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All Journal Jurnal Teknologi Dan Industri Pangan Teknotan: Jurnal Industri Teknologi Pertanian Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat agriTECH Agricore: Jurnal Agribisnis dan Sosial Ekonomi Pertanian Unpad ASIAN JOURNAL FOR POVERTY STUDIES (AJPS) Chimica et Natura Acta Journal of Industrial and Information Technology in Agriculture Jurnal Mutu Pangan : Indonesian Journal of Food Quality Jurnal Agro Dedikasi Masyarakat (JADM) Media Penelitian dan Pengembangan Kesehatan
Robi Andoyo
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Journal of Industrial and Information Technology in Agriculture

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Ozonation Pretreatment Evaluation for Xylanase Crude Extract Production from Corncob under Solid-State Fermentation Efri Mardawati; Surya Martha Pratiwi; Robi Andoyo; Tita Rialita; Mochamad Djali; Yana Cahyana; Een Sukarminah; In-In Hanidah; Imas Siti Setiasih
Journal of Industrial and Information Technology in Agriculture Vol 1, No 2 (2017): DECEMBER 2017
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.181 KB) | DOI: 10.24198/jiita.v1i2.14664

Abstract

Xylanases are highly exploited enzyme in industries, including food and chemical industry. Xylanases can be utilized in catalyzing the endohydrolysis of 1,4-β-xylosidic linkages in xylan, lignocellulosic component to produce xylose-monomer. This research aims to optimize xylanase production from alternative substrate, corncob. Corncob contains 41.17% of hemicellulose, polymer of xylan. Xylanases are produced through solid state fermentation by Trichoderma viride. Ratio between substrate and moistening solution was 0.63 g/mL with fermentation temperature 32,8OC. Variables varied include incubation time and pretreatment (using autoclave, ozonation, combination of ozonation and autoclave, also without pretreatment). Xylanase activity was measured by DNS method using 1% of xylan as substrate standard. The result showed that the best incubation time is 36 h with 14403.8707 U/mg protein for specific xylanase activity by using autoclave as pretreatment.  Ozonation pretreatment process can increase the enzyme activity of xylanase.
Co-Authors A.A. Ketut Agung Cahyawan W Adhimah, Ayu Fauziyyah Agung Tri Mulyono Ani Margawati Anindya Rahmana Fitri Asriyanti Septiani Gunawan Bambang Nurhadi Diana Nur Afifah, Diana Nur Edy Subroto Een Sukarminah Efri Mardawati Endah Wulandari Endrinikapoulos, Ariana Etika Ratna Noer Hadi, Bambang Nur Ihat Hatimah Imas Siti Setiasih In-In Hanidah Intan Btari Dwiastuti Irza, Damar Khairina, Zahidah Maria Mexitalia Marleen Sunyoto Mochamad Djali Mohamad Djali Mohammad Djali Mulyono, Agung Tri Nandi Sukri Nurseto, Hardian Eko Nuryanto Nuryanto Pangawikan, Aldila Din Ramadanti Fitra Ratih Siswanina Putri Rossi Indiarto Rudi Saprudin Darwis, Rudi Saprudin Samsul Huda Samsul Huda Siti Nurhasanah Slamet Hadi Kusumah Souvia Rahimah Sumanti Debby Moody Surya Martha Pratiwi Syamsul Huda Tita Rialita Tita Rialita Triani, Rini Yana Cahyana