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All Journal Majalah Kedokteran Sriwijaya Biomedical Journal of Indonesia Bioscientia Medicina : Journal of Biomedicine and Translational Research Bioscientia Medicina : Journal of Biomedicine and Translational Research
Nova Kurniati
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Sekresi IFN-γ dan IL-10 Setelah Stimulasi Antigen Fusi ESAT-6-CFP-10 (EC610) pada Penderita TB Aktif dan TB Laten Sabrina Prihantika; Nova Kurniati; Kemas Ya’kub Rahadiyanto; M. Irsan Saleh; Zen Hafy; Francisca Srioetami Tanoerahardjo; Jusak Nugraha; Eddy Mart Salim
Biomedical Journal of Indonesia Vol. 5 No. 3 (2019): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Abstract

Sistem imunitas seluler sangat berperan dalam melawan infeksi TB yaitu peran sel limfosit T yang berdiferensiasimenjadi sel Th1 mensekresikan sitokin pro inflamasi IFN-γ dan sel Th2 yang mensekresi sitokin anti inflamasi IL-10.Antigen fusi EC610 bersifat spesifik dan memiliki antigenitas yang kuat terhadap stimulasi sel T. Tujuan penelitian untukmengetahui perbedaan rerata kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610 pada penderita TB aktif dan TBlaten. Desain penelitian ini adalah eksperimen semu secara in vitro dengan kultur PBMC yang distimulasi oleh antigenEC610 pada kelompok TB aktif dan TB laten. Penelitian dan pemeriksaan dilakukan di RSK Paru Provinsi Sumsel danLaboratorium Pusat Penelitian dan Pengembangan Kesehatan, Jakarta Pusat. Terdapat 21 subjek penderita TB aktif dan28 subjek penderita TB laten yang sesuai dengan kriteria inklusi. Pengukuran kadar IFN-γ dan IL-10 dilakukanmenggunakan ELISA-Reader. Hasil penelitian menunjukkan kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610lebih tinggi pada TB aktif daripada TB laten. Tingginya kadar IFN-γ pada penderita TB aktif menunjukkan adanyarespon imun protektif terhadap kuman M.tb sedangkan tingginya kadar IL-10 menunjukkan perannya sebagai antiinflamasi . Tidak terdapat perbedaan bermakna rerata kadar IFN-γ pada penderita TB aktif dan TB laten (p=0,769) danterdapat perbedaan bermakna reata kadar IL-10 setelah stimulasi antigen EC610 lebih tinggi pada TB aktif daripada TBlaten (p=0,000).
IFN-γ and IL-2 Secretion after ESAT-6-CFP-10 (EC-610) Fusion Antigen Stimulation from Patients with Active Lung Tuberculosis and Latent Lung Tuberculosis Bastian; Nova Kurniati; Kemas Ya’kub Rahadiyanto; M. Irsan Saleh; Zen Hafy; Francisca Srioetami Tanoerahardjo; Jusak Nugraha; Eddy Mart Salim
Biomedical Journal of Indonesia Vol. 6 No. 2 (2020): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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The Secretion of IFN-γ and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Activeand Latent TB Patients. This study held to discover how immune responses work and to know thepathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells withESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-γ and IL-2 with ELISA antibodysandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube withQuantiFERON for IFN-γ assay. The other four tubes were PBMC isolated using Ficoll-Paque, andpre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C andmeasured using T-Spot and ELISA reader. We got from this study that there are no significantdifferences in IFN-γ levels for both groups with active TB and latent TB. Measurement of IL-2 levelsshowed significant differences between the two group.
Hubungan antara Tingkat Aktivitas Penyakit LES dan Tingkat Depresi pada Penderita Lupus Eritematosus Sistemik di Persatuan Lupus Sumatera Selatan dan Poliklinik Ilmu Penyakit Dalam Annisa Istiqomah; Nova Kurniati; Phey Liana
Majalah Kedokteran Sriwijaya Vol 50, No 4 (2018): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v50i4.8566

Abstract

Depresi merupakan salah satu manifestasi klinis yang dapat muncul pada penderita LES. Diduga tingkat aktivitas penyakit LES dapat mempengaruhi kejadian depresi tersebut. Penelitian ini bertujuan untuk meneliti hubungan antara tingkat aktivitas penyakit LES dan tingkat depresi pada penderita Lupus Eritematosus Sistemik di Persatuan Lupus Sumatera Selatan dan Poliklinik Ilmu Penyakit Dalam RSUP Dr. Mohammad Hoesin Palembang. Penelitian ini merupakan penelitian analitik observasional dengan desain studi cross sectional. Penelitian dilaksanakan pada bulan September 2017 sampai dengan November 2017 di Persatuan Lupus Sumatera Selatan dan Poliklinik Ilmu Penyakit Dalam RSUP Dr. Mohammad Hoesin Palembang. Diambil sampel sebesar 42 orang penderita LES dengan metode consecutive sampling. Data diambil dari pengisian kuesioner MEX-SLEDAI dan BDI serta wawancara terhadap responden penelitian. Selain itu, diambil juga data rekam medik pasien untuk melengkapi data penelitian. Uji statistik menggunakan uji One Way ANOVA. Terdapat hubungan yang bermakna (p value = 0,000) antara tingkat aktivitas penyakit LES dan tingkat depresi pada penderita LES di Persatuan Lupus Sumatera Selatan dan Poliklinik Ilmu Penyakit Dalam RSUP Dr. Mohammad Hoesin Palembang. Hubungan masing-masing kategori dari variabel penelitian, yaitu tingkat aktivitas LES ringan: 80% tidak depresi, 20% depresi ringan; tingkat aktivitas LES sedang: 44,4% tidak mengalami depresi, 33,3% depresi ringan, 22,2% depresi sedang; tingkat aktivitas LES berat: 7,1% tidak mengalami depresi, 7,1% depresi ringan, 21,4% depresi sedang, dan 64,3% depresi berat. Semakin tinggi tingkat aktivitas penyakit LES maka semakin tinggi pula tingkat depresi yang dialaminya.
A Rare Case of Antithyroid Drug-Induced Lupus Erythematosus, Graves' Disease, and Primary Infertility Kusuma, Singgih; Edi Susanto; Nova Kurniati; RM Dewi Anggraini
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 1 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i1.1177

Abstract

Background: Drug-induced lupus erythematosus (DILE) is a rare autoimmune disorder that mimics idiopathic lupus erythematosus, triggered by certain medications. This case report presents a patient with DILE induced by methimazole, a commonly used antithyroid drug, along with Graves' disease and primary infertility. Case presentation: A 41-year-old woman presented with palpitations, a history of Graves' disease treated with methimazole, and primary infertility. She developed lupus-like symptoms including fever, joint pain, and skin rash. Examination revealed tachycardia, tenderness of the right and left knee joints and limited range of motion. Laboratory investigations confirmed hyperthyroidism and autoimmune features consistent with DILE. Conclusion: This case highlights the rare occurrence of DILE induced by methimazole, emphasizing the importance of recognizing and managing this condition in patients receiving antithyroid drugs.
Neutrophil-Lymphocyte Ratio as a Novel Biomarker for Diabetic Nephropathy in Type 2 Diabetes Mellitus Patients: A Cross-Sectional Study Pradesta, Rahnowi; Novadian; Yulianto Kusnadi; Nova Kurniati; Syarif Husin
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 3 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i3.1235

Abstract

Background: Early detection of diabetic nephropathy (DN) is crucial to prevent progression to end-stage renal disease. The gold standard for diagnosing DN involves urine microalbumin testing and renal biopsy. However, the availability of these diagnostic tools is limited in many healthcare facilities across Indonesia. Consequently, there is a pressing need for an alternative examination that is readily accessible and can effectively monitor the progression of DN. Methods: This cross-sectional study was conducted at Dr. Mohammad Hoesin General Hospital, Palembang, from February 2024 to May 2024. The study aimed to investigate the correlation between neutrophil-lymphocyte ratio (NLR) and urinary albumin levels in type 2 diabetes mellitus (DM) patients. NLR, calculated from complete blood counts, has emerged as a potential inflammatory marker for various conditions. A total of 65 participants diagnosed with type 2 DM were enrolled in the study. Data analysis involved Spearman's correlation test to assess the relationship between NLR and urinary albumin levels. Results: The majority of the 65 subjects were female (58.5%). The study found that 44 subjects had normoalbuminuria, 18 had microalbuminuria, and 3 had macroalbuminuria. A significant positive correlation was observed between NLR and albuminuria levels in type 2 DM patients (r = 0.795; p < 0.01). Conclusion: The study's findings suggest that NLR is a potential cost-effective biomarker for the early detection of DN in type 2 DM patients, especially in resource-limited settings. Further large-scale studies are recommended to validate these findings and establish specific NLR thresholds for predicting DN progression.
Systemic Inflammatory Immune Index (SII) Predicts Disease Activity in Systemic Lupus Erythematosus (SLE) Patients: A Cross-Sectional Study Susanto, Edi; Yuniza; Legiran; Nova Kurniati; Eddy Mart Salim
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 4 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i4.1237

Abstract

Background: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by widespread inflammation and diverse clinical manifestations. The systemic inflammatory immune index (SII), calculated as platelet count * neutrophil count/lymphocyte count, has emerged as a potential marker of systemic inflammation in various conditions. This study aimed to investigate the relationship between SII and disease activity in SLE patients. Methods: We conducted a cross-sectional study involving 60 SLE patients diagnosed according to the 2019 EULAR/ACR classification criteria. Demographic and clinical data were collected, and disease activity was assessed using the Mexican Systemic Lupus Erythematosus Disease Activity Index (MEX-SLEDAI). Blood samples were analyzed to determine SII values. Statistical analysis included Spearman's correlation to assess the relationship between SII and MEX-SLEDAI scores. Results: The study population predominantly consisted of women (98.3%), with a median age of 29 years. A strong positive correlation was observed between SII and MEX-SLEDAI scores (r = 0.931, p < 0.001). Patients with higher SII values exhibited significantly greater disease activity. Conclusion: Our findings suggest that SII is a promising predictor of disease activity in SLE patients. This readily available index may aid clinicians in assessing disease severity and tailoring treatment strategies. Further research is warranted to validate these findings and explore the utility of SII in monitoring disease progression and treatment response.
Immunomodulatory Effects of Mesenchymal Stem Cell Secretome in Systemic Lupus Erythematosus: A Meta-Analysis Narisa Felinka Kusuma; Nova Kurniati
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 4 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i4.1244

Abstract

Background: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by immune system dysregulation and multi-organ damage. Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic option due to their immunomodulatory properties, primarily mediated through their secretome (MSCS). This meta-analysis aimed to evaluate the efficacy and safety of MSCS in SLE patients. Methods: A systematic search of PubMed, Embase, and Web of Science was conducted for studies published between 2013 and 2024 investigating the effects of MSCS in SLE. Randomized controlled trials (RCTs) comparing MSCS with placebo or standard care were included. The primary outcome was SLE disease activity, assessed using the SLE Disease Activity Index (SLEDAI). Secondary outcomes included immunological markers (e.g., anti-dsDNA antibodies, complement levels), quality of life, and adverse events. Data were pooled using a random-effects model. Results: Nine RCTs (n=485 patients) met the inclusion criteria. MSCS therapy significantly reduced SLEDAI scores compared to controls (standardized mean difference [SMD] -0.78, 95% CI -1.25 to -0.31, p=0.001). Significant improvements were also observed in anti-dsDNA antibody levels (SMD -0.62, 95% CI -1.01 to -0.23, p=0.002) and complement C3 levels (SMD 0.55, 95% CI 0.21 to 0.89, p=0.002). MSCS was generally well-tolerated, with no serious adverse events reported. Conclusion: This meta-analysis demonstrates that MSCS therapy has significant immunomodulatory effects in SLE, leading to improved disease activity and immunological profiles. Larger, well-designed RCTs with longer follow-up periods are needed to confirm these findings and assess the long-term efficacy and safety of MSCS in SLE.
The Paradoxical Role of Interleukin-10 in Systemic Lupus Erythematosus: A Correlational Study of Serum Levels and Disease Activity Yuniza; Joneri, Alrahman; Legiran; Nova Kurniati; Eddy Mart Salim
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 12 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i12.1452

Abstract

Background: Systemic lupus erythematosus (SLE) is a multifaceted autoimmune disease where the cytokine Interleukin-10 (IL-10) exhibits a paradoxical role, functioning as both a potent anti-inflammatory mediator and a robust B-cell stimulator. The clinical significance of serum IL-10 as a biomarker of disease activity is a subject of intense debate, with conflicting reports in the literature. This study was designed to investigate this relationship within a specific Southeast Asian cohort. Methods: An observational, cross-sectional study was conducted at a tertiary referral hospital in Palembang, Indonesia, enrolling 48 adult patients with a confirmed diagnosis of SLE. Disease activity was quantitatively scored using the Mexican Systemic Lupus Erythematosus Disease Activity Index (MEX-SLEDAI). Serum IL-10 concentrations were precisely measured using a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The primary statistical analysis involved the Spearman rank correlation test. A post-hoc power analysis was performed to contextualize the statistical findings. Results: The study population was predominantly female (95.8%), with the largest subgroup (54.2%) presenting with mild disease activity. The mean serum IL-10 concentration was 9.91±1.36 pg/mL in the mild activity group, rose to a peak of 12.22±1.95 pg/mL in the moderate activity group, and was 10.65±2.34 pg/mL in the severe activity group. The Spearman correlation test identified a weak, positive association that did not achieve statistical significance (r=0.274, p=0.059). The post-hoc power analysis confirmed the study was underpowered to definitively detect a correlation of this magnitude. Conclusion: In this cohort of Indonesian SLE patients, a statistically significant correlation between serum IL-10 levels and disease activity was not established. Given the study's methodological context, including its cross-sectional design and limited statistical power, the findings are inconclusive but hypothesis-generating. The results reinforce the profound complexity of IL-10 biology in SLE and underscore the challenges in validating it as a standalone biomarker, highlighting the need for larger, longitudinal investigations.
The Paradoxical Role of Interleukin-10 in Systemic Lupus Erythematosus: A Correlational Study of Serum Levels and Disease Activity Yuniza; Joneri, Alrahman; Legiran; Nova Kurniati; Eddy Mart Salim
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 12 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i12.1452

Abstract

Background: Systemic lupus erythematosus (SLE) is a multifaceted autoimmune disease where the cytokine Interleukin-10 (IL-10) exhibits a paradoxical role, functioning as both a potent anti-inflammatory mediator and a robust B-cell stimulator. The clinical significance of serum IL-10 as a biomarker of disease activity is a subject of intense debate, with conflicting reports in the literature. This study was designed to investigate this relationship within a specific Southeast Asian cohort. Methods: An observational, cross-sectional study was conducted at a tertiary referral hospital in Palembang, Indonesia, enrolling 48 adult patients with a confirmed diagnosis of SLE. Disease activity was quantitatively scored using the Mexican Systemic Lupus Erythematosus Disease Activity Index (MEX-SLEDAI). Serum IL-10 concentrations were precisely measured using a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The primary statistical analysis involved the Spearman rank correlation test. A post-hoc power analysis was performed to contextualize the statistical findings. Results: The study population was predominantly female (95.8%), with the largest subgroup (54.2%) presenting with mild disease activity. The mean serum IL-10 concentration was 9.91±1.36 pg/mL in the mild activity group, rose to a peak of 12.22±1.95 pg/mL in the moderate activity group, and was 10.65±2.34 pg/mL in the severe activity group. The Spearman correlation test identified a weak, positive association that did not achieve statistical significance (r=0.274, p=0.059). The post-hoc power analysis confirmed the study was underpowered to definitively detect a correlation of this magnitude. Conclusion: In this cohort of Indonesian SLE patients, a statistically significant correlation between serum IL-10 levels and disease activity was not established. Given the study's methodological context, including its cross-sectional design and limited statistical power, the findings are inconclusive but hypothesis-generating. The results reinforce the profound complexity of IL-10 biology in SLE and underscore the challenges in validating it as a standalone biomarker, highlighting the need for larger, longitudinal investigations.
Co-Authors Bastian Eddy Mart Salim Edi Susanto Faisal, Raden Muhammad Francisca Srioetami Tanoerahardjo Joneri, Alrahman Jusak Nugraha Kemas Ya’kub Rahadiyanto Kusuma, Singgih Laksono Trisnantoro Legiran Legiran M. Irsan Saleh Narisa Felinka Kusuma Novadian Phey Liana Pinto Desti Ramadhoni Pradesta, Rahnowi Riswandi, Asep RM Dewi Anggraini Sabrina Prihantika Syarif Husin Yulianto kusnadi Yuniza Zen Hafy