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All Journal BioEksakta : Jurnal Ilmiah Biologi Unsoed Sciscitatio : Journal for Biological Science
Yulianti, Dwiana Muflihah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Identifikasi dan Uji Potensi Amilolitik Isolat Jamur Pendegradasi Sampah Organik Haqq, Insaaniy Mahdiyatul; Dewi, Ratna Stia; Mumpuni, Aris; Hikam, Arif Rahman; Yulianti, Dwiana Muflihah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4748

Abstract

Organic waste is composed of organic compounds. The accumulation of organic waste is a serious problem. Fungal have an important role in degrading organic waste in the composting process. Biodegradation of organic waste is closely related to fungal ability to hydrolyze starch. The purpose of this study was to know fungi isolate which hydrolyze starch from organic waste and amylolytic potential of the isolate. This research was conducted by survey and experimental method. Kitchen waste samples consist of food waste and other organic waste taken from homes in Bancarkembar, Bobosan, Grendeng, Karangwangkal, Pabuaran, Purwanegara, and Sumampir village. A screening test with Starch Agar medium was used to know amylolytic potential of the isolates. Result showed there were eight isolates which have potential to hydrolyze starch. Six isolates which have higher amylolitic index were identified as Fusarium sp., Aspergillus sp., and Penicillium sp. Furthermore, to determine the amylolytic activity quantitatively, the DNS method was used to measure glucose levels. Fusarium sp. had the highest starch degradation activity with the average glucose content of the medium Fusarium sp. as much as 3,568.63 ppm.
Isolasi, Skrining dan Identifikasi Fungi Selulolitik Asal Tempat Pengolahan Sampah Terpadu (TPST) Rempoah, Kabupaten Banyumas Hikam , Arif Rahman; Setio, Adinda Eka Murti; Mumpuni, Aris; Yulianti, Dwiana Muflihah; Dewi, Ratna Stia
SCISCITATIO Vol. 5 No. 2 (2024): Volume 5 Number 2, January 2024 (Online First)
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.52.175

Abstract

Tempat Pengolahan Sampah Terpadu (TPST) Rempoah, Kabupaten Banyumas memiliki timbunan sampah organik melimpah yang dikomposkan secara alami. Sampah organik dapat terdegradasi secara alami oleh beberapa mikroba, salah satunya adalah fungi selulolitik yang dapat mendegradasi selulosa dengan mekanisme enzimatis. Salah satu upaya untuk mengatasi permasalahan sampah organik yang melimpah adalah mengeksplorasi mikroba yang memiliki potensi dalam menguraikan sampah organik dengan efektif dan cepat. Tujuan dari penelitian ini adalah untuk memperoleh isolat fungi yang memiliki potensi selulolitik asal TPST Rempoah. Penelitian ini terdiri dari tahap pengambilan sampel; isolasi dan pemurnian fungi; skrining fungi selulolitik dengan menggunakan media CMC; dan identifikasi isolat fungi secara morfologi. Hasil penelitian didapatkan 6 isolat fungi selulolitik yang berasal dari Genus Aspergillus yang berhasil diisolasi dari TPST Rempoah. Nilai indeks selulolitik tertinggi dimiliki isolat Aspergillus RB1 dengan nilai IS sebesar 1,33.
Co-Authors Arif Rahman Hikam Aris Mumpuni Haqq, Insaaniy Mahdiyatul Hikam , Arif Rahman Ratna Stia Dewi Setio, Adinda Eka Murti