Article Per Year (5 Year)
p-Index From 2020 - 2025
1.532
P-Index
This Author published in this journals
All Journal Jurnal Dedikasi METAMORFOSA Journal of Biological Sciences AL KAUNIYAH El-Hayah : Jurnal Biologi Journal of Islamic Pharmacy AL QUDS : Jurnal Studi Alquran dan Hadis Jurnal Biodjati Jurnal SOLMA Bioinformatics and Biomedical Research Journal Berkala Penelitian Hayati Es-Syajar:Journal of Islam, Science and Technology Integration Journal of Research on Community Engagement
Kholifah Holil
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

Published : 18 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 18 Documents
Search

 1   2 
PEMBUA PEMBUATAN AN PREP PREPARA ARA ARAT SEBAGAI MEDIA PENDIDIKAN P PADA ADA BIDANG STUDI BIOLOGI Holil, Kholifah; M.Kes,, Drs. Ainur Rofieq,; ,M.Kes, Dra. Sri Wahyuni
Jurnal Dedikasi Vol 1, No 1 (2004): Mei
Publisher : Direktorat Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.188 KB) | DOI: 10.22219/dedikasi.v1i1.924

Abstract

Fungsi preparat dalam bidang studi biologi di SD, SMP dan SMU sangatpenting untuk memberikan pengetahuan dan pengalaman langsung perihal tubuhatau bagian organ tertentu pada hewan maupun tumbuhan. Contoh sederhanamisalnya; ketika guru menjelaskan bahwa jaringan darah tersusun atas sel darahmerah dan berbagai sel darah putih. Kalau hanya dengan menjelaskan saja, berartiguru mengajak siswa berpikir abstrak tentang jaringan darah. Hasil pembelajaranini tidak memberikan pengalaman langsung sehingga siswa mudah lupa dan mungkinsekali terjadi akumulasi konsep yang berbeda-beda tentang jaringan darah.Semestinya guru dengan bantuan mikroskop dapat menunjukkan preparat jaringandarah dengan menunjukkan sel-sel darah yang terlihat. Dengan cara ini merekapunya pengalaman langsung dan memiliki konsep yang sama tentang komponenjaringan darah.Dasar pertimbangan dan alasan-alasan itulah yang menjadi landasan awal bagimunculnya gagasan pembuatan preparat organisme untuk bidang studi biologi.Alasan lain yang mendukung gagasan adalah adanya alat seksi jaringan (mikrotom)didalam Laboratorium Biologi UMM yang hanya dimanfaatkan untuk keperluanpraktikum reguler.
Analisis Fitokimia dan Aktivitas Antioksidan Ekstrak Daun Kesambi (Schleira oleosa) Metode DPPH Holil, Kholifah; Griana, Tias Pramesti
Journal of Islamic Pharmacy Vol 5, No 1 (2020): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v5i1.9387

Abstract

Kesambi (Schleichera oleosa) is one of the forest plants from Indonesia that has potential as medicine. Kesambi has active compounds that act as antioxidants. The purpose of this study was to determine the potential of kesambi leaf as an antioxidant using the DPPH method. Samples of kesambi leaf were extracted in methanol, ethanol, and water solvents by the maceration method. The class of compounds contained in kesambi leaf was tested by the colour reaction test method for alkaloids, flavonoids, triterpenoids, phenols, steroids, saponins, and tannins. Antioxidant activity was tested by the DPPH method to determine IC50 values. Based on the results of the colour reaction test showed that methanol and ethanol solvents were able to bind 6 classes of compounds (alkaloids, flavonoids, triterpenoid, phenols, saponins, and tannins) and water solvents were only able to bind 5 classes of compounds (flavonoids, triterpenoid, phenols, saponins, and tannins) contained in kesambi leaf. The results of antioxidant activity tests using the DPPH method showed the lowest IC50 value was methanol extract (16,12µg/ml) compared to ethanol extract (20,43µg/ml) and water (904,28µg/ml). It was concluded that the leaf extract of kesambi has potential as an antioxidant and which has the best antioxidant ability was extracted in methanol. The class of compounds which were thought to be responsible for the antioxidant activity of the kesambi leaf extract based on the color reaction test were phenols, flavonoids and tannins.Keywords: antioxidant, kesambi, Schleichera oleosa, DPPH
PROFIL KADAR MDA PADA TUMOR KULIT MENCIT (Mus musculus) SEBAGAI AKIBAT PEMBERIAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L) DAN EKSTRAK METANOL BENALU TEH (Scurulla atropurpurea Bl. Dancer) Holil, Kholifah
El-Hayah : Jurnal Biologi Vol 4, No 1 (2013): EL-HAYAH (VOL 4, NO 1, September 2013)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v4i1.2616

Abstract

Malondialdehyde (MDA) is widely used as an indicator of lipid peroxidation. Increased levels of MDA have been associated with a variety of tumor diseases , one of is a skin tumor. To cope with this disease use several medicinal plants which are leaves of soursop (Annona muricata L) and tea parasite (Scurulla atropurpurea Bl. dancer) . This study aims to determine the profile of MDA in mouse skin tumors as a result of ethanol extract of leaves of soursop and methanol extract of tea parasite. The sample used in this study were mouse skin tumors derived from mice induced by using 7.12 - Dimethilbenz(α)Antrasen (DMBA) and ethanol extract of leaves of soursop and methanol extract of tea parasite. While the observation parameters are obtained via MDA spectrophotometer method. The result of this research showed that the levels of MDA in mouse skin tumors induced ethanol extract of leaves of soursop and methanol extract of tea parasite is different. Lowest MDA levels (34.95nmol/g) contained in the DS3 that comes from DMBA-induced mice 25μg/100μl of acetone and ethanol extracts of soursop leaf 60mg/kg
EVALUASI OOSIT KAMBING HASIL IVM SEBAGAI SALAH SATU FAKTOR PENENTU KEBERHASILAN DALAM AKTIVASI PARTENOGENESIS Holil, Kholifah
El-Hayah : Jurnal Biologi Vol 2, No 1 (2011): EL-HAYAH (VOL 2, NO 1, September 2011)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v2i1.1791

Abstract

Aktivasi partenogenesis merupakan salah satu tehnik aktivasi oosit untuk menghasilkan embrio tanpa kontribusi dari sperma. Salah satu faktor yang menentukan keberhasilan tehnik ini adalah pada ketersediaan oosit yang berkualitas. Oleh karena itu penelitian ini bertujuan untuk mengevaluasi oosit kambing hasil IVM yang dapat digunakan untuk kepentingan dalam aktivasi partenogenesis tersebut.Sampel yang digunakan dalam penelitian ini adalah oosit yang diaspirasi dari folikel ovarium kambing yang diambil dari RPH Sukun Malang. Oosit di IVM selama 24 jam dan selama 27 jam dalam medium TCM-199 yang ditambah dengan fetal bovine serum (FBS), follicle-stimulating hormone (FSH) dan lutheinizing hormone (LH) dan diinkubasi pada suhu 38,5oC, 5% CO2. Pada jam ke 24 dan jam ke 27 setelah IVM dilakukan pengamatan yang meliputi ekspansi sel-sel kumulus dan keberadaan polar body I (PB-I) pada jam ke 30. Berdasarkan hasil penelitian menunjukkan bahwa oosit kambing  yang di IVM  sampai jam ke 24 belum menunjukkan adanya ekspansi sel-sel kumulus kualitas 2 dan penampakan PB-I. Hasil tersebut berbeda dengan oosit yang di IVM sampai jam ke 27. Pada pengamatan jam ke 27 ini menunjukkan bahwa terdapat 77,87% oosit yang sel-sel kumulusnya berekspansi (kualitas 2) dan PB-I yang nampak sebesar 95,32%.  Dengan demikian maka dapat disimpulkan bahwa oosit kambing hasil IVM  baru dapat digunakan untuk keperluan lebih lanjut khususnya untuk keperluan aktivasi partenogenesis pada jam ke 27.Kata kunci: evaluasi, aktivasi partenogenesis, oosit kambing hasil IVM
EFEK VITAMIN C DALAM MEDIUM DMEM TERHADAP PERTUMBUHAN SEL PARU-PARU FETUS HAMSTER SECARA IN VITRO W, Ema Kurnia; Holil, Kholifah
El-Hayah : Jurnal Biologi Vol 3, No 1 (2012): EL-Hayah (Vol 3, No 1, September 2012)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v3i1.2219

Abstract

Penelitian ini dilakukan dengan tujuan untuk mengetahui efek vitamin C dalam medium DMEM terhadap pertumbuhan sel paru-paru fetus hamster secara in  vitro dan untuk mengetahui pada konsentrasi berapakah vitamin C dalam medium DMEM yang berpengaruh paling efektif terhadap pertumbuhan sel paru-paru fetus hamster secara in vitro.  Penelitian  ini  merupakan penelitian  eksperimental dengan    6  perlakuan  dan  2 ulangan. Masing-masing perlakuan tersebut adalah vitamin C dengan konsentrasi 0mM (kontrol), 5mM, 10mM, 15mM, 20mM, dan 25mM. Vitamin C dengan berbagai konsentrasi tersebut ditambahkan ke dalam medium DMEM dan selanjutnya digunakan untuk menumbuhkan sel paru-paru fetus hamster. Sel paru-paru fetus hamster diisolasi dari fetus hamster berumur 2 hari, ditumbuhkan dalam medium DMEM yang sudah ditambah dengan vitamin C dengan berbagai konsentrasi dan diinkubasi dalam inkubator CO2 5% pada suhu 370C selama beberapa hari sampai biakan sel-sel tersebut konfluen. Setelah konfluenbiakan sel diamati persentase konfluen, viabilitas dan abnormalitas sel. Hasil penelitian menunjukkan bahwa vitamin C dengan konsentrasi 0mM (kontrol), 5mM, 10mM, 15mM, 20mM, dan 25mM dalam medium DMEM mampu menghasilkan persentase konfluen berturut-turut 50%, 65%, 72.5%, 82.5%, 87.5%, dan 92.5% dan  persentase viabilitas sel sebesar 89%, 94.5%, 94%, 93%, 94%, dan 96.5%. Sedangkan konsentrasi vitamin C yang efektif  terhadap  pertumbuhan  sel  paru-paru  fetus  hamster  secara  in  vitro  adalah konsentrasi 25mM.
UJI ANTIOKSIDAN JAMU MADURA “EMPOT SUPER” Holil, kholifah
El-Hayah : Jurnal Biologi Vol 5, No 3 (2015): EL-HAYAH (VOL 5, NO 3,September 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i3.3097

Abstract

  The purpose of this research is to present scientific information related to madura’s herbal medicine “empot super” and herbal extract made from existing composition of the various constituent plants of empot super. Then, this research spesifically detects antioxidants activity found in herbal medicine and herbal extract expected to provide a scientific assurance either for the industry or the consumen.Samples used in this research are madura’s herbal medicine and herbal extract mixture of various simplisia those are used in the manufacture of empot super herbal medicine. To get those samples, herbal medicine is imported from madura, while the herbal extract is the production of extraction process by using ethanol. The samples are subsequently assay the antioxidants activity by using DPPH method at 571nm.The final result of this research indicates the existance of IC50 proportion in herbal medicine that is 34,11mg/ml and 45,44mg/ml in herbal extract. Indeed, in the assay of herbal medicine and herbal extract classified as having a very strong antioxidants activity
Cytotoxicity Test on Breast Cancer Cell Lines T-47D treated with Pisang Kepok Peel Extract (Musa balbisiana) Wardati, Fauchil; Holil, Kholifah; Syarifah, Umaiyatus
Bioinformatics and Biomedical Research Journal Vol. 1 No. 3 (2018): Volume 1 Issue 3
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Introduction: Utilization of natural ingredients such fruit as a breast cancer treatment agent is beginning to be widely studied by scientists. The goal is to reduce the side effects of chemotherapy agents. One of the potential natural ingredients as anticancer is Pisang Kepok peel (Musa balbisiana). This kind of banana contains various phytochemical compounds and high antioxidant activity that can induce cancer cell apoptosis. The purpose of this research is to know the IC50 value of Pisang Kepok peel extract thus can be known its potential as an anticancer agent. The lower of IC50 value is more cytotoxic. Method: This research is experimentally done by using CRD (Completely Randomized Design). The stages of this research are extraction and cytotoxic test (MTT assay). The extraction method was used maceration (ethanol 95%). The cytotoxic test was performed by giving Pisang Kepok unripe peel extract on the T-47D cell lines, then read the absorbance with ELISA reader at ? 595 nm and analyzed by SPSS Probit. Conclusions: Based on this results, it can be concluded that Pisang Kepok peel extract has moderate cytotoxic to T-47D cell lines, therefore indicate its potential as a candidate for breast cancer chemoprevention agents, especially at high concentrations (>250 ?g/mL).
PEMBUA PEMBUATAN AN PREP PREPARA ARA ARAT SEBAGAI MEDIA PENDIDIKAN P PADA ADA BIDANG STUDI BIOLOGI Kholifah Holil; Drs. Ainur Rofieq, M.Kes,; Dra. Sri Wahyuni ,M.Kes
Jurnal Dedikasi Vol. 1 No. 1 (2004): Mei
Publisher : Direktorat Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/dedikasi.v1i1.924

Abstract

Fungsi preparat dalam bidang studi biologi di SD, SMP dan SMU sangatpenting untuk memberikan pengetahuan dan pengalaman langsung perihal tubuhatau bagian organ tertentu pada hewan maupun tumbuhan. Contoh sederhanamisalnya; ketika guru menjelaskan bahwa jaringan darah tersusun atas sel darahmerah dan berbagai sel darah putih. Kalau hanya dengan menjelaskan saja, berartiguru mengajak siswa berpikir abstrak tentang jaringan darah. Hasil pembelajaranini tidak memberikan pengalaman langsung sehingga siswa mudah lupa dan mungkinsekali terjadi akumulasi konsep yang berbeda-beda tentang jaringan darah.Semestinya guru dengan bantuan mikroskop dapat menunjukkan preparat jaringandarah dengan menunjukkan sel-sel darah yang terlihat. Dengan cara ini merekapunya pengalaman langsung dan memiliki konsep yang sama tentang komponenjaringan darah.Dasar pertimbangan dan alasan-alasan itulah yang menjadi landasan awal bagimunculnya gagasan pembuatan preparat organisme untuk bidang studi biologi.Alasan lain yang mendukung gagasan adalah adanya alat seksi jaringan (mikrotom)didalam Laboratorium Biologi UMM yang hanya dimanfaatkan untuk keperluanpraktikum reguler.
PEMANFAATAN EKSTRAK JINTAN HITAM UNTUK MENURUNKAN KADAR ENZIM LP-PLA2 SEBAGAI KANDIDAT PENGOBATAN ATEROSKLEROSIS Retno Susilowati; Evika Sandi Savitri; Kholifah Holil
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.795 KB) | DOI: 10.23869/154

Abstract

Ox-LDL deposits in the sub-endothelial easily occur in individuals who have hyperlipidemia accompanied with oxidative stress. The enzyme Lp-PLA2 is an enzyme marker of three proaterogenik conditions, those are hyperlipidemia, oxidative stress and inflammation. Black cumin seeds (Nigella sativa L.) have antioxidants ingredient that can inhibit lipid peroxidation, and expected to inhibit atherosclerosis through decreased levels of the enzyme Lp-PLA2 and F2-Isp. This study used posttest only control group design using experimental animals Rattus norvegicus Wistar males. The study analysied the number of foam cells, lipid profi le, Lp-PLA2 andF2-Isp plasma levels in hyperlipidemia rats were given extracts of black cumin seeds with 3 different doses (0; 3.6 and 7.2 mg / kg). The results showed that the extract of black cumin seeds can decrease serum levels of Lp-PLA2, tend to reduce the formation of foam cells,but not signifi cant to decreasing levels of F2-Isp. Black cumin seeds extract can decrease cholesterol, TG, LDL and increase HDL in the blood serum. The extract of black cumin seeds is anti atherogenic by reducing the enzyme Lp-PLA2 and improve lipid profi les.
Phytochemistry Screening and Antioxidant Activities of Extract Pomegranate, Grape, Fig, and Olive in the Various Solvent Evika Sandi Savitri; Kholifah Holil; Ruri Siti Resmisari
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.13424

Abstract

The active compounds of grape, pomegranate, olive, and fig have anthocyanins that potential as antioxidant are flavonoids. Flavonoids have potential as antioxidant  to prevent and therapy various oxidative stress and related diseases. This study aimed to examine the antioxidant activity of a combination of pomegranate, grape, fig, and olive extracts using the DPPH (diphenyl-2-picrylhydrazyl) method.  The maceration method used was maceration of dry Simplicia with methanol 95% solvent, fresh maceration with 95% methanol and dry Simplicia with 95% ethanol solvent. The results of the phytochemistry test showed several compounds found in the extract combination pomegranate, grapes, fig, and olives such as polyphenol, flavonoid, tannin, steroid/triterpenoid. The result of the antioxidant test showed the fresh maceration 95% methanol showed higher results with the IC50 of 25.22 with a potent antioxidant activity category.
Co-Authors Ahmad, Mujahiddin Ainur Rofieq Aliyansyah, Gunawan Azizatur Rahmah Dra. Sri Wahyuni ,M.Kes Dra. Sri Wahyuni ,M.Kes, Dra. Sri Wahyuni Ema Kurnia W Evika Sandi Savitri Gunawan Aliyansyah Hanifah, Lil Hartyastika, Silvi Ayuk Prima Kurniawaty Rahmah, Azizatur Retno Susilowati Retno Susilowati Ruri Siti Resmisari Tias Pramesti Griana Umaiyatus Syarifah Wardati, Fauchil