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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BIOPROSPEK Jurnal Biologi Tropis Jurnal HPT (Hama Penyakit Tumbuhan) Biocaster : Jurnal Kajian Biologi Jurnal Ilmiah Pangan Halal
Pujiyanti, Aloysia Sri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Veterinary Other

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Character Improvement of Red Rice (Oryza Sativa L.) Cv. Barak Cenana by Mutagenesis using Gamma Irradiation Aloysia Sri Pujiyanti; Bintang Kerta Wijaya; Ida Bagus Made Artadana; Popy Hartatie Hardjo; Maria Goretti Marianti Purwanto
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2554

Abstract

Red rice cv. Barak Cenana is a local Indonesian rice which is widely planted in Tabanan Regency, Bali. Barak Cenana red rice has potential as a functional food because it contains a lot of nutrients. However, this rice has an unfavorable character, such as long harvest time so that it can only be harvested once a year, and tall stature that causes plants to lodge easily, reducing its productivity. This study aims to determine the effect of gamma rays on growth and development as well as changes in character to obtain mutants plants that have better character. In this research, Barak Cenana seeds were irradiated using gamma rays with irradiation doses of 100 gy, 200 gy, 400 gy, and 800 gy. Furthermore, radiated seeds were planted in the greenhouse and characterized during the vegetative and generative phases including shoot length, harvest time, chlorophyll content, the number of productive tillers, the number of grain contents, the weight of 1,000 seeds, and the polymorphism profile using Random Amplified Polymorphic DNA Analysis (RAPD). The results of this study indicate that all radiation doses produce plants with random characters. Mutations using gamma rays at doses of 100 gy and 400 gy produced plants with better phenotypic characters than wild-type that is shorter plants, shorter harvest times, and more grain content. In addition, the results of the RAPD analysis confirm that there are genetic changes in irradiated rice. This mutants has the potential to reproduce germplasm and to obtain the next generation of mutants that have higher productivity and shorter plant heights.
Ethyl Methane Sulfonate (EMS) Effect on Mutagenesis in Balinese Red Rice (Oryza sativa cv. Barak Cenana) Ida Bagus Made Arta Dana; Popy Hartatie Hardjo; Maria Goretti Marianti Purwanto; Aloysia Sri Pujiyanti; Indriyani Indriyani
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2815

Abstract

“Barak Cenana” is one of the local red rice (Oryza sativa) with high economic value due to its nutrient content. Some of the agronomic characters such as tall shoot, low number of tiller, and late harvesting are drawbacks to improving production. Ethyl Methyl Sulphonate (EMS) is a chemical mutagen widely used to improve rice characters by mutation breeding. The present research aims to investigate the EMS concentration suitable to induce mutation in Barak Cenana and observe the mutagenesis effect of EMS on some agronomic characters of Barak Cenana. For those purposes, mature seeds of Barak Cenana were treated with various concentrations of EMS (0.25, 0.5, 0.75, 1.0, and 1.1 %) for 24 hours. Seed germination, plant growth, some agronomic character related to productivity were compared to untreated rice. EMS reduced and delayed seed germination and inhibit shoot and root growth in early vegetative stage. The ability of EMS to reduce and delay seed germination and inhibit shoot and root growth were doses dependent. EMS concentration of more than 1.0% was lethal for Barak cenana and EMS concentration at 0.75% was able to reduce rice productivity by increasing seed sterility. EMS concentrations between 0.25 and 0.5% with 24 h of soaking are suitable for inducing mutagenesis in Barak Cenana. Additionally, some mutants with distinct morphology such as sterile plant, semi-dwarf, high tillering capacity, and high chlorophyll contents were produced in this research.
TINJAUAN PUSTAKA: FERMENTASI DURIAN, TEMPOYAK SEBAGAI PANGAN FUNGSIONAL BERBASIS LOKAL Damayanti, Andi Alfina; Pujiyanti, Aloysia Sri; Dharma, Bodhi
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 17 No 1 (2025): BIOPROSPEK: Jurnal Ilmiah Biologi
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v17i1.1552

Abstract

Tempoyak merupakan produk fermentasi tradisional berbasis durian (Durio zibethinus) yang banyak dikonsumsi di Indonesia dan beberapa negara Asia Tenggara. Fermentasi tempoyak dilakukan secara spontan dengan dominasi bakteri asam laktat (BAL), seperti Lactobacillus plantarum, Streptococcus thermophilus, dan Leuconostoc mesenteroides. Kehadiran BAL berkontribusi pada peningkatan kualitas sensoris, keamanan pangan, serta manfaat fungsional tempoyak. Kajian ini bertujuan untuk meninjau potensi tempoyak sebagai pangan fungsional yange mengandung BAL sebagai agen probiotik dan antibakteri berdasarkan studi literatur terbaru. Hasil tinjauan pustaka menunjukkan bahwa metode fermentasi, suhu, dan kadar garam memengaruhi pertumbuhan BAL serta komposisi mikroba dalam tempoyak. Beberapa penelitian mengungkapkan bahwa BAL dalam tempoyak memiliki aktivitas antibakteri terhadap Escherichia coli dan Staphylococcus aureus, serta mampu menghasilkan senyawa bioaktif, seperti eksopolisakarida (EPS) yang berperan dalam meningkatkan daya tahan tubuh dan kesehatan pencernaan. Dengan karakteristik ini, tempoyak berpotensi sebagai pangan fungsional probiotik.
Allium spp. as a Source of Bioactive Antibacterials: A Review of Compounds, Mechanisms, and Effectiveness Hasibuan, Febry Rahmadhani; Pujiyanti, Aloysia Sri; Ilmi, Annisa Nurul; Hariani, Nova
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.10858

Abstract

Allium spp. has long been recognized for its diverse therapeutic benefits, particularly as a natural source of antibacterial agents. Various bioactive compounds found in Allium spp., such as allicin, diallyl disulfide, ajoene, and quercetin, have demonstrated significant antimicrobial activity against both gram-positive and gram-negative bacterial pathogens. Research on the mechanisms of antibacterial action of these compounds reveals that they work by disrupting bacterial cell membranes, inhibiting protein synthesis, and interfering with bacterial metabolism. This literature review aims to examine existing studies on the antibacterial potential of Allium spp., reviewing the bioactive compounds involved, their mechanisms of action, and their effectiveness in inhibiting bacterial growth, including antibiotic-resistant pathogens such as methicillin- resistant Staphylococcus aureus (MRSA) and Escherichia coli. The findings of this review indicate that while their effectiveness may vary depending on the specific compound and form of application, Allium spp. shows considerable promise as a natural alternative for combating bacterial infections and addressing the growing issue of antibiotic resistance. This article provides deeper insights into the relevance and potential of Allium spp. in the development of antimicrobial therapies based on natural sources through a literature-based approach.
Potensi Antioksidan Kombucha Bunga Telang (Clitoria ternatea) sebagai Minuman Fungsional Pujiyanti, Aloysia Sri; Ilmi, Annisa Nurul
Jurnal Ilmiah Pangan Halal Vol. 7 No. 2 (2025): Jurnal Ilmiah Pangan Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jiph.v7i2.19537

Abstract

Kombucha yang difermantasikan dari bunga telang (Clitoria ternatea) merupakan minuman fungsional dengan potensi antioksidan tinggi dan nilai tambah kesehatan. Beberapa penelitian melaporkan bahwa bunga telang kaya akan antosianin dan flavonoid, senyawa fenolik yang efektif menetralisir radikal bebas penyebab penyakit degeneratif seperti kanker, penyakit jantung, diabetes, dan penuaan dini serta mempertahankan mutu produk pangan. Metode tinjauan pustaka (literature review) menggunakan metode yang bersifat deskriptif-kualitatif dengan tujuan mengkaji potensi antioksidan kombucha berbasis bunga telang (Clitoria ternatea) sebagai minuman fungsional. Beberapa penelitian melaporkan aktivitas antioksidan yang diukur menggunakan metode DPPH pada kombucha bunga telang dengan rentang waktu fermentasi 8 hingga 20 hari menghasilkan aktivitas antioksidan yang tinggi dengan IC50 15%-19% dan % Inhibisi 70%-89%. Sehingga dapat disimpulkan dengan aktivitas antioksidan yang tinggi memiliki potensi sebagai minuman fungsional. Hasil review ini diharapkan dapat digunakan sebagai rujukan untuk penelitian lebih lanjut untuk mengetahui manfaat langsung dan kandungan dari kombucha bunga telang (Clitoria ternatea).
Ethnobotanical, Phytochemical, and Pharmacologies Aspect of Kaempferia rotunda L. as a Local Herbal Plant with Global Potential: A Review Pujiyanti, Aloysia Sri; Kusuma, Ratna; Ilmi, Annisa Nurul; Hasibuan, Febry Rahmadhani
Biocaster : Jurnal Kajian Biologi Vol. 5 No. 3 (2025): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v5i3.462

Abstract

Kaempferia rotunda L., also known as kunci pepet, is a local herbal plant with global potential traditionally used in various medicinal practices across Southeast Asia. This review summarizes the ethnobotanical, phytochemical, and pharmacological aspects of Kaempferia rotunda L., to explore its applications in modern medicine. Ethnobotanically, its rhizome has been widely utilized for treating digestive disorders, inflammation, and wound healing. Phytochemical studies reveal that Kaempferia rotunda L., contains flavonoids, alkaloids, phenols, and terpenoids, contributing to its biological activities. Several studies have demonstrated significant anti-inflammatory, antibacterial, antioxidant, and anticancer properties. With the increasing demand for natural-based therapies, further exploration of their active compounds and mechanisms of action is highly relevant. This review aims to provide a broader insight into the pharmaceutical potential of Kaempferia rotunda L., and encourage further research for the development of evidence-based herbal medicine.
Immunomodulatory and Antidiabetic Potential of Kratom (Mitragyna speciosa) on Animal Models: Potentials and Future Research Ilmi, Annisa Nurul; Hasibuan, Febry Rahmadhani; Pujiyanti, Aloysia Sri; Kurniati, Reni
Biocaster : Jurnal Kajian Biologi Vol. 5 No. 3 (2025): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v5i3.463

Abstract

Kratom (Mitragyna speciosa) has been traditionally used for its analgesic and stimulant properties. Recent preclinical studies suggesting its potential as an anti-inflammatory and antidiabetic agent. A comprehensive search of PubMed, ScienceDirect, and Google Scholar (2010-2024) using keywords “Kratom”, “inflammation”, and “diabetes” identified 82 studies. After applying inclusion criteria (original research, English/Bahasa Indonesia, in vivo animal models investigating anti-inflammatory/antidiabetic effects) and excluding reviews, in vitro studies, and conference abstracts, 14 studies comprising 9 key articles on the pharmacological activities of Kratom were analyzed. Dosages ranged from 0,5 mg/g to 500 mg/kg across various extraction methods and treatment durations. Studies show that Kratom extracts can inhibit pro-inflammatory cytokines (IL-1β, TNF-α) via NF-κB, COX-2, and MAPK/ERK pathways. In diabetic models, Kratom reduced blood glucose levels by 15-45%, improved glucose tolerance, and enhanced antioxidant status. Aqueous and ethanolic extracts showed comparable efficacy, with optimal effects observed at 100-200 mg/kg doses. Despite these findings, safety concerns remain regarding toxicity, dependence, and drug interactions. Further toxicological studies and clinical trials are essential to validate its therapeutic potential. This review highlights the need for comprehensive research and regulatory evaluation to support the safe medicinal use of Kratom.
KARAKTERISASI MORFOLOGI DAN ANALISIS LITERATUR PERAN VEKTOR KUTU PUTIH (PSEUDOCOCCIDAE) PADA TANAMAN HIAS DI LINGKUNGAN KAMPUS UNIVERSITAS MULAWARMAN: MORPHOLOGICAL CHARACTERISATION AND LITERATURE ANALYSIS OF THE ROLE OF WHITEFLY VECTORS (PSEUDOCOCCIDAE) ON ORNAMENTAL PLANTS IN THE CAMPUS ENVIRONMENT OF MULAWARMAN UNIVERSITY Hasibuan, Febry Rahmadhani; Khotizah, Laraz Nur; Butar, Marentina Kirana Butar; Hariyanti, Mutina; Putri, Rania Sakanti Arindra; Veraresa, Ray Herry; Widya Syahfitri; Ilmi, Annisa Nurul; Pujiyanti, Aloysia Sri
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2025.013.3.2

Abstract

Kutu putih (Pseudococcidae) merupakan hama penting yang menyebabkan kerusakan fisiologis dengan mengisap getah dari floem dan juga bertindak sebagai vektor patogen tumbuhan, termasuk virus, bakteri, dan jamur. Tujuan studi ini adalah untuk mengidentifikasi spesies kutu putih pada tanaman hias dan mengevaluasi potensi mereka dalam menularkan patogen di lingkungan tropis. Sampel dikumpulkan dari tiga spesies tanaman hias, yaitu Agave americana, Heptapleurum arboricola, dan Colocasia esculenta L, di Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) Universitas Mulawarman. Penelitian ini menggunakan desain deskriptif-eksploratif untuk mengidentifikasi spesies kutu putih dan menganalisis potensinya sebagai vektor patogen pada tanaman hias.  Pengambilan sampel dilakukan secara purposif dengan kriteria inklusi sebagai berikut: (1) tanaman hias yang menunjukkan gejala infestasi kutu putih (misalnya koloni berwarna putih, daun menguning, atau nekrosis), (2) tanaman yang termasuk dalam tiga spesies target (Agave americana, Heptapleurum arboricola, dan Colocasia esculenta L.), dan (3) lokasi tumbuh yang terpapar kondisi lingkungan alami (tidak dalam rumah kaca atau perlakuan pestisida). Identifikasi morfologis menunjukkan dua spesies kutu putih dominan, Paracoccus marginatus dan Pseudococcus cryptus, yang ditemukan pada fase nimfa betina dan dewasa. Fase instar II dan III melibatkan aktivitas makan dan pengeluaran madu, yang memfasilitasi penularan patogen melalui mekanisme mekanis dan biologis. Pengeluaran ini juga menciptakan kondisi ideal untuk kolonisasi patogen sekunder, seperti spesies Fusarium dan Capnodium. Kondisi iklim tropis yang hangat dan lembap memperkuat vektorisasi patogen oleh kutu putih. Temuan ini memperkuat gagasan bahwa kutu putih memainkan peran epidemiologis yang signifikan dalam penyebaran penyakit tanaman hias, memberikan dasar penting untuk strategi pengendalian biologis dan pemantauan penyakit di lingkungan tropis.
Co-Authors Bintang Kerta Wijaya Butar, Marentina Kirana Butar Damayanti, Andi Alfina Dharma, Bodhi Febry Rahmadhani Hasibuan Hariyanti, Mutina Ida Bagus Made Arta Dana Ida Bagus Made Artadana Ilmi, Annisa Nurul Indriyani Indriyani Khotizah, Laraz Nur Kurniati, Reni Maria Goretti Marianti Purwanto Nova Hariani Popy Hartatie Hardjo Putri, Rania Sakanti Arindra Ratna Kusuma, Ratna Veraresa, Ray Herry Widya Syahfitri