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All Journal HAYATI Journal of Biosciences Indonesian Journal of Biotechnology Jurnal Mikologi Indonesia AL-HAYAT: Journal of Biology and Applied Biology Journal of Biology, Environment, and Edu-Tourism
Taufik, Intan
Sekolah Ilmu Dan Teknologi Hayati, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Produksi Konsentrat Pakan Ruminansia dari Kulit Kopi dan Dedak yang Difermentasi dengan Jamur Tiram Putih (Pleurotus ostreatus) Muhammad Yusuf Abduh; Lina Oktaviani; Intan Taufik
Jurnal Mikologi Indonesia Vol 3, No 1 (2019): Juni 2019
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.05 KB) | DOI: 10.46638/jmi.v3i1.50

Abstract

Penelitian yang dilakukan bertujuan untuk menentukan penambahan dedak yang optimal bagi remediasi limbah kulit kopi oleh jamur tiram putih (Pleurotus ostreatus) untuk menghasilkan sumber pakan ruminansia. Media kulit kopi diberi penambahan dedak dengan variasi persentase 0%, 25%, 50%, dan 75% dari total keseluruhan berat kering substrat. Media ditambahkan Ca(OH)2 sebanyak 1-2 gram dan air sebanyak 130 ml untuk mencapai pH media sebesar 6-7 dan kelembapan 55%-60%. Media dimasukkan ke dalam botol kultur dan disterilisasi dengan autoklaf pada suhu 121°C dan tekanan 1,5 atm. Bibit jamur tiram pada media jagung sebanyak 10 gram dikultivasi pada setiap variasi substrat. Kondisi inkubasi diatur pada suhu 23°C-25°C dalam keadaan gelap. Panjang miselium jamur tiram diukur setiap 5 hari. Kadar kafein, tanin, protein, lemak, dan abu diukur setiap 20 hari. Hasil penelitian menunjukkan media campuran kulit kopi dan dedak dengan persentase 25% kulit kopi dan 75% dedak dapat mengoptimalkan degradasi senyawa beracun pada kulit kopi dan meningkatkan kandungan protein miselium jamur. Kultivasi jamur tiram putih pada kombinasi media tersebut dapat mengurangi kadar kafein sebesar 7,3% dan tanin sebesar 79%, serta meningkatkan kadar protein sebesar 8%. Pemanfaatan kombinasi limbah ini dapat mengurangi polutan dan menghasilkan produk sumber pakan ruminansia yang sesuai dengan Standar Nasional Indonesia.
sgRNA design and in vitro nucleolytic analysis of the Cas9‐RNP complex for transgene‐free genome editing of the eIF4E1 gene from Capsicum an‐ nuum L. Josefanny Tham; Alfred Patisenah; Tommy Octavianus Soetrisno Tjia; Santiago Signorelli; Intan Taufik; Karlia Meitha
Indonesian Journal of Biotechnology Vol 28, No 4 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.86778

Abstract

Chili (Capsicum annuum L.) is a highly valued vegetable, renowned for its unique taste and aroma. However, chili production faces challenges in meeting the high demand due to infections caused by pathogens such as ChiVMV (potyvirus). Previous studies have suggested that chili eIF4E1 plays a crucial role in potyvirus gene transcription. Therefore, this study explores the potential of CRISPR‐Cas9‐based genome editing to enhance chili resistance by introducing premature stop codons or truncated proteins. Two sgRNAs were designed, targeting the first and second intron of the eIF4E1 gene. The production of Cas9 protein was assessed with varying IPTG concentrations in Escherichia coli BL21(DE3), carrying 4xNLS‐pMJ915v2‐sfGFP plasmid with a TEV protease cut‐site at the N terminal. The findings indicate that the optimal IPTG concentration is 500 µM. Purification using an IMAC column confirmed the presence of Cas9 in the initial 2 mL of the eluted fractions, as indicated by numerous background proteins. Nevertheless, successful formation of Cas9‐RNP complexes was achieved for both sgRNAs. The nucleolytic activity of Tag‐Cas9 (carrying the MBP‐tag) and Cas9 was confirmed through in vitro endonuclease activity assays. The next step involved transfecting chili protoplasts with these RNP complexes to edit the chili eIF4E1 gene.
Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21 Taufik, Intan; Fanany, Rizal; Manjaswari, Agika; Pratama, Fenryco
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.155-163

Abstract

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (PCR) method. The growing demand for Bst DNA polymerase highlights the need for cost-effective in-house production, as a commercial option is economically challenging. For that purpose, this study aims to construct and optimize the expression of Bst DNA polymerase from Geobacillus stearothermophilus in Escherichia coli. The expression constructs pET16b.BstHF vector was constructed using Gibson Assembly and expressed in E. coli BL21 (DE3). Optimal expression was achieved with 1 mM IPTG, induction at OD600 0.8 and 6-hour induction time. The purified enzyme was achieved with a protein yield of 2,175 mg/L culture and demonstrated effective polymerase activity for LAMP.
Starter Culture Modulates Microbial Diversity During Wine-Coffee Fermentation: A DGGE-Based Molecular Study Nasir, Fitri Handayani; Astuti, Dea Indriani; Taufik, Intan; Fitriagustiani; Ikramullah, Muh. Chaeril; Putri, Nurul Febriani; Pasmawati
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.28269

Abstract

Wine-flavored coffee is a unique post-harvest product characterized by fruity, acidic, and winey notes, highly favored by Indonesian consumers and possessing strong potential in the global market. However, maintaining consistency in flavor and quality remains a challenge due to the variability of natural fermentation. This study aimed to evaluate the impact of yeast and bacterial starter culture inoculation on microbial community dynamics during wine-coffee fermentation, using a molecular approach based on PCR-DGGE. DGGE analysis revealed that natural fermentation without inoculation involved diverse populations of bacteria, yeasts, and filamentous fungi, with eukaryotic microbes dominating from the early stages. Sequencing identified prevalent yeast genera including Pichia, Torulaspora, Hanseniaspora, Saccharomyces, and Candida, as well as Aspergillus among filamentous fungi. Bacterial communities were dominated by lactic acid bacteria and members of Lactobacillus, Klebsiella, Enterobacter, Pantoea, and Bacillus. In contrast, controlled fermentation with inoculated starter cultures (Pichia kudriavsevii and Klebsiella sp.) showed a more stable microbial profile throughout the process. Shannon-Wiener diversity indices demonstrated a significant difference (p = 0.05) between natural and inoculated fermentations, with species dominance observed in the latter. Cluster analysis confirmed that starter culture inoculation significantly influenced microbial succession and community structure. These findings highlight the importance of controlled fermentation using selected microbial starters to ensure consistent microbial ecology, which in turn contributes to reproducible quality in wine-flavored coffee. The molecular profiling approach provides valuable insights for improving fermentation practices and developing reliable starter culture formulations tailored to enhance flavor consistency and product quality.
Development of community-based coffee agrotourism in West Nusa Tenggara province Aida Muspiah; Intan Taufik; Amrullah
Journal of Biology, Environment, and Edu-Tourism Vol. 1 No. 3 (2025): December
Publisher : Yayasan Siti Widhatul Faeha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65622/jbee.v1i3.166

Abstract

Sustainable rural development requires strategies that optimise local resources while maintaining environmental and socio-cultural sustainability. The purpose of this study is to analyze the development of community-based coffee agrotourism in East Lombok Regency, West Nusa Tenggara Province, as a strategy to strengthen sustainable rural economic development. The research method uses a qualitative descriptive approach from secondary data to analyze the biophysical potential of local coffee-based agroecosystems, the institutional readiness of farmer groups and regional organizations, and existing collaboration mechanisms between government, communities, academics, and private actors. The results indicate that East Lombok has the potential to be developed into coffee agrotourism based on its natural and socio-cultural conditions, agricultural climate, farming communities, and local traditions. Obstacles faced include tourism infrastructure, community management capacity, and weak coordination between institutions. The recommended model to overcome the difficulties of agrotourism development is Community-Based Tourism (CBT), which integrates economic, socio-cultural, and ecological dimensions. In addition, cross-sector collaboration (quadruple helix) is needed to ensure a stable distribution of economic and environmental benefits. Therefore, the results of this study provide a practical basis for planning and policy-making related to the development of inclusive and sustainable agrotourism.
Co-Authors Abduh, Muhammad Yusuf Aida Muspiah Alfred Patisenah Amrullah Dea Indriani Astuti Fanany, Rizal FENRYCO PRATAMA Fitriagustiani Ikramullah, Muh. Chaeril Josefanny Tham Karlia Meitha Lina Oktaviani Manjaswari, Agika Nasir, Fitri Handayani Pasmawati Putri, Nurul Febriani Santiago Signorelli Tommy Octavianus Soetrisno Tjia