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All Journal Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry
Shariff, Khairul Anuar
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Viability of 7F2 pre-osteoblast after Sulawesi stingless bee (Tetragonula biroi) 1% propolis nanoemulsion extraction Sitalaksmi, Ratri Maya; Amalia, Nadya Rafika; Nugraha, Alexander Patera; Budhy, Theresia Indah; Ramadhani, Nastiti Faradilla; Situmorang, Putri Cahaya; Noor, Tengku Natasha Eleena binti Tengku Ahmad; Shariff, Khairul Anuar
Majalah Kedokteran Gigi Indonesia Vol 10, No 2 (2024): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.98860

Abstract

Produced by a stingless bee (Tetragonula biroi) from Sulawesi Island, propolis is an active compound that requires further studies to determine its herbal-based medicinal applications. In addition, propolis is well known as a potential natural ingredient for herbal medicine with minimal side effects. The stingless honeybee, native to Sulawesi, produces propolis, a resin. Propolis from Trigona stingless bees in Sulawesi has the highest flavonoid content compared to Apis mellifera bees. Flavonoids are the most common compounds contained in propolis. Propolis from stingless bees may have the potential to stimulate osteoblast cell proliferation and be responsible for bone regeneration. The objective of this study is to investigate the viability of the 7F2 pre-osteoblast cell line after administration of the stingless bee’s 1% propolis nanoemulsion extract (PNE). A 1% PNE was formulated by maceration methods and diluted into several concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78%. A particle size analysis was done to examine the particle size and zeta potential of the 1% PNE. A Tetrazolium Technique assay was carried out to examine 1% PNE application on a 7F2 pre-osteoblast cell line on days 1, 3, and 7. The highest viability of 7F2 pre-osteoblast cells was at 0.78% concentration on day 7, and the lowest viability was observed at 100% concentration on day 1, with significant differences among groups (p < 0.05). A 1% PNE with a particle size of 151.28 to 182.2 nm and a zeta potential of −32.76 mV was successfully formulated. The 1% PNE was non-toxic to 7F2 pre-osteoblast cells, with a cell toxicity limit at a concentration of 1.56%.
Degradation of chitosan–gelatin and chitosan–gelatin–β-tricalcium phosphate scaffolds Putri, Tansza Setiana; Pratiwi, Deviyanti; Margaretta, Dewi Liliany; Tjandrawinata, Rosalina; Shariff, Khairul Anuar
Dental Journal (Majalah Kedokteran Gigi) Vol. 57 No. 2 (2024): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i2.p87-90

Abstract

Background: Fabrication of the composite scaffold was carried out by combining chitosan, gelatin, and β-tricalcium phosphate (βTCP) derived from limestone. The extraction of βTCP was based on the abundance of limestone containing calcium carbonate, which can be a source of βTCP synthesis. Purpose: This study evaluates the degradation of the combination of chitosan–gelatin (ChG) and chitosan–gelatin–βTCP (ChG-βTCP) composite scaffolds. Methods: The freeze-drying method was used to obtain the composite scaffold, which was a mixture of chitosan, gelatin, and βTCP. Degradation was measured by immersing the samples in a simulated body fluid solution at 37°C for 3, 7, 14, and 21 days. For statistical analysis, one-way analysis of variance (ANOVA) and post hoc Fisher's least significant difference were performed. Results: The ChG scaffold shows better degradability than the ChG-βTCP scaffold. The ChG scaffold shows higher weight degradation than the ChG-βTCP scaffold up to 21 days. Conclusion: In conclusion, the scaffold containing βTCP has lower degradation than the ChG scaffold.
Comparison of shear bond strength of etch and rinse, self-etch adhesive system followed by flowable composite resin, and self-adherent composite resin Zakaria, Myrna Nurlatifah; Suri, Yuyun Andina; Soerachman, Badi; Shariff, Khairul Anuar; Cahyanto, Arief
Padjadjaran Journal of Dentistry Vol 32, No 3 (2020): November 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no3.27989

Abstract

Introduction: The adhesive system strongly influences the bond strength of resin composite to the tooth surface. Aside from the commonly used etch and rinse, and self-etch adhesive system and a new approach called self-adherent composite resin to combine the adhesive system to the composite material. This study was aimed to compare the shear bond strength of two adhesive systems, (etch and rinse, self-etch adhesive system) followed by application of a flowable composite resin to a self-adherent resin composite. Methods: This study was experimental consisted of three groups of treatment, G1 (etch and rinse adhesive system+composite resin); G2 (self-etch adhesive system+composite resin), and G3 (self-adherent composite resin). Samples were 27 dentinal surfaces from premolar teeth bonded to composite resin (3 mm diameter and 3 mm in height), according to ISO technical specification No 11405. Shear bond strength test was performed using Universal Testing Machine (UTM) under the load of 50 kgf, and the crosshead speed of 0.5 mm/min, and the data was analysed by one-way ANOVA afterwards. Results: The highest bond strength to dentin was seen in Group 1 (14.89 MPa) followed by Group 2 (11.65 MPa) and Group 3 (11.22 MPa) with no significant difference between the three groups p value =0.117 (p>0.05). Conclusion: The etch and rinse adhesive system had the highest shear bond strength to dentin, followed by the self-etch and the self-adherent composite resin. However, the shear bond strength of all tested groups was comparable.
Co-Authors Alexander Patera Nugraha Amalia, Nadya Rafika Badi Soerachman, Badi Cahyanto, Arief Deviyanti Pratiwi Dewi Liliany Margaretta, Dewi Liliany Myrna Nurlatifah Zakaria, Myrna Nurlatifah Nastiti Faradilla Ramadhani Noor, Tengku Natasha Eleena binti Tengku Ahmad Ratri Maya Sitalaksmi Rosalina Tjandrawinata Suri, Yuyun Andina Tansza Setiana Putri Theresia Indah Budhy Sulisetyawati