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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) Microbiology Indonesia Jurnal Ilmu dan Teknologi Kayu Tropis Journal of Stem Cell Research and Tissue Engineering Sanus Medical Journal
Silmi Mariya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Veterinary

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Morfologi dan Tingkat Ekspresi Molekuler Mamosfir Asal Sel Kelenjar Susu Macaca fascicularis yang Diiradiasi Sinar Gamma Sebagai Model Onkogenesis Iin Indriawati; Fitriya Nur Annisa Dewi; Silmi Mariya; Iin Kurnia Hasan Basri; Teja Kisnanto; Dwi Ramadhani; Irma Herawati Suparto
Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) Vol 22, No 1 (2021): February 2021
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jstni.2021.22.1.6141

Abstract

Sel punca memiliki peran penting dalam perkembangan jaringan normal maupun onkogenesis, termasuk pada kejadian kanker payudara. Keterkaitan sel punca dan pembentukan kanker payudara perlu diteliti agar dapat ditemukan metode pencegahan dan terapi baru yang efektif. Teknik kultur mamosfir telah terbukti memperkaya populasi sel punca pada sel kelenjar susu manusia dan satwa primata. Penelitian ini bertujuan untuk mengembangkan model onkogenesis kelenjar susu secara in-vitro dengan induksi sinar gamma pada kultur mamosfir yang berasal dari kelenjar susu monyet ekor panjang (Macaca fascicularis). Analisis morfologi sfir dan uji imunofluoresensi untuk γH2AX dilakukan setelah pajanan iradiasi 4 Gy selama 62 detik pada kultur mamosfir. Penanda molekuler sel punca dewasa dan sel punca kanker dievaluasi menggunakan qRT-PCR. Hasil penelitian menunjukkan bahwa iradiasi sinar gamma menimbulkan kerusakan DNA (double strand break) di dalam sel-sel penyusun mamosfir. Pajanan radiasi juga mengubah morfologi sfir. Ekspresi penanda untuk sel punca dewasa kelenjar susu (CD49f atau Integrin Alpha 6; ITGA6) dan sel punca kanker (CD133 atau Prominin-1; PROM1 dan CD44) lebih tinggi pasca iradiasi dibandingkan dengan kontrol. Temuan ini menunjukkan bahwa kultur mamosfir iradiasi yang berasal dari kelenjar susu monyet berpotensi dikembangkan sebagai model in-vitro untuk studi onkogenesis, terutama yang berkaitan dengan deregulasi sel punca dan risiko kanker payudara. 
Antiproliferative Activities of Anthocephalus cadamba Extracts on Human Breast Cancer and Cervical Adenocarcinoma Cell Lines Rita K Sari; Devi Armilasari; Deded S Nawawi; Wayan Darmawan; Silmi Mariya
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 1 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.95 KB) | DOI: 10.51850/jitkt.v12i1.87

Abstract

The aim of this research was to evaluate the anticancer properties of methanolic extracts from inner bark and wood of jabon (Anthocephalus cadamba). The extracts were investigated in vitro bioassay for its possible antiproliferative activities on human MCF7 breast cancer cell line and HeLa cervical adenocarcinoma cell lines. The cell viability were assessed using microculture tetrazolium technique (MTT) colorimetric assay. The results showed that inner bark extract exhibited higher antiproliferative activity on MCF7 cancer cell line (IC50 91 μg ml-1) than wood extract (IC50 312 μg ml-1). But, antiproliferative activity of inner bark extract on HeLa cell lines was higher (IC50 5 μg ml-1). The inner bark extract is potential to be developed as anti cancer agent in cervical adenocarcinoma cancer therapy because moresecure against Vero normal cells (IC50 288 μg ml-1). Whereas compounds such as phenolic and fatty acid contribute to high antiproliferative activities of inner bark extract. The qualitative analysis detect the extracts containing flavonoids, triterpenoids, saphonin which are thought to contribute to the high antiproliferative activities of this extract.Keywords : Anthocephalus cadamba, antiproliferative activity, human MCF7 breast cancer cell line, HeLa cervical adenocarcinoma cell lines, Vero normal cell lines
3D Differentiation Of Mammosphere Derived Macaca fascicularis’s Mammary Stem Cells silmi mariya
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.104 KB) | DOI: 10.20473/jscrte.v3i1.16330

Abstract

The mammary gland contains adult stem cells that are capable of self-renewal.  This population plays an important role in the development of mammary gland and breast cancer pathogenesis. The studies of mammary stem cells are limited due to the difficulty to acquire and expand adult stem cell population in an undifferentiated state. In this study, we developed mammosphere cultures of nulliparous cynomolgus monkeys (Macaca fascicularis; Mf) as a culture system to enrich mammary stem cells. This species has similarity of mammary gland structure as humans including anatomy, developmental stages, and lobule profile of mammary gland. The use of stem cells from primate animals is essential to bridge the knowledge gaps resulting from stem cell research using rodents for clinical trials in human. Small samples of mammary tissues were collected by surgical biopsy; cells were cultured as monolayer and cryopreserved. Cryopreserved cells were cultured into mammospheres, and the expression of markers for mammary stem cells was evaluated using qPCR. Cells were further differentiated with 3D approaches to evaluate morphology and organoid budding. The study showed that mammosphere culture resulted in an increase in the expression of mammary stem cell markers with each passage. The 3D differentiation in matrigel allowed for organoid formation. Mammary gland stem cells have been successfully differentiated which characterized by CSN2 marker expression and differentiation regulators marker STAT5 and GATA3. The results indicate that mammospheres can be successfully developed derived from breast tissue of nulliparous Mf collected via surgical biopsy. As the mammosphere allows for enrichment of mammary stem cell population, the findings also suggest that a 3-dimensional system is efficient as in-vitro model to study mammary stem cells and a useful system to study mammary differentiation in regards to cancer prevention.
Primary Tupaia javanica Hepatocytes Cultures As In Vitro Replication System for Ape Hepatitis B Viruses MARYATI SURYA; DIAH ISKANDRIATI; SILMI MARIYA; UUS SAEPULOH; PERMANAWATI PERMANAWATI; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.562 KB) | DOI: 10.5454/mi.10.2.3

Abstract

Hepatitis B virus (HBV) is a DNA virus with liver as primary target organ.This virus caused chronic infection that can progress to cirrhosis, liver cancer and even death. In vitro model system of hepatocyte cultures is important and widely used to study a variety aspects of hepatitis B. Development of small animal Tupaia sp. for the in vitro model system is an alternative to the existinghepatocyte cultures. The specific purpose of the studyis to develop Tupaia javanica hepatocytes culture for HBV replication, and in a broader spectrum to answer the need for in vitro model of hepatocytes. Primary T. javanica hepatocytes (PTH) culture was successfully maintained for 14 days to reach 80% confluence, and infection of Javan gibbon HBV (GiHBV) and orangutan HBV (OuHBV) onto the culture on day 15 showed viral replication for up to eight days as measured by polymerase chain reaction (PCR). PCR quantification indicated that the highest copy number of DNA virus was detected onday two anddecreased until day 8 after infection. Cell receptor for HBV attachment, known as sodium taurocholate cotransporting polypeptide was expressed on the surface of PTH and shown as green luminenscent when observed by immunofluorescence assay. Sequence of partialS gene from the apes HBVs after the viruses have been infected to the PTH showed amino acid identity to their wildtype as high as 99.29% for GiHBV and 95.71% for OuHBV. This study suggested that the PTH can support the replication of GiHBV and  OuHBV. 
Peran Sel Punca dalam Pengobatan Regeneratif Silmi Mariya
Sanus Medical Journal Vol. 1 No. 1 (2020)
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/sanus.v1i1.5353

Abstract

Penggunaan sel punca merupakan hal yang sangat menguntungkan bagi ilmu pengetahuan, bagaimana organisme dapat berkembang dari satu sel dan bagaimana sel yang sehat dapat mengganti kerusakan sel pada mahluk hidup. Hal yang menjanjikan ini membuat para peneliti mengembangkan lebih jauh kemungkinan pengobatan menggunakan sel punca, untuk suatu penyakit yang dikenal dengan pengobatan regeneratif. Sel punca mempunyai dua karakteristik penting, yang membedakan mereka dari jenis sel lainnya.
Penambatan Molekuler Senyawa Bioaktif Tanaman Metang terhadap Reseptor Estrogen Alfa sebagai Antikanker Agus Saputra; Ietje Wientarsih; Lina Novianti Sutardi; Mohamad Rafi; Silmi Mariya
Acta VETERINARIA Indonesiana Vol. 12 No. 3 (2024): November 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.3.203-210

Abstract

Breast cancer is a common health problem in women and causes a high mortality rate. Cancer cells can grow and metastasize to other organs and related to estrogen and estrogen receptor alpha (ERα). The search for plant-based bioactive compounds that are antagonistic to ERα is currently being carried out using an in silico approach. Lunasia amara blanco is a medicinal plant that contains quinolone alkaloid compounds and has been known to inhibit DNA Topoisomerase II. This study aims to predict the interaction of lunacrine, graveoline, lunine, lunacridine, and lumarine compounds on the estrogen receptor alpha (ERα) (PDB 1SJ0) by in silico method. Docking will be done with PyRex 0.8 and the result visualization will be done with the BIOVIA Discovery Studio Visualizer. Base on the results of molecular docking, graveoline and lunine compounds have bond energies of -8.4 and -8.0 kcal/mol, approaching the native ligan of tamoxifen, which is -9.7 kcal/mol. The type of interaction with amino acids affects the bond energy. The amino acid residues that formed interactions with all the test compounds were Ala350, Leu387, Met388, Phe404, and Ile424. The stability of the binding of tamoxifen and graveoline is also thought to be due to the amino acids Asp351 and Cys530. The interaction of the two amino acids is not found in other compounds and the interactions formed are in the form of hydrogen bonds or hydrophobicity. Lunamarine has the lowest bond energy and make interactions with different amino acids
Co-Authors Agus saputra Deded Sarip Nawawi Devi Armilasari Dewi, Fitriya Nur Annisa Diah Iskandriati Dondin Sajuthi Dwi Ramadhani I Wayan Darmawan IETJE WIENTARSIH Iin Indriawati Iin Kurnia Hasan Basri Irma Herawati Suparto Lina Novianti Sutardi M. Rafi MARYATI SURYA Pamungkas, Joko PERMANAWATI PERMANAWATI Rita Kartika Sari Teja Kisnanto Uus Saepuloh