Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal SAINSTIS El-QUDWAH Biogenesis: Jurnal Ilmiah Biologi INDONESIAN JOURNAL OF PHARMACY Jurnal Biodjati Indonesian Journal of Cardiology Berkala Penelitian Hayati
Retno Susilowati
Department Of Biology, Faculty Of Science And Technology, Maulana Malik Ibrahim Islamic State University Of Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Published : 9 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 9 Documents
Search

 1 
Metabolite Profiling of Eleutherine palmifolia (L.) Merr. By HPTLC-Densitometry and its Correlation with Anticancer Activities and In Vitro Toxicity Mutiah, Roihatul; Hadya, Chorida Muhjatul; Ma'arif Z.A, Burhan; Bhagawan, Weka Sidha; Annisa, Rahmi; Indrawijaya, Yen Yen Ari; Huwaida, Fadhila Isma; Ramadhani D.A., Ria; Susilowati, Retno; Taufik, Imam
Indonesian Journal of Pharmacy Vol 30 No 3, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1608.73 KB) | DOI: 10.14499/indonesianjpharm30iss3pp157

Abstract

Eleutherine palmifolia (L.) Merr. (E. palmifolia) is a plant that used as raw material for herbal medicines. The difference of growing location the medicinal plants affects the difference of metabolite content, It also affects the pharmacological activity and toxicity of the plant. This study aims to determine the profile of metabolites (metabolite fingerprinting), anticancer profiles, toxicity/safety profiles from several different regions in Indonesia for the purpose of authentication, efficacy, safety and quality control of these plants. Samples were sampled from 6 different locations in Indonesia, they were West Java, Central Java, East Java, East Borneo, Central Borneo, and South Borneo. Metabolite fingerprinting was determined by HPTLC-densitometry method, the profile of anticancer activity and toxicity was known by the MTT-ELISA method. The difference between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Principal Component Analysis (PCA) and Hierarchical Component Analysis (HCA). Whereas the relation between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Partial Least Square (PLS). The results of the metabolite profile analysis with PCA showed that E. palmifolia from 6 different locations gave different metabolite profiles and there were 3 metabolites that had a significant effect on cluster formation. samples are Rf 055, 059, and 044. The results of the HCA analysis showed that the 6 regions are in one cluster the same one. The analysis of PLS-DA showed that the metabolites for anticancer activity are found in Rf 059, 076, 093 and Rf 034. While the metabolites for toxicity are Rf 002, Rf 044 and Rf 059.
EVALUASI POTENSI HEPAR SEBAGAI SUMBER ENZIM PLATELET ACTIVATING FACTOR ACETYLHIDROLASE (PAF-AH) PADA PROSES ATEROGENESIS Retno Susilowati
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (839.521 KB) | DOI: 10.18860/sains.v0i0.2304

Abstract

The atherogenesis is a fairly complicated process that deposit cholesterol in wall of arterial blood. Pathogenic atherosclerotic plaque formation requires very long time and it is often called as the silent killer. PAF-AH is a proatherogenic enzyme whose levels in serum rise rapidly since the beginning of the process atherogenesis. The sources of PAF-AH other than macrophages in the atherosklerostic plaque not been widely studied. The liver is the organ where the place of lipid metabolism and susceptible to oxidative stress. Oxidized Lipids serve as a stimulator of the synthesis of PAF-AH, so it needs to be explored its potential as a source of PAF-AH. This study aims to evaluate the liver as the source of PAF-AH on atherogenesis process that occurs in mice. This research was conducted based on post Test Control Design.This research is to observe PAF-AH levels in the lysates serum and liver. Histological observations of the presence of PAF-AH enzymes in liver tissue with the Immunohistochemistry are also observed in normocholesterol mice than hypercholesterolemic rats’ diet for 30 days. Data were analyzed with Anova test and correlation analysis. The results showed that hyperclolesterolemia rats had higher levels of PAF-AH in serum than normocholesterolemia mice (408.35 ± 142.23 218.93 ±106.07 ng /ml). Hypercholesterolemia mice had higher levels of PAF-AH in the liver lysate than normocholesterol mice (554.81 ± 143.33 275.60 ± 89.68 ng/ml). PAF-AH levels in serum correlate with levels in serum, PAF-AH liver lysate is more than the levels in serum. Immunohistochemistry show that enzyme PAF- AH found in liver tissue constituent cells.
HABBATUS SAUDAH SEBAGAI AMELIORAN FUNGSI PANKREAS PADA MENCIT DIABETES Retno Susilowati
El-QUDWAH El-Qudwah (04-2006)
Publisher : lp2m-uin malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.638 KB)

Abstract

The aims of this study are to inventory potential of Habbatus Saudah (Nigella sativa L.) to maintain pancreas B Cells, reduced blood glucose, and increases body weight diabetic mellitus patient induced by streptozotocin (STZ). STZ as antibiotic agent has a sitotoxic effect and light carcinogenic. Injection STZ cause damages Beta cells and reduce insulin secretion.  Fifteen mice 60 days old with 30-31 grams bodyweight are separate into 4 groups: Diabetic group (D), treatment group (DH), treatment control (KH) and Blanco control (K) respectively. Diabetic rats are subcutaneous injected by streptozotocin (STZ) with dose 50 mg/bw. Treatment group added habbatus saudah seed crushed 4 g/kg feed ad libitum a week after injection, for 30 days treatment. The result shows that habbatus saudah has a potential pancreas ameliorant in mice, reduce blood glucose (p 0, 01) and increase mice bodyweight (p 0, 05). Key words: Mice, Ameliorant, Diabetic, blood glucose, bodyweight, streptozotocin, Habbatus saudah, Nigella sativa L.
PENGARUH AKUT DIET ATEROGENIK TERHADAP PROFIL LIPID DAN LIPOPROTEIN-PHOSPHOLIPASE A2 Retno Susilowati
El-QUDWAH El-Qudwah (10-2010)
Publisher : lp2m-uin malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.494 KB)

Abstract

Atherosclerosis is the primary cause of vascular disease is often fatal as coronary heart disease and stroke. The disease is common in chronic and critical stage, this is due partly because the method of early detection of atherosclerotic disease is less effective and clinical symptoms emerged after at this stage even more critical. Atherosclerosis is a chronic disease and occurs in old age, this causes most of atherosclerosis research conducted only on the chronic exposure for 3 months. Therefore needs to be an early marker of atherosclerosis through the observation of the major risk factors of atherosclerosis (lipid profile) in the early exposure (≤ 1 month or less) at a young age. The enzyme Lp-PLA2 is a non-conventional risk factors found in the latest, the activity produces sdLDL with the result also need to be assessed in the presence acute exposure of diet hypercholesterolemic. The research is an experimental research using mice (Mus musculus) aged 2 months with weight on average 200g, divided into two groups namely the control and treatment group of atherogenic diet. Diet treatment was given 4 weeks. At the end of the study, total cholesterol, triglycerides, LDL, HDL and apoB levels to be measured. To find the difference of these parameters, the data are tested using the test T. In addition, related atherogenic diet influence on plasma Lp-PLA2 conducted literature review and descriptive analysis of Lp-PLA2. The results showed that diet affects lipoprotein  metabolism characterized by very significance effects (p 0.01) increased levels of total cholesterol, triglycerides, LDL and significantly (p 0.05) lower HDL cholesterol in the blood of mice at exposure for 4 weeks. Atherogenic diet has not shown its impact on apoB levels, which allegedly also had influence on levels of Lp-PLA2 in plasma 4 weeks, but based on literature studies, and levels of Lp-PLA2 activity has increased the exposure of cholesterol and diabetes for a month.
Indonesia Black Cumin (Nigella sativa L.) Seeds Extract as Ameliorant Reproductive Function in Type-2 Diabetes Mellitus Retno Susilowati; Nailirrohmah Hidayatin; Amalia Rizka Diana; Tri Kustono Adi
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11973

Abstract

Diabetes initiates augmented damage in levels of nuclear and mitochondrial DNA in males. As a result, the sperm DNA is affected, as well, leading to lower levels of fecundity and influencing reproductive health. This study aims to improve male reproductive function and oxidative stress status in diabetic rats. Combination High Fat Diet (HFD) and Streptozotocin (STZ) injection 30 mg/kg intraperitoneally are the initiations of DM-2 for 14 weeks. Treat therapy using 80% ethanol extract of black cumin seeds takes five weeks. Based on parametric test on ANOVA test results followed by Duncan Multiple Range Tests on the concentration, abnormalities of spermatozoa and Super Oxide Dismutase (SOD) effects and the Kruskal-Wallis test followed by the Mann-Whitney test on non-parametric data of the spermatozoa motility and Malondialdehyde (MDA) levels, showed that obtaining black cumin dose 48 is an effective dose in improving sperm quality and stress oxidation level. It has the same effect with metformin, even has MDA level less than normal rats. This study finds out Ns-48 is an effective dose of ethanol extract of black cumin seeds to improving spermatozoa quality and stress oxidation level, so that it becomes valuable information for research related to the improvement of reproductive function in diabetes mellitus disease.
Korelasi Antara Kadar Lp-PLA2, MDA, F2-Isp di Serum dan Jaringan Aorta dengan Jumlah Sel Busa dalam Proses Aterogenesis pada Tikus Wistar Retno Susilowati; Djanggan Sargowo; Rasjad Indra; Askandar Tjokroprawiro; Sri Widyarti
Jurnal Kardiologi Indonesia Vol. 33, No. 4 Oktober - Desember 2012
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v33i4.288

Abstract

Background. Atherogenesiswas initiated by cholesterol deposits on foam cell in sub intimae of blood vessel stress oxidation. Atherogenesis in non- hypercolesterolemiausually undergoes an increased level of Lp-PLA2. It, therefore, needs to evaluate the role of Lp-PLA2in the foam cell formation. Aims. To explain the role of Lp-PLA2 in the foam cell forming, to correlate the level of Lp-PLA2 ,MDA, F2-Isp in aorta with foam cell number (FCs) as well as to correlate the level of Lp-PLA2 , MDA, F2-Isp contents in serumwith their contents in aorta and the correlation with FCs.Methods. 30 rats aged 2 months, with their weight averaging from 150-200g,were divided into the control group and the treatment one where the latterwas fed hyperlidemia for 2,8 and 12 weeks. The measurement level of LDL-C,MDA, F2-isp and Lp-PLA2in serum was performedas well as aorta and FCs. Data was analysed using anova, t-Test, path analysis and correlation. Results. Research result indicated that: (1) The level of MDA(a), F2-Isp(s) and Lp-PLA2(s) positively correlated with FCs, Lp-PLA(s)having the highestcorrelation value. (2) Lp-PLA2(a),MDA(s) and F2-Isp(s,a)did not correlate withFCs. (3) There was a positive correlation between Lp-PLA2 with MDA andF2-Isp in both serum and aorta.Conclusion. The enzyme of Lp-PLA2 acts as an activator in forming thefoam cell with stimulated stress oxidation.
Three Dangerous Loops Of Lipoproteine-Associated Phospholipase A2 Activity On Increasing LDL Aterogenecity Retno Susilowati; Djanggan Sargowo; Askandar Tjokroprawiro
Jurnal Kardiologi Indonesia Vol 40 No 3 (2019): Indonesian Journal of Cardiology: July-September 2019
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v40i3.680

Abstract

Background. Hypercholesterolemia is a major classic risk factor for cardiovascular disease, but there are 35%-40% cases of cardiovascular patients have a normal cholesterol levels. Lp-PLA2 is an enzyme that produced and secreted by macrophages as a response to the lipid peroxide formation, especially the platelet activating factor compound and phosphocholine peroxide. Lp-PLA2 is correlated with classic risk factor of cardiovascular disease, although that correlation with number of foam cell at early stage of atherosclerosis is not clear yet. This study aims to determine whether Lp-PLA2 levels correlated with classic risk factors of atherosclerosis and the number of foam cell, and the role of Lp-PLA2 enzyme in foam cells formation.Methods. This study observes the change of Lp-PLA2, F2-Isp, MDA, TC, LDL, HDL levels in rat serum at 3 levels of early atherogenesis, Ath-I, Ath-II and Ath-III were made on the number of foam cells. The number of cells was observed on all aortic cross sectional surfaces, using the Oil-Red-O staining. The LDL-C content was measure using the Fiedwall formula, MDA content was measure by using TBA-test, the observe of F2-isoprostane and Lp-PLA2 followed the procedure Elisa methods. Results. Anova test results among the 3 initial atherosclerotic levels showed a very significant difference (p<0.01) on Lp-PLA2 plasma content. The LSD test results represented an increase in Lp-PLA2 enzyme levels significantly since AthII stage. Path analysis refers that correlation value between the Lp-PLA2and the number of foam cell (r=0.48) were higher than that of the LDL (r=0.42), was neither correlated with MDA nor F2-Isp, the highest correlation occurred between Lp-PLA2 and LDL compared to the others parameters (r = 0.58). Path analysis also showed no correlation between cell numbers with MDA and F2-Isp, but LDL levels are correlated significantly with of oxidative stress markers MDA levels (r = 0.32) and correlated very significantly with F2-Isp (r = 0.69).Conclussion. It can be concluded that elevated levels of Lp-PLA2 increase atherogenecity of LDL, due to increased inflammation, stress oxidation and elevated levels of Lp-PLA2 itself, wich are interconnected with proatherogenic loops.
PEMANFAATAN EKSTRAK JINTAN HITAM UNTUK MENURUNKAN KADAR ENZIM LP-PLA2 SEBAGAI KANDIDAT PENGOBATAN ATEROSKLEROSIS Retno Susilowati; Evika Sandi Savitri; Kholifah Holil
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.795 KB) | DOI: 10.23869/154

Abstract

Ox-LDL deposits in the sub-endothelial easily occur in individuals who have hyperlipidemia accompanied with oxidative stress. The enzyme Lp-PLA2 is an enzyme marker of three proaterogenik conditions, those are hyperlipidemia, oxidative stress and inflammation. Black cumin seeds (Nigella sativa L.) have antioxidants ingredient that can inhibit lipid peroxidation, and expected to inhibit atherosclerosis through decreased levels of the enzyme Lp-PLA2 and F2-Isp. This study used posttest only control group design using experimental animals Rattus norvegicus Wistar males. The study analysied the number of foam cells, lipid profi le, Lp-PLA2 andF2-Isp plasma levels in hyperlipidemia rats were given extracts of black cumin seeds with 3 different doses (0; 3.6 and 7.2 mg / kg). The results showed that the extract of black cumin seeds can decrease serum levels of Lp-PLA2, tend to reduce the formation of foam cells,but not signifi cant to decreasing levels of F2-Isp. Black cumin seeds extract can decrease cholesterol, TG, LDL and increase HDL in the blood serum. The extract of black cumin seeds is anti atherogenic by reducing the enzyme Lp-PLA2 and improve lipid profi les.
Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice Retno Susilowati; Ulfa Maynisa Rohmanningrum
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.19968

Abstract

High levels of free radicals in diabetic wounds often cause chronic inflammation. Nigella sativa and Trigonella foenum-graecum seed extracts are rich in antioxidants and have anti-inflammatory effects. Trigonella foenum-graecum seed extracts can also accelerate the reepithelialization process in wound healing. This study aimed to determine the combination of ethanol extract of Nigella sativa and Trigonella foenum-graecum seeds on wound length and the number of neutrophils, macrophages, fibroblasts, and collagen density in the incision wound of diabetic mice induced by streptozotocin. T. This study used three types of controls, namely Normal (Normal mice,  HPMC 3%),  DM (Diabetic mice, HPMC 3%), and Iodine (Diabetic mice, Povidone Iodine 10%). Topical treatment with 70% ethanol extract ointment combined with Nigella sativa and Trigonella foenum-graecum in various variations, namely N (20%:0%), T (0%:10%), NT (10%:5%), Observations of the wound healing process were carried out on 3rd, 7th and 14th day. Data were analyzed using the One Way ANOVA and Duncan Multiple Range Test. The study's results by observing the morphology of the wound length on day 7th day showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum resulted in the shortest wound, which was significantly different from the wound in untreated diabetic mice. In addition, diabetic wounds without treatment showed infection, and on the contrary, the infection did not occur in wounds treated with a combination of extracts of Nigella sativa and Trigonella foenum-graecum. The results of wound histology observations showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum showed a decrease in inflammation which was indicated by a significant decrease in the number of neutrophil cells, macrophages, and accelerated reepithelialization of wound healing as indicated by a significant increase in the number of fibroblasts and collagen density since the third day of treatment.
Co-Authors Amalia Rizka Diana Askandar Tjokroprawiro Djanggan Sargowo Djanggan Sargowo Evika Sandi Savitri Hadya, Chorida Muhjatul Huwaida, Fadhila Isma Kholifah Holil Ma'arif Z.A, Burhan Nailirrohmah Hidayatin Rahmi Annisa Ramadhani D.A., Ria Rasjad Indra Roihatul Mutiah Sri Widyarti Taufik, Imam Tri Kustono Adi Ulfa Maynisa Rohmanningrum Weka Sidha Bhagawan Yen Yen Ari Indrawijaya