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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Biomedical Journal of Indonesia Molecular and Cellular Biomedical Sciences (MCBS) Eksakta : Berkala Ilmiah Bidang MIPA Pharmaceutical Journal of Indonesia Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang Proceedings of the International Conference on Nursing and Health Sciences Jurnal Kesehatan Komunitas Indonesia Journal of Clinical Microbiology and Infectious Diseases Indonesian Journal of Biomedicine and Clinical Sciences
Ibnu Agus Ariyanto
Department Of Microbiology, Faculty Of Medicine, Universitas Indonesia, Jakarta, Indonesia
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SARS-CoV-2 multi-epitope subunit vaccine proof-of-concept derived from the in silico study with protein expression in E. coli BL21 Muhayya, Syarifah Raisha; Agus Ariyanto, Ibnu; Widianingtyas, Silvia; Subiantistha, Tanaya; Bela, Budiman
Pharmaceutical Journal of Indonesia Vol. 9 No. 2 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2024.009.02.1

Abstract

The protein subunit vaccine is the most considerably developed SARS-Cov-2 vaccine, according to the WHO vaccine tracker in 2023. The acceleration of vaccine development in 2 years of eradicating the COVID-19 pandemic is attainable due to the role of bioinformatics. The objective of this paper is to evaluate strategies for developing multi-epitope SARS-Cov-2 recombinant vaccines with high protein expression in silico. The study was conducted by analyzing SARS-Cov-2 epitopes using immunoinformatics tools provided by IEDB, codon optimization, rare codon analysis, plasmid design, and ribosomal binding site (RBS) analysis were analyzed using RNA structure 6.4, gene cloning by E. coli DH5α and protein expression by E. coli BL21. Each epitope peptide candidate was linked to a flexible linker sequence (GGGGS). GelAnalyzer 19.1 was utilized to determine the protein band of SDS-PAGE. The immunoinformatics study obtained multi-epitope of the recombinant SARS-COV-2 vaccine with a total of 7 epitopes for HLA-I allele candidates and 4 for HLA-II. It is demonstrated that the candidate vaccine protein was successfully cloned in E. coli DH5α and expressed in E. coli BL21.
Co-Authors Alana Arumsari Amin Soebandrio Andi Mahendra Andi Yasmon Anindya, Isti Ardaning Nuriliani Arif Luthfi Nurul Huda Ariyani Noviantari Bebby Shelby Budiman Bela Chairinda Dachwan Chesya Ejoli Clara Imaniar Dariatul Janah Delly Chipta Lestari Elfrida Ratnawati Erie Yuwita Sari Heri Wibowo Imaniar, Clara Lukman Edwar Mardiastuti Mardiastuti Maya Ulfah Mei Ria Santi Mochamad Helmi Aziz Muhayya, Syarifah Raisha Nastiti Wijayanti Nastiti Wijayanti Ni Wayan Erly Sintya Dewi Patricia Price Puspa Andhia Kemalasari Ratih Rinendyaputri Ratna Sitompul Rifka Kamila Rini Sekartini Rivia Gina Rahmawaty Sari, Novika Purnama Selita Agnes Tanudjaja Selvi Nafisa Shahab Silvia Lee Subiantistha, Tanaya Tjhin Wiguna Utami, Diah Dwi Widianingtyas, Silvia Wisnu Tafroji Yeva Rosana Yundari, Yundari