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Universitas Negeri Yogyakarta
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Journal : INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES

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INHIBITION OF BIFIDOBACTERIUM sp ISOLATED FROM INFANTS FECES TOWARDS ADHESION OF SALMONELLA TYPHI ON BALB/c MICE ENTEROCYTE Sukrama, I D. M.; Sukardika, K.; -, Sumarno; Mantik Astawa, N.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 4, No. 1 Januari 2010
Publisher : Udayana University

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Abstract

Diarrhea, up to the recent year remains a cause of high morbidity and mortalityworldwide, especially in developing countries including Indonesia. Research concerning ofmanagement, prevention, and medication of the disease have been continually improved. Theaim of this research is searching Bifidobacterium sp isolated from infants feces. ThisBifidobacterium was then applied as an anti-adhesion of Salmonella typhi in the hope to gain acure of diarrhea. This research employed two research designs, namely descriptive explorationand true experimental. Exploration was applied in order to obtain isolation and characterizationof Bifidobacterium isolated from infants feces. Adherence ability of this Bifidobacterium sptowards Salmonella typhi adhesion on mice entherocyte was then carried out by applyingRandomized Posttest-Only Control Group Design. In this research, average Bifidobacterium spadhesion index of 1950 on entherocyte was obtained. In simple word, there are 19.5Bifidobacteria adhere to any single entherocyte cell. This adhesion index value is highercompare to Salmonella typhi adhesion of 1504. Conclusions that can be drawn from this researchare the finding of Bifidobacterium sp isolated from infants feces. This Bifidobacterium sp has anability to inhibit adhesion of Salmonella typhi on BALB/c mice enterocyte. Future work that canbe carried out are further researches concerning whether these bacteria have an ability to inhibitadherence of other pathogen bacteria. More over, searching of cell wall adhesin ofBifidobacterium sp that can be used as a replacement of life probiotic bacteria is also a greatinterest of research to be carried out.
CHARACTERISTICS OF PILI HEMAGLUTININ PROTEIN AND ITS ROLE IN THE PATHOGENESIS OF URINARY TRACT INFECTION WITH UROPATHOGENIC ESCHERICHIA COLI Sudhana, I Wayan; Suwitra, Ketut; -, Sumarno
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 3, No. 2 Juli 2009
Publisher : Udayana University

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Abstract

Urinary tract infection (UTI) is one of the most common infectious diseasesencountered in the community. The bacteria most frequently implicated as the causes ofUTI are the Gram-negative bacteria, especially Escherechia coli. Early phase of thepathogenesis of the infection constitutes adhesion of bacteria onto the epithelial cells ofurinary tract. SDS-PAGE examination was carried out to investigate molecule ofhemagglutinin protein and hemagglutination (HA) test continued by adhesion andinhibited adhesion tests.The observation of molecular weight of pili E. coli protein molecule byexplorative examination using SDS-PAGE showed on E. coli strip that the mostprominent molecular weights (MW) of the proteins were 61 kDa, 37 kDa, 30 kDa, and 20kDa. Purification by electro-elusion was done to proteins of the MW of 20 kDa, 37 kDaand 61 kDa. The HA test results indicated the 61 kDa, 37 kDa, and 20 kDa werehemagglutinin proteins and one which subsequently applied as sub unit proteins ofUropathogenic E. coli (UPEC) was the 37 kDa. Adhesion test of UPEC bacteria carriedout on urinary bladder epithelial cells of rabbit showed a significant correlation betweenadhesion index and various doses of subunit proteins of UPEC 37 kDa as coat of urinarybladder epithelial cells. Spearman rank correlation test and regression/non regressionanalysis confirmed an exponential decrease of UPEC adhesion to urinary tract epithelialcells as a response to the increasing doses of coating protein. This finding points out thatpili hemagglutinin protein subunit of UPEC 37 kDa was an adhesive molecule. Pilihemagglutinin protein subunit of UPEC 37 kDa is an adhesive molecule, which plays arole in adherence of UPEC to epithelial cells of urinary bladder at the early pathogenesisof urinary tract infection.
Co-Authors , SUFIATIN - Indrianawati AGUSTRI AYU, WULAN AHMAD AZHAR, EMIR AROFIAH, NANDA AYU MAGFIROH, DIAH BAGUS ROHMAN PRADIPTA, ADITYA Cahyono, Erwin Budi Eka, Ervina Eka, Ervina EKO SUBAGTIO, MUHAMMAD Ernawati, Iin GAGAH PRAMA WIBAWA, MAS Gunistiyo, Gunistiyo Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Herlina Herlina I Dewa Made Sukrama I NYOMAN MANTIK ASTAWA I Wayan Sudhana IVAN NUR YASIN, MOH. Iwang Yusuf JANNAH, MIFTAKUL K. Sukardika Ketut Suwitra MAULIDINA, HIDAYAH Medina, Farah Mulyanto . MUSTIFARIS NUGROHO, AGUNG Nahara Amari, Syarifa Nahara Amari, Syarifa NAHDIA, IMROATUN Nani Zuraida NUR PUTRIATI, TRI Prasetiyo . Purwo Subekti PUTRI DANA ISWARA, LARAS RAMADHANI, SALSABILA Ramadhani, Syifa Eka RAVANA PUTRI, RISKA SAPUTRI, DIAN SETIAWAN, HANIF shamsu, nur SURYA ARIFIAN, DWI