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Uji Nanoemulsi Ecoenzym Terhadap Bakteri Staphylococcus aureus anindita, Reza; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia; Sari, Afrinia Eka; Setyodewi, Elisabeth
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.553

Abstract

The discovery of eco enzyme as a fermented liquid from organic vegetable and fruit waste has been used as an organic plant fertilizer, disinfectant, hand sanitizer, antiseptic soap, and domestic waste degradation agent. However, trials of eco enzymes as raw materials for health preparations have not yet been carried out. Seeing this, trial research is needed on the use of ecoenzyme nanoemulsion against Staphylococcus aureus bacteria. This research aims to determine the antibacterial ability of ecoenzyme nanoemulsion against S. aureus bacteria. The design of this research is experimental. The samples used were ecoenzyme nanoemulsions with Formula 1 (F1) 20%, Formula 2 (F2) 25%, and Formula 3 (F3) 30%. This research includes making ecoenzyme, making ecoenzyme nanoemulsion, testing ecoenzyme nanoparticles, and testing ecoenezyme nanoemulsion against S. aureus bacteria using the Kirby-Baeur method. The results showed that the sizes of ecoenzyme nanoparticles in F1 20%, F2 25%, and F3 30% were 38.9 nm, 65.07 nm, and 146.2 nm, respectively. The results of the antibacterial ability test of nanoemulsion eco enzyme F1 20%, F2 25%, and F3 30% against S. aureus produced an inhibitory zone 4.3 mm (resistant), 4.3 mm (resistant), and 6.7 mm (resistant). This research concludes that the eco enzyme nanoemulsion is not effective in inhibiting the growth of S. aureus bacteria.
Monitoring The Correlation of Climatics to The Airborne Bacteria at The Manggarai Station, South Jakarta, Indonesia Anindita, Reza; Rahmawati, Auliya; Inggraini, Maulin; Perwitasari, Melania; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The highest of microorganisms suspended in the air were bacteria with a percentage of 80.8%. If the intensity of bacterial exposure occurs in large quantities, it will accumulate in the respiratory tract which has the potential to trigger allergic reactions and respiratory infections. Given the problems and impacts caused by air microorganisms on public health, it is necessary to monitor the distribution of air bacteria. The purpose of this study was to determine the relationship between temperature and humidity with the number of bacteria in the Manggarai station area. Type of research is correlation. The sampling location is Manggarai Station, East Jakarta, Indonesia. The 10 sampling points in this study include the station hall, motorbike parking, prayer rooms, train platform 1-2, train platform 2-3, train platform 3-4, and train platform 5, toilet, and underpass. The results of this study included the highest number of bacteria found in the station hall 331 colonies, the highest percentage of bacterial morphology is monobacilli was 78%, the distribution of gram positive was more than gram negative with spore bacteria being more dominant than non spore. The correlation coefficient between temperature and number of bacterial colonies (0.134) and humidity with number of bacterial colonies (0.380) showed weak positive correlation, while the correlation coefficient for light intensity with the number of bacterial colonies -0.140 (very weak negative).
SIMULASI PEMBUATAN SABUN CAIR EKSTRAK DAUN KEMANGI DAN BALSAM EUCALYPTUS OIL BAGI SISWA/I SMA/K DI KOTA BEKASI Anindita, Reza; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia; Perwitasari, Melania; Harahap, Nofria Rizki Amalia
Jurnal Abdi Insani Vol 11 No 1 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i1.1439

Abstract

Discourse on the utilization of medicinal plants, which have the potential to be made into liquid soap and balsam, is very much needed for SMA and SMK students as knowledge in doing entrepreneurship in developing pharmaceutical products. Therefore, community service activities (PKM) are needed to provide knowledge and skills regarding the manufacture of liquid soap from basil and balsam extracts from Eucalyptus oil. The purpose of this PKM activity is to provide knowledge, understanding, and skills regarding the procedures for making liquid soap from extracts of basil and balsam leaves from Eucalyptus oil. This PKM activity includes the planning stages, such as making socialization flyers, materials, and videos on making soap and balm from natural ingredients. The implementation stage of providing materials and simulations for SMA/K children in Bekasi City at the STIKes Mitra Keluarga Pharmaceuticals Laboratory, Bekasi. The Evaluation Stage is in the form of taking a response score on the use of liquid soap and balsam by the PKM participants. The results of this PKM showed that there were 26 SMA/SMK students in attendance in Bekasi City, with 84.61% of SMK students dominating the participants. The preferred product was Balsam Eucalyptus oil, especially in terms of aroma, texture, and warmth. This PKM concludes that participants have a new understanding of the use of basil leaves and Eucalyptus oil for making liquid soap and balsam. The product that the participants liked was balsam Eucalyptus oil compared to basil leaf extract liquid soap.
MICROSCOPE ON THE USAGE TRAINING AS A FORM FOR ACHIEVING SDGS FOR HIGH SCHOOL STUDENTS IN INDONESIA Anindita, Reza; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia
As-Sidanah Vol 7 No 2 (2025): OKTOBER
Publisher : LP2M Universitas Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35316/assidanah.v7i2.435-454

Abstract

One of the instruments that is often not available for cell observation skills in high schools in Bekasi City is a microscope. Therefore, it is necessary to carry out community service activities (CSA) in the form of training in the use and maintenance of microscopes, making and observing cell slides, and cell observation documentation techniques for high school students in Bekasi City. This CSA method, based on Asset-Based Community Development (ABCD) and Participatory Action Research (PAR), involves planning integrated CSA programs offered to several high schools in Bekasi City. The implementation stage was carried out in 6 meetings (each meeting started from 08.00-16.00) by providing a pre-test, delivering materials, conducting a post-test, practicing microscope use, making and observing cell slides, and documenting cell slide techniques. The evaluation stage was carried out by processing qualitative and quantitative data. The results of this CSA include 30 students: 19 from Al-Muhtadin High School and 11 from Annida Al-Islamy, MA. Statistical analysis using paired t-tests produced a sig value. 0.01<0.05 or CS activities can significantly increase the average value of knowledge about microscopes, while the average value of the skills test is 76.92. This CSA concludes that providing microscope materials and training as a CS activity package can enhance the knowledge and skills of 19 Al-Muhtadin High School students and 11 Annida Al-Islamy MA students in observing cells using a light microscope.
Chemical Analysis, Physical Stability, and Antibacterial Activity of Nanoemulgel Hand Sanitizer Formulated with Citrus aurantifolia Essential Oil and Herbal Emollients Putri, Intan Kurnia; Beandrade, Maya Uzia; Anindita, Reza
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1305-1314

Abstract

One of the most purchased hand sanitizer products by the public since the COVID-19 pandemic is hand sanitizer. However, alcohol-based hand sanitizer products often cause toxic effects and bacterial resistance. Therefore, alternative efforts are needed to replace antibacterial raw materials instead of alcohol, namely lime peel essential oils (Citrus aurantifolia) with Aloe vera and Calendula officinalis emollients.  This study aims to evaluate the physicochemistry, physical stability, and antibacterial affectiveness of the a nanoemulgel handsanitizer formula composed of active lime peel essential oils supplemented with Aloe vera and Calendula officinalis emollients. The design of this research is an experiment. Research treatment: formula (F) hand sanitizer nanoemulgel F1 2%, F2 4%, and F6 6%. Essential oil component test using GC-MS method, SNEEDS physical stability includes centrifugation test, freeze-thawing, transmittance, particle size, polydispersity index (PI), potential zeta, physical stability of nanoemulgel hand sanitizer, including organoleptic, homogeneity, pH, and viscosity test, antibacterial efficacy test using hand swabbing method. The results: GC-MS analysis of the components of lime peel essential oils: alpha-pinene (8.87%), linalool (0.58%), and L-alpha-terpineol (8.98%). All SNEEDS formulas and nanoemulgels were stable during 6 weeks of storage. Formulas F2 4% and F3 6% reduced the percentage (%) of hand bacterial colonies to 74% and 75%. The results of the one-way ANOVA showed a significant decrease (p < 0.05) in the number of bacterial colonies after using nanoemulgel hand sanitizer, especially in the F2 and F3 formulas. The conclusion is that Formula 3 (F3 6%) hand sanitizer is recommended as the best candidate for the development of a nanoemulgel hand sanitizer based on lime essential oil, as it offers the most optimal combination of physical stability, chemical stability, and biological effectiveness.
Antioxidant Activity Interaction of Rosella (Hibiscus sabdariffa L.) and Soursop Leaves (Annona muricata L.) Brew Combination Using the FRAP (Ferric Reducing Antioxidant Power) Method Apriyani, Zupita; Perwitasari, Melania; Beandrade, Maya Uzia; Nathalia, Dede Dwi
Journal of Fundamental and Applied Pharmaceutical Science Vol. 6 No. 2 (2026): February
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jfaps.v6i2.23776

Abstract

Free radical compounds can damage human body cells, triggering degenerative diseases. The prevention effort is to consume natural ingredients such as rosella and soursop leaves, which have the potential as antioxidants. This study is to determine the antioxidant activity of rosella and soursop leaf brew and their combination interaction. This research uses experimental quantitative methods. The samples used were F1 (rosella), F2 (soursop leaves) and their combination with concentration variations F3 (50:50), F4 (75:25), and F5 (25:75). Antioxidant activity values were determined by the FRAP method and analyzed using One-way ANOVA. The antioxidant activity value of rosella, soursop leaves and their combinations F3, F4 and F5 are 69.222 µg/mL; 133.701 µg/mL; 104.049 µg/mL; 57.382 µg/mL; and 115.438 µg/mL. Analysis: One-way ANOVA test of rosella and soursop leaf brew with several concentration variations has significant differences, sig. 0.000 <0.05 on the value of antioxidant activity. The antioxidant activity of the soursop leaf brew was higher than rosella brew. The combination interaction was categorized as antagonistic, with a % difference <0.