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All Journal Jurnal Ilmu Dasar Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) International Journal of Natural Science and Engineering BIOSFER : Jurnal Biologi dan Pendidikan Biologi Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Abdi Insani Journal of Tropical Ethnobiology Soeropati: Journal of Community Service International Islamic Medical Journal Rumphius Pattimura Biological Journal Proceeding of International Conference on Biology Education, Natural Science, and Technology
Beandrade, Maya Uzia
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Journal : BIOSFER : Jurnal Biologi dan Pendidikan Biologi

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Bioprospecting Test of Piper betle Leaf Essential Oil Against Staphylococcus Aureus and Escherichia Coli-Antibiotic Resistant Anindita, Reza; Beandrade, Maya Uzia; Putri, Intan Kurnia
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol. 9 No. 2 (2024): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/biosfer.v9i2.19303

Abstract

One of the efforts to overcome antibiotic resistance is the bioprospection of natural materials that have the potential to be antibacterial. One of the results of distilling natural materials with antibacterial potential is betel leaf essential oil (Piper betle). This study aimed to determine the effect of essential oils on Staphylococcus aureus and Escherichia coli-resistant antibiotics. The design of this study is experimental, with independent variables in the form of essential oils with concentrations of 5%, 10%, 15%, 20%, 25%, and 100%. In contrast, the dependent variable is the diameter of the growth inhibition zone of S. aureus and E.coli bacteria. Essential oil extraction using steam distillation and chemical characterization by GC-MS test. Antibacterial testing uses the Kirb-Bauer method. GC-MS test results were dominant eugenol (3.50%), caryophyllene (3.79%), and Germacrene D (1.19%). The application of essential oils was able to inhibit the growth of S. aureus with inhibition zone diameters of 10.5 mm (moderate), 10.6 mm (moderate), 10.8 mm (moderate), 12.3 mm (strong), 14 mm (strong), and 30.2 mm (very strong), while E. coli was 5.7 mm (moderate), 7.5 mm (moderate), 10.5 mm (moderate), 12 mm (strong), and 30.03 mm (very strong). The results of the ANOVA one-way test showed a value of 11762.7, while the F table was 3.48 (Fcal > Table). This study concludes that administering essential oils with concentrations of 5%, 10%, 15%, 20%, 25%, and 100% can significantly inhibit the growth of S. aureus and E.coli bacteria.
Co-Authors Afrinia Ekasari Amelia, Ria Arianti, Anisa Rachmita Auliya Rahmawati, Auliya Futihat, Chilyati Eky Haediningrum, Syahla Harahap, Nofria Rizki Amalia Harahap, Salwa Zhafarina Hasmar, Wahyu Nuraini Inggraini, Maulin Melania Perwitasari, Melania Nathalia, Dede Dwi Pratama, Gita Cahya Putri, Intan Kurnia Rahmadi, Chandra Setyodewi, Elisabeth Tahapary, Manuela Esterlita Wahyu, Ega Nuraini