Articles
<![CDATA[THE POTENTIAL OF HEDYOTIS CORYMBOSA (L.) LAMK EXTRACT TO INHIBIT THE PROGRESSIVITY OF ORAL CANCER CELL IN RATS INDUCED WITH BENZOPYRENE ]]>
<![CDATA[Budhy, Theresia Indah]]>
<![CDATA[ Dentika: Dental Journal Vol. 19 No. 1 (2016): Dentika Dental Journal ]]>
Publisher : <![CDATA[TALENTA ]]>
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DOI: 10.32734/dentika.v19i1.142
<![CDATA[Cancer is still ranked as the fifth cause of mortality and morbidityin Indonesia.Hedyotis corymbosa (L.) Lamk has ursolat acid as anti-proliferative cancer cell. This research is aimed to determine the potency of Hedyotis corymbosa (L.) Lamk at different doses, namely 375, 750, and 1500 mg/kg, used as an inhibitor for the progressivity of oral cancer, such as proliferation, angiogenesis, and apoptosis of cancer cells. Post test only control group design was used in this research. There were 24 Rattus novergicus used as research samples. Those were divided into four groups, namely control, treatment group 1 with a dose of 375mg/kg, treatment group 2 with a dose of 750 mg/kg, and treatment group 3 with a dose of 1500mg/kg. Their oral cavity was induced intramusculary by benzopyrene with a dose of 8mg/kg for 4 weeks (twice a week) to create cancer. Hedyotis corymbosa (L.) Lamk was given orally for 10 days. All samples were aclimatitation to perform Histo Pathology Anatomi among groups. Haematoxillin Eosin for proliferation cancer cell and capilary. Immunohistochemistry for expression of caspase3. Data were tabulated andanalyzed statistically by ANOVA. There was significant difference of cancer cell proliferation and capilary between control and treatment groups. The most significant decreasing of cancer cell proliferation was in those samples given with a dose of 750 mg/kg. Meanwhile, the highest apoptosis of caspase3 expression was in those samples given with a dose of 750 mg/kg. It can be concluded that Hedyotis corymbosa (L.) Lamk extract could decrease cancer cell proliferation and capilary as well as could increase apoptosis.]]>
<![CDATA[Homesickness, Anxiety and Depression among Pakistani International Students in Indonesia during Covid-19 Outbreak ]]>
<![CDATA[Shahzad Shoukat]]>;
<![CDATA[Cyuzuzo Callixte]]>;
<![CDATA[Theresia Indah Budhy]]>;
<![CDATA[Jusak Nugraha]]>;
<![CDATA[Tuyishimire Irene]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i4.17077
<![CDATA[Introduction: International students are exposed to multiple mental health crisis due to social, environmentaland culture shocks in foreign lands but it gets worse in presence of life- threatening disease outbreak. Thisresearch was conducted to evaluate the homesickness, anxiety and depression among Pakistani internationalstudents in Indonesia during Covid-19 Outbreak. Methods: This study was conducted on 86 randomstudents that are pursuing their studies in different public and private universities in Indonesia. The datawere collected by distributing google forms via email and social media groups and the responses fromthe respondents were recorded and analyzed statistically. Results: The study findings revealed that thereis a correlation between Covid-19 pandemic and the development of various mental health crisis where33 students (38.37 %) developed homesickness, 11 students (12.79%) developed anxiety and 2 students(2.33 %) manifested low-grade depression. Homesickness was outstandingly observed in females (72.72%) whereas anxiety was highly reported in males (90.90%).The study findings demonstrated that there isa strong negative correlation between student financial statuses and the development of homesickness (r:-0.977, P: 0.023) and anxiety (r: -0.944, P: 0.056).Conclusion: All in all, the fear to contract coronavirus,lockdowns, financial instability, death toll of citizens and medical professionals, run out of medical facilitiesand social media hoaxes are significant risk factors of mental health crisis among Pakistani internationalstudents in Indonesia.]]>
<![CDATA[Identifikasi Kromatografi Lapis Tipis Sudamala (Artemisia vulgaris L.) ]]>
<![CDATA[Ira Arundina]]>;
<![CDATA[Theresia Indah Budhy S.]]>;
<![CDATA[Muhammad Luthfi]]>;
<![CDATA[Retno Indrawati]]>
<![CDATA[ Majalah Kedokteran Gigi Indonesia Vol 1, No 2 (2015): December ]]>
Publisher : <![CDATA[Faculty of Dentistry, Universitas Gadjah Mada ]]>
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DOI: 10.22146/majkedgiind.9226
<![CDATA[Identification of Sudamala (Artemisia vulgaris L.) Thin Layer Chromatography. Oral squamous cell carcinoma is the type of cancer which is most frequently found in oral cavity. The primary risk factor of malignancy in oral cavity includes the habit of consuming tobacco and or alcohol. The plant sudamala (Artemisia vulgaris L.) is often used in the community as anti-tumor in digestive organ, including in oral cavity. However, there have been no studies on active ingredients playing the role as anti-cancer in oral cavity. The species are mostly from the genus Artemisia, while those generally growing in Indonesia are the species Artemisia vulgaris L. The objective of this study is to explain the identification by TLC of sudamala. The study was sudamala extraction, identification of sudamala extract, sudamala fractionation with Vacuum Column Chromatography and identification of sudamala fractionation with TLC (Thin Layer Chromatography). The result shows that the fractionation using hexane extract resulted in 11 fractions of n-hexane: ethyl acetate. The conclusion of this study is that the fraction of n-hexane: ethyl acetate (3:7, v/v) sudamala (Artemisia vulgaris L.) identified contains terpenoids compounds.]]>
<![CDATA[The ability of 5% Tamarindus indica extract as cleaner of the root canal wall smear layer ]]>
<![CDATA[Erawati Wulandari]]>;
<![CDATA[Latief Mooduto]]>;
<![CDATA[Theresia Indah Budhy S]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 4 (2007): December 2007 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
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DOI: 10.20473/j.djmkg.v40.i4.p173-175
<![CDATA[Tamarindus indica is one of traditional medicines. Pulpa tamaridorum consist of organic acid that is usually used as irrigant and to remove root canal wall smear layer. The aimed of this study was to elucidate the ability of 5% Tamarindus indica extract as a root canal irrigant to remove root canal wall smear layer. Eighteen tooth samples were cut on cervical line and divided into 2 then groups were prepared with K file and irrigated. Group 1 was irrigated by sterile aquabidest and group 2 was irrigated by 5% Tamarindus indica extract. Samples were cut longitudinally and formed 7 × 2× 2 mm specimen. Each specimen was photographed by scanning electron microscope, scored and summed. The total score obtained is used as the hygiene value of root canal wall. The collected data were statistically analyzed by using independent t test at 0.05 level. The result of the study showed there was a significant difference between 5% Tamarindus indica extract and sterile aquabidest (p < 0.05), the hygiene value of 5% Tamarindus indica extract was higher than sterile aquabidest. The conclusion of this investigation showed that 5% Tamarindus indica extract remove root canal wall smear layer.]]>
<![CDATA[The effect of Hegu acupoint stimulation in dental acupuncture analgesia ]]>
<![CDATA[Fransiskus Andrianto]]>;
<![CDATA[Jenny Sunariani]]>;
<![CDATA[Theresia Indah Budhy S]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 1 (2007): March 2007 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
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DOI: 10.20473/j.djmkg.v40.i1.p37-41
<![CDATA[In daily life, dental treatments are often related with oral pain sensation which needs anesthesia procedures. Sometimes local anesthetics can not be used because patients have hypersensitive reaction or systemic diseases which may lead to complications. Stimulating acupoint, such as Hegu activates hypothalamus and pituitary gland to release endogenous opioid peptide substances that reduce pain sensitivity. The aim of the study was to determine Hegu acupoint stimulation effect on the pain sensitivity reduction in maxillary central incisor gingiva. The laboratory experimental research was conducted on 12 healthy male Wistar rats (3 months old, weights 150–200 grams). All rat samples received the same treatments and adapted within 1 month. The research was done in pre and post test control group design. 40-Volt electro-stimulation was done once on the maxillary central incisor gingiva prior to the bilateral Hegu acupoint stimulation, then followed by 3 times electro-stimulation with 3 minutes intervals. The pain scores were obtained based on the samples’ contraction in each electro-stimulation. The responses were categorized into 5 pain scores and statistically analyzed using Wilcoxon Test. The results showed that Hegu acupoint stimulation lowered the pain scores significantly (p < 0.05). Hegu acupoint stimulation could reduce the pain sensitivity in maxillary central incisor gingiva. Therefore, the use of acupuncture analgesia in dental pain management can be considered in the future.]]>
<![CDATA[Ekspresi produk gen laten virus epstein-barr pada karsinoma sel skuamosa rongga mulut (The expressions of latent gene product of epstein-barr virus in oral squamous cell carcinoma) ]]>
<![CDATA[Theresia Indah Budhy S]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 2 (2005): June 2005 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
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DOI: 10.20473/j.djmkg.v38.i2.p84-87
<![CDATA[Squamous cell carcinoma (SCC) is a type of cancer often found in oral cavity and the area of head and neck at about 90%. Based on the geographical incidence oral SCC (OSCC) has many types of different emerging. This case probably has connection with ethnic group, habit and social and economical condition. In East Java, the incidence is about 2.64% and it increases every year. The virus is known as one of the main factors that result in this disease. Epstein-Barr virus (EBV) has potential capability of carcinogenesis. EBV is the family of herpesviridae that can infect cell through the linking of CD 21 receptor of the epithel with glycol protein 350/220 of the virus capsule. After primary infection, the virus will form latent-gene in human cell. Periodically, the latent-gene product can disturb proliferation and apoptotic regulator. In Indonesia, the expression of EBV latent geneproduct in the OSCC has not been reported yet. This study wanted to know the expression of EBV latent gene product found in the OSCC. This study found 25 cases of OSCC in which 17 were infected by EBV. Detection of EBV infection could be done by insitu hybridization to identify RNA EBV (EBER). To find the expression of EBV latent gene product, immunohistochemical analysis was done. The conclusion was that the emerging of expression of EBV latent gene product in OSCC were latent membrane protein-1 (LMP-1), EBV nuclear antigen-1 (EBNA-1) and RNA EBV (EBER). They were 28.28%, 25.26% and 46.47%. It was suggested to do the following research on OSCC infected by EBV and the emerging of expression of EBV latent gene product with regulator gene of proliferation and apoptotic in OSCC.]]>
<![CDATA[The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction ]]>
<![CDATA[Bayu Indra Sukmana]]>;
<![CDATA[Theresia Indah Budhy]]>;
<![CDATA[I Gusti Aju Wahju Ardani]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
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DOI: 10.20473/j.djmkg.v50.i1.p36-42
<![CDATA[Background: The main oral health problem in Indonesia is the high number of tooth decay. Tooth extraction is the treatment often received by patients who experience tooth decay and the wound caused by alveolar bone resorption. Bark of Mangifera casturi has been studied and proven to contain secondary metabolite which has the ability to increase osteoblast’s activity and suppress osteoclast’s activity. Purpose: The purpose of this study was to analyze interleukin-1 beta (IL-1β) and bone morphogenic protein-2 (BMP-2) activities during bone remodeling after Mangifera casturi’s bark extract treatment. Method: This study was laboratory experimental research with randomized post-test only control group design. The Mangifera casturi bark was extracted using 96% ethanol maceration and n-hexane fractionation. This study used 40 male Wistar rats which are divided into 4 groups and the tooth extraction was performed on the rats’ right mandible incisive tooth. The four groups consisted of 6.35%, 12.7%, 25.4% extract treatment group, and a control group. Wistar’s mandibles were decapitated on the 7th and 14th day after extraction. Antibody staining on preparations for the examination of IL-1β and BMP-2 expressions was done using immunohistochemistry. Result: There was a significant difference of IL-1β and BMP-2 expressions in 6,35%, 12,7%, and 25,4% treatment groups compared to control group with p<0.05. Conclusion: Mangifera casturi’s bark extract was able to suppress the IL-1β expression and increase the BMP-2 expression during bone remodeling after tooth extraction.]]>
<![CDATA[The potential effect of Moringa oleifera leaves extract on vascular endothelial growth factor expression in Wistar rat oral cancer cells ]]>
<![CDATA[Dwicha Rahma Nuriska Hartono]]>;
<![CDATA[Theresia Indah Budhy Sulisetyawati]]>;
<![CDATA[Edhi Jularso]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 2 (2019): June 2019 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
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DOI: 10.20473/j.djmkg.v52.i2.p71-75
<![CDATA[Background: Cancer is a disease characterized by abnormal and uncontrolled growth of tissue cells. In Indonesia, cancer ranks as the fifth largest cause of mortality, while it is the second largest worldwide. Cancer affected by angiogenesis, the process of forming new blood vessels to provide the nutrient and oxygen necessary for tumor growth. Vascular endothelial growth factor (VEGF) represents a pro-angiogenic factor. Moringa oleifera leaf extract can be used as an anticancer agent by reducing the expression of VEGF. Purpose: The study aimed to prove that Moringa oleifera leaf extract can reduce VEGF expression in benzopyrene-induced oral cancer cells of Wistar rats. Methods: This was an experimental laboratory research with posttest-only control group design. All experimental subjects presented symptoms of cancer following induction with 8 mg/KgBW of benzopyrene. The sample consisted of 28 Wistar rats, divided into four groups, namely; a control group (K) whose members were administered with only aquadest and three treatment groups (P) treated with Moringa oleifera leaves extract at percentages of 3.125% (P1), 4.6875% (P2) and 6.25% (P3) respectively. Observation of VEGF expression was undertaken by means of immunohistochemical staining. Results: A decrease in VEGF expression occurred in all treatment groups when compared with the control group. A significant difference existed between the control group (K) and the treatment group (P1), while there were no significant differences between the treatment groups (P1, P2 and P3). Conclusion: Moringa oleifera leaf extract with a percentage of 3.125% proved most effective at reducing VEGF expression in oral cancer cells in Wistar rats.]]>
<![CDATA[The potential of ethanolic extract of Moringa oleifera leaves on HSF1 expression in oral cancer induced by benzo[a]pyrene ]]>
<![CDATA[Vania Syahputri]]>;
<![CDATA[Theresia Indah Budhy]]>;
<![CDATA[Bambang Sumaryono]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 2 (2020): June 2020 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
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DOI: 10.20473/j.djmkg.v53.i2.p107-110
<![CDATA[Background: Oral cancer is the sixth most common malignancy that occurs in the world, with more than 330,000 deaths a year. In cancer, mutations occur in proteins, accompanied by unfolding proteins, caused by the unstable micro-environment in cells. To stabilise this condition, protein protectors called heat shock proteins (HSPs) are needed. HSPs are activated by a group of transcription factors known as heat shock factor 1 (HSF1). HSF1 is a considered target in cancer therapy. Moringa oleifera leaves are known to have anti-cancer properties because of bioactive compounds called flavonoid and isothiocyanate and are used as herbal therapy for cancer. Purpose: To investigate the potential effect of ethanolic extract of Moringa oleifera on HSF1 expression in oral cancer induced by benzo[a]pyrene. Methods: This study used 25 male Wistar rats divided into five groups consisting of the negative control group (K-), which was only given aquadest; the positive control group (K+), which was induced with benzo[a]pyrene and given aquadest; and treatment groups that were induced with benzo[a]pyrene and given Moringa oleifera leaf extract at concentrations of 3.125% (P1), 6.25% (P2), and 9.375% (P3). Examination of HSF1 expression was carried out by immunohistochemistry staining. Data were analysed using the Kruskal–Wallis test and post-hoc Tukey HSD. Results: HSF1 expression in the P1, P2, and P3 groups decreased significantly compared to the K+ group. There were no significant differences between the P1, P2, and P3 groups (p > 0.005). Conclusion: Ethanolic extract of Moringa oleifera leaves in three concentrations can decrease expression of HSF1 in oral cancer induced by benzo[a]pyrene.]]>
<![CDATA[Penentuan Grading Tumor Ganas Oral Squamous Cell Carcinoma Berdasarkan Gambaran Histopatologi ]]>
<![CDATA[Theresia Indah Budhy]]>
<![CDATA[ Jurnal Biosains Pascasarjana Vol. 17 No. 1 (2015): JURNAL BIOSAINS PASCASARJANA ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/jbp.v17i1.2015.46-51
<![CDATA[AbstrakLatar Belakang: Sekitar 95% dari tumor ganas diklasifikassikan secara histologis sebagai Oral Squamous Cell Carcinoma (OSCC). Secara mikroskopis OSCC diklasifikasikan berdasarkan pada metode yang mempehitungkan penilaian subjektif, tingkat keratinisasi, pleomorfik nukleus dan seluler, dan aktivitas mitosis. Tingkatan tersebut meliputi well differentiated (grade I-II), moderately differentiated (grade III), dan poorly differentiated (grade IV). Well dan moderately differentiated dapat dikelompokkan sebagai low grade sedangkan poorly differentiated tumor sebagai high grade. Standar yang paling baik untuk menegakkan diagnosis OSCC adalah pemeriksaan histopatologis dan biopsi jaringan lesi tersebut. Tujuan: Penelitian ini bertujuan untuk menentukan grading tumor ganas OSCC berdasarkan gambaran histopatologi. Metode: Sampel terdiri dari 6 jenis OSCC kemudian dilakukan pembuatan sediaan jaringan tumor ganas OSCC kedalam blok paraffin dan dilakukan pengecatan menggunakan hematoxilin eosin (HE). Gambaran histopatologi dari keenam sampel diamati dibawah mikroskop cahaya dengan pembesaran 100x dan 400x. Hasil: Dari 6 kasus yang ditemukan satu terdiagnosa sebagai well differentiated (stage I), dua kasus terdiagnosa well differentiated(sstage II), dua kasus terdiagnosa moderately differentiated (stage III), dan poorly differentiated (stage IV). Kesimpulan: OSCC diklasifikasikan berdasarkan gambaran histopatologi kedalam well differentiated (stage I-II), moderately differentiated (stage III), dan poorly differentiated (stage IV)Kata Kunci : OSCC, well differentiated, moderately differentiated, poorly differentiated]]>
