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All Journal Jurnal Ilmu Pertanian Indonesia Proceeding of International Conference on Biology Education, Natural Science, and Technology Journal of Biomedical Sciences and Health
Mariana, Afifah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities Taruna Dwi Satwika; Yulianti, Dwiana Muflihah; Hidayat, Galang Anahatta; Mariana, Afifah
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.163

Abstract

Lignin is a complex chemical heterogeneous polymer that forms a physical barrier to lignocellulose's biological and chemical hydrolysis, making lignocellulosic biomass challenging to degrade. Ligninolytic microorganisms play an essential role in lignin degradation by producing extracellular enzymes. Lignin peroxidase and manganese peroxidase are enzymes that play a role in lignin degradation. Forty-one bacterial isolates have been isolated from soil, kitchen waste, leaf litter, and cow dung. However, the ligninolytic activity of these isolates has yet to be discovered. This research aimed to determine the ligninolytic ability of bacteria isolated from soil, leaf litter, kitchen waste, and cow dung based on lignin peroxidase and manganese peroxidase activity. The study was conducted stages: isolate recultured, qualitative and quantitative testing of lignin peroxidase activity based on degradation of methylene blue dye, and qualitative and quantitative testing of manganese peroxidase activity based on degradation of phenol red dye. A total of four bacterial isolates from soil (Tn9, Tn14, Tn16, and Tn17) and two bacterial isolates from cow dung (KS2 and KS5) showed qualitative and quantitative lignin peroxidase activity. Manganese peroxidase activity was also shown by four isolates from soil (Tn2, Tn6, Tn14, and Tn16), one isolate from kitchen waste (SD1), and one isolate from cow dung (KS5) both qualitatively and quantitatively. The nine bacterial isolates that showed lignin peroxidase and manganese peroxidase activity have potential as lignin-degrading biological agents. Keywords: bacteria, ligninolytic, peroxidase
Expanding the Therapeutic Landscape: Exploring the Antimicrobial and Bioactive Potential of Mangrove-Derived Endophytic Fungi Rovik, Anwar; Mariana, Afifah; Hidayat, Galang Anahatta; Rahman, Farras Alifia
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2025: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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The escalating rise of antibiotic resistance poses a significant challenge to discovering new, effective antibiotics. This crisis represents one of the most critical threats to global health, potentially leading to a future where even minor infections could become fatal. Endophytic fungi have recently emerged as a promising source of novel bioactive compounds. This review highlights the potential of endophytic fungi isolated from mangrove vegetation to produce new antimicrobial agents. Mangrove-derived endophytic fungi are found in healthy leaves, hypocotyls, roots, stems, and flowers. The symbiotic relationship between mangrove vegetation and these fungi promotes the synthesis of diverse bioactive compounds, including newly discovered molecules such as cytospyrone, cytospomarin, penicibrocazines, thiocladospolides, coumarin, isocoumarins, and dihydroradicinin. Beyond their antimicrobial potential, these fungi also produce compounds with antifungal, antioxidant, anticancer, anti-inflammatory, anti-filarial, antibiofilm, influenza antiviral, antimycobacterial, and biological control properties. The traditional approach to antibiotic development is complex, challenging, costly, time-consuming, and labor-intensive. To overcome these obstacles, research must integrate machine learning for big data analysis and molecular-based exploration, including genomics, proteomics, and transcriptomics.
Enhanced Survival of Lactobacillus Lg71 from Mangrove Sediment Under Simulated Gastric and Intestinal Conditions Kusharyati, Dyah Fitri; Satwika, Taruna Dwi; Alfisah, Rizal Khoirun; Mariana, Afifah; Rovik, Anwar; Oedjijono, Oedjijono
Journal of Biomedical Sciences and Health Vol. 3 No. 1 (2026)
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/jbsh.v3.i1.291

Abstract

Background: For a probiotic to offer health benefits, it must stay viable through the tough environment of the human gastrointestinal tract. This study assesses the effectiveness of sodium alginate encapsulation in improving the survival of Lactobacillus LG71, a new strain from mangrove sediment, under simulated gastric and intestinal conditions. Objective: This study aims to evaluate the effectiveness of sodium alginate encapsulation in enhancing the viability and survival of Lactobacillus LG71, a probiotic strain isolated from mangrove sediment, during cold storage and under simulated gastric and intestinal conditions. Methods: Lactobacillus LG71 was encapsulated in sodium alginate beads. The viability of both encapsulated and free (non-encapsulated) cells was assessed over a 4-week storage period at 4 °C and during sequential exposure to simulated gastric and intestinal environments. Results: Encapsulation significantly improved survival rates compared to free cells (p < 0.05). Although both groups experienced a decline during the first week of storage, encapsulated cells maintained a high viability of approximately 107 CFU/mL, losing only 2.51 log CFU/g over four weeks. Most notably, encapsulated Lactobacillus LG71 demonstrated greater resilience during digestion, retaining populations of 2.21 log CFU/mL in gastric simulations and 1.00 log CFU/mL in intestinal simulations. In contrast, free cells were much more vulnerable to these acidic and enzymatic conditions. Conclusion: Sodium alginate encapsulation effectively protects Lactobacillus LG71 from environmental and biological stressors. These findings indicate that encapsulation is a crucial step for the commercial application of mangrove-derived probiotics, ensuring that a viable dose of living cells reaches the host's lower gastrointestinal tract to promote health benefits.
Co-Authors Alfisah, Rizal Khoirun Dyah Fitri Kusharyati Hidayat, Galang Anahatta Oedjijono Oedjijono, Oedjijono Rahman, Farras Alifia Rovik, Anwar Satwika, Taruna Dwi Taruna Dwi Satwika Yulianti, Dwiana Muflihah