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All Journal HAYATI Journal of Biosciences SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Microbiology Indonesia Science and Technology Indonesia Eksakta : Berkala Ilmiah Bidang MIPA East Asian Journal of Multidisciplinary Research (EAJMR) Makara Journal of Science Indonesian Journal of Biomedicine and Clinical Sciences
SJATHA, FITHRIYAH
Departement Of Microbiology, Faculty Of Medicine, Universitas Indonesia, Jakarta, DKI Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Physics

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Evaluation of Tuberculosis Vaccine Candidate, pcDNA3.1-rpfD using Mycobacterial Growth Inhibition Assay (MGIA) Mifa Nurfadilah; Andriansjah Rukmana; Fithriyah Sjatha
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.1-8

Abstract

Resuscitation-promoting factor D (RpfD) is a protein involved in the resuscitation of dormant bacteria. A new tuberculosis vaccine carrying the rpfD gene has been successfully constructed, pcDNA3.1-rpfD. It was demonstrated that this vaccine exhibits cellular and humoral immune responses. Therefore, within this study, the efficacy of this new vaccine candidate was evaluated using mycobacterial growth inhibition assay (MGIA). MGIA is a functional assay that measures the complex host immune response, peripheral blood mononuclear cell (PBMC) and splenocyte from BALB/c mice against mycobacteria. With BACTECTM MGITTM 960 automated system, the effect of vaccination on bacterial growth was reported as a time to positivity (TTP) in hours. The mean of TTP from the vaccinated group (both pcDNA3.1-rpfD and BCG) was higher than the negative control group. These results suggest that pcDNA3.1-rpfD may be effective in controlling tuberculosis growth and may provide a clue for the development of the tuberculosis vaccine. In addition, despite previous evidence that IFNγ was essential for tuberculosis immunity, IFNγ (interferon gamma) production was found not to be correlated with mycobacterial inhibition. Therefore, these findings offer an alternative method to evaluate vaccine candidates than the assessment using IFNγ only.
Cloning of pe11 (LipX, Rv1169c) gene of Mycobacterium tuberculosis Beijing strain to pcDNA3.1 plasmid vector Supardi, Lulut Azmi; Rukmana, Andriansjah; Sjatha, Fithriyah
Makara Journal of Science Vol. 25, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. It is a persistent global health problem with a high mortality rate. Currently, TB is controlled by administering the Bacillus Calmette-Guerin (BCG) vaccine, but the effectiveness of its protection varies among individuals in a population. The pe/ppe gene family comprises a typical group of genes that play a role in avoiding the host immune response and inducing persistent TB infection. Based on in silico analysis, the pe11 gene has estimated immunogenicity and potential as a TB seed vaccine candidate. The pe11 gene from an Indonesian isolate of an M. tuberculosis Beijing strain was amplified by polymerase chain reaction (PCR) and inserted into the mammalian expression vector pcDNA3.1. The recombinant vector pcDNA3.1-pe11 was used to transform Top10 competent Escherichia coli. Clones from the transformation were subjected to colony PCR to confirm the direction of the insert. Sequencing was performed to confirm the correctness of the insert sequence. In this study, the pe11 gene was successfully cloned into the pcDNA3.1 vector in the correct direction to assure PE11 expression. No mutations were found in the pe11 gene insert, compared with the M. tuberculosis H37Rv sequence as the standard. A pcDNA3.1 vector containing the pe11 gene derived from an M. tuberculosis Beijing strain was successfully constructed.
Levels of TNF-α in PBMC (Peripheral Blood Mononuclear Cells) Induced by Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in vitro FITHRIYAH SJATHA; OKTIVIA CHANDRA MUSTIKA; BETI ERNAWATI DEWI; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.517 KB) | DOI: 10.5454/mi.13.2.4

Abstract

Dengue infection is a global health problem with an increasing incidence every year and now endemic in more than 100 WHO countries. Dengue infection is caused by dengue virus (DENV) which is an RNA virus with positive single strand, with ±11kb genome size encoding 3 structural proteins, 7 non-structural proteins, and two Untranslated Region (UTR). NS1 protein is known to have a very important role in the development of severe DENV infection, by the direct effect causing host cells damage and indirect effect by activating immune response to induce the secretion of excess cytokines. This study aims to evaluate whether recombinant pcNS1 plasmids which have been proven expressing recombinant NS1 proteins in previous studies is able to induce cytokine secretion from Peripheral Blood Mononuclear Cells (PBMC). Transfected Chinese Hamster Ovary-K1 (CHO-K1) cells with recombinant pcNS1 plasmid was co-cultured with PBMC from healthy donor. After 48 h post co-cultured, cell supernatant was collected and TNF-α levels and NS1 recombinant were measured by ELISA. The results showed that recombinant NS1 protein was expressed in CHO-K1 mammalian cell line and able to induce TNF-α with higher levels compared to control.
The qPCR Assay for Detecting The Presence and Relative Abundance of Pseudomonas aerugionosa and Antibiotic Resistance Gene aadA2 in Hospital Wastewater of National Reference Hospital Dr. Cipto Mangunkusumo (RSCM) Rida Tiffarent; Rosdiana Irawati; Conny Riana Tjampakasari; Fithriyah Sjatha; Windi Muziasari; Anis Karuniawati
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.977 KB) | DOI: 10.5454/mi.16.2.24-30

Abstract

Antimicrobial resistance is one of the top 10 global health threats. The hospital wastewater (HWW) potentially becomes the reservoir and dissemination of antibiotic resistance gene (ARG) and bacterial pathogens. In Indonesia, the protocol to monitor the ARGs form HWW has not been established. This study aimed to detect the presence and find the relative abundance of P. aeruginosa and aadA2 genes from Dr. RSUPN. Cipto Mangungkusumo (RSCM) inlet and outlet wastewater through qPCR assay. The primers used were supported by Resistomap. The study revealed that the qPCR assay was able to detect the Ct value of P. aeruginosa and aadA2. The aadA2 gene was found in all waste water samples, meanwhile P. aeruginosa was only found in some of inlet samples. aadA2 had the highest relative abundance and this gene’s mobility uses plasmids and integrons that potentially enhance the acquired antimicrobial resistance (AMR) mechanism. This study implicated that qPCR assay was capable to detect pathogenic bacteria and ARG, and ARG could be released to the environment even though the wastewater samples have been proceeded in wastewater treatment plants (WWTP). The qPCR assay can be used as the method to monitor the AMR status in a hospital and the spreading potency to the environment using the HWW.
Modification of Indonesian Kaolinite-Based Silica Coarse (SC) for RNA Extraction Method of SARS-CoV-2 Marintan, Marchia Marthalena; Sjatha, Fithriyah; Nurani, Dita Arifa; Krisnandi, Yuni Krisyuningsih; Sariman, Sariman
Science and Technology Indonesia Vol. 9 No. 2 (2024): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.2.325-335

Abstract

One of the strategies to overcome the COVID-19 disease is through rapid diagnostic tests using the Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) test. The RT-PCR test is a detection and quantification test of nucleic acids, initiated by the pre-analytical step of purification of the nucleic acids. Purification of nucleic acid requires silica-based materials as a solid phase-extraction matrix or column. Herein, Silica Coarse (SC) in the form of suspension and powder columns; was prepared from natural Indonesian Kaolinite as an alternative extraction column to binding RNA of SARS-CoV-2. The RNA binding and releasing ability in SC was enhanced with the support of chaotropic agents in the form of Na+ and Guanidium+ as charged balancing cations, embedded in the silicate layer inside the kaolinite framework. SC, which has been supported with Na+ and Guanidium+ respectively, then studied its physicochemical characteristics using FTIR spectroscopy, X-ray diffraction technique, scanning electron microscopy, and BET surface area and pore size measurement. This work shows that the modified SC suspension column could extract RNA of SARS-CoV-2 that amplified better in the RT PCR test than SC powder columns, with the initial Ct value of all the SARS-CoV-2 specimens in the range < 20.
Detection of human bocavirus (HBoV) in children with acute respiratory infection (ARI) during the covid-19 transition period Purnomosidi, Muhammad Abhi; Sjatha, Fithriyah; Yasmon, Andi
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 14, No 2 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v14i2.36623

Abstract

Acute respiratory infections (ARIs) are the highest cause of death in children in the world. Based on the 2018 Riset Kesehatan Dasar Nasional, ARI cases in Indonesia showed a prevalence of 4.4%, with the highest cases occurring in children. One of the new viruses first identified in 2005 in human nasopharyngeal samples is the human bocavirus (HBoV). HBoV is a single-strand DNA virus belonging to the Parvoviridae family. This study aimed to assess the prevalence of HBoV in children presenting with ARI during the transitional period of the Covid-19 era. HBoV detection was conducted using multiplex PCR and reverse hybridization methods on nasopharyngeal and oropharyngeal swab samples collected from symptomatic children. This study reported a prevalence rate of 4.94% for HBoV in 2022 and 5.04% in 2023. Furthermore, the study identified favorable detection rates for HBoV in children with ARIs as 14.81% in 2022 and 8.45% in 2023. These rates ranked 2nd and 5th highest among other pathogens detected in ARIs. Additionally, there was an increase in positive HBoV samples from 4 samples in 2022 to 6 samples in 2023, which was attributed to the relaxation of nonpharmaceutical Covid-19 interventions by mid-2022. HBoV was identified at a significant rate among children with ARI in Jakarta during the transitional phase of the Covid-19 era (2022-2023). Given its potential to induce severe ARI, HBoV necessitates heightened attention as an etiological agent.
Mobile Genetic Elements Contributing to Carbapenem Resistance in Acinetobacter baumannii: Current Insights Mardhiyah, Azzahrah Khairunnisa; Saharman, Yulia Rosa; Sjatha, Fithriyah
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 04 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss04/534

Abstract

Acinetobacter baumannii has become a major cause of hospital-acquired infections with the rapid development of resistance to multiple antibiotics, including critical carbapenems. This resistance challenge limits treatment options and increases morbidity and mortality. The genetic plasticity of A. baumannii facilitates the mobilization of resistance genes via mobile genetic elements (MGE). Addressing this crisis requires a deeper understanding of the mechanisms by which MGE propagates carbapenem resistance. This paper provides a solution by systematically reviewing recent research on the role of MGE in disseminating resistance genes. Following PRISMA guidelines, a comprehensive literature review was conducted across various databases. The review revealed that resistance mechanisms primarily involve carbapenem-hydrolyzing enzymes and MGE, such as integrons, transposons, insertion sequences, and plasmids. Notably, genes like blaOXA-23 and blaNDM are frequently mobilized by these elements, facilitating horizontal gene transfer and persistence. Understanding the mechanisms of MGE-mediated gene transfer is crucial for developing strategies to control the spread of antibiotic resistance in A. baumannii.
Distribution of blaCTX-M, blaSHV, blaTEM genes in Extended Spectrum β-Laktamase Producing Klebsiella pneumoniae from Clinical Isolates in Jakarta Engel, Yulia; Conny Riana Tjampakasari; Fithriyah Sjatha
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 26 No. 02 (2025): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol26-iss02/549

Abstract

Klebsiella pneumoniae is one of the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) due to their high level of antibiotic resistance. Ceftriaxone is one of the cephalosporin antibiotic that functions inhibit bacterial cell wall synthesis and used for treating K. pneumoniae infections. Resistance to ceftriaxone in K. pneumoniae has been widely reported, with one contributing factor being the production of β-lactamase enzymes encoded by the genes blaCTX-M, blaSHV, and blaTEM. This study characterized the presence of these genes  in 12 clinical isolates of         K. pneumoniae and analyzed their correlation with phenotypic resistance to ceftriaxone. All isolates characterized with antimicrobial susceptibility testing (AST) and disk diffusion methods to evaluate the phenotypic production of blaCTX-M, blaSHV, and blaTEM. Molecular analysis using the polymerase chain reaction (PCR) method showed the genes blaCTX-M and blaTEM were detected in 11 isolates (91.67%), and blaSHV was found in 9 isolates (75%). The distribution pattern of the blaCTX-M, blaSHV and blaTEM resistance genes was present in 8 isolates (66.67%), with MIC values > 64 µg/mL. The presence of blaCTX-M, blaSHV, and blaTEM genes together in K. pneumoniae isolates represents a potential risk for resistance to other β lactam antibiotics.
The potential of short-chain fatty acids-producing probiotics as a treatment for liver disease: a systematic review Nur Azizah; Muhamad Rizqy Fadhillah; Nurul Gusti Khatimah; Rizky Clarinta Putri; Clara Riski Amanda; Fadilah, Fadilah; Oswari, Hanifah; Sjatha, Fithriyah
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 3 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i3.20181

Abstract

Recent insights reveal that liver diseases influence not only hepatic function but also disrupt gut microbial balance through the gut–liver axis. The gut–liver axis establishes a bidirectional relationship between the intestines and the liver, allowing microbial by-products such as short-chain fatty acids (SCFAs) to influence liver function and health. Short-chain fatty acids are known to maintain intestinal epithelial integrity, reduce inflammation, and support liver function. Probiotic bacteria including Lactobacillus, Bifidobacterium, and Clostridium, are natural SCFA producers and may offer therapeutic potential for liver disease by targeting the gut-liver axis. This systematic review was conducted using the PRISMA 2020 methodology to identify and evaluate preclinical studies examining the impact of SCFA-producing probiotics on liver disease. We searched PubMed, Scopus, and Google Scholar from August to October 2023, using predefined inclusion criteria based on the PICO framework. The SYRCLE risk of bias tool was employed to evaluate potential biases. A total of 14 animal studies fulfilled the inclusion criteria and were incorporated into the final analysis. The included studies demonstrated that SCFA-producing probiotics improved liver function by reducing serum liver enzymes (ALT, AST), increasing tight junction proteins (occluding, ZO-1), modulating pro-inflammatory cytokines (IL-1β, IL-6, TNF-α,), and improving lipid metabolism. These outcomes were mediated by increases in SCFA levels and improved gut barrier integrity in models of NAFLD, ALD, NASH, and autoimmune hepatitis. These findings support the promising potential of SCFA-producing probiotics as adjunctive therapies for liver disease through modulation of the microbiota-gut-liver axis. Yet, continued research is needed to determine strain-specific efficacy, optimal dosage, long-term safety, and clinical applicability. Future research should also explore personalized probiotic strategies and the integration of probiotic therapy into standard liver disease management.
Sampling of Sars-Cov-2 Covid-19 an Air Using the Impingement Method with Real-Time PCR as Detection Examination Tjampakasari, Conny Riana; Yasmon, Andi; Rosa, Yulia; Sjatha, Fithriyah
East Asian Journal of Multidisciplinary Research Vol. 3 No. 3 (2024): March 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/eajmr.v3i3.8651

Abstract

Examination of airborne microbial for SARS-CoV-2, Coronavirus Disease 2019 (Covid-19) has not been widely developed. Whether this virus is airborne is still an unanswered question. This preliminary study was to evaluate the presence of the Covid-19 in the air. The study was conducted on 4 rooms, which are laboratory rooms used for Covid-19 activities. The impingement which is an active method used for air sampling, while the detection was carried out through a molecular approach using RT real-time  PCR. The overall Covid-19 virus was not found in the room being examined. Four rooms gave CT values above the positive control, that is administration room (CT 30.44), BSL room (CT 30.23), BSC (CT 30.69 ) and waiting room (CT 35.61). No found SARS-CoV-2 Covid-19 in air using the impingement method with real-time PCR as detection examination in the room being examination.  Preliminary study shown SARS-CoV-2 Covid-19 can not transmitted through the air. Research needs to be done with more sampling as continuation of this study.
Co-Authors . Andriansjah Andi Yasmon Anis Karuniawati Beti Ernawati Dewi Clara Riski Amanda Engel, Yulia Fadilah Fadilah, Fadilah Hanifah Oswari Mardhiyah, Azzahrah Khairunnisa Marintan, Marchia Marthalena Mifa Nurfadilah Muhamad Rizqy Fadhillah Nur Azizah Nurani, Dita Arifa Nurul Gusti Khatimah OKTIVIA CHANDRA MUSTIKA Purnomosidi, Muhammad Abhi Rida Tiffarent Rizky Clarinta Putri Rosa, Yulia Rosdiana Irawati Sariman Sariman Supardi, Lulut Azmi Tjahjani Mirawati Sudiro Tjampakasari, Conny Riana Windi Muziasari Yulia Rosa Saharman Yuni Krisyuningsih Krisnandi, Yuni Krisyuningsih