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All Journal Jurnal Kesehatan Poltekkes Kemenkes RI Pangkalpinang JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Variable Research Journal Journal of Biomedical Sciences and Health
Pribadhi, Adhitya Naufal
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Identifikasi Bakteri Eschericia Coli pada Sumber Air Minum yang Ada di Kantin Sekolah Dasar Pamungkas, Aditya Aji; Pribadhi, Adhitya Naufal; Suwanto, Yustiana Arie
JURNAL KESEHATAN POLTEKKES KEMENKES RI PANGKALPINANG Vol 11, No 2 (2023): JKP Desember 2023
Publisher : Poltekkes Kemenkes Pangkalpinang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32922/jkp.v11i2.866

Abstract

Latar belakang: Anak sekolah sangat menyukai konsumsi jajanan minuman yang ada di kantin sekolah. Minuman ini berkemungkinan terkontaminasi oleh bakteri terutama bakteri Coliform. Peraturan Menteri Kesehatan Republik Indonesia Nomor 492/Menkes/Per/IV/2010 menyatakan air minum dinyatakan aman bagi kesehatan apabila memenuhi persyaratan fisika, mikrobiologis, kimiawi dan radioaktif. Pada persyaratan mikrobiologis jumlah Coliform dan Eschericia coli dalam  air  tidak boleh melewati batas yang ditentukan yaitu 0/100 ml sampel air.Tujuan: Untuk mengetahui hubungan sumber air minum dengan kandungan bakteri Eschericia coli dalam air minum di kantin sekolah dasar Kecamatan Tugu SemarangMetode: Metode yang digunakan dalam penelitian ini adalah observasional analitik dengan rancangan cross sectional menggunakan data primer dimana sampel diambil langsung dari seluruh kantin sekolah dasar Kecamatan Tugu SemarangHasil: Total 14 sampel air minum yang didapatkan dari seluruh kantin sekolah dasar Kecamatan Tugu Semarang hanya ada dua jenis sumber air minum yang digunakan, yaitu air sumur dan air minum isi ulang. Ditemukan tiga sampel positif bakteri escherichia coli dari air minum isi ulang. Pada uji statistik Chi square dengan uji alternatif Fisher’s Exact didapatkan hasil p 0,604 > 0,05.Kesimpulan: Tidak terdapat hubungan antara sumber air minum dengan kandungan bakteri Eschericia coli dalam air minum di kantin sekolah dasar Kecamatan Tugu Semarang. 
In vitro antimicrobial activity of selected herbal extracts against Mycobacterium tuberculosis Shahab, Fatinah; Pribadhi, Adhitya Naufal
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 16, No 1, (2025)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol16.Iss1.art3

Abstract

Background: Tuberculosis (TB) is one of the deadliest infectious diseases worldwide, causing approximately two million deaths annually. Multidrug resistance presents a significant global challenge in TB treatment, necessitating prolonged medication use and extended treatment durations. There is an urgent need to discover alternative novel antimicrobial compounds. Herbal medicine offers promising prospects for drug discovery and development, warranting the screening of plant-derived compounds with favorable safety margins. Objectives: This study aimed to investigate the in vitro antimicrobial activity of extracts from five medicinal plants against Mycobacterium tuberculosis in vitro.Methods: Extracts from five herbs —Morinda citrifolia L, Nicotiana spp.L, Centella asiatica L, Allium sativum L, and Annona muricata L, —were investigated for their in vitro antimicrobial activity against M. tuberculosis using Lowenstein-Jensen medium. Antimicrobial activity was assessed by comparing bacterial colony growth on extract-containing media with that on extract-free control media. The agar dilution method was used to determine antimicrobial activity.Results: Among the tested extracts, Nicotiana spp L and Annona muricata L exhibited the strongest antimicrobial activity. These herbs contain various bioactive compounds, including alkaloids, flavonoids, and steroids, which are presumed to contribute to bacterial susceptibility. Conclusions: The findings suggest that Nicotiana spp. L. and Annona muricata L. may have therapeutic potential in TB treatment. These herbs could serve as alternative agents for combating TB and may help mitigate the adverse effects associated with standard anti-tuberculosis drugs.
The Bacteriocin Potential of Lactic Acid Bacteria from Cincalok as an Antibacterial Against Shigella and Salmonella Aprilia, Ineke; Pribadhi, Adhitya Naufal; Suwanto, Yustiana Arie; Widyaningrum, Ratna; Yana, Dian Rudy; Rofiah, Nurul Ummi
Journal of Biomedical Sciences and Health Vol. 3 No. 1 (2026)
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/jbsh.v3.i1.304

Abstract

Background: Lactic Acid Bacteria are a group of bacteria that have the ability to produce antimicrobial compounds called bacteriocins. Antibacterial of lactic acid bacteria proved effective in inhibiting pathogenic bacteria that cause infection in humans such as Shigella sp and Salmonella sp. Lactic acid bacteria can be found in fermented foods, one of which is fermented food typical of West Kalimantan, namely cincalok Aim: This study was to determine the antibacterial potential of cincalok lactic acid bacteria against Shigella sp and Salmonella sp Methods: This study used a true experimental, with the research design using a post test only control group design with data analyzed using SPSS to evaluate inhibition zone differences among three treatment groups: bacteriocin from Weissella sp. at 24 hours, Weissella sp. at 48 hours, and antibiotics (positive control) Results: The results obtained in the bacteriocin test from Weissella showed that there were differences in the inhibition zones formed, in Shigella sp the average values of the inhibition zones formed were W24 (24.4 mm), W48 (13.2 mm) and Cotrimoxazole (42.2 mm). while in Salmonella sp the average value of the inhibition zone formed is W24 (24.2 mm), W48 (12.8 mm) and Chloramphenicol (45 mm). Conclusion: Bacteriocins from LAB cincalok have an antibacterial effect on the growth of Shigella sp and Salmonella sp bacteria.
POTENSI AKTIVITAS ANTIBAKTERI BAKTERI ASAM LAKTAT TC3 YANG DIISOLASI DARI TAUCO FERMENTASI TERHADAP CUTIBACTERIUM ACNES DAN STAPHYLOCOCCUS AUREUS Ramadhan, Bey Jalasukma; Pribadhi, Adhitya Naufal; Sukowati, Eko Gunawan; Suwanto, Yustiana Arie
VARIABLE RESEARCH JOURNAL Vol. 3 No. 01 (2026): JANUARI 2026
Publisher : Media Inovasi Pendidikan dan Publikasi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infeksi kulit dan peradangan jerawat umumnya dikaitkan dengan Staphylococcus aureus dan Cutibacterium acnes, sementara peningkatan penggunaan antibiotik berkontribusi pada resistensi antimikroba dan gangguan mikrobioma. Studi ini menghasilkan aktivitas antibakteri isolat bakteri asam laktat (LAB) TC3 yang berasal dari tauco fermentasi terhadap C. acnes dan S. aureus  menggunakan metode difusi cakram agar. Kultur LAB diinkubasi selama 24 dan 48 jam, dan zona inhibisi diukur dalam milimeter. Klindamisin dan air suling steril digunakan sebagai kontrol positif dan negatif. LAB TC3 yang diinkubasi selama 24 jam menghasilkan zona inhibisi rata-rata 10 ,25 mm terhadap kedua patogen, menunjukkan aktivitas antibakteri sedang. Inkubasi 48 jam menunjukkan inhibisi yang berkurang (9 ,00 mm untuk C. acnes dan 8,50 mm untuk S. aureus ). Klindamisin menunjukkan aktivitas yang lebih tinggi (16 ,13 mm), sedangkan kontrol negatif tidak menunjukkan inhibisi. Hasil ini menunjukkan bahwa LAB TC3 menunjukkan efek antibakteri terukur yang dipengaruhi oleh durasi inkubasi. Meskipun kurang ampuh dibandingkan antibiotik konvensional, isolat ini menunjukkan potensi sebagai kandidat antimikroba tambahan yang kompatibel dengan mikrobioma untuk aplikasi dermatologis
Co-Authors Aprilia, Ineke Fatinah Shahab Pamungkas, Aditya Aji Ramadhan, Bey Jalasukma Ratna Widyaningrum Rofiah, Nurul Ummi Sukowati, Eko Gunawan Suwanto, Yustiana Arie Yana, Dian Rudy