plants grown within the family environment. During the pandemic, there has been a trend of increasing the use of traditional medicine to maintain the body's immune system. The benefits of medicinal plants in keeping the body's immune system are scientific evidence. Objective: Increase public insight and knowledge regarding TOGA and its benefits, including how to process and make Saritoga herbal medicine. Method: Activities implementation is directly through direct outreach and training to target community groups. Result: People who initially did not understand the immune system became more understanding. The pretest result of the correct answer value was 45%. After training and counseling, the correct answer value increased to 85%. Conclusion: Training and counseling can increase knowledge about the immune system and skills in making Saratoga herbal medicine in the community. Keywords: training, counseling saritoga, immune system

EFEK ANALGETIK TEPUNG UMBI BIDARA UPAS (Merremia mammosa (Lour) Hall. F.) PADA MENCIT JANTAN Puspitaningrum, Ika; Kusmita, Lia
Media Farmasi Indonesia Vol. 9 No. 2 (2014): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.745 KB)

Abstract Analgesic is a compound that can reduce or eliminate pain without losing consciousness. Bidara upas (Merremia mammosa (Lour) Hall. F.) is one of the flora that part edible tubers and useful for the treatment of, among others, anti-inflammatory and analgesic. The content of flavonoids and alkaloids in the tubers bidara upas could be expected to have an effect as an analgesic. This study aims to determine the analgesic effect bidara upas tuber starch (BUTS) and starch tubers effective dose bidara upas as an analgesic. This study was an experimental study with random sampling technique (random sampling). Testing of analgesic effect (BUTS) using 25 male mice Swiss strain which is divided into 5 groups. Group I, II, and III as a dose of 1, 2 and 3 are a group (BUTS) giving a dose of 1,5; 3; and 6 g/kgBB, IV as a negative control group is a group of distilled water provision, as well as the positive control group V is a group of administration of paracetamol dose 91 mg/kgBB. All treatments are given orally. 5-minute intervals after treatment, all mice were given painful stimuli in the form of a sterile solution of glacial acetic acid 1% v / v with a dose of 300 mg/kgBB and observed stretching that arise every interval of 5 minutes for 60 minutes. Total cumulative stretching all treatment groups were analyzed statistically with SPSS 16 with a 95% confidence level. It also calculated percent analgesic power all treatment groups. The results obtained are significant differences between the groups with a negative control group tuber flour upas bidara three doses. This proves tuber flour bidara upas have analgesic effect. In addition, the results obtained are significant differences between the positive control group by group tuber flour upas bidara three doses. Tuber bidara upas starch total cumulative dose has smaller stretching and % power analgesic greater than the paracetamol group. Based on these results, the effective dose tuber flour bidara upas as analgesic of 1,5 g/kgBB of mice.

OPTIMASI BASIS KRIM EKSTRAK ETANOL DAUN KAWISTA (Limonia acidissima L.) SEBAGAI ANTIBAKTERI Staphylococcus aureus Nugraheni, Christi Karina; Ikasari, Endang Diyah; Kusmita, Lia
Media Farmasi Indonesia Vol. 11 No. 1 (2016): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.561 KB)

SARIDaun kawista diketahui mengandung senyawa flavonoid, tannin, danalkaloid yang dapat menghambat pertumbuhan Staphylococcus aureus.Penggunaan emulgid dan parafin cair dalam sediaan krim sebagai emulgator danpelembut diharapkan mampu meningkatkan aktivitas antibakteri Staphylococcusaureus dan karakteristik fisik krim yang memenuhi syarat.Penelitian ini bertujuanuntuk mengetahui pengaruh dan menentukan formula optimal dengan komponenemulgid dan parafin cair dalam krim ekstrak etanol daun kawista (Limoniaacidissima L.) pada karakteristik fisik dan aktivitas antibakteri Staphylococcusaureus dengan metode Simplex Lattice Design. Ekstrak etanol daun kawistadidapatkan dengan cara remaserasi menggunakan pelarut etanol 70%. Ekstrakkental diuji aktivitas antibakteri Staphylococcus aureus dengan metode difusisumuran kemudian konsentrasi ekstrak yang optimal diformulasikan dalamsediaan krim. Berdasarkan Design Expert 9.0.6.2 Trial, didapatkan formulaoptimal dengan perbandingan konsentrasi emulgid dan parafin cair sebesar24,848%:5,152%. Hasil pengujian formula optimal didapatkan rerata diameterzona hambat bakteri 11,50 mm, daya sebar 6,49 cm, viskositas 4480 cps, dan pH5,46 dimana hasil tersebut berbeda tidak signifikan terhadap hasil prediksi DesignExpert 9.0.6.2 Trial.

Aktivitas Antibakteri Staphylococcus Aureus Dan Penetapan Kadar Antosianin Total Dari Bunga Kembang Sepatu (Hibiscus Rosa-Sinensis L.) : Aktivitas Antibakteri Antosianin dari Bunga Sepatu Andriyani, Silvi; Kusmita, Lia; Franyoto, Yuvianti
Media Farmasi Indonesia Vol. 18 No. 1 (2023): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53359/mfi.v18i1.214

Penyakit infeksi merupakan salah satu masalah kesehatan terpenting di negara berkembang seperti Indonesia. Salah satu penyebab penyakit infeksi adalah bakteri Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri Staphylococcus aureus dari ekstrak dan antosianin bunga kembang sepatu (Hibiscus rosa-sinensi L.). Metode ekstraksi pada penelitian ini adalah metode maserasi dengan pelarut metanol yang mengandung HCl 1% pekat. Pemisahan antosianin menggunakan metode Kromatografi Lapis Tipis Preparatif. Identifikasi antosianin dengan spektrofotometer UV-Vis. Uji aktivitas antibakteri ekstrak dan antosianin pada konsentrasi 9%, 12% dan 15%. Uji aktivitas antibakteri dilakukan dengan metode difusi sumuran. Pengujian antibakteri ekstrak bunga kembang sepatu pada konsentrasi 9%, 12%, dan 15% memiliki rata-rata zona hambat sebesar 0,637, 0,817, dan 1,006. Sedangkan antosianin konsentrasi 9%, 12%, dan 15% memiliki rata-rata zona hambat sebesar 0,818, 1,009, 1,194. Hasil uji ANOVA dua jalan diketahui bahwa terdapat beberapa yang berbeda signifikan dan berbeda tidak signifikan dalam memberikan aktivitas antibakteri (p<0,05) pada konsentrasi 9%, 12%, dan 15%. Hasil penetapan kadar antosianin total dari perasan bunga kembang sepatu diperoleh rata-rata yaitu sebesar 0,287 mg/mL. Ekstrak dan antosianin bunga kembang sepatu (Hibiscus rosa-sinensis L.) memiliki aktivitas antibakteri Staphylococcus aureus.

In Silico Study of Compounds Identified in Curcuma aeruginosa Roxb Rhizome as BRAF V600E Inhibitors in Melanoma Cancer Suharsanti, Ririn; Ryan Radix Rahardhian, Muhammad; Kusmita, Lia
Journal of Food and Pharmaceutical Sciences Vol 13, No 2 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.21589

Curcuma aeruginosa Roxb rhizome contains secondary metabolite compounds and plays a role in various activities such as antioxidant, antibacterial, anthelmintic, antiandrogenic, antinociceptive, and anticancer. Anticancer activity that has been reported in Curcuma aeruginosa Roxb rhizome is limited to breast and cervical cancer. The purpose of this study was to explore the potential of Curcuma aeruginosa Roxb rhizome in melanoma cancer through the mechanism of inhibiting the BRAF V600E. The 96% ethanol extract of Curcuma aeruginosa Roxb rhizome was separated to produce n-hexane (HF), ethyl acetate (EAF), and ethanol (EF) fractions. The GC-MS results showed that there were 31 compounds from the three fractions. The docking validation process was carried out on the native ligand N-(3-{[5-(4-chlorophenyl) -1H-pyrrolo [2,3b]pyridin3yl] carbonyl}2,4difluorophenyl) propane-1-sulfonamide. All compounds were prepared as ligands for molecular docking with the BRAF V600E receptor (PDB ID: 3OG7). Docking validation on native ligand showed RMSD 1.03Å. The smallest binding affinity are 4,4a,5,6,7,8-Hexahydronaphthalen-2(3H)-one (-6,89 kcal/mol); 1Cyclohexyl-2-propen-1-one (-6,68 kcal/mol); Cyclooctenone (-6,23 kcal/mol); and vemuravnib is still better as K+ (-11.11 kcal/mol). All three compounds do not bind to key amino acid residues of BRAF V600E such as vemuravenib at GLN A:530, CYS A:532; ASP A:594. These results indicate that further structural development is needed for better activity.

In Silico Study of Compounds Identified in Curcuma aeruginosa Roxb Rhizome as BRAF V600E Inhibitors in Melanoma Cancer Suharsanti, Ririn; Ryan Radix Rahardhian, Muhammad; Kusmita, Lia
Journal of Food and Pharmaceutical Sciences Vol 13, No 2 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.21589

Curcuma aeruginosa Roxb rhizome contains secondary metabolite compounds and plays a role in various activities such as antioxidant, antibacterial, anthelmintic, antiandrogenic, antinociceptive, and anticancer. Anticancer activity that has been reported in Curcuma aeruginosa Roxb rhizome is limited to breast and cervical cancer. The purpose of this study was to explore the potential of Curcuma aeruginosa Roxb rhizome in melanoma cancer through the mechanism of inhibiting the BRAF V600E. The 96% ethanol extract of Curcuma aeruginosa Roxb rhizome was separated to produce n-hexane (HF), ethyl acetate (EAF), and ethanol (EF) fractions. The GC-MS results showed that there were 31 compounds from the three fractions. The docking validation process was carried out on the native ligand N-(3-{[5-(4-chlorophenyl) -1H-pyrrolo [2,3b]pyridin3yl] carbonyl}2,4difluorophenyl) propane-1-sulfonamide. All compounds were prepared as ligands for molecular docking with the BRAF V600E receptor (PDB ID: 3OG7). Docking validation on native ligand showed RMSD 1.03Å. The smallest binding affinity are 4,4a,5,6,7,8-Hexahydronaphthalen-2(3H)-one (-6,89 kcal/mol); 1Cyclohexyl-2-propen-1-one (-6,68 kcal/mol); Cyclooctenone (-6,23 kcal/mol); and vemuravnib is still better as K+ (-11.11 kcal/mol). All three compounds do not bind to key amino acid residues of BRAF V600E such as vemuravenib at GLN A:530, CYS A:532; ASP A:594. These results indicate that further structural development is needed for better activity.

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