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All Journal e-GIGI Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Scientific Dental Journal Journal of Indonesian Dental Association Jurnal Material Kedokteran Gigi Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu Sriwijaya Journal of Dentistry
Dewi Liliany Margaretta, Dewi Liliany
Bagian Dental Material, Fakultas Kedokteran Gigi Universitas Trisakti, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Macerated-Pineapple Core Crude Extract-derived Bromelain Has Low Cytotoxic Effect in NIH-3T3 Fibroblast Dewi Liliany Margaretta; Angliana Chouw; Yanni Dirgantara; Melanie Sadono Djamil; Ferry Sandra
The Indonesian Biomedical Journal Vol 7, No 2 (2015)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v7i2.75

Abstract

BACKGROUND: Bromelain is a sulfhydryl proteolytic enzyme that can hydrolyze protein, protease or peptide. Bromelain can be found in pineapple stem, fruit and core. Bromelain is composed of 212 amino acid residues with cysteine-25 forming a polypeptide chain that can hydrolyze peptide bonds by H2O. In medicine, bromelain has been developed as antibiotic, cancer drug, anti-inflammatory agent and immunomodulator. In dentistry, bromelain has potential to reduce plaque formation on the teeth and to irrigate root canal.METHODS: Pineapple core was dried for 3 days to get simplicia. Then simplicia was extracted with water solvent for 24 hours. After that, the macerated-pineapple core crude extract-derived bromelain (PCB) was separated by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue (CBB) staining to ensure the presence of bromelain. In cytotoxic test, NIH-3T3 fibroblast cultures were treated with extracts in various concentrations to for 24 or 48 hours. Number of fibroblasts was calculated using 3-(4,5-dimethylthiazol-2- yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Pineapple core extraction using maceration method produced relative high yield (concentration: 1.5424 g/mL) of bromelain, which was confirmed by CBB staining results with the molecular weight of 33 kDa. Based on cytotoxic test results of PCB on NIH-3T3 fibroblasts, 24-hours-incubation LD50 was 95.7 g/L, while 48-hours-incubation LD50 was 51.1 g/L.CONCLUSION: PCB has low cytotoxic effect in NIH-3T3 fibroblasts.KEYWORDS: bromelain, pineapple, extract, cytotoxic, MTT
Enzymatic Activity of Bromelain Isolated Pineapple (Ananas comosus) Hump and Its Antibacterial Effect on Enterococcus faecalis Dewi Liliany; Armelia Sari Widyarman; Erni Erfan; Janti Sudiono; Melanie Sadono Djamil
Scientific Dental Journal Vol. 2 No. 2 (2018): May
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i2.2540

Abstract

Background: Enterococcus faecalis is the frequent cause of oral infections, such as periodontitis, infected root canals, and peri-radicular abscesses. Pineapple (Ananas comosus) fruit contains bromelain, one of proteolytic enzymes associated with several health benefits. Bromelain has been shown to promote healthy digestion, stimulate the immune system, improve cardiovascular conditions, and accelerate wound healing. Bromelain compounds possess anti-inflammatory and anticancer properties and exhibit antibacterial activity. Objective: To analyze the enzymatic activity of bromelain extracted from pineapple hump and investigate the antibacterial effect of bromelain against E. faecalis. Methods: Pineapple hump was dried and extracted with maceration technique. Further purification was obtained by ammonium sulfate fractionation, dialysis and ion exchange chromatography. Minimum inhibitory concentration (MIC) tests using diffusion and dilution techniques tested the antibacterial activity of the bromelain extract on E. faecalis. A one-way analysis of variance (ANOVA) test analyzed the significance of the differences in the E. faecalis inhibition zones after treatment with a range of bromelain extract concentrations. Differences were considered statistically significant if p < 0.05. Results: The specific activity of bromelain in the crude extract was 62.89 U/mg. Furthermore, bromelain activity using ammonium sulfate fractionation was 50.99 U/mg, dialysis was 54.59 U/mg, and ion exchange chromatography was 152.38 U/mg. The bromelain extract showed effective inhibitory and bactericidal activity against E. faecalis. The results of the inhibition test using a bromelain extract purified by ion exchange  chromatography demonstrated that a concentration as small as 12.5% was effective in inhibiting the growth of E. faecalis (p < 0.05). Conclusion: The highest enzymatic activity of bromelain was found after purification with ion exchange chromatography. Bromelain exerted an antibacterial effect against a potent endodontic pathogen, but further studies are needed to explore this effect.
Perbedaan Pengaruh Pasta Gigi Berbahan Dasar Hidroksiapatit dan Eugena caryophillus Terhadap Hipersensitifas Gigi Risya Aulia; Dewi Liliany Margaretta; Rosalina Tjandrawinata
Jurnal Material Kedokteran Gigi Vol 7 No 1 (2018): JMKG Vol 7 No 1 Maret 2018
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.979 KB) | DOI: 10.32793/jmkg.v7i1.280

Abstract

Tooth hypersensitivity is a clinical condition that describes localized, non spontaneous pain, and arises when tooth is stimulated using thermal, chemical, and mechanical stimuli. The use of desensitizing agent such as hydroxyapatite and Eugenia caryophyllus can be used as home treatment that can relieve tooth hypersensitivity. Hydroxyapatite is an inorganic compound that makes up the hard tissues of the human body such as bone, dentine and tooth. Hydroxyapatite has a mechanism to decrease dental hypersensitivity by inducing remineralization of the teeth and close the open dentinal tubules. Eugenia caryophyllus is a material that is used in the field of dentistry and can reduce the pain of tooth hypersensitivity by suppressing nerve receptors in the perception of pain felt by the patient. The aim of this study is to determine the influence of hydroxyapatite-based toothpaste and Eugenia caryophyllus against tooth hypersensitivity. In this observational research, researcher give different type of toothpaste to two groups of research subjects and measure the changes in tooth sensitivity for 15 days. The value of tooth hypersensitivity before and after treatment is tested using Electric Pulp Test (EPT). Independent t-test shows the result of p value = 1.30 (p>0.05). It can be concluded that there is no significant difference between hydroxyapatite and Eugenia caryophyllus in relieving tooth hypersensitivity after being used for 15 days.
Pengaruh Obat Kumur Mengandung Alkohol 9% Dan Non-Alkohol Terhadap Kekuatan Tekan Resin Komposit Bulk-Fill (Penelitian) Gabriella Sun; Dewi Liliany Margaretta
Jurnal Kedokteran Gigi Terpadu Vol. 1 No. 2 (2019): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (751.824 KB) | DOI: 10.25105/jkgt.v1i2.6339

Abstract

Background: Bulk-fill composite resin is a tooth-colored restoration material that is developed to obtain better aesthetic and mechanical properties, which include compressive strength. The factors influencing the reduction of compressive strength of composite resin are hydrophilic matrix monomers and inadequate bonds between compositions. One of the absorbable liquids is a mouthwash commonly used to control dental caries and reduce the amount of plaque after brushing the teeth. Objective: To determine the effect of mouthwash containing 9% alcohol and nonalcohol on the compressive strength of bulk-fill composite resin. Method: The research type conducted is an experimental laboratory. The compressive strength of bulk-fill composite resin will be tested using Universal Testing Machine. There were three treatment groups, which are immersion in mouthwash containing 9% alcohol, nonalcohol, and artificial saliva as a control group. Samples were immersed at room temperature 25°C for 12 hours according to instructions for use twice a day for 30 seconds. Results: The One Way ANOVA test shows a significant value, which was sig. 0.001 (p < 0.05). There were significant differences in the test of the effect of mouthwash containing 9% alcohol and nonalcohol mouthwash on the compressive strength of bulk-fill composite resin. This result is caused by the hydrophilic traits of the matrix monomers, mechanisms that occur in resin compositions, acidity and alcohol content of mouthwash can affect the reduction in compressive strength of the composite resin. Conclusion: The mouthwash containing 9% alcohol reduced the compressive strength of bulk-fill composite resin more compared to nonalcohol mouthwash. 
Evaluasi Perubahan Warna Semen Ionomer Kaca dengan Penambahan Ekstrak Etanol Propolis Trigona spp. Deviyanti Pratiwi; Advita Azalia; Achmad E. Z. Hasan; Florencia L. Kurniawan; Dewi L. Margaretta
e-GiGi Vol. 11 No. 2 (2023): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v11i2.46928

Abstract

Glass ionomer cement (GIC) is constantly modified to improve its properties and expand its use in dental practice. One of the modifications studied is the addition of ethanolic extract of propolis (EEP), a natural resin material with various pharmacological effects. Modifications made with EEP were found to increase the antibacterial effect of GIC, but this modified material also impacted the physical properties of the cement. This study aimed to evaluate the color change of GIC caused by the addition of EEP. This was a laboratory experimental study. The GIC color was assessed using the VITA Easyshade V spectrophotometer. A total of 20 cylindrical GIC samples measuring 10 mm (diameter) x 2 mm (height) were divided into four groups based on the proportion of EEP added to the GIC liquid, as follows: A, conventional GIC (control); B, GIC modified with EEP at 25% w/w; C, GIC modified with EEP at 30% w/w; and D, GIC modified with EEP at 35% w/w. The color assessment was performed after the samples were immersed in artificial saliva and incubated for 24 hours. The post hoc test between the three experimental groups and the control group showed a significant difference (p<0.05). The ΔE value between the three experimental groups and the control group showed a value greater than 3.3. In conclusion, the addition of EEP in the proportions of 25%, 30%, and 35% resulted in significant color change of GIC. Keywords: glass ionomer cement; ethanolic extract of propolis; Trigona spp.; discoloration   Abstrak: Semen ionomer kaca (SIK) terus dimodifikasi untuk meningkatkan sifat dan memperluas penggunaannya dalam praktik kedokteran gigi; salah satunya ialah penambahan ekstrak etanol propolis (EEP), suatu bahan resin alami yang memiliki berbagai efek farmakologis. Modifikasi dengan EEP dapat meningkatkan efek antibakteri dari SIK, namun bahan modifikasi ini juga berdampak terhadap sifat fisik semen. Penelitian ini bertujuan untuk mengevaluasi perubahan warna SIK yang disebabkan oleh penambahan EEP Trigona spp. Jenis penelitian ialah eksperimental laboratorik. Warna diukur menggunakan spektrofotometer VITA Easyshade V. Sebanyak 20 sampel SIK silinder berukuran 10 mm (diameter) x 2 mm (tinggi) dibagi dalam empat kelompok uji berdasarkan proporsi EEP yang ditambahkan ke cairan SIK: A, SIK konvensional (kontrol); B, SIK modifikasi EEP 25% w/w; C, SIK modifikasi EEP 30% w/w; dan D, SIK modifikasi EEP 35% w/w. Pengukuran warna dilakukan setelah sampel direndam dalam saliva buatan dan diinkubasi selama 24 jam. Uji post hoc antara ketiga kelompok perlakuan dengan kelompok kontrol menunjukkan perbedaan bermakna (p<0,05). Nilai ΔE antara ketiga kelompok perlakuan dengan kelompok kontrol menunjukkan nilai yang lebih besar dibandingkan 3,3. Simpulan penelitian ini ialah penambahan EEP dalam proporsi 25%, 30%, dan 35% secara bermakna mengakibatkan perubahan warna SIK. Kata kunci: semen ionomer kaca; ekstrak etanol propolis; Trigona spp.; perubahan warna
Pendidikan dan Pelatihan Pemilahan Sampah Domestik untuk Meningkatkan Kesehatan Tubuh dan Rongga Mulut Warga RW 01 Kelurahan Grogol Petamburan Dewi Liliany Margaretta; Rosalina Tjandrawinata; Dina Ratnasari; Harris Gadih Pratomo; Yessy Ariesanti
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 2 No. 1 (2023): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v2i1.16797

Abstract

The problem of domestic waste in Indonesia related to the behavior of people who do not understand the impact of poor environmental hygiene which will lead to the emergence of various diseases in the body and oral cavity. Domestic waste is the remaining waste generated from household activities. Domestic waste is divided into organic that can be processed and inorganic that cannot be processed. The most pronounced impact is the emergence of various diseases such as intestinal worms, diarrhea, cholera, typhoid and anemia while infection can occur in the oral cavity so that infection can occur locally or systemically. Community Service (PkM) was carried out offline at the Grogol Petamburan Hall which was attended by 37 representatives of dasawisma and jumantik cadres, heads of RT 01 – 14 and RW 01. The material provided was in the form of various kinds of domestic waste (organic and inorganic), management of domestic waste, diseases that arise in the body and oral cavity due to a dirty environment, how to wash hands and how to brush your teeth properly in the form of ppt, e-flyer and video. Evaluation of success by conducting a pre and post test containing 10 knowledge questions and 10 attitude questions. There was an increase in participants knowledge of 22.3% and an improvement in attitude towards a better one. The results obtained from this activity are very good with partners willing to carry out PkM activities. At the end of the activity, toothpaste and a donation of trash bins were given to each RT.
Pengaruh suhu air terhadap setting time dari bahan cetak alginat Tansza S. Putri; Deviyanti Pratiwi; Eddy; Rosalina Tjandrawinata; Dewi L. Margaretta; Florencia L. Kurniawan; Octarina
e-GiGi Vol. 12 No. 1 (2024): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v12i1.47105

Abstract

Abstract: Irreversible hydrocolloid impression materials or alginate is commonly used in dental practice. Its setting time range is 1–4.5 minutes. The setting time is affected by several factors such as water temperature mixed with the alginate powder. There are previous studies evaluated the effect of water temperature on the alginate’s setting time, however, the collected data is still minimal. Therefore, this study aimed to evaluate the effect of water temperature on alginate’s setting time on larger scale. There were total of 423 samples divided into three groups: cold, moderate, and warm temperature of water mixed with alginate; each group consisted of 141 samples. Differences in setting times of the qroups were analyzed with the Kruskal-Wallis test and the Tukey’s HSD. Alginate powder used in this study was the normal-set type. Mixing the alginate powder with water was performed as the factory instruction, and then the mixture was poured into molds and the setting times were measured. The results showed that the setting times of the three groups were significantly different. Group I (cold) had the highest setting time (211 seconds), followed by Group II (room temperature) which was 147 seconds, and Group III (warm) had a setting time of 106 seconds.  In conclusion, water temperature has an effect on setting time, that is, the higher the temperature, the faster the setting time, and vice versa. Keywords: alginate; irreversible hydrocolloid; setting time; water temperature   Abstrak: Bahan cetak hidrokoloid ireversibel atau alginat merupakan bahan umum yang digunakan di praktek kedokteran gigi, dan memiliki setting time antara 1–4,5 menit. Setting time ditentukan oleh beberapa faktor di antaranya suhu air yang digunakan dalam mencampur bahan cetak tersebut. Terdapat penelitian-penelitian terdahulu yang mengevaluasi efek suhu terhadap setting time, namun data yang dikumpulkan masih minimal. Penelitian ini bertujuan untuk mengetahui efek suhu terhadap setting time bahan cetak alginat dalam skala besar. Terdapat tiga kelompok penelitian yaitu menggunakan suhu air yang dicampurkan dengan alginat dingin, sedang (suhu ruang), dan hangat. Besar sampel penelitian ini ialah 141 sampel per kelompok, sehingga total sampel ialah 423 sampel. Perbandingan setting time dari tiga kelompok kemudian dianalisis secara statistik menggunakan uji Kruskal-Wallis dan Tukey’s HSD untuk mengetahui ada tidaknya perbedaan bermakna dari kelompok-kelompok tersebut. Bubuk alginat yang digunakan merupakan alginat tipe normal. Pencampuran bubuk alginat dengan air dilakukan sesuai dengan instruksi pabrik. Adonan yang telah diaduk dituang ke dalam cetakan dan dilakukan pengukuran setting time. Hasil penelitian mendapatkan setting time dari ketiga kelompok berbeda secara bermakna. Kelompok I (dingin) memiliki setting time tertinggi yaitu selama 211 detik, diikuti kelompok II (sedang) selama 147 detik, dan kelompok III (hangat) selama 106 detik. Simpulan penelitian ini ialah suhu air memiliki efek terhadap setting time, yaitu semakin tinggi suhu air maka semakin singkat setting time, demikian pula sebaliknya. Kata kunci: alginat; hidrokoloid ireversibel; setting time; suhu air
Degradation of chitosan–gelatin and chitosan–gelatin–β-tricalcium phosphate scaffolds Putri, Tansza Setiana; Pratiwi, Deviyanti; Margaretta, Dewi Liliany; Tjandrawinata, Rosalina; Shariff, Khairul Anuar
Dental Journal (Majalah Kedokteran Gigi) Vol. 57 No. 2 (2024): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i2.p87-90

Abstract

Background: Fabrication of the composite scaffold was carried out by combining chitosan, gelatin, and β-tricalcium phosphate (βTCP) derived from limestone. The extraction of βTCP was based on the abundance of limestone containing calcium carbonate, which can be a source of βTCP synthesis. Purpose: This study evaluates the degradation of the combination of chitosan–gelatin (ChG) and chitosan–gelatin–βTCP (ChG-βTCP) composite scaffolds. Methods: The freeze-drying method was used to obtain the composite scaffold, which was a mixture of chitosan, gelatin, and βTCP. Degradation was measured by immersing the samples in a simulated body fluid solution at 37°C for 3, 7, 14, and 21 days. For statistical analysis, one-way analysis of variance (ANOVA) and post hoc Fisher's least significant difference were performed. Results: The ChG scaffold shows better degradability than the ChG-βTCP scaffold. The ChG scaffold shows higher weight degradation than the ChG-βTCP scaffold up to 21 days. Conclusion: In conclusion, the scaffold containing βTCP has lower degradation than the ChG scaffold.
Effect of Red Betel Leaf Infusion (Piper crocatum) on Physical and Mechanical Properties of Nanohybrid Composite Resins Wulan Dhari, Maria Rini; Margaretta, Dewi Liliany; Tjandrawinata, Rosalina
Journal of Indonesian Dental Association Vol 7 No 2 (2024): October
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v7i2.1147

Abstract

Introduction – Pendahuluan: Nanohybrid resin composite as a dental restoration material has physical properties (water absorption and color stability) and mechanical properties (hardness). Red betel infusion as a mouthwash, contains tannins and color pigment that will lead to discoloration when accumulated within the resin matrix. Water absorption is one of the supporting factors for color change. The weak acids in red betel lead result breakdown of resin matrix bonds and a decrease in hardness Objective – Tujuan: To analyze the effect on hardness and color change of nanohybrid resin composite after immersion in red betel infusion Methods – Metode: Samples of nanohybrid composite resin were shaped cylindrical with a diameter of 10 mm and a height of 2 mm. The samples were formed using a stainless steel mold. The treatment group was immersed in red betel infusion for 30 minutes with rinsing intervals, followed by immersion in artificial saliva for 23 hours and 30 minutes for 12 and 15 days. Samples in control group were immersed in artificial saliva for 24 hours. The water absorption was measured using analytical balance, the color change was measured using the VITA Easyshade V, and hardness using a Vickers Microhardness Tester. Results – Hasil: One Way ANOVA results for the water absorption (p= 0.268), ΔE (overall color change) (p= 0.000), ΔL (p= 0.444), ΔC (p= 0.001), ΔH (p= 0.012), and hardness (p= 0.000). Post Hoc Tukey results, significant differences in ΔE (overall color change), ΔC, and hardness between days 12 and 15 compared to the control, and ΔH on day 15 compared to the control Conclusion – Kesimpulan: The hardness of the nanohybrid resin composite decreases, and overall color change occurs after immersion in red betel leaf for 12 and 15 days
Uji Waktu Pengerasan Gipsum sebagai eksperimen Skills Lab di Universitas Trisakti Margaretta, Dewi Liliany; Eddy, Eddy; Pratiwi, Deviyanti; tjandrawinata, rosalina; Octarina, Octarina; Kurniawan, Florencia Livia; Putri, Tansza Setiana
Jurnal Material Kedokteran Gigi Vol 11 No 1 (2022): JMKG Vol 11 No 1 Maret 2022
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v11i1.1033

Abstract

Gypsum is a material that is commonly used in dentistry as study model or working model. The accuracy of the working model is influenced by the absence of defects in the model. Dental students need to know the gypsum setting time in order to avoid fracture of the model when opening the model from negative impression before reaching final setting time. Gypsum setting time can be affected by several factors such as water temperature, water ratio, room temperature and mixing speed. Measurement of gypsum setting time has been done before, however, the number of samples used was still few. Therefore, the aim of this study was to determine the setting time of gypsum with different water/powder ratio on a big number of samples. This research was done by dental students of Faculty of Dentistry, Universitas Trisakti as one of Dental Material’s Courses. A total of three types of water ratios were used in this study, namely 27 cc, 30 cc and 33 cc (n = 141). Type III gypsum powder was weighed with the same weight of 100 gr and then mixed by different water ratios for 60 seconds. Gypsum mixture was placed on a container and vibrated for 60 seconds to eliminate pores. The placement of a small needle to measure the setting time begins at the 150th second and was repeated in multiples of 30 seconds until the tip of the needle did not penetrate the gypsum mixture. The results showed that more water ratio caused the setting time of gypsum type III increased.
Co-Authors Achmad E. Z. Hasan Adis, Zaldy Farhan Advita Azalia Angliana Chouw Ariesanti, Yessy Caroline Caroline Deviyanti Pratiwi Dina Ratnasari Eddy Eddy Eddy Eddy, Eddy Erni Erfan, Erni Ferry Sandra Florencia Livia Kurniawan Gabriella Sun Harris Gadih Pratomo Iskandar, Gavin Benedict Janti Sudiono Kamadi, Jason Kusnadi, Stephanie Justine Melanie Sadono Djamil Melanie Sadono Djamil Octarina Octarina Octarina Risya Aulia Rosalina Tjandrawinata Shariff, Khairul Anuar Subrata, Aryadi Tansza Setiana Putri Violetta, Vanya Widyarman, Armelia Sari Wulan Dhari, Maria Rini Yanni Dirgantara