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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Biologi Universitas Andalas
Dahelmi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Authentication of Barbonymus spp. From Lake Singkarak Using DNA Barcoding: Authentication of Barbonymus spp. From Lake Singkarak using DNA Barcoding Salis, Viola Mutiara; Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah; Aadrean; Dahelmi
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.15

Abstract

The local community distinguishes between Barbonymus belinka (Balingka) and Barbonymus schwanefeldii (Kapiek) in Lake Singkarak based on size due to the morphological similarities between the two species. From previous reports, B. belinka (Balingka) is a fish endemic to Lake Singkarak, West Sumatra, while B. schwanefeldii has a wider distribution, including Sumatra, Kalimantan, and Java. Consequently, molecular identification is necessary to discern between the species and to understand the DNA barcode characteristics of fish belonging to the genus Barbonymus in Lake Singkarak. One molecular technique utilized for species identification is DNA barcoding, which focuses on the COI (Cytochrome Oxidase Subunit I) gene. Liver tissue samples from Balingka and Kapiek fish from Lake Singkarak were used in the study. Based on 585 bp of COI gene sequences and 30 comparison sequences from BOLD system and GenBank NCBI, seven samples from Lake Singkarak show a genetic distance of 0–1.2% from B. schwanefeldii populations elsewhere, with 15 differing nucleotide bases. Moreover, samples from Lake Singkarak show a genetic distance of 7.7–8.2% from B. belinka in the BOLD system from Aceh, with 42 differing nucleotide bases. Furthermore, two specific bases are present in B. schwanefeldii from Lake Singkarak. Based on the results of this research, it is known that all samples from Lake Singkarak, including Balingka and Kapiek, belong to the same species, namely B. schwanefeldii.
A Rapid Assessment: Insect Diversity Around the Stingless Bee Colony in Edufarm Universitas Andalas Ilmi, Miftahul; Henny Herwina; Dahelmi; Alponsin; Jasmi; Rusdimansyah
Jurnal Biologi Universitas Andalas Vol 13 No 01 (2025)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.13.01.29-35.2025

Abstract

Insects dominate the animal kingdom, comprising nearly 80% of all animal species, with around 250,000 species found in Indonesia. Among these, stingless bees are a diverse group with over 60 genera. Some stingless bees are cultivated in meliponiculture, practiced around homes and plantations. This study aimed to assess insect diversity near stingless bee colonies in the Education Farm (Edufarm), Universitas Andalas, through a rapid assessment conducted over two days in May 2024. A transect was established with three U-shaped sub-transects surrounding a stingless bee colony. The modify quadra protocol sampling (honey bait traps, leaf litter, soil cores, and hand collecting) was used around the colony. The study identified 1153 individuals from 6 orders, 9 families, and 15 species of insects, with Formicidae from Hymenoptera being the most dominant. This research offers valuable insights into insect diversity around stingless bee colonies at Edufarm and can inform better management strategies for stingless bees and the ecological structure of surrounding environments.
Co-Authors Aadrean Alponsin Dewi Imelda Roesma Djong Hon Tjong Henny Herwina Jasmi Miftahul Ilmi, Miftahul Rusdimansyah Salis, Viola Mutiara Syaifullah