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All Journal HAYATI Journal of Biosciences Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology)
Teja Kisnanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics

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Correlation Between Akt and p53 Protein Expression and Chemoradiotherapy Response in Cervical Cancer Patients IIN KURNIA; BUDININGSIH SIREGAR; SETIAWAN SOETOPO; IRWAN RAMLI; TJAHYA KURJANA; . ANDRIONO; MARINGAN DIAPARI LUMBAN TOBING; BETHY SURYAWATHI; TEJA KISNANTO; DEVITA TETRIANA
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1416.842 KB) | DOI: 10.4308/hjb.21.4.173

Abstract

Akt is a protein that is associated with cell proliferation and is expressed at high levels in cancer cells. Some research indicates it may play a role in increasing the resistance of cancer cells to chemotherapy treatment. P53 is a tumor suppressor protein that influences the cell cycle and apoptosis. The purpose of this study was to examine the relationship between the expression of Akt and p53 in cancerous tissue before chemoradiation treatment, and the clinical response to treatment of cervical cancer patients. Twenty microscopic tissue samples were taken from cervical cancer biopsies obtained from patients before cancer treatment. The tissue samples were stained with p53 and Akt antibodies via immunohistochemistry technique, to measure expression of both proteins. After completion of chemoradiotherapy, patients’ clinical response to treatment was determined using the pelvic control method. Our results revealed no correlation between expression of Akt and p53 index (P = 0.74) as well as between p53 Index and chemoradiotherapy clinical response (P=0.29). There was significant correlation between expression of Akt and cervical cancer chemoradiotherapy response (P = 0.03). There was no correlation found between p53 index and chemoradiotherapy clinical response (P = 0.29). High expression of Akt may related with high cell proliferation and resistance to chemoradiotherapy.
Morfologi dan Tingkat Ekspresi Molekuler Mamosfir Asal Sel Kelenjar Susu Macaca fascicularis yang Diiradiasi Sinar Gamma Sebagai Model Onkogenesis Iin Indriawati; Fitriya Nur Annisa Dewi; Silmi Mariya; Iin Kurnia Hasan Basri; Teja Kisnanto; Dwi Ramadhani; Irma Herawati Suparto
Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) Vol 22, No 1 (2021): February 2021
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jstni.2021.22.1.6141

Abstract

Sel punca memiliki peran penting dalam perkembangan jaringan normal maupun onkogenesis, termasuk pada kejadian kanker payudara. Keterkaitan sel punca dan pembentukan kanker payudara perlu diteliti agar dapat ditemukan metode pencegahan dan terapi baru yang efektif. Teknik kultur mamosfir telah terbukti memperkaya populasi sel punca pada sel kelenjar susu manusia dan satwa primata. Penelitian ini bertujuan untuk mengembangkan model onkogenesis kelenjar susu secara in-vitro dengan induksi sinar gamma pada kultur mamosfir yang berasal dari kelenjar susu monyet ekor panjang (Macaca fascicularis). Analisis morfologi sfir dan uji imunofluoresensi untuk γH2AX dilakukan setelah pajanan iradiasi 4 Gy selama 62 detik pada kultur mamosfir. Penanda molekuler sel punca dewasa dan sel punca kanker dievaluasi menggunakan qRT-PCR. Hasil penelitian menunjukkan bahwa iradiasi sinar gamma menimbulkan kerusakan DNA (double strand break) di dalam sel-sel penyusun mamosfir. Pajanan radiasi juga mengubah morfologi sfir. Ekspresi penanda untuk sel punca dewasa kelenjar susu (CD49f atau Integrin Alpha 6; ITGA6) dan sel punca kanker (CD133 atau Prominin-1; PROM1 dan CD44) lebih tinggi pasca iradiasi dibandingkan dengan kontrol. Temuan ini menunjukkan bahwa kultur mamosfir iradiasi yang berasal dari kelenjar susu monyet berpotensi dikembangkan sebagai model in-vitro untuk studi onkogenesis, terutama yang berkaitan dengan deregulasi sel punca dan risiko kanker payudara. 
Co-Authors . ANDRIONO BETHY SURYAWATHI Budiningsih Siregar Devita Tetriana Dewi, Fitriya Nur Annisa Dwi Ramadhani Iin Indriawati IIN KURNIA Iin Kurnia Hasan Basri Irma Herawati Suparto Ketut Tirtayasa MARINGAN DIAPARI LUMBAN TOBING SETIAWAN SOETOPO Silmi Mariya TJAHYA KURJANA