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All Journal Public Health of Indonesia Jurnal Mandala Pengabdian Masyarakat Jurnal Kesehatan Masyarakat
Damiti, Sukmawati Ahmad
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Education Public Health Social Sciences

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Edukasi Pemanfaatan Bahan Pangan Lokal Kelakai pada Ibu Balita Dalam Upaya Pencegahan Stunting: Edukasi Pemanfaatan Bahan Pangan Lokal Kelakai pada Ibu Balita Dalam Upaya Pencegahan Stunting Mashar, Harlyanti Muthma'innah; Damiti, Sukmawati Ahmad; Dali, Dali
Jurnal Mandala Pengabdian Masyarakat Vol. 5 No. 2 (2024): Jurnal Mandala Pengabdian Masyarakat
Publisher : Progran Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpm.v5i2.445

Abstract

Kelakai (Stenochlaena palutris) merupakan salah satu pangan lokal di Kalimantan Tengah yang kaya akan zat gizi dan sangat lazim dikonsumsi oleh masyarakat serta sangat mudah diperoleh disekitar rumah masyarakat. Pangan lokal yang mudah diperoleh di lingkungan sekitar masyarakat dapat membantu untuk mengatasi kerawanan pangan dan kekurangan gizi sehingga dapat dimanfaatkan sebagai upaya untuk penanganan stunting, khususnya di Kalimantan Tengah. Kegiatan ini bertujuan untuk meningkatkan pengetahuan ibu balita di Wilayah Kerja Puskesmas Kereng Bangkirai tentang pemanfaatan bahan pangan lokal kelakai dalam pencegahan stunting. Metode pelaksanaan kegiatan yaitu dengan memberi edukasi untuk mencapai peningkatan pengetahuan sasaran dengan peserta sebanyak 15 orang. Evaluasi ketercapaian legiatan yaitu dengan mengukur pengetahuan ibu balita sebelum dan sesudah diberi edukasi. Indikator keberhasilan jika lebih dari 75% peserta kegiatan telah memahami dengan baik materi yang disampaikan. Hasil yang diperoleh kemudian dianalisis secara statistik. Hasil kegiatan menunjukkan bahwa terjadi peningkatan pengetahuan peserta menjadi sebagian besar kategori baik setelah diberi penyuluhan (86,67%). Hasil ini menunjukkan keberhasilan pelaksanaan kegiatan dengan indikator peserta memperoleh kategori pengetahuan baik >75%. Hasil menunjukkan terdapat perbedaan signifikan terhadap pengetahuan dengan nilai p=0,012 (<0,05).
The Cytotoxicity of Endophytic Fungi Isolated from Kelakai (Stenochlaena palustris) Against MCF-7 and Vero Cells Dali; Mashar, Harlyanti Muthma’innah; Damiti, Sukmawati Ahmad; Ysrafil, Ysrafil
Public Health of Indonesia Vol. 11 No. 3 (2025): July - September
Publisher : YCAB Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36685/phi.v11i3.1002

Abstract

Background: The high prevalence of cancer has a direct impact on the quality of life and nutritional status of sufferers, mainly due to decreased appetite, metabolic disorders, and side effects of conventional therapies such as surgery, mastectomy, chemotherapy, hormonal therapy, or radiation therapy. These therapies, although aimed at inhibiting the growth and metastasis of cancer cells, often cause severe side effects that worsen the patient's condition. Therefore, it is important to explore alternative treatments that are effective and have minimal side effects. In this regard, biological sources, such as endophytic fungi kelakai, contain various active secondary metabolites that can inhibit cancer cell proliferation so that they can be part of a more public health-friendly supportive therapy approach.Objective: This study aims to prove the cytotoxic activity of the extract of endophytic fungi in kelakai against MCF-7 and Vero cells.Methods: Isolation, purification, and extraction process of secondary metabolites from the endophytic fungi isolates were carried out in the same manner as our previous research. The ethyl acetate extract of kelakai was obtained by extractive fermentation from pure isolates of the endophytic fungi in kelakai. Extraction was carried out by the fractionation method using the ethyl acetate solvent at a ratio of 1:1. Identification of chemical contents, including tannins, alkaloids, flavonoids, saponins, and steroids/terpenoids, was conducted qualitatively. Cytotoxic activity was tested by the MTT method.Results: From the isolate extraction, 2.4 g of ethyl acetate extract was produced. The identification of chemical contents showed positive results, meaning that the extract did contain tannins, flavonoids, alkaloids, steroids, and saponins. The extract cytotoxicity test against MCF-7 and Vero cells yielded IC50 values of 517.3015 μg/ml and 1,074.9152 μg/ml, respectively. Conclusion: Based on these results, the extract of kelakai was not toxic to Vero cells, but it was toxic to MCF-7 cells. These findings suggest that the kelakai can be the basis for exploring local plants as a source of natural medicine while supporting the conservation of endemic plants to provide safer alternative therapies and improve public health. Keywords: Stenochlaena palustris; Endophytic Fungi; MTT; MCF-7; Vero cell
Mineral Content and Antioxidant Capacity of Cookies Formulated with Spinach and Pangas Catfish Mashar, Harlyanti Muthma'innah; Mahalia, Lamia Diang; Damiti, Sukmawati Ahmad
Jurnal Kesehatan Masyarakat Vol. 21 No. 1 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/qh9vdp63

Abstract

Spinach is a leafy green vegetable that contains various nutrients and antioxidant compounds. Meanwhile, Pangas catfish contains calcium and minerals that are very good for health. Spinach and Pangas catfish can be utilized as raw ingredients to manufacture functional food products in the shape of cookies, increasing the nutritional value and antioxidant content of the cookies to increase the fulfillment of food and public health needs. This research was conducted from February to July 2024. Cookies were prepared with three variations of spinach flour to Pangas catfish flour ratio designated as F1 (45:15), F2 (30:30), and F3 (15:45). Analysis of mineral, vitamin C, TPC, TFC, and antioxidant activity was performed on each cookie formula. The study results showed that the mineral content in terms of potassium and calcium was in the range of 323.538 to 671.186 mg/100g and 44.307 to 93.006 mg/100g, respectively. Meanwhile, the bioactive compounds in the form of vitamin C, TPC, and TFC were in the range of 9.92 to 20.02 mg/100g, 16.74 to 24.97 mg GAE/g, and 32.55 to 110.84 mg QE/g, respectively, and the antioxidant activity was 8.11-17.84%. The cookies prepared using the formula F1 showed significant content of minerals, i.e., potassium and calcium, vitamin C, TPC, TFC, and significant antioxidant activity. These cookies have the potential to be developed as a functional food product that is rich in nutrients and antioxidants to better meet the nutritional and public health needs.
Co-Authors Dali Dali, Dali Harlyanti Muthma'innah Mashar Lamia Diang Mahalia, Lamia Diang Mashar, Harlyanti Muthma’innah Ysrafil, Ysrafil