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All Journal Indonesian Journal of Chemistry Narra J Jurnal Farmasi Etam SAINSTECH: Jurnal Penelitian dan Pengkajian Sains dan Teknologi Jurnal Ilmiah Kesehatan Jurnal Sains dan Kesehatan Journal of Natural Product for Degenerative Diseases
Ana Yulyana
Program Magister Ilmu Kefarmasian, Fakultas Farmasi, Universitas Pancasila, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Mechanical Engineering Medicine & Pharmacology Nursing Public Health

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Analisis Fitokimia dan Aktivitas Antibakteri Infusa Daun Sempur (Dillenia suffruticosa (Griff.) Martelli) Terhadap Shigella dysentriae dan Staphylococcus aureus Vilya Syafriana; Nuke Paramitha Dewanti; Ana Yulyana
Jurnal Farmasi Etam (JFE) Vol 1 No 2 (2021): Desember
Publisher : Unit Penelitian dan Pengabdian Kepada Masyarakat (UPPM) STIKES Dirgahayu Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.993 KB) | DOI: 10.52841/jfe.v1i2.207

Abstract

Sempur (Dillenia suffruticosa (Griff.) Martelli) merupakan tanaman hutan tropis yang dapat dijumpai di hutan Sumatra dan Kalimantan Indonesia. Secara empiris, daun dari tanaman ini dimanfaatkan untuk berbagai pengobatan seperti penyembuh luka, antidiabetes, dan antidiare. Pemanfaatan tanaman ini sebagai antidiare adalah dengan meminum air rebusannya. Tujuan penelitian ini untuk mengetahui aktivitas infusa daun sempur terhadap bakteri penyebab diare seperti Shigella dysentriae dan Staphylococcus aureus secara in vitro. Infusa dibuat dengan cara pemanasan selama 15 menit pada suhu 90°C menggunakan aquadest sebagai pelarut. Infusa yang diperoleh dianalisis kandungan fitokimianya secara kualitatif meliputi uji alkaloid, flavonoid, tanin, saponin, triterpenoid, dan steroid. Infusa selanjutnya diuji aktivitas antibakterinya terhadap S. dysentriae dan S. aureus menggunakan metode difusi cakram pada konsentrasi 5%, 10%, 20%, dan 40%. Hasil analisis fitokimia menunjukkan bahwa infusa daun sempur mengandung alkaloid, flavonoid, tanin, dan saponin. Hasil uji aktivitas antibakteri menunjukkan bahwa infusa daun sempur pada setiap konsentrasi tidak mampu menghambat pertumbuhan kedua bakteri uji. Hal ini menggambarkan bahwa hasil uji in vitro tidak sejalan dengan kondisi empiris, akan tetapi hasil ini belum bisa dijadikan patokan bahwa air rebusan daun sempur tidak mampu mengobati diare. Perlu dilakukan penelitian lanjutan menggunakan konsentrasi yang lebih tinggi hingga 100% untuk memastikan kemampuan infusa daun sempur sebagai antidiare.
Formulasi Antioksidan Minyak Daun Lemo (Litsea cubeba Pers.) Dengan Oleum Olivarum Sebagai Pelarut Ana Yulyana; Satrio Satrio Ari Hutomo; Munawarohthus Sholikha
Jurnal Ilmiah Kesehatan Vol. 17 No. 1 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Muhammadiyah Pekajangan Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.48144/jiks.v17i1.1781

Abstract

Litsea cubeba, also known as lemo, is a plant rich in antioxidant compounds and has been used in various studies to explore its potential in protecting the skin from oxidative damage. Recent research indicates that Litsea cubeba leaf oil possesses significant antioxidant activity, measured using the DPPH method, demonstrating its capacity to counteract free radicals. Additionally, this oil also contains vitamin E, known for its antioxidant properties, with measurable levels found in the studies. The physical stability of the lemo leaf oil formulation has also been evaluated, showing no change in shape or smell after a 14-day cycling test, indicating good potential for long-term application. These results affirm that Litsea cubeba holds great promise as a raw material in skincare products, particularly as an antioxidant, which can help protect the skin from the negative effects of free radicals and oxidation processes.
Hubungan Antara Kepatuhan Penggunaan Obat Dan Keberhasilan Terapi Pasien Diabetes Mellitus Refdanita Refdanita; Lili Musnelina; Ana Yulyana; Ratna Hidayah Herawati; Nerry Miellana
Jurnal Ilmiah Kesehatan Vol. 17 No. 1 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Muhammadiyah Pekajangan Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.48144/jiks.v17i1.1784

Abstract

Diabetes Mellitus merupakan penyakit kronis yang tidak dapat disembuhkan,sehingga terapi diberikan secara terus menerus dengan tepat. Salah satu penentu keberhasil terapi adalah kepatuhan penggunaan obat. Tujuan penelitian ini adalah untuk mengetahui hubungan antara kepatuhan penggunaan obat dan keberhasilan terapi pada pasien DM. Penelitian ini merupakan jenis penelitian ini adalah diskriptif analitik dengan desain cross sectional dan pengambilan data menggunakan metode prospektif. Penelitian ini dilakukan pada 235 pasien diabetes mellitus tipe 2 rawat jalan yang teratur kontrol dan mendapatkan antidiabetik oral pada terapinya. Responden ditetapkan dengan teknik purposive sampling. Hubungan skor kepatuhan dengan penurunan kadar gula darah puasa dianalisis menggunakan chi-square. Ditemukan pada penelitian ini bahwa penyakit DM lebih banyak diderita oleh laki-laki dengan usia ≤ 65 tahun (57.4%).Terapi DM yang banyak diberikan adalah dalam bentuk kombinasi Metformin & Glimepiride (25,5%). Tingkat kepatuhan pasien berada pada tingkat tinggi (81,3%) dan tingkat rendah (18,7%).Dari analisis chi-square didapat nilai signifikasi 0.091> 0.05 maka, tidak terdapat hubungan bermakna antara kepatuhan penggunaan obat dengan keberhasilan terapi pada pasien DM
Karakterisasi Ekstrak Daun Cantigi (Vaccinium varingiaefolium Miq.) Ana Yulyana; Hendig Winarno; Kosasih Kosasih
Jurnal Sains dan Kesehatan Vol. 1 No. 5 (2016): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v1i5.50

Abstract

Cantigi (Vaccinium varingiaefolium Miq.) banyak tumbuh di sekitar kawah gunung berapi namun belum banyak informasi yang terkuak. Tujuan penelitian: Melakukan karakterisasi ekstrak daun Cantigi yang diekstraksi secara maserasi menggunakan pelarut n-heksan, etilasetat, dan etanol 95%; penapisan fitokimia ekstrak; uji aktivitas sitotoksik ekstrak; fraksinasi ekstrak dengan kromatografi kolom; uji sitotoksik fraksi; pembuatan profil GC/MS fraksi teraktif. Hasil penelitian: Hasil penapisan fitokimia menunjukkan positif untuk flavonoid, steroid, tannin, dan triterpenoid. Aktivitas sitotoksik pada sel leukemia L1210 (IC50) 12,67; 8,29; dan 10,42 µg/mL. Fraksinasi ekstrak teraktif etilasetat (Kromatografi kolom: Fase diam silika gel 60, fase gerak kloroform:metanol 10:1, 8:1, dan 4:1) diperoleh 4 fraksi. Aktivitas sitotoksik (IC50) Fraksi 1-4 adalah 3,62; 1,69; 2,98; dan 2,96 µg/mL. Karakterisasi fraksi F2 dengan GC/MS menunjukkan 15 senyawa. Simpulan: Aktivitas sitotoksik fraksi ekstrak etilasetat (F2) lebih tinggi dibandingkan non-fraksi ekstrak etilasetat dan ini belum pernah dilaporkan sebelumnya.
Synergistic mechanism of Phyllanthus emblica extract and tetracycline against multidrug-resistant Acinetobacter baumannii Nasution, Haris M.; Yulyana, Ana; Utama, Rezza F.; Bangar, Roy I.; Kaban, Vera E.; Daulay, Wardiyah; Astyka, Ririn; Lubis, Muhammad F.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1939

Abstract

The rising incidence of multidrug-resistant (MDR) Acinetobacter baumannii infections underscores the urgent need for novel antimicrobial strategies. The aim of this study was to investigate the synergistic effects between a polyphenol-rich extract from Phyllanthus emblica fruit and tetracycline against MDR A. baumannii strains. The extraction process was optimized using the Box-Behnken design approach to maximize the total phenolic content (TPC) of the P. emblica extract. Key variables, including ethanol concentration, extraction time, and solid-to-liquid ratio (w/v), were determined through single-factor experiments. The antimicrobial activity of the extract, both alone and in combination with tetracycline, was evaluated against A. baumannii. Mechanistic studies focusing on bacterial lysis and efflux pump inhibition were conducted to assess the extract's effects and its combined potential with tetracycline. The Box-Behnken design successfully optimized the extraction conditions, yielding the highest TPC at 68.92% ethanol concentration, 1.85 days of extraction time, and a 1:9.58 w/v ratio. The predicted and experimentally verified TPC values of the extract were 129.19 and 130.76±2.46 mg GAE/g samples, respectively, with no significant difference (p>0.05). The extract contained several phenolic compounds identified using liquid chromatography–high-resolution mass spectrometry (LC-HRMS). It exhibited antimicrobial activity against MDR Acinetobacter baumannii, either alone or in combination with tetracycline. The combination demonstrated a synergistic effect against MDR A. baumannii, with a fractional inhibitory concentration index (FICI) of 0.37. Moreover, the combination showed superior bacteriolytic effects against MDR A. baumannii cells, as evidenced by increased release of nucleic acid components and membrane destabilization, compared to the extract or tetracycline alone (p<0.0001 for all comparisons). Additionally, the combination significantly enhanced the efflux pump inhibition effect compared to the extract or tetracycline alone (p<0.05 for both). These findings support the potential use of polyphenol-rich P. emblica extracts as adjuncts to conventional antibiotics in treating drug-resistant bacterial infections.
Uji Aktivitas Anthelmintik Ekstrak Etanol Daun Sirih Merah (Piper ornatum N.E.Br) dan Daun Sirih Hijau (Piper belte L.) Terhadap Cacing Ascaridia Galli Secara In Vitro. Herdini Herdini; Ana Yulyana; Wildan Pratama
SAINSTECH: JURNAL PENELITIAN DAN PENGKAJIAN SAINS DAN TEKNOLOGI Vol 34 No 4 (2024): Sainstech : Jurnal Penelitian dan Pengkajian Sains dan Teknologi
Publisher : Institut Sains dan Teknologi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37277/stch.v34i4.2183

Abstract

Sirih di Indonesia ada beberapa jenis, yang dibedakan berdasarkan bentuk daun, rasa dan aromanya yaitu sirih merah (Piper ornatum N.E.Br) dan sirih hijau (Piper betle L.). Pada tanaman daun sirih terdapat senyawa tanin yang mampu menghambat kerja enzim dan mengganggu proses metabolisme pencernaan pada cacing yang dapat menyebabkan kematian cacing.. Penelitian ini bertujuan untuk mengetahui aktivitas anthelmintik menggunakan ekstrak etanol 96% daun sirih merah dan sirih hijau pada konsentrasi 1%, 3%, dan 5%, dengan pirantel pamoat sebagai kontrol positif dan NaCl 0,9% sebagai kontrol negatif secara in vitro. Dari hasil penapisan fitokimia yang telah dilakukan menunjukkan bahwa serbuk dan ekstrak daun sirih hijau mengandung senyawa flavonoid, saponin, tanin, triterpenoid. Serbuk dan ekstrak daun sirih merah menunjukkan bahwa tanaman ini mengandung flavonoid, tanin, dan saponin. Jumlah cacing yang mati dicatat setiap jam dan selanjutnya dianalisis menggunakan uji Shapiro Wilk, Levene, Kruskal Wallis dan dilanjutkan dengan uji Mann Whitney. Hasil pengujian menunjukkan rata rata waktu kematian cacing dengan ekstrak daun sirih merah yaitu untuk konsentrasi 1% selama 92 jam, konsentrasi 3% selama 86 jam, konsentrasi 5% selama 79 jam dan ekstrak daun sirih hijau yaitu untuk konsentrasi 1% selama 45 jam 40 menit, konsentrasi 3% selama 31 jam, konsentrasi 5% selama 13 jam. Nilai LC50 dan LT50 dari ekstrak sirih merah dan ekstrak sirih hijau pada konsentrasi 1%, 3%, dan 5% memiliki aktivitas anthelmintik dengan nilai LC50 1,30% dan LT50 83 jam 25 menit 15 detik untuk sirih merah, LC50 2,81% dan LT50 18 jam 36 menit 48 detik untuk sirih hijau. Kata kunci : Anthelmintik, Piper ornatum N.E.Br, Piper betle L.
Evaluation of Physical Attributes and Efficacy of Lip Balm Formulated with Okra Fruit Extract (Abelmoschus esculentus (L.) Moench Fructus) Ana Yulyana; Alfioni Wulandari J.E; Saiful Bahri; Eko Mugiyanto
Journal of Natural Product for Degenerative Diseases Vol. 2 No. 1 (2024): JNPDD September
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v2i1.7452

Abstract

The okra fruit (A esculentus fructus) is renowned for its significant antioxidant properties, attributed to its rich content of bioactive compounds. Despite its high antioxidant activity, which has been extensively documented, its potential as a component in cosmetic formulations particularly lip balm preparation remains underutilized. This study seeks to address this gap by incorporating okra fruit extract into lip balm formulations and evaluating the resulting physical and functional attributes. Lip balm formulations were developed with three varying concentrations of Ethanol okra fruit extract: F1 (1%), F2 (3%), and F3 (5%). A series of comprehensive evaluations were conducted to assess the physical stability and performance of the lip balms, including organoleptic properties, homogeneity, pH, melting point, spreadability, and overall stability. Stability was monitored over a 28-day period at room temperature to simulate typical storage conditions. The antioxidant efficacy of the okra extract was quantified using the DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, which yielded an IC50 value of 10.06 µg/ml, reflecting its potent free radical scavenging capacity. In comparison, the lip balm formulations demonstrated higher IC50 values, with Formulation I (FI) at 46.35 µg/ml, Formulation II (FII) at 40 µg/ml, and Formulation III (FIII) at 30.23 µg/ml, indicating a reduction in antioxidant activity in the formulated products compared to the pure extract.. The lip balm formulations exhibited a distinct okra aroma, smooth texture, and uniform color variations ranging from light brown (F1), brown (F2), to dark brown (F3). pH values ranged from 5.40 to 5.81, and melting points were between 49.6°C and 52.9°C, all within the acceptable range as per SNI 16-4769-1998 standards. ANOVA statistical analysis revealed no significant changes (p > 0.05) in melting point and spreadability over time. However, significant differences (p < 0.05) were noted in pH values, with further analysis indicating that formulations F2 and F3 differed significantly from F1 (p < 0.05). These results demonstrate that okra fruit extract not only enhances the antioxidant properties of lip balm but also maintains its stability and functionality, supporting its potential as a valuable ingredient in cosmetic formulations.
Development and Optimization of Facial Wash Gel with Okra Fruit (Abelmoschus esculentus) Extract: a Study on HPMC Concentration Variations Ana Yulyana; Annisya Maulidia Putri; Eko Mugiyanto
Journal of Natural Product for Degenerative Diseases Vol. 2 No. 2 (2025): JNPDD March
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v2i2.8392

Abstract

The stability of facial wash gel formulations is influenced by the concentration of Hydroxypropyl Methylcellulose (HPMC), which plays a crucial role in determining viscosity, homogeneity, spreadability, and foam stability. This study aimed to evaluate and optimize the physical stability of facial wash gel containing okra extract 3% (Abelmoschus esculentus (L.) Moench) by varying HPMC concentrations (1, 1.5, and 2%). The formulations were assessed for homogeneity, syneresis, pH stability, viscosity, spreadability, and foam stability over a 28-day storage period at room temperature (25°C). Statistical analysis was performed using SPSS version 27, including Shapiro-Wilk normality test, Levene’s test for homogeneity, One-Way ANOVA, and Tukey’s post-hoc test to determine significant differences among formulations. The results showed that HPMC concentration significantly influenced gel stability. F1 (1% HPMC) exhibited syneresis, excessive spreadability, and lower viscosity, indicating poor structural integrity. F2 (1.5% HPMC) maintained moderate stability but showed significant changes in spreadability over time. F3 (2% HPMC) demonstrated the best stability with optimal viscosity, minimal syneresis, controlled spreadability, and consistent foam formation. pH values for all formulations remained within the acceptable range (4.5–7.8, SNI 16-4380-1996). Foam stability (60–70%) met regulatory standards (SNI 16-4085-1996), with higher HPMC concentrations contributing to increased foam retention. The study confirms that HPMC concentration is a key determinant of gel stability. F3 (2% HPMC) exhibited the most stable formulation, balancing viscosity, spreadability, and foam stability, making it the optimal formulation for facial wash gel development. These findings highlight the importance of gelling agent optimization in cosmetic formulations, ensuring both product stability and consumer acceptability.
Antioxidant and In Vitro Antidiabetic Properties of Lansium domesticum Leaves Extracted with Solvents of Varying Polarity Situmorang, Jiro Hasegawa; Yulyana, Ana; Astyka, Ririn; Syahputra, Hafid; Lubis, Muhammad Fauzan
Indonesian Journal of Chemistry Vol 26, No 2 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.110695

Abstract

Conventional therapies for diabetes mellitus, such as oral hypoglycemic agents, are often limited by side effects and incomplete glycemic control, highlighting the need for safer alternatives. Exploring natural remedies, such as Lansium domesticum, is compelling, as this plant has been traditionally used for diabetes therapy. This study aimed to investigate how solvent polarity and affinity influence phytochemical content, antioxidant activity, and enzyme inhibitory potential of L. domesticum leaf extracts. Extraction was conducted using methanol, ethanol, and acetone (50%, 75%, 100%), and distilled water. Total phenolic content (TPC) and total flavonoid content (TFC) were quantified, and antioxidant activities were assessed via total antioxidant activity (TAA), DPPH, and FRAP assays. Antidiabetic activity was evaluated in vitro through α-glucosidase and α-amylase inhibition assays. The results indicated that 100% ethanol extract exhibited the highest TPC and TFC, correlating strongly with superior antioxidant and enzyme inhibitory activities. The extract has also demonstrated the most potent inhibition of α-glucosidase and α-amylase, with IC50 values of 70.64 and 105.13 µg/mL, respectively. Pearson correlation analysis revealed strong negative correlations between phytochemical contents and IC50 values. Overall, ethanol proved to be the most effective solvent for extracting bioactive compounds from L. domesticum leaves, underscoring its potential as a natural antioxidant and antidiabetic agent.
Co-Authors Alfioni Wulandari J.E Annisya Maulidia Putri Astyka, Ririn Daulay, Wardiyah Eko Mugiyanto G. Gunawan Hafid Syahputra Hendig Winarno Herdini Herdini Kaban, Vera E. Kosasih Lili Musnelina Lubis, Muhammad F. lubis, muhammad fauzan Munawarohthus Sholikha Nasution, Haris M. Nerry Miellana Nuke Paramitha Dewanti Ratna Hidayah Herawati Refdanita Refdanita Roy Indrianto Bangar S Satrio Satrio Ari Hutomo Situmorang, Jiro Hasegawa Syafriana, Vilya Utama, Rezza F. Wildan Pratama