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All Journal Jurnal Medik Veteriner ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal
Lesmana, Muhamad Arfan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

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Detection of Staphylococcus aureus Biofilm from Subclinical Mastitis Milk Lesmana, Muhamad Arfan; Qosimah, Dahliatul; Murwani, Sri
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.132 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.01.3

Abstract

One of S.aureus's virulence factors is biofilm formation. When biofilms are formed, the bacteria will undergo phenotypic changes that require higher concentrations of antibiotics to inhibit proliferation. Phenotypic changes will lead to increase the production of extracellular matrix and multilayered colonies as well as decrease of metabolic rates, multiplication and polymicrobial colonization resulting in recurrent infection in the host and difficulty being treated with antibiotics due to resistance. The aim of this research was to know the formation of bacterial biofim by slime and quantitative by microplate titer method. The research method was qualitative descriptive using 27 samples of Staphylococcus aureus  with characterized from mastitis infected milk. The bacteria were grown on CRA (Congo Red Agar) media to observe the slime biofilm through bacteria black colony followed by MicrotiterPlate  method  with 570nm wave lenght. The results showed that 27 samples of Staphylococcus aureus which positive to form slime biofilm were 10 samples and continued to microtiter plate showed 3 positive samples of biofilm. The conclusions of this study, Staphylococcus aureus in subclinical mastitis milk samples were positive to form biofilms. 
Surgical Procedure for Pyometra and Mammae Tumor Treatment in a Pitbull Dog Rickyawan, Nofan; Virgiantari, Cheptien Winda; Lesmana, Muhamad Arfan; Vidiastuti, Dian
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.109-118

Abstract

Pyometra is an infection or inflammation of the uterine wall characterized by the accumulation of pus in the uterine lumen. Predisposing factors include age, breed, bacterial infection, and hormones. Another disease that is often correlated with an imbalance in reproductive hormones is mammary tumors. A 7-year-old unspayed female Pitbull dog weighed 27.9 kg has clinical symptoms of frequent discharge of mucopurulent reddish-brown discharge with a foul odor from the vagina. The left mammary glands of the second, third, and fourth nipples have lumps about 20 cm in diameter, are solid in consistency, well-defined, the same color as the skin surface, and can be moved. Diagnosis performed on physical examination, hematology, blood chemistry, and cytology was pyometra and mammary tumors. Treatment was done by ovariohysterectomy and unilateral mastectomy. Post-surgery therapy was enrofloxacin 5 mg/kg, ketoprofen 2 mg/kg, phytomenadione 1 mg/kg, and Biodin® 0.1 ml/kg, followed by intensive surgical wound care. The patient recovered after two months of treatment.
Cystotomy kasus urolithiasis pada anjing pomeranian Lailiyyah, Rintik Nangiimatul; Indriastuti, Feny; Lesmana, Muhamad Arfan
ARSHI Veterinary Letters Vol. 6 No. 1 (2022): ARSHI Veterinary Letters - Februari 2022
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.6.1.5-6

Abstract

Urolithiasis merupakan adanya pembentukan sedimen di dalam saluran perkencingan yang terdiri dari satu atau lebih kristaloid urin yang sukar larut. Tulisan ini melaporkan proses penanganan melalui cystotomy kasus urolithiasis pada anjing pomeranian di Klinik Hewan Drh. Feny Indriastuti Purwokerto. Anjing betina ras pomeranian dengan bobot badan 2,9 kg menunjukkan respon sakit saat dilakukan palpasi pada regio hipogastrik. Anjing mengalami hematuria dan stranguria selama setahun terakhir. Hasil pencitraan ultrasonografi didapatkan massa hiperekoik pada vesika urinaria. Hasil pemeriksaan hematologi menunjukkan anjing mengalami leukositosis. Tindakan yang dilakukan pada anjing adalah cystotomy. Urolit yang dikeluarkan berukuran 3,5 cm. Anjing disarankan diberikan diet pakan khusus untuk gangguan urinari pascabedah.
Co-Authors Dian Vidiastuti, Dian Indriastuti, Feny Lailiyyah, Rintik Nangiimatul Qosimah, Dahliatul Rickyawan, Nofan Sri Murwani Virgiantari, Cheptien Winda